Effect of steric hindrance on the biodegradation of dissolved methyl-phenanthrene

以菲(Phe)和甲基菲(MP:1-MP、3-MP、4-MP、9-MP)为研究对象,运用同步荧光法研究微生物Novosphingobium; pentaromativorans; US6-1对5种溶解态多环芳烃(PAHs)的降解特性.结果显示:MP在8*10~(-7),1.2*10~(-6)mol/L2种浓度下,其生物降解; 过程符合零级动力学,降解速率均为:4-MP<9-MP<1-MP<3-MP;5种PAHs的最大生物降解速率同理论最大跨膜通量存在良好的线性关系;N; aN_3对Phe降解速率影响较小,但抑制MP降解且抑制程度与取代基位置有关.The biodegradation of dissolved phenanthrene (Phe) and; methyl-phenanthrene (MP), including 1-MP, 3-MP, 4-MP and 9-MP by the; bacterium Novosphingobium pentaromativorans US6-1was individually; studied using synchronous fluorescence spectrometry. The biodegradation; of MP was adequately described by zero-order kinetics at two; concentrations, i.e., 8*10~(-7)mol/L and 1.2*10~(-6)mol/L. The rates of; biodegradation were generally in the following order:; 4-MP<9-MP<1-MP<3-MP. There was a good linear relationship between the; measured maximum biodegradation rates and the theoretical maximum; transmembrane fluxes. The addition of NaN_3exerted little effects on the; biodegradation of Phe but inhibited that of MP, with the degree of; inhibition dependent on the position of the methyl substituent.国家自然科学基金资助项目; 国家海洋局海洋溢油鉴别与损害评估技术重点实验室开放基金资助项目; 高等学校博士学科点专项科研基金资助项

溶解态菲及其中间代谢产物1-羟基-2-萘甲酸的同时测定

运用导数-同步荧光光谱建立了快速同时测定溶解态菲(Phenanthrene,Phe)及其中间代谢产物1-羟基-2-萘甲酸(1-Hydroxy-2-naphthoic acid,1H2NA)的分析方法。所建方法同时测定Phe与1H2NA的线性范围分别为4.0~1.0×103μg/L和4.0~1.2×103μg/L,检出限分别为0.08μg/L和0.07μg/L,加标回收率分别为96.5%~105.3%和99.2%~106.5%,相对标准偏差均小于1.0%。该方法可应用于实验室条件下溶解态Phe微生物降解过程中Phe和1H2NA的测定,具备原位研究Phe降解过程中间代谢产物1H2NA的应用潜力。国家自然科学基金资助项目(21577110,21627814

一种用于分子发光分析的外加磁场装置

本文介绍了一种自行设计的能将外加磁场效应引入现有分子发光分析仪器的外加磁场装置.讨论了装置的组成、特点和在分子发光分析法中的实际应用以及该装置在研究生命科学、生物体系磁效应等方面的潜在意义

Effects of External Magnetic Field on Environmental Behavior of Some Organic Pollutants

[中文文摘]讨论了用荧光偏振法、荧光法和室温磷光法（ ＲＴＰ） 研究外加磁场效应（ ＭＦＥ） 对多环芳烃（ ＰＡＨｓ） 与溶解态有机物（ 如腐植酸） 间相互作用的影响， 以及ＭＦＥ 对生物降解ＰＡＨｓ 的影响的２种方法．[英文文摘]Two molecular luminescence methods have been developed for studying the effects of external magnetic field on the environmental behavior of some organic pollutants.One of them is using fluorescence polarization method to study the effects of external magnetic field on the interaction of organic pollutants with dissolved organic materials.And the other is using fluorescence and RTP method to study the effects of external magnetic field on biodegradation of some polynuclear aromatic hydrocarbons(PAHs) in water.国家自然科学基金; 福建省自然科学基金（D9910008）

Room-temperature Phosphorescence of Carbaryl Induced byβ-Cyclodextrin in the Presence of Cyclohexane

[中文文摘]将环己烷(CH)引入β_环糊精(β_CD)诱导西维因(CBL)室温磷光(RTP)体系,由于CH和β_CD及CBL形成包络物,可观测到CBL强的RTP。研究了温度、pH值以及形成包络物的3种组分物质的浓度变化对这一体系RTP的影响,优化了实验条件,建立了CBL的RTP测定方法。实验结果表明,在2.0~20.0μg/L质量浓度范围内,CBL的RTP强度和其浓度成正比,方法检出限达0.43μg/L,相对标准偏差小于1.50%(n=7)。将该方法用于合成样品的分析,结果满意。[英文文摘]An intensive room-temperature phosphorescence(RTP)of Carbaryl(CBL)was observed inβ-Cy-clodextrin(β-CD)aqueous solution in the presence of Cyclohexane(CH)due to the formation ofβ-CD/CBL/CHinclusion complexes.The effects oftemperature,pHvalues and the variation of concentrations ofeach com-ponent on RTP have been investigated.Under the optimal conditions,an analytical method for the determina-tion of CBL in synthetic samples was established.The correlation coefficient of the calibration curve of CBL was0.9943 and the linear range was of 2. 0 - 20. 0μg/ L. The detection limit was 0. 43μg/ L and the average relative standard deviation was less than 1.50%(n=7) .国家自然科学基金资助项目(20377035

Sorption /desorption behaviors of pyrene on mangrove sediment aggregates with different sizes

采用海藻酸钙凝胶包覆的方法,研究了芘在九龙江河口红树林区不同粒径沉积物团聚体上的吸附/解吸行为.结果表明,海藻酸钙凝胶包覆可有效减少沉积物团聚体粒径的改变,其等温吸附、吸附/解吸速率情况与未包覆沉积物团聚体存在明显差异.包覆后等温吸附曲线中吸附容量kf值取决于总有机碳(TOC)的含量,非线性吸附常数n不仅与其硬碳和软碳的比值有关,还与团聚体复杂的孔隙结构存在着一定的关系.包覆后沉积物团聚体的快吸附速率常数值与沉积物团聚体的比表面积值正相关(krAP=0.041S-0.0146,r2=0.9923).小粒径沉积物团聚体的“老化“现象较大粒径更为显著,使得其解吸量较小.The sorption behaviors of pyrene( Pyr) to mangrove sediment aggregates treated with the enclosure of calcium alginate beads were investigated.Experimental results showed that the stabilization of mangrove sediment aggregates with different particle sizes was increased effectively with the presence of calcium alginate beads.Great difference existed in the sorption capacity for Pyr between the sediment aggregate with / without the enclosure of calcium alginate beads,which was also the cose for the sorption / desorption kinetic constant.The sorption capacity for Pyr was closely associated with the content of total organic carbon in mangrove sediment aggregates,and the nonlinear sorption constant was related to the ratio of carbonaceous to amorphous organic matter,and the complexity structure of mangrove sediment aggregate.Additionally,the rapid sorption kinetic rates were found to exhibit positive correlation with specific surface area of mangrove sediment aggregate( krap= 0.041S- 0.0146,R2= 0.9923),and the desorption capacity of Pyr in smaller aggregate was less than that in the larger ones,which can be explained by the higher aging degrees of smaller aggregate.国家自然科学基金(21075102;21177102;41121091); 教育部博士点基金(200803840015)资

动力学室温磷光法测定α-溴代萘

报道用动力学室温磷光法测定α－溴代萘（α－ＢｒＮｐ）的新方法．检出限为３．５８×１０－５ｍｏｌ／Ｌ，相对标准偏差小于１６．２％

荧光偏振法测定苝与富勒酸的结合常数

建立了用荧光偏振法研究苝和富勒酸之间的相互作用的方法． 结果表明， 苝和富勒酸之间的相互作用强烈． 本方法的一个优点是不经分离既可测定荧光物的结合常数

动力学室温磷光法的初步研究

室温磷光（ＲＴＰ）法作为一种新的测定痕量有机物和生化物质的高灵敏度和高选择性的测试手段，在生命科学、环境科学等方面有着广阔的应用前景．近十年来，该方法在我国倍受关注，得到迅速发展［１］．然而，动力学ＲＴＰ法不论是作为分析方法，还是作为研究手段，国内外..

同步荧光法同时测定苯甲酸和水杨酸的研究

根据苯甲酸与水杨酸在酸性条件下荧光光谱出现的差异，用同步荧光法同时测定其混合物中二组份。苯甲酸及水杨酸的检测限分别为０．２４μｇ／ｍＬ和０．２５μｇ／ｍＬ，相对标准偏差分别为１．３％和１．２％。方法用于商品杀菌防腐剂中苯甲酸与水杨酸的同时测定，结果满意。Ａｂｓｔｒａｃｔ：Ｂａｓｅｄｏｎｔｈｅｅｖｉｄｅｎｔｄｉｆｆｅｒｅｎｃｅ，ｏｆｆｌｕｏｒｅｓｅｅｎｃｅｓｐｅｔｒａｃｈａｒａｃｔｅｒｉｓｔｉｃｓｂｅｔｗｅｅｎｂｅｎｚｏｉｃａｃｉｄａｎｄｓａｌｉｃｙｌｉｃａｃｉｄｉｎ１．０ｍｏｌ／ＬａｑｕｅｏｕｓＨ２ＳＯ４ｓｏｌｕｔｉｏｎ，ａｎｅｗｍｅｔｈｏｄｈａｓｂｅｅｎｅｓｔａｂｌｉｓｈｅｄｆｏｒｓｉｍｕｌｔａｎｅｏｕｓｄｅｔｅｒｍｉｎａｔｉｏｎｏｆｂｅｎｚｏｉｃａｃｉｄａｎｄｓａｌｉｃｙｌｉｃａｃｉｄｉｎｍｉｘｔｕｒｅｓｂｙｓｙｎｃｈｒｏｎｏｕｓｆｌｏｕｒｉｍｅｔｒｙ．Ｔｈｅｄｅｔｅｃｔｉｏｎｌｉｍｉｔｓｏｆｂｅｎｚｏｉｃａｃｉｄａｎｄｓａｌｉｃｙｌｉｃａｃｉｄａｒｅ：０．２４μｇ／ｍＬａｎｄ０．２５μｇ／ｍＬ，ｒｅｓｐｅｃｔｉｖｅｌｙ．Ｔｈｅｒｅｌａｔｉｖｅｓｔａｎｄａｒｄｄｅｖｉ