Studies on degradation mechanism of niclosamide ethanolamine salt Ⅰ Determination of niclosamide ethanolamine salt in photolysis solution

目的了解氯硝柳胺乙醇胺盐(nES)在水体中的光解规律。方法实验室配置PH5的0.05μg/Ml、PH7的0.5μg/Ml和PH9的2.5μg/MlnES溶液,采用氙灯光源作为模拟日光照射,照射后0、0.5、1、2、4、8、16、24、48、72、96H采集光解液,高效液相色谱法测定光解液中nES含量,同时设无光照对照试验,计算其半衰期。结果在氙灯光光照条件下,nES快速降解,8H3种PH值的nES溶液下降率分别为83.4%、78.8%和41.3%,24H下降率分别为92.1%、88.5%和95.8%。nESPH5半衰期为8.98H,PH7为10.34H,PH9为9.16H;无光照条件下放置12d溶液浓度稳定。结论nES可在水中快速光解。Objective To understand the photolysis mechanism of niclosamide ethanolamine salt(NES) in water body.Methods NES was formulated into solutions of pH 5(0.05 μg/ml),7(0.5 μg/ml) and 9(2.5 μg/ml),respectively.The xenon gas lamp was used as the simulated sunlight.After 0,0.5,1,2,4,8,16,24,48,72,96 h of the illumination,the photolysis solutions were collected to determine the amount of NES by using the high-performance liquid chromatography method(HPLC),and the experiment without illumination was also set as the control.The half-life was calculated.Results Under the illumination of xenon gas lamp,the NES degraded rapidly.The degradation rates were 83.4%,78.8% and 41.3%,respectively for 8 h,and 92.1%,88.5% and 95.8%,respectively for 24 h.The half-life of NES was 8.98 h for pH 5,10.34 h for pH 7,9.16 h for pH 9.The solution could keep stable for 12 d without illumination.Conclusion Photolysis of NES can occur quickly in water.江苏省自然科学基金(BK200602

Studies on degradation mechanism of niclosamide ethanolamine salt Ⅱ Isotopic tracing and determination of photolysis end-product

目的观察同位素示踪技术解析氯硝柳胺乙醇胺盐(nES)在水体中的稳定性及测定光解终产物CO2的变化。方法实验室配制PH5的0.05μg/Ml、PH7的0.5μg/Ml和PH9的2.5μg/MlnES溶液,采用氙灯光源作为模拟日光照射上述nES溶液,照射8、16、24、32、40、48H和72H后,分别测定nES溶液在光解管顶的气态样品,采用gASbEnCHⅡ进样针插入直接分析;液体样品则取2.5Ml注入用高纯氦吹过的密封顶空样品瓶中,用加酸泵滴加无水磷酸,30℃超声反应1H,再插入gASbEnCHⅡ进样针进行分析;与dElTAPluS/XP稳定同位素质谱仪联用测定光解产物CO2的量及碳稳定同位素。结果在氙灯光光照条件下,PH70.5μg/Ml和PH92.5μg/MlnES溶液的光解,随光照时间的增加,CO2的量也增加,并呈线性增长,72H时其产量接近nES的实际产量。同位素结果显示,随光照时间的增加,CO2的碳同位素值逐渐变负,72H时接近nES原药的碳同位素值,为-25.36±0.11,光降解已接近完全。而PH50.05μg/MlnES溶液的光解,随光照时间的增加,CO2的量也在增加,但其产量超出了nES的实际产量;同位素结果显示,随光照时间的增加,产出CO2的碳同位素值逐渐变负,但在光照16H后其产CO2的碳同位素值比nES的碳同位素值偏负。结论 nES能够在水中快速光解,终产物为CO2。Objective To analyze the stability of niclosamide ethanolamine salt(NES)in water and determine the changes of CO2,the photolysis end-product,by using the isotopic tracing technique.Methods NES was formulated into the solutions of pH 5(0.05 μg/ml),pH 7(0.5 μg/ml)and pH 9(2.5 μg/ml),respectively.The xenon gas lamp was used as the simulated sunlight.After 8,16,24,32,40,48,72 h of the illumination,the gas samples of the NES solution on the top of photolysis tube were determined by using direct insertion of GasBenchII needle.For liquid samples,2.5 ml NES solution was added into a sealed empty bottle,which was treated with high pure helium,and then anhydrous phosphoric acid was added.After ultrasonic reaction at 30 ℃ for 1 h,the GasBenchII needle was inserted for analysis.The amount of CO2 and carbon isotopic value were determined by using the GasBenchII needle combined with isotopic mass spectrometer.Results Under the illumination of xenon gas lamp,the NES solutions of pH 7 and pH 9 occurred photolysis,with the increase of illumination time,the amount of CO2 increased,showing a linear increase.At 72 h,its yield approximated to the active output of NES.The isotopic tracing results showed,with the increase of illumination time,the carbon isotope value of CO2 turned to be negative gradually,and approximately reached to the carbon isotope value(-25.36±0.11‰)of NES active ingredient at 72 h when the photolysis approximated to be completely finished.For the photolysis of pH 5 NES solution,with the increase of illumination time,the amount of CO2 increased,however,its output was more than the active output of NES.The isotopic tracing results showed,with the increase of illumination time,the carbon isotope value of CO2 turned to be negative gradually.However,after illumination for 16 h,the carbon isotope value of CO2 was more negative than that of NES.Conclusions The photolysis of NES can occur rapidly in water,and the end product is CO2.江苏省自然科学基金(BK200602);江苏省卫生厅项目(X200504

基于内毒素血症探讨皂术茵陈方对非酒精性脂肪性肝炎大鼠的影响

目的 基于内毒素血症探讨皂术茵陈方防治非酒精性脂肪性肝炎(NASH)大鼠的影响及其作用机制。方法 采用随机数字表法将40只大鼠分为5组,即正常组、模型组、皂术茵陈方组、盐酸吡格列酮组及培菲康组,每组8只。除正常组外,其余32只大鼠采用高脂饮食16周建立大鼠NASH模型,在造模第9周开始,皂术茵陈方组给予皂术茵陈方水提液60 mg/(kg·d)灌胃,盐酸吡格列酮组给予盐酸吡格列酮10 mg/(kg·d)灌胃,培菲康组给予培菲康210 mg/(kg·d)灌胃,正常组及模型组均给予双蒸水10 mL/(kg·d)灌胃,共治疗8周。第16周末经腹主动脉取血,生化法检测肝组织甘油三酯(TG)水平,苏木精—伊红(HE)染色观察肝组织病理学变化。终点显色法检测血浆内毒素(LPS)含量。ELISA法检测肝组织肿瘤坏死因子-α(TNF-α)、白细胞介素(IL)-1β、IL-6等炎症因子的表达水平。结果 与正常组比较,模型组大鼠肝组织显示出典型的NASH组织学特征,出现重度脂肪变性,不同程度的炎细胞浸润和坏死灶。大鼠肝湿重、肝指数、肝脏TG含量、血浆LPS水平、肝组织TNF-α、IL-1β、IL-6等炎症因..

活心丸(浓缩丸)治疗冠心病稳定性心绞痛的多中心、随机、双盲、安慰剂对照临床研究

目的观察活心丸(浓缩丸)治疗冠心病稳定性心绞痛的临床疗效和安全性。方法本研究采用多中心、随机、双盲、安慰剂对照研究方法,将480例气虚血瘀型冠心病稳定性心绞痛患者以3:1比例随机分为试验组(360例)和对照组(120例)。在西医常规治疗基础上,两组患者分别服用活心丸(浓缩丸)和安慰剂,1次2粒,每日3次;疗程8周。观察两组治疗前后主要指标(心绞痛症状积分)和次要指标(包括硝酸甘油减停率、中医证候积分及生活质量评价)及安全性。结果共454例患者完成试验(试验组336例,对照组118例)。与本组治疗前比较,试验组和对照组心绞痛症状积分和中医证候积分均降低,西雅图心绞痛量表评分改善(P<0.01);与对照组治疗后比较,试验组心绞痛症状积分、中医证候积分、西雅图心绞痛量表评分改善均优于对照组(P<0.01)。试验组心绞痛症状疗效总有效率为80.95%,硝酸甘油停减率为80.70%,中医证候疗效有效率80.65%,均高于对照组[36.44%、45.07%、38.99%(χ~2=58.21、40.94、66.55,P<0.01)]。试验组中既往有心梗史患者心绞痛症状总有效率为83.22%,中医证候有效率83.22%,西雅图心绞痛量表评分为(361.74±62.10)分,均高于无心梗史患者[60.92%、66.89%、(327.95±65.07)分(χ~2=13.89、13.26,P<0.01)]。治疗过程中未发生明显不良反应。结论活心丸(浓缩丸)治疗气虚血瘀型冠心病稳定性心绞痛疗效显著,尤其适用于既往有心梗病史的冠心病稳定性心绞痛患者,无明显不良反应。国家中医药管理局资助课题(No.国中医药科2016ZX04

Reduced-order H_∞ Filtering Design for Singular Systems Based on Stable Invariant Zeros

