Usefulness of mycobacterium tuberculosis-specific interferon-γ release assay in investigating the background value of it in healthy elderly population in the region

目的:探索本地区45岁以上健康中老年人群血浆结核分支杆菌特异性γ-干扰素本底值,为Tb-IgrA定量试验广泛应用于结核病的临床诊断、疗效观察提供本底值参照依据。方法:选取100位45岁以上健康中老年人,结核分枝杆菌(MTb)γ-干扰素体外释放定量试验测定其血浆中特异性γ-干扰素含量。结果:盐城市100位健康中老年人血浆特异性γ-干扰素含量为0.1 Pg/Ml~7.1 Pg/Ml,中位数为1.1 Pg/Ml。结论:本地区健康中老年血浆中特异性γ干扰素含量存在一定的本底值,在使用Tb-IgrA定量试验方法进行结核病临床诊断、疗效观察时要充分考虑健康人群本底值带来的影响。Objective: To explore the background value of Mycobacterium Tuberculosis-specific Interferon-γ in healthy population with age over 45 years in Yancheng city and to provide reference for TB-IGRA test widely used in clinical diagnosis of TB and efficacy observation.Methods: Mycobacterium tuberculosis(MTB) interferon-γ test in vitro quantitative form was used to detect the specific interferon-α level in plasma of 100 selected healthy elderly over 45 years.Results: Plasma concentration of interferon-γ in 100 selected elderly was 0.1 pg/ml--7.1 pg/ml,with median of 1.1 pg/ml.Conclusion: There is a certain background value of MTB-specific interferon-γ in plasma of the elder over 45 years in the region,and the impact of which should be given full consideration when using the TB-IGRA quantitative test methods for clinical diagnosis of tuberculosis and efficacy observation

产胞外布雷菲德菌素A青霉发酵条件的研究

通过正交设计试验,优化了青霉(Penicillium)属真菌SHZK-15产胞外布雷菲德菌素(B refeld in-A,简称BFA)的液体发酵条件。最佳的BFA发酵条件为:培养基含马铃薯200g(煮汁),葡萄糖20g,(NH4)2SO44.0g,KH2PO41.0g,MgSO4.7H2O 2.0g,CaCO35.0g,pH自然,瓶装量为100mL/250 mL,培养温度为28℃,转速为120 r/m in,培养时间为7d,BFA的最高产量可达151.6mg/L

研究生对生成性课堂的参与(笔谈)——厦门大学教育研究院“高等教育研究方法”第十三次课反思

\"高等教育研究方法\"这门课具有研究型课堂的特征。其第十三次课的主题是对反思日志做一次元研究。反思日志是学习\"高等教育研究方法\"课的重要工具,它不仅是课堂教学的缩影,而且是课堂时效的呈现,更是学生自我成长的印证。反思日志勾勒着整个课堂进程的图景,反映着学生的收获、困惑、不足以及对自己未来改进方向的认识等。第十三次课以生成式教学引导学生主动参与课堂,通过对反思日志重新审视,让学生成为思考的主体、学习的主体、研究的主体,实现自我成长、自我发现、自我唤醒的课堂教学改革目标

再酯化甘油三酯型鱼油中不同结构酯的 含量及稳定性分析

再酯化甘油三酯（rTG）型鱼油是甘油三酯(TG)、甘油二酯(DG)、甘油一酯(MG)和乙酯(EE)的混合物。为了解rTG型鱼油不同结构酯的组成及其对产品稳定性的影响，采用高效液相色谱-示差折光检测器检测了来自国内外10个厂家的18个批次rTG型鱼油中不同结构油脂的含量，与天然TG型鱼油和EE型鱼油进行比较，并考察了TG含量对rTG型鱼油产品稳定性的影响。结果表明：rTG型鱼油中TG含量在52.17%~94.80%，其中只有4个批次样品高于90%，偏甘酯含量在4.96%~38.97%，EE含量在0.28%~13.10%，其中有7个批次样品EE含量超过了5%，不符合欧洲药典（EP10.3）要求；天然TG型鱼油的TG含量在97%以上，EE型鱼油的EE含量在99%以上；与TG含量低( 64.09%）的rTG型鱼油产品相比，TG含量高（90.80%）的rTG型鱼油产品酸值和过氧化值相对原料变化较小；加速氧化过程中，TG含量高的rTG型鱼油产品的酸值稳定,但TG含量低的rTG型鱼油产品酸值明显增高。rTG型鱼油产品的稳定性可能与TG含量有关，TG含量高的rTG型鱼油的稳定性比TG含量低的更好。Abstract：Re-esterified triglyceride (rTG) type fish oil is a mixture of triglycerides (TG), diglycerides (DG), monoglycerides (MG) and ethyl esters (EE). In order to learn about the distribution of different structural esters in rTG type fish oil and their effects on product stability, 18 batches of rTG type fish oil from 10 factories in domestic and abroad were collected, the contents of different structure esters were determined by HPLC-differential refractive index detector and compared to nature TG type fish oil and EE type fish oil, and effect of TG content on the stability of fish oil product was investigated. The results showed that the TG content in rTG type fish oil was 52.17%-94.80%, among which only 4 batches samples were higher than 90%. The content of partial glycerides ranged from 4.96% to 38.97%, and the EE content ranged from 028% to 13.10%, with 7 batches samples exceeding 5%, which did not meet the requirements of European Pharmacopoeia(EP10.3). The TG content in natural TG type fish oil was above 97%, and the EE content in EE type fish oil was above 99%. Compared with rTG type fish oil products with low TG content (64.09%), rTG type fish oil products with high TG content (90.80%) had relatively small changes in acid value and peroxide value compared to raw materials. During the accelerated oxidation process, the acid value of rTG type fish oil products with high TG content was stable, while the acid value of rTG type fish oil products with low TG content significantly increased. TG content may be related to the stability of rTG type fish oil, and rTG type fish oil with high TG content has better stability than rTG type fish oil with low TG content

