基于GEO数据库发现促前列腺癌转移基因SERPINE1及其临床意义

目的分析上皮间质转换(EMT)相关基因在前列腺癌肿瘤细胞转移前后的变化,并筛选有潜在研究价值的基因,研究其临床价值,并初步探索其可能的分子机制。方法通过EMT通路的聚合酶链式反应(PCR)芯片分析两种具有不同转移能力的前列腺癌细胞表达差异,并结合GSE16560、Kaplan-Meier、Oncomine及基因探针富集分析(GSEA)数据库筛选潜在研究对象及相关通路。结果丝氨酸蛋白酶抑制蛋白E1(SERPINE1)表达与前列腺癌中较高的Gleason评分正相关(6分vs.7分,P=0.003 2,6分vs.≥9分,P=0.001 3),转移组的SERPINE1表达高于未转移组(P=0.028 3),SERPINE1低表达组生存期长于高表达组(P=0.024 5)。Oncomine数据库显示在多种肿瘤中,152项肿瘤独立分析中SERPINE1高表达,相对应的是61项正常组织独立分析中SERPINE1低表达。Kaplan-Meier曲线分析也表明,SERPINE1在肺癌(P=0.04)、胃癌(P=0.017)、卵巢癌(P=0.009 1)中均表现为不良的预后因素。GSEA结果表明高表达SERPINE1与血管生成等过程有相关性。结论高表达SERPINE1的前列腺癌患者Gleason评分也相对较高,转移的可能性大且预后较差,同时SERPINE1可能通过影响血管生成等过程促进前列腺癌转移。国家自然科学基金(No.81572529

Development of a Conduct Problem Tendency Inventory for Adolescents

目的：探讨我国青少年学生的品行问题行为倾向并编制相应的测量工具。方法：首先采用自下而上的思路进行深度访谈,探索在我国青少年学生中多发的品行问题行为并形成试测问卷;然后经小样本试测,运用探索性因素分析提取三个因素,形成修改问卷;最后对1923名青少年学生施测,使用验证性因素分析修正因素结构,形成正式问卷。结果：青少年学生中多发的问题行为包括违规行为、成瘾行为和攻击行为,分别对应问卷中的违规倾向、成瘾倾向和攻击倾向;量表的结构方程模型拟合良好（χ^2=316.447,df=74,χ^2/df=4.276,GFI=0.974,AGFI=0.964,RMSEA=0.042）,各项信效度指标良好（内部一致性系数α=0.797）。结论：青少年品行问题行为倾向问卷具有良好的信效度

JUNO Sensitivity on Proton Decay p→νˉK+p\to \bar\nu K^+ Searches

The Jiangmen Underground Neutrino Observatory (JUNO) is a large liquid scintillator detector designed to explore many topics in fundamental physics. In this paper, the potential on searching for proton decay in $p\to \bar\nu K^+$ mode with JUNO is investigated.The kaon and its decay particles feature a clear three-fold coincidence signature that results in a high efficiency for identification. Moreover, the excellent energy resolution of JUNO permits to suppress the sizable background caused by other delayed signals. Based on these advantages, the detection efficiency for the proton decay via $p\to \bar\nu K^+$ is 36.9% with a background level of 0.2 events after 10 years of data taking. The estimated sensitivity based on 200 kton-years exposure is $9.6 \times 10^{33}$ years, competitive with the current best limits on the proton lifetime in this channel

JUNO sensitivity on proton decay p → ν K + searches*

The Jiangmen Underground Neutrino Observatory (JUNO) is a large liquid scintillator detector designed to explore many topics in fundamental physics. In this study, the potential of searching for proton decay in the $p\to \bar{\nu} K^+$ mode with JUNO is investigated. The kaon and its decay particles feature a clear three-fold coincidence signature that results in a high efficiency for identification. Moreover, the excellent energy resolution of JUNO permits suppression of the sizable background caused by other delayed signals. Based on these advantages, the detection efficiency for the proton decay via $p\to \bar{\nu} K^+$ is 36.9% ± 4.9% with a background level of $0.2\pm 0.05({\rm syst})\pm$$0.2({\rm stat})$ events after 10 years of data collection. The estimated sensitivity based on 200 kton-years of exposure is $9.6 \times 10^{33}$ years, which is competitive with the current best limits on the proton lifetime in this channel and complements the use of different detection technologies