The Histochemical and Ultrastructural Study of Anther Development in Rhododendron L

本实验应用细胞组织化学和透射电镜技术，对杜鹃花药发育中营养物质的代谢积累特点和超微结构特征分别进行研究，主要实验结果如下： 1.营养物质的代谢变化 杜鹃花药发育中营养物质分布规律如下：在造孢细胞时期，绒毡层细胞和初生造孢细胞中都有多糖颗粒。在形成胼胝质壁的小孢子母细胞质中只含有少量脂滴，在绒毡层细胞质中分布较多的多糖颗粒。四分体时期，绒毡层细胞的多糖颗粒明显增多，四分体小孢子中只含有少量的脂滴。到小孢子早期，绒毡层细胞和小孢子中都只存在很少量脂滴，发育到小孢子晚期，即单核靠边期，绒毡层细胞开始退化。在二胞花粉早期，药室内壁细胞质中积累了很多多糖颗粒，花粉粒细胞质中分布很多多糖颗粒和脂滴。成...At present , the ultrustructural features of developing anthers of Rhododendron were studied using transmission electron microscope technique. The characteristics of distribution of nutriment during the anther development were also studied using cytochemical technique. The results as following: 1 The nutriment accumulation during the anther development Anthers of Rhododendron display some ...学位：理学硕士院系专业：生命科学学院_发育生物学学号：2162011115248

Density and Biomass of Meiofauna in the Taiwan Strait and its Adjacent Waters

小型底栖动物数量巨大，种类繁多，是许多经济鱼、虾和贝类幼体阶段的优质饵料，在海洋生态系统中起着重要作用。深入进行相关研究，对于了解海洋底栖生态系统的结构和功能，建立生态动力学模型和探讨水层-底栖耦合机制具有十分重要的意义。 2006年7月（夏季）、12月（冬季）和2007年4月（春季）、10月（秋季），对台湾海峡及邻近海域25个站位进行四个航次小型底栖动物调查研究，对研究海域小型底栖动物的沉积环境、类群组成、密度和生物量季节变化、群落结构和多样性等进行研究。研究结果如下： 研究海域调查站位平均水深为41m，沉积物类型以砂质粉砂（ST）和粘土质粉砂（YT）为主。四个航次共挑出30846个样品...Meiofauna are species-richness and numerous in the ocean. They are the food of fish, shrimp and crab, and they play an important role in the marine food chain. Miofauna research is an important component of marine biological survey. The study of meiofauna is significant to understand the marine benthic ecosystem structure and function. The studies of meiofauna in the Taiwan Strait and its adjace...学位：理学硕士院系专业：生命科学学院生物学系_水生生物学学号：2172006115213

明清江西萧公、晏公信仰入黔考

本文以贵州地方志为基本资料,考察了明清时期江西地方性神祇萧公、晏公信仰在贵州的分布、传播情况;进而探讨了它们由乡土之神转变为他乡之神的动力问题;认为萧公、晏公信仰的广泛传播,与明清时期移民社会的大背景相关。江西移民、商人进入贵州的同时也移植了自己的信仰,成为推动萧公、晏公信仰在贵州广泛分布的主要力量;同时,国家的支持也起到了推动作用。江西省社科规划青年项目“江西水神萧公信仰的建构与传播研究”(项目编号:13LS14)阶段性成

Determination of Estrogenic Compounds in Water of Jiulong River Using Polar Organic Chemical Integrative Sampler

极性有机物一体化采样器(POCIS)作为富集水体中有机物的新型采样技术,可以反映目标物在被测水体中的时间权重浓度,因此在环境监测中是对主动采样方式的重要补充.本研究采用POCIS进行了4种固醇类雌激素化合物(雌酮、雌二醇、雌三醇及雌炔醇)的富集动力学,以及温度和目标物浓度对POCIS富集雌激素影响的实验室模拟研究.结果表明,POCIS对目标化合物的富集放置时间7 d内呈现显著的线性关系(r2≥0.988 1,P12%),随着温度的升高,目标化合物在POCIS上的采样速率减小.而目标物浓度对POCIS富集没有显著的影响(rSd 12%).As for different concentrations of target compounds,the sampling rate was not affected by the concentrations( RSD < 5%).After laboratory experiments,an environmental field study was performed in Jiulong River.The results showed that there was a relatively good correlation between the measured and calculated values( r2= 0.720 9,P < 0.001).It demonstrated that POCIS can be used in aquatic field systems.近海海洋环境科学国家重点实验室访问学者基金项目; 海洋公益性行业科研专项(201105013

