The Mechanism on the Regulation of Jacaranone in cIAP2-mediated TNFα Survival and Death Conversion

凋亡抑制蛋白IAPs调控细胞的多种生物学进程，其过表达与肿瘤生长、不良预后及疗效相关，在大多数人类恶性肿瘤中表达异常升高。cIAPs参与细胞命运的调控过程。TNFα结合TNFR1引起TRADD、TRAF2、TRAF5、cIAP1、cIAP2以及蛋白激酶RIP1的募集形成复合物I。cIAPs介导复合物I组分的泛素化，如RIP1，进而诱导IKK复合物的募集。IKKβ磷酸化NF-κB抑制子IκB，使其靶向泛素化依赖的蛋白酶体降解，从而使NF-κB转移至细胞核启动靶基因的表达。在缺乏cIAPs的情况下，RIP1从膜结合的复合物I上解离，与FADD、caspase8形成复合物II，导致caspase8的...Apoptosis inhibitory protein IAPs regulate a variety of biological processes. cIAPs are abnormally elevated in most human malignancies, and their overexpression is associated with tumor growth, poor prognosis and efficacy. cIAPs are involved in the regulation of cell fate. TNFα binds TNFR1 to induce TRADD, TRAF2, TRAF5, cIAP1, cIAP2 and protein kinase RIP1 to form complex I. cIAPs mediate the ubiq...学位：医学硕士院系专业：药学院_药理学学号：3232014115343

Chemotaxis and characteristics of chemotactic genes in Novosphingobium strains

【目的】选择了几株隶属于新鞘氨醇杆菌属(Novosphingobium)的有和没有多环芳烃(PAHs)降解能力的细菌,在基因组水平比较分析它们的; 趋化通路,并探究其中一些菌株对芳香化合物和三羧酸(TCA)循环中间代谢产物的趋化性。【方法】通过基因组的比较分析,研究了几株新鞘氨醇杆菌的趋化蛋; 白组成和趋化基因分布,并采用滴定法和游动平板法检测了相关菌对芳香族化合物和TCA循环中间代谢产物的趋化性。【结果】N.; pentaromativorans US6-1、N. pentaromativorans F2、Novosphingobium indicum; K13、 Novosphingobium stygium DSM 12445、Novosphingobium sp.; C2AC等5株新鞘氨醇杆菌对芳香族化合物和TCA循环中间代谢物具有不同程度的趋化性;所选的7株已完成基因组测序的新鞘氨醇杆菌N.; pentaromativorans F2、N. pentaromativorans US6-1、Novosphingobium sp.; PP1Y、Novosphingobium sp. AP12、Novosphingobium sp. Rr; 2-17,Novosphingobium sp. B-7和Novosphingobium sp. DSM; 19370均含有趋化蛋白MCP、CheW、CheA、CheB、CheR和CheY,且亲缘关系最近的US6-1、F2和PP1Y; 3株菌的che基因簇中的基因排列一致,为cheW-Y-D-B-R-A-(X);新鞘氨醇杆菌的趋化系统属Fla类型。; 【结论】几株新鞘氨醇杆菌都具有较为完整的趋化通路,且对多种芳烃及其代谢物的趋化性各不相同,其中菌株US6-1趋化现象最明显。[Objective] The present study aims to analyze the chemotaxis genes and; proteins of several PAH-degrading Novosphingobium strains, and the; chemotaxis of these strains toward aromatic compounds and intermediates.; [Methods] Based on genome comparative analysis, we identified the; chemotaxis genes organization and proteins distribution. We used drop; and swarm plate assays to detect the chemotaxis of these strains toward; aromatic compounds and intermediates of TCA cycle. [Results] We found; that all these Novosphingobium strains showed chemotaxis, but the; chemotatic ability varied. The completed genome sequenced strains N.; pentaromativorans F2, N. pentaromativorans US6-1, N. pentaromativorans; PP1Y, Novosphingobium sp. AP12, Novosphingobium sp. Rr 2-17, and; Novosphingobium nitrogenifigens DSM 19370 contained MCP, CheW, CheA,; CheB, CheR and CheY. Strain F2, US6-1 and PP1Y, shared a consistent; order of chemotaxis genes in che cluster. The chemotatic system of these; Novosphingobium strains belonged to the Fla chemotactic system.; [Conclusion] These strains all contained a complete chmotaxis pathway.; Their chemotactic ability toward aromatic compounds and intermediates; varied, and the chemotaxis of US6-1 was obvious.国家自然科学基金; 福建省自然科学基

Assessment of genetic diversity in Amitostigma pinguiculum revealed with SRAP Markers(大花无柱兰遗传多样性的SRAP 标记分析)

大花无柱兰是一种珍稀兰科植物，具有一定的观赏和药用价值，但数量十分稀少，该物种亟待保护。本研究采用SRAP 分子标记技术，对10 个居群的115 份DNA 样品进行PCR 扩增，并开展遗传多样性分析。从81 对引物中筛选出9 个条带清晰、多态性好、重复性高的引物组合，共扩增得到305 条谱带。在物种水平上，多态性比率（PPB） 为100%，Nei’s 基因多样性指数（H） 为0.209 8，Shannon’s 指数（I） 为0.340 2；在居群水平上，PPB 为24.59%～52.13%，H 为0.079 6～0.165 5，I 为0.120 9～0.252 3。居群水平上，基因分化度（Gst）为0.520 9，基因流（Nm） 为0.459 9，遗传距离为0.091 9～0.198 4。UPGMA 聚类结果表明，10 个居群可分为3大类，地理距离相近的居群优先聚集。大花无柱兰的遗传多样性较为丰富，居群间存在一定的遗传分化和基因交流，可采用就地保护和人工栽培等方式加以保护

JUNO Sensitivity on Proton Decay p→νˉK+p\to \bar\nu K^+ Searches

The Jiangmen Underground Neutrino Observatory (JUNO) is a large liquid scintillator detector designed to explore many topics in fundamental physics. In this paper, the potential on searching for proton decay in $p\to \bar\nu K^+$ mode with JUNO is investigated.The kaon and its decay particles feature a clear three-fold coincidence signature that results in a high efficiency for identification. Moreover, the excellent energy resolution of JUNO permits to suppress the sizable background caused by other delayed signals. Based on these advantages, the detection efficiency for the proton decay via $p\to \bar\nu K^+$ is 36.9% with a background level of 0.2 events after 10 years of data taking. The estimated sensitivity based on 200 kton-years exposure is $9.6 \times 10^{33}$ years, competitive with the current best limits on the proton lifetime in this channel

JUNO sensitivity on proton decay p → ν K + searches*

The Jiangmen Underground Neutrino Observatory (JUNO) is a large liquid scintillator detector designed to explore many topics in fundamental physics. In this study, the potential of searching for proton decay in the $p\to \bar{\nu} K^+$ mode with JUNO is investigated. The kaon and its decay particles feature a clear three-fold coincidence signature that results in a high efficiency for identification. Moreover, the excellent energy resolution of JUNO permits suppression of the sizable background caused by other delayed signals. Based on these advantages, the detection efficiency for the proton decay via $p\to \bar{\nu} K^+$ is 36.9% ± 4.9% with a background level of $0.2\pm 0.05({\rm syst})\pm$$0.2({\rm stat})$ events after 10 years of data collection. The estimated sensitivity based on 200 kton-years of exposure is $9.6 \times 10^{33}$ years, which is competitive with the current best limits on the proton lifetime in this channel and complements the use of different detection technologies