Preparation,characterization,and application of monoclonal antibodies against human decoy receptor 3

目的制备抗DcR3单克隆抗体(mAb),鉴定其生物学特性,并应用于ELISA、Western blot、Flowcytometry(FCM)检测。方法以纯化的可溶性DcR3(sDcR3)免疫Balb/c小鼠,采用杂交瘤技术制备抗DcR3 mAb。用Ig亚类ELISA试剂盒鉴定抗DcR3mAb的亚类。用ELISA方法测定抗DcR3 mAb与sDcR3结合的特性,SDS-PAGE鉴定抗DcR3 mAb与SW480细胞上清中DcR3结合的特性,以鉴定mAb的特性。用间接ELISA法检测腹水mAb的效价、亲和常数并进行表位分析。Western blot检测mAb的特异性及应用,并用所获抗DcR3单克隆抗体(mAb)通过流式细胞仪检测肿瘤细胞表面DcR3的表达水平。结果获得4株可分泌DcR3 mAb的杂交瘤细胞系ZZ-393、ZZ-394、ZZ-151和ZZ-268。其中DcR3 mAb ZZ-268(下文简称为ZZ-268)的Ig亚类为IgG1(κ型);腹水效价为1×10-5;亲和常数为1.28×109水平;ZZ-268和ZZ-151可识别与其他2种抗体不同的抗原表位;Western blot证实,获得的ZZ-268... 【英文摘要】 Objective To prepare monoclonal antibodies mAb against DcR3,characterize their biological properties and apply them to ELISA,Flow cytometry,and Western blot detection.Methods Balb/c mice were immunized with soluble DcR3(sDcR3) for preparing mAb by using hybridoma technique.Ig subclass and specificity of mAbs were analyzed by ELISA and SDS-PAGE,respectively.The titres,relative affinity,and epitopes of mAbs in ascitic fluid were determined by indirect ELISA.The specificity and application of mAb were detected...厦门大学科研启动资金(Z03103);; 厦门市科技计划项目(3502Z20083008)资

活心丸(浓缩丸)治疗冠心病稳定性心绞痛的多中心、随机、双盲、安慰剂对照临床研究

目的观察活心丸(浓缩丸)治疗冠心病稳定性心绞痛的临床疗效和安全性。方法本研究采用多中心、随机、双盲、安慰剂对照研究方法,将480例气虚血瘀型冠心病稳定性心绞痛患者以3:1比例随机分为试验组(360例)和对照组(120例)。在西医常规治疗基础上,两组患者分别服用活心丸(浓缩丸)和安慰剂,1次2粒,每日3次;疗程8周。观察两组治疗前后主要指标(心绞痛症状积分)和次要指标(包括硝酸甘油减停率、中医证候积分及生活质量评价)及安全性。结果共454例患者完成试验(试验组336例,对照组118例)。与本组治疗前比较,试验组和对照组心绞痛症状积分和中医证候积分均降低,西雅图心绞痛量表评分改善(P<0.01);与对照组治疗后比较,试验组心绞痛症状积分、中医证候积分、西雅图心绞痛量表评分改善均优于对照组(P<0.01)。试验组心绞痛症状疗效总有效率为80.95%,硝酸甘油停减率为80.70%,中医证候疗效有效率80.65%,均高于对照组[36.44%、45.07%、38.99%(χ~2=58.21、40.94、66.55,P<0.01)]。试验组中既往有心梗史患者心绞痛症状总有效率为83.22%,中医证候有效率83.22%,西雅图心绞痛量表评分为(361.74±62.10)分,均高于无心梗史患者[60.92%、66.89%、(327.95±65.07)分(χ~2=13.89、13.26,P<0.01)]。治疗过程中未发生明显不良反应。结论活心丸(浓缩丸)治疗气虚血瘀型冠心病稳定性心绞痛疗效显著,尤其适用于既往有心梗病史的冠心病稳定性心绞痛患者,无明显不良反应。国家中医药管理局资助课题(No.国中医药科2016ZX04

XML语义问题及其处理

XML是一种承诺创建定制的标记集合以对特定类型信息编码的元语言。它不是一种具体的解决方案，而是一个用来设计标记的schema。XML关注内容，它使数据和数据的表示形式分开。用XML可以设计各种各样的标记语言。不同用途的标记语言，对显示的要求也不同。目前所谓的XML浏览器仅仅允许用户浏览XML标记的结构，而不提供XML实际意义的显示。要以有意义的方式浏览XML数据，必须研究标记语言的语义，有针对性地解决XML的显示问题。目前，数学信息在Web上的交流主要通过静态的图片方式，这种方法有很多缺陷：数学信息的显示不能与周围环境保持一致，并且不能包含数学信息的内容以及语义。W3c制订的数学标记语言（MathML）是一种能够同时描述数学符号以及数学信息的结构语义的XML应用，它能够很好地完成交流数学信息的任务。本文提供了一个数学标记语言处理器的具体设计。这个处理器结合 MathML的语义，解决了在浏览器中显示数学信息的问题。同时，它能够解析MathML文档，并向用户提供一个简便地生成MathML表述标记文档的接口。本文提供的设计思想对于实现通用的XML浏览器有一定的意义。XML is a meta language which can establish sets of markup to encode particular information. It is not a concrete language, but a schema to design tags. XML concerns content, and distinguishes data from their presentations. There are many markup languages designed by XML. Depending on different purposes, displays of markup languages will differ . Nowadays, We can only get the structure of XML tags, but can not browse the meaning of markup languages through XML browsers. To browse the significance of XML, We must investigate the semantics of XML, and solve the display problems. At present, most mathematics on the Web is GIF or JPEG format, which is difficult to consist with the context and can not include contents and semantics information of the mathematics. Mathematical Markup Language is one of XML applications which can encode both notions and contents of mathematics. It is an excellent tool to exchange mathematics on the Web. In this paper, We provide the design details of a MathML processor which solves mathematics display problