261 research outputs found

    Function of SAV6 in Maintenance of Genome Integrity and Root Development in Arabidopsis

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    flap核酸内切酶FEN1(flapendonuclease1)是RAD2家族的一种能够识别特异底物结构的核酶。FEN1具有三种酶活性:flap核酸内切酶(FlapEndonuclease,FEN)活性、5’核酸外切酶(5’-Exonuclease,EXO)活性和缺口依赖的核酸内切酶(GapEndonuclease,GEN)活性,在DNA复制、修复以及维持端粒稳定性等方面发挥着重要作用。人们对FEN1在哺乳动物、酵母中的功能已经有了深入了解,但是拟南芥中尚无相关研究。 我们在筛选遮荫反应相关基因的突变体时筛到了一个sav6(shadeavoidance6)突变体,表现为下胚轴和根短小。通过图...FEN1 (Flap endonuclease1) belongs to Rad2 nuclease family,it recognizes the DNA substrate with special structure. It possesses 3 kinds of enzyme activity: flap endonuclease activity, exonuclease activity and gap endonuclease activity. It is required for several DNA metabolic pathways, including DNA replication, repair and maintenance of telomere stability. Despite the wealth information available ...学位:理学博士院系专业:生命科学学院_生物化学与分子生物学学号:2162010015390

    玻璃浆料薄膜的微接触转印制备

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    在使用玻璃浆料对MEMS器件进行封装的过程中,中间层即玻璃浆料薄层的厚度、宽度等参数会对封装强度和气密性等产生至关重要的影响。为了有效控制玻璃浆料层的宽度、厚度,文中首次研究了利用微接触转印方法进行玻璃浆料薄膜的制备。在对微接触转印工艺流程和微接触转印装置的优化设计之后,采用了不同厚度的凸模进行了玻璃浆料的微接触转印实验。实验结果表明:通过微接触转印可以制得表面光滑边缘整齐的玻璃浆料薄膜,其宽度随着凸模厚度的减小而减小,厚度基本保持不变;在凸模厚度为70μm时,获得了玻璃浆料薄膜的宽度和厚度分别为200μm和52μm,可控性较高

    消费品价格与城市化进程——以食品价格为例的实证分析

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    肉禽等消费品价格的上涨源于城市化进程的加剧、饲料价格上涨以及人均耕地面积下降。本文利用协整检验和格兰杰因果检验等方法对猪肉价格和相关变量进行实证分析。最后提出了加大农业投入、建立食品价格预警机制等一系列政策建议来消除消费品价格的非正常上涨

    Innovation projects management of company A: System Optimization and effectiveness of the Case

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    在充满竞争的环境下,企业创新管理成为管理层不能忽视的核心工作。创新项目则是企业创新活动最为常见,也是最为重要的载体和表现形式。创新项目管理的核心在于如何实现创新项目的系统、有序开展,并使之与企业日常运营活动实现价值融合,从而实现战略目标,保障战略规划的全面实现。如何建立有效的创新项目管理系统,已经成为企业管理理论与实务不可回避的问题。本文尝试性地围绕这一主题开展研究,提出解决问题的系统性框架。 在以上研究主题下,本文解决问题的基本思想是构建以战略为导向的创新项目管理体系。这一体系的基本特征是:以企业价值创造过程为载体,紧密围绕战略目标,创新配置和使用企业资源,通过系统的管理流程与原则,实现创...In this competitive environment, innovation management has become the core task of company managers which can not be ignored anymore. In a company, innovation projects are not only the most common, but also the most important vehicles and forms of the innovation activities. The core of innovation project management is how to implement innovative projects system and integrate with the daily operat...学位:工商管理硕士院系专业:管理学院高级经理教育中心(EMBA项目)_高级管理人员工商管理硕士(EMBA)学号:X200615609

    PKFSKC: PCA Based Key Frame Similarity Kernel Clustering Algorithm

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    针对基于内容的视频检索领域中,关键帧特征矩阵维度不同时的相似度计算问题,提出一种基于主成分分析的关键帧相似度核聚类检索算法。首先,针对任意具有不; 同数量关键帧的视频片段,提取特征向量并构造不同维度的特征矩阵。其次,基于PCA计算对特征矩阵进行SVD计算降维矩阵后,结合矩阵运算方法及核方法设; 计出一种视频关键帧相似度核聚类检索算法,并给出其加权改进形式。最后,通过测试视频标准库和人工视频片段的实验表明,该算法能更好地提视频高视频检索的; 效率。In the content-based video retrieval research, a PCA based key frame; similarity kernel clustering algorithm is proposed to calculate the; similarity of the feature matrix of video key frame with different; dimensions. Firstly, feature vectors and structure feature matrices with; different dimensions of any different video clip key frame are; extracted. Secondly, the dimension reduction matrix with SVD method; based on PCA algorithm is calculated, the key frame similarity kernel; clustering algorithm is proposed with the matrix calculation method and; the kernel method, and its improved weighted representation is proposed; as well. Finally, the simulation experiments on the standard test video; database and artificial video clip database show that the algorithm can; improve the efficiency of video retrieval.福建省软科学一般项目; 2016年虚拟现实技术与系统国家重点实验室立项; 厦门大学立项课题项