The effect of phosphoryl oxygen on the intermolecular action of alanine and lysozyme

It was found that N-diisopropyloxyphosphoryl alanine (DIPP-Ala) could form adduct with hen egg white lysozyme (HEWL) as shown in electrospray ionization mass spectroscopy (ESI-MS), but the non-phosphorylated alanine couldn't. The capability for formation of DIPP-Ala dimmer was more stronger than that of alanine. It suggested that a specific non-covalent complex was formed in the solution phase and could be transferred to the gas phase via electrospray ionization (ESI). The results implied that phosphorylated alanine possessed relatively stronger affinities for protein and formed non-covalent complexes with protein more easily than alanine. Using Tripos force field, molecular mechanics calculation on DIPP-Ala dimmer showed that such non-covalent adduct formation was due to the intermolecular hydrogen bond

浅谈实施初中化学生活化教学对策

生活化教学方式在提高学生学习化学兴趣的同时，还发挥着促进学生主观能动性形成的作用，而在传统课堂中管理方式的愈加科学、传授内容的愈加精巧、教学手段的愈加先进，其不能在一定程度上激发起学生学习的欲望和积极性。因此，教学回归生活化的任务已经成为教师教学过程中亟须解决的关键性问题。</jats:p

新课改背景下的小学数学高效课堂构建探究

在当前素质教育广泛开展和新课改深入推进的教育形势下，教师的教学活动的有效性已经成为评价教师教学水平和教学质量的重要标准之一，因此，教师亟需对自身的教学方式方法进行改进和创新，结合新课标要求，设计科学合理的教学计划，实现教学活动的有序推进。本文笔者结合当前的教育理论与数学教学现状，深入分析小学数学有效性的具体措施，简要发表自己的见解，旨在为广大教育工作者在完善自身教学方式的过程中提供一定的思路和参考。</jats:p

环面渐开线齿轮的动态接触分析

以环面渐开线齿轮为研究对象,根据齿轮啮合原理及滚齿加工原理,利用产形齿条的齿面方程,分别推导出凸、凹环面渐开线齿轮齿面方程。根据不产生尖化或根切的数学条件,基于MATLAB开发了环面渐开线齿轮几何参数设计的Graphical User Interface。以环面渐开线齿轮齿面上点的三维坐标构建三维实体模型,并将实体模型导入Hypermesh中划分网格。基于非线性接触算法,建立凸—凹环面渐开线齿轮啮合的有限元模型,在ABAQUS中对其进行动态接触分析,结果表明环面渐开线齿轮齿宽受根切及尖化的限制,而在齿面接触应力方面,双齿啮合区齿面接触应力较小,单齿啮合区齿面接触应力较大

Apriori-based Web Traversal Pattern Mining Algorithm

简要介绍Apriori算法与Web访问路径的有向图表示方法,提出一种从Web日志文件中得到频繁访问模式的算法,该算法以Apriori算法为基础,并以访问路径序列的有序性特点作为候选集的剪枝策略,减少候选集的生成,提高算法的效率。在真实数据集和模拟数据集上分别进行实验,实验结果表明该算法是有效的,且适应性好。The Apriori algorithm and the directed graph representation method for Web traversal paths are briefly introduced, and an algorithm based on Apriori is proposed for generating frequent traversal patterns from Web log files. The proposed algorithm u- ses the orderliness of the traversal paths as pruning strategy of candidate set, thus it can decrease the scale of candidate sets and improve efficiency. Some experiments are conducted with real datasets and simulated datasets, and the experimental results show the effectiveness and good adaptability of the proposed algorithm.科学计算与智能信息处理广西高校重点实验室基金项目(GXSCIIP201201);广西教育厅科研项

衰老、慢性应激与免疫细胞热休克蛋白70反应抑制

自然衰老和慢性应激都能导致免疫细胞对环境刺激的抵抗力降低。衰老和慢性应激影响免疫细胞功能的分子机制目前还不清楚。热休克蛋白（HSP）是一类在所有原核细胞和真核细胞中均有表达的高度保守的蛋白质。研究表明，HSP反应是细胞维持正常功能，对抗应激性损伤的基本保护性机制之一。在HSP家族中，热休克蛋白70（HSP70）对应激条件最敏感，且被认为是功能最重要的一类热休克蛋白