Separation of Sperm Cells of Rhododendron simsii

作者简介：张玉红(1988-)，女，山东乳山人，硕士研究生，从事植物学研究。E-mail: [email protected] 注：田惠桥为通讯作者。E-mail: [email protected]杜鹃成熟花粉为二胞型,含一个营养细胞和一个生殖细胞,其精细胞在花粉管中形成。应用半离体技术培养杜鹃已授粉花柱,使花粉管从花柱中长出,再用渗透压冲击法促使花粉管破裂,释放出一对与营养核相连的精细胞。分离的精细胞经FDA方法检测,证明具活性。用显微操作仪可收集数量较多的分离精细胞。[英文文摘]The mature pollen of Rhododendron simsii is bicellular, containing one vegetative cell and one germ cell, with the sperm cells forming in pollen tube. Semi vitro technique was used in style pollinated to make pollen tube sprout from style. Then the pollen tube burst by osmotic shock method and released a pair of sperm cells which connected to vegetative nucleus. The sperm cells separated kept activities proved by FDA method. A fairly large number of separated sperm cells could be collected by micromanipulator.国家自然科学基金(30970275、31170289);公益性行业(农业)科研专项课题(200903016)资

Preparation and in vitro evaluation of chitosan microspheres containing matrine

目的:以壳聚糖为囊材制备苦参碱结肠靶向给药微球及评价其体外释药情况。方法:用乳化化学交联法制备微球,以微球的粒径分布百分数、载药量及包封率为优化指标对影响微球制备的主要因素用正交试验设计优化制备条件;并对最佳制备工艺制得的微球进行3种不同递质(人工胃液、人工肠液及大鼠结肠液)中的体外释放度评价。结果:制得的苦参碱壳聚糖微球在电镜下,球形表面圆整,粒径分布适宜,微球平均粒径为(68.3±2.7)μm,平均载药量为(16.0±0.5)%,平均包封率为(66.3±4.2)%。苦参碱壳聚糖微球在人工胃液中2h不释药;在人工肠液中4h内释放不到1%,96h释药不到10%;在含大鼠结肠内容物的磷酸盐缓冲液(pH6.8)中4h释放10%左右,36h释药近50%,此后释药趋于缓慢,96h释药近80%。结论:苦参碱壳聚糖微球几乎不在上消化道释药,而是在结肠靶向释药。OBJECTIVE To preparate the chitosan microspheres containing matrine for the colon-specific drug delivery and evaluate its release in vitro.METHODS The chitosan microspheres containing matrine were prepared by emulsion-chemical crosslink technique.The better preparation procedure with respect to particles size distribution,embedding rate and drug loading were optimized by the orthogonal experimental design.In-vitro drug release were carried out in the artificial gastric,artificial intestinal and the phosphate buffered saline(pH(6.8)) containing rat colon contents.RESULTS The chitosan microspheres containing matrine was shown to have good spherical geometry,a smooth surface and suitable size distribution under scanning electron micrographs.Average diameter of microspheres was((68.3)±(2.7))μm,drug loading was((16.0)±(0.5))%,embedding rate ((66.3)±(4.2))%.Little release and a little release of matrine from the microspheres were observed in the artificial gastric juice and in the artificial intestinal juice,respectively.However,the release of matrine was markedly increased in the phosphate buffered saline(pH(6.8)) containing rat colon contents,which was near to 10%,50% and 80% at 4 h,36 h and 96 h,respectively.CONCLUSION These findings suggest that the chitosan microspheres containing matrine may be useful a dosage form for colon-specific drug delivery.甘肃省自然科学基金(编号ZS0001-A23-073-Y

Phytoplankton biomarkers in surface seawater from the northern South China Sea in summer 2009 and their potential as indicators of biomass/community structure