研究了连续奇异系统的降阶H∞滤波器的设计问题.通过将原系统转化为一个标称系统和一个不确定部分,同时增加补充的约束条件,使原系统的稳定不变零点转化为标称系统的不稳定不变零点,从而可以基于标称系统,利用已有的降阶算法设计得到原系统的降阶H∞滤波器.文中给出了降阶H∞滤波器的设计算法,并进一步研究了可用于构造降阶滤波器的不变零点的范围.最后给出一个算例,说明该设计方法的有效性和可行性.This paper considers reduced-order H∞ filtering design for continuous singular systems.By adding a complementary constraint,the original system is divided into a nominal system and an uncertain part,then the stable invariant zeros of the original system become unstable in the nominal system.Based on the nominal system,a reduced-order H∞ filter for the original system can be designed by using the existing method.An algorithm to solve the problem of reduced-order H∞ filtering is proposed,and the range of invariant zeros which can be used to construct a reduced-order filter is discussed.An illustrative example is given to demonstrate the effectiveness of the proposed approach.福建省新世纪优秀人才支持计划资

编制中国人个性测量表(CPAI)的意义与程序

从1990年开始中国科学院心理研究所与香港中文大学心理系合作,编制&ldquo;中国人个性测量表&rdquo;(CPAI)。编制方法严格按照个性测验编制程序进行。共分四个步骤,一、建立正常与病态个性量表的框架。二、构成量表的项目。三、测量表的最后确定。四、量表的标准化工作。编制出的测量表共包括22个正常个性量表,12个病态个性量表及2个效度量表,共510个项目。经实践检验与统计分析,证明该测量表具有一定的信度与效度。</p

Effects of Psychological Intervention on Immunological Function in Cancer Patients

目的:通过心理干预前后免疫参数的变化,了解心理干预能否提高癌症患者的免疫功能。方法:运用病例对照实验设计,采用多因素综合分析统计方法,对120例(采集到血样者81例)患者进行比较研究。结果:化疗期间经心理干预后,患者的各项免疫参数均有不同程度的提高; NK细胞活性对心理行为的变化比较敏感,干预后明显提高; 实验组免疫球蛋白三项指标化疗前后比较稳定,但对照组化疗后明显下降。癌症患者的愤怒表达和掩饰性性格特点与NK细胞活性显著相关; 影响免疫功能的因素随免疫参数的不同而异; 各种影响因素以综合作用为主,部分因素间交互作用明显。结论:心理干预对提高癌症患者的免疫功能起到一定作用,但其机制和稳定性有待进一步研究

双台河口国际重要湿地芦苇地上生物量遥感估算

基于多时相的Landsat8 OLI卫星遥感数据,采用面向对象的分类方法,提取双台河口国际重要湿地芦苇分布信息。通过对归一化植被指数(normalized difference vegetation index,NDVI)等6个植被指数与野外实测芦苇地上生物量数据间的统计分析,比较不同植被指数对芦苇地上生物量的敏感性,构建双台河口国际重要湿地芦苇地上生物量遥感反演模型; 应用该模型对芦苇地上生物量进行遥感反演以及空间格局分析。结果表明: 双台河口国际重要湿地芦苇分布面积为4.39×10~4 hm~2,约占该研究区总面积32.96%; 选取的6个植被指数均与芦苇地上生物量显著相关(p<0.05),其中,以NDVI为变量的幂指数形式的估算模型为芦苇地上生物量遥感估算最优模型,模拟精度为79%,决策系数为0.76; 双台河口国际重要湿地芦苇地上生物量呈东高西低和北高南低的分布格局,其平均地上生物量为4 785.5 g/m~2,总地上生物量为2.06×10~6 t; 本研究结果可为双台河口国际重要湿地生态系统管理和生物多样性保护提供数据支持与科学指导

The Mediating Effects of the Coping Styles and Related Factors on Psychological Intervention in Cancer Patients

目的:探讨在对癌症患者实施心理干预过程中应对策略及其相关因素的中介作用.方法:运用病例对照实验设计,对实验组病人进行了为期3个月的心理干预,选用疾病应对问卷作为主要测查工具,采用多因素综合分析统计方法,对120名住院化疗病人进行了比较分析.结果:干预组病人在干预后较多运用主动认知和主动行为策略,较少运用回避方法,对照组则变化不大;干预后病人情绪状态、功能状况以及生活质量的改善与所采用的应对方法有一定关系: 与主动认知应对方法呈正相关,与回避性应对方法呈负相关,与主动行为应对方法则关系不明显.结论:应对策略及其相关因素在癌症患者心理干预中起一定的中介作用