An Analysis of CPPCC Member Role Performing Predicament and Countermeasure Suggestion: A Case Study Based on People’s Political Consultative of Quanzhou City

1949年9月21日，中国人民政治协商会议第一届全体会议召开以来，人民政协走过了63年多的光辉历程，它是我国的基本政治制度。团结和民主是人民政协的两大主题，政治协商、民主监督、参政议政是人民政协的三大职能。人民政协在建国以来的各个时期，都发挥着举足轻重的作用。政协委员是人民政协履行职能的主体，随着改革开放的不断深入和社会主义民主化进程的推进，政协委员在我国经济社会和各项事业中发挥着不可替代的主体作用。新形势下，人民政协在如何更好的发挥委员主体作用的探索研究过程中，积累了一定的经验，取得一显著的成效，但同时也存在一些问题，这些问题直接影响了政协委员主体作用的正常发挥，更不利于新形势下人民政协工作...Since its first plenary session on 21st Sept., 1949, Chinese People's Political Consultative Conference （CPPCC）, as China’s basic political system with unity and democracy as its two major themes, political consultation, democratic supervision, and participation in the administration and discussion of state affairs as its three major functions, has witnessed a glorious history of over 63 years. CP...学位：管理学硕士院系专业：公共事务学院_公共管理硕士(MPA)学号：X200713618

13-甲基十四烷酸对大鼠脑缺血后脑水肿的影响

目的研究13-甲基十四烷酸（13-methyltetradecanoic acid, 13-MTD）对脑缺血后脑水肿的影响及其机制。方法线栓法制备大鼠大脑中动脉闭塞模型,于插入栓线前30 min分别尾静脉注射13-MTD 40、80、120 mg·kg-1（M40、M80、M120）,阴性对照组给予等体积脂质体。于缺血6、12、24 h,以Longa神经功能缺失评分观察神经功能缺失症状;TTC染色观察脑梗死体积大小;AutoCAD图像分析软件计算脑水肿程度;脑干湿重测定脑含水量;伊文思蓝（EB）检测血脑屏障通透性;RT-PCR检测损伤侧脑组织AQP4 mRNA表达;免疫组化法检测损伤侧脑组织AQP4蛋白表达。结果 13-MTD可明显改善脑缺血大鼠神经功能缺失症状,使脑梗死体积明显缩小,使脑含水量和脑水肿程度明显减轻,EB渗出减少,明显改善缺血侧脑组织AQP4表达。结论 13-MTD能通过调控AQP4表达,减轻大鼠脑缺血后脑水肿。福建省教育厅中青年教师教育科研资助项目(No JA15861

Preparation and identification of polyclonal antibody raised against heat shock protein 90 of fission yeast

作者简介:李文珠(1983－),女,博士研究生,主要研究方向:细胞生物学 通信联系人：靳全文，教授，主要研究方向：细胞周期调控及表观遗传学， [email protected][中文文摘]为制备兔抗裂殖酵母Hsp90多克隆抗体,在通过PCR获得了裂殖酵母Hsp90(Swo1)基因后,构建了pMALc2x-Swo1表达载体,可用于表达编码正确氨基酸序列的目的基因。转化大肠杆菌BL21(DE3),IPTG诱导表达,Amylose Resin柱纯化。目的蛋白表达量占菌体总蛋白的30%以上。纯化后,蛋白纯度达95%以上。纯化后的MBP-Swo1融合蛋白抗原加福氏完全佐剂背部皮内注射首次免疫新西兰大白兔,第28天用MBP-Swo1融合抗原加福氏不完全佐剂同样剂量加强免疫,第35天时再次免疫。第49天心脏采血。收集血清后,用免疫印迹(westernblot)检测Swo1多克隆抗体的特异性。免疫印迹检测结果显示该抗体能够特异性识别内源性的裂殖酵母Hsp90/Swo1蛋白,但并不识别人类细胞中的Hsp90α/β。[英文文摘]To prepare the polyclonal antibody of Hsp90(Swo1) of fission yeast Schizosaccharomyces pombe,the encoding gene swo1+was amplified from the yeast genome and then inserted into expression vector pMALc2x.The resulted plasmid pMALc2x-Swo1 was transformed and expressed in E.coli BL21(DE3).The expressed fusion protein was purified through Amylose Resin column.The proteins were expressed mainly as secretion with the yield of more than 30% of total bacterial proteins.After purification,the purity of the proteins was about 95%. The New Zealand white rabbits were immunized with Freund’s complete adjuvant plus purified MBP-Swo1 fusion antigen through the back skin intradermal injection for the first time. The same dose of Freund’s incomplete adjuvant plus MBP-Swo1 was injected to strengthen the immunity after 28 and the 35 days respectively. After 49 days, blood sample was collected from heart, and antisera were extracted. The specificity of Swo1 polyclonal antibody was examined by Western blot. It showed that the antibody obtained had a high specificity to detect endogeneous Swo1 from fission yeast, but it could not recognize Hsp90α/β in human cells. Key words: fission yeast；Hsp90/Swo1；polyclonal antibody.高等学校博士学科点专项科研基金资助项目(20100121110003);国家自然科学基金资助项目(30871376);教育部科学技术研究重点项目(108076

罪犯改造成效评估量表的建构

本研究运用因素分析法，尝试建构我国监狱中罪犯改造成效评估量走。该量表分Ａ版（干警评定量表）与Ｂ版（服刑人员他评量表）。结果表明，该量表具有较高的信度、效度，所得的改造成效评估三因素结构合理，内容清晰</p

拟穴青蟹抗菌肽Crustin新变体的表达特性与抗菌功能

Crustins是一类较早发现并广泛分布在甲壳动物中且富含半胱氨酸的阳离子抗菌肽,能够参与抗细菌的免疫应答。从拟穴青蟹（Scylla paramamosain）中克隆获得Crustin基因,命名为SpCrus1a,其cDNA序列全长744 bp,开放阅读框编码113个氨基酸,成熟肽分子量10.03 kDa,等电点8.30。表达分析结果发现其转录本主要存在于鳃、卵巢、上皮组织中,脂多糖刺激后SpCrus1a会上调表达。体外合成SpCrus1a的第29～47位氨基酸进行抗菌活性实验发现,其对革兰氏阳性菌具有较强的抗菌活性,对被测的革兰氏阴性菌抗菌活性较弱或无抗菌活性。合成肽Sp Crus1a29-47能够在较短时间内杀死绝大多数的金黄色葡萄球菌,2 h内杀死全部的金黄色葡萄球菌。扫描电镜发现合成肽Sp Crus1a29-47可造成金黄色葡萄球菌表面结构崎岖不平,高浓度Sp Crus1a29-47造成细菌的大量死亡。上述结果表明,SpCrus1a是抗菌肽Crustins的新变体。国家自然科学基金(U205123,31172438);;厦门南方海洋研究中心科技项目(14PYY050SF03

Research on human visualization

人体可视化是指运用计算机图形学和图形处理技术,将抽象的人体数据转换为图形及图像在屏幕上显示出来并进行交互处理的理论、方法和技术。目前的许多应用已经显示出它在医学领域的巨大发展潜力。主要介绍人体可视化的研究背景、常用的可视化开发工具包、图像分割在医学可视化中的作用,最后对它的应用发展前景进行展望。Human visualization is a kind of theory,method and technology,which applies computer graphics and image processing technology to convert nonobjective human data to graphics and image to display on the screen and take mutual processing.The applications of human visualization reveal its great potential in medical field,In this paper,the research background of the human visualization in medical is introduced,the common use of visualization toolkits are summarized,the effect of image segmentation in the medical visualization is also concluded.Finally,the prospect of its applications in medical field is discussed

Polishing of Laser Lift-off-Induced GaN Surface

激光剥离(llO)技术是研制新型氮化镓(gAn)基谐振腔结构光电子器件的关键技术。然而llO后的gAn表面往往具有较大的粗糙度,而制作谐振腔结构器件需要很高的表面平整度,因此需要对llO后的gAn表面进行抛光。分别采用金刚石粉抛光液和胶粒二氧化硅抛光液进行机械抛光和化学机械抛光(CMP),并对比了两种方法获得的抛光结果,研究发现前者会在抛光后的gAn表面引入划痕,而采用后者可以得到亚纳米级平整度的表面。进一步的实验结果表明,胶粒二氧化硅抛光液同样适用于图形化衬底外延片激光剥离后的gAn表面抛光。Laser lift-off( LLO) is a key technology in development of new GaN-based resonant-cavity optoelectronic devices.For cavity-dependent devices,a smooth surface is highly required.However,the GaN surface after LLO is usually rough,and the polishing is necessary.The mechanical polishing of diamond powder and the chemical mechanical polishing of colloidal silica were compared.It is found that diamond powder leads to scratches on the GaN surface whereas colloidal silica leads to smooth surface with sub-nanometer roughness.The experiment results indicate that polishing with colloidal silica solutions can be applied to smoothen the LLO-produced GaN surface from an epitaxial wafer grown on patterned-sapphire substrate.国家自然科学基金资助项目(61274052;61106044); 中国科学院纳米器件与应用重点实验室开放课题资助项目(14ZS02

实验室建设与运行管理的探索与实践

文章阐述了高校实验室建设在\"双一流\"建设中的重要支撑作用。结合厦门大学化学化工学院物理化学实验室建设和管理的探索与实践,从物理化学实验室建设特点出发,探讨了如何统筹管理、规划、设计、整合、优化实验室布局结构等,并分享了实验室装修、改造过程中的思路和举措。建一流实验室促进实验教学的长远发展,充分发挥物理化学实验室在培养一流人才中的作用,以期推动\"双一流\"工程良好快速发展。2017年厦门大学教学改革研究项目(JG20170222);;\n国家基础科学人才培养基金项目(J1310024);;\n2016年度教育部“基础学科拔尖学生培养试验计划”研究课题资