生物标志物已被广泛应用于重建浮游植物生产力和群落结构变化。该方法假设之一是生标的含量可以反映表层海水的浮游植物生物量,但这个假设还缺乏现场观测的充分验证。对2009年冬季南海北部表层海水颗粒物中主要生标做了分析,利用其含量及比值研究浮游植物的生物量及群落结构的分布。生标含量表明硅藻、甲藻、颗石藻的高值区位于珠江口南部及广东沿岸,在陆坡区也有高值。生标比值显示硅藻在3个类群中的相对比例最高。其中,甲藻/硅藻比值高值区位于陆坡区,这与大洋水(黑潮)的入侵,带来大量暖水性甲藻有很大关系;颗石藻/硅藻比值总体趋势与生物量的分布相反,在近岸少数站位有高值,向外海逐渐增加,主要是因为颗石藻更适于寡营养盐的环境。生标结果所指示的生物量及群落结构的空间分布与前人的调查结果类似,为利用生标重建此区域的浮游植物生产力和群落结构变化提供了依据。Biomarkers have been widely used to reconstruct phytoplankton productivity based on the assumption that biomarker contents could reflect phytoplankton productivity in the surface seawater.However,this hypothesis has not been validated with modern survey.In this study,the contents and ratios of three phytoplankton biomarkers in the surface seawater of the northern South China Sea in winter 2009 were analyzed,to indicate spatial distributions of phytoplankton biomass and community structure.The results show high values for diatoms,dinoflagellates and coccolithophorids near the Pearl River Estuary and Guangdong coastal areas.The community structure indicates diatoms are the dominant phytoplankton species in winter.High values of dinoflagellate/diatom ratio occur near the slope area owing to intrusion of the Kuroshio,which favors the growth of dinoflagellates.The coccolithophorid/diatom ratio displays an increasing trend from the Pearl River Estuary to offshore region due to the advantage of coccolithophorids in oligotrophic environment,which is opposite to the productivity pattern of all biomarkers.These results are consistent with previous studies using phytoplankton cell and pigments,which provides support for the use of biomarker to reconstruct phytoplankton productivity and community structure in the SCS.国家重点基础研究发展计划项目(2007CB815934、2009CB421201);国家自然科学基金项目(40776029

聚合条件对乙烯-1-辛烯共聚反应及性能影响

以甲苯为溶剂,采用自制茂金属催化剂、助催化剂三异丁基铝[Al（iBu）3]和三苯碳四（五氟苯基硼）[Ph3C]+[B（C6F5）4]-催化乙烯与1-辛烯共聚,探究了聚合温度和1-辛烯浓度对乙烯-1-辛烯共聚反应以及共聚物结构与性能的影响。结果表明:当聚合温度从100℃升高到150℃时,催化活性下降,共聚物相对分子质量持续降低,其分布则变宽,熔融温度和结晶度均上升;当1-辛烯浓度从0升高到1.2 mol/L时,催化活性显著增大,共聚物相对分子质量分布变宽,熔融温度和结晶度均下降

Testing AGNPS for water quality modeling in agricultural catchment in southeast of China

利用地理信息系统 (GIS) ,基于数字高程模型 (DEM) ,提取AGNPS(AAgriculturalNon PointSourceModel)模型所需的水文和地形参数 ,并通过实地调查及专题制图等手段获取AGNPS模型所需的土地利用、土壤质地及施肥水平等其它参数 ,最后利用监测降水 ,实测水文参数、营养盐及沉积物负荷等验证AGNPS模型在我国东南亚热带地区的应用 .It is feasible to evaluate the Non-point Source pollution such as sediments, agricultural chemicals using water quality model in the basin scale. AGNPS (Agricultural Non Point Source), an event driven, distributed parameter model, which is developed and validated primarily in the United States, was employed in the landscape of Jiulong River watershed. The hydrologic and topographic parameters of AGNPS model were extracted based on DEM (Digital Elevation Model) built in GIS (Geographic Information System). The erosion and nutrients transport modules of AGNPS were tested and validated in Jiulong River watershed based on measurements and other data during the main crop growing season.教育部博士点基金 (19990 38411) ;; 福建省自然科学基金 (D0 110 0 0 3

金电极表面植物原生质体的固定化

Barley protoplast was immobilized on poly(dially dimethylammonium) (PDADMAC) modified gold electrode via the electrostatic attraction between the positively charged amine groups of PDADMAC and the negatively charged barley protoplast. STM image reveals that PDADMAC/Au(111) surface displays a nano organized dot(4-6 nm) array structure. The processes of the PDADMAC adsorption and of the barley protoplast immobilization were monitored from the in situ QCM frequency change. The effects of the adsorbed mass and of the density and viscosity of medium on the frequency change of QCM were discussed, respectively. The QCM result shows that the thickness of the adsorbed PDADMAC layer is 2.4 nm