    Determination of Gallic Acid Content in Extract of Canarium album (Lour.) Raeusch Fruit and Its Radical Scavenging Activity

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    采用高效液相色谱法测定橄榄果实甲醇提取物中没食子酸的含量,并用二苯基苦基苯肼自由基(dPPH.)法研究了橄榄果实提取物的自由基清除能力。没食子酸质量浓度在8.4--42 Mg/l范围内,没食子酸峰面积与其进样量呈良好的线性关系(r=0.999 3),没食子酸平均质量分数4.03%,相对标准偏差(rSd)(n=6)1.77%,平均回收率98.75%,rSd(n=6)1.21%。该方法简单灵敏,准确可靠,重复性好。橄榄果实甲醇提取物具有较高的自由基清除能力,dPPH.的半数抑制浓度(IC50)为40.14 Mg/l。The content of gallic acid in extract of white canarytree(Canarium album(Lour.)Raeusch) fruit was determined by HPLC.The radical scavenging activity of extract was determined by 1,1-diphenyl-2-picrylhydrazyl(DPPH) assay.The linearity of gallic acid content was over the range of 8.4-42 mg/L with good linear correlation(R=0.999 3).The mean value of gallic acid content was 4.03 %,with relative standard deviation(RSD)(n=6)1.77 %.The average recovery was 98.75 % with RSD(n=6)1.21 %.The determination method is simple,sensitive,accurate,reliable and with good reproducibility.C.album fruit extract showed a very good DPPH radical scavenging activity(IC50) 40.14 mg/L.国家自然科学基金资助项目(30671646);教育部新世纪优秀人才支持计划项目(NCET-07-0725

    Preparation and Antioxidant Activity of Cyanidin from Condensed Tannins Extracted from Calyx of Bruguiera gymnorrhiza

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    利用MALDI-TOF MS分析了红树植物木榄花萼缩合单宁的结构单元组成类型、平均聚合度和平均分子质量。并以木榄花萼缩合单宁为原料,通过HCl-正丁醇法酸解反应制备了花青定粗产品,同时测定了花青定粗产品对二苯基苦基苯肼自由基(DPPH.)的清除能力及铁离子还原/抗氧化能力(FRAP)。结果表明:木榄花萼缩合单宁结构单元组成类型主要为儿茶素或表儿茶素,平均聚合度为7.5,平均分子质量为2081.60u;花青定粗产品具有较强的清除自由基能力,DPPH.半数抑制浓度(IC50)为43.889mg/L,并具有较高的FRAP((7.718±0.109)mmol/g)。Type of structural units,degree of polymerization and mean molecular weight of condensed tannins extracted from calyx of large red mangrove (Bruguiera gymnorrhiza) were characterized by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). Crude cyanidin products were prepared from condensed tannins of B. gymnorrhiza by means of butanol/HCl reaction. In addition,the free radical-scavenging activity and antioxidant ability of crude cyanidin products were determined by using 1,1-diphenyl-2-picrylhydrazyl radical (DPPH·) and ferric reducing/antioxidant power (FRAP) model systems respectively. The results showed that catechin/epicatechin were the structural units occurring in condensed tannins of B. gymnorrhiza. Average degree of polymerization was 7.5,and mean molecular mass was 2 081.60 u. Crude cyanidin products showed a very good DPPH radical scavenging activity (IC50 of 43.889 mg/L) and ferric reducing/antioxidant ability ((7.718± 0.109)mmol/g).国家自然科学基金(30671646);; 教育部新世纪优秀人才支持计划(NCET-07-0725

    钛板骨内固定系统在颌骨骨折中的应用研究

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    目的 :通过动物实验及临床研究评价钛板颌骨内固定系统的临床应用效果。方法 :实验测试了内固定系统的机械性能和组织反应性。临床对 167例颌骨骨折病例应用钛板颌骨内固定治疗 ,观察咬牙合关系、咀嚼功能、开口度、面部畸形等恢复情况。结果 :微型接骨板桡曲强度偏低、钛板、钛钉表面耐磨性差。 167例临床病例治疗效果可靠。结论 :该固定系统设计合理 ,应用于颌骨骨折的治疗疗效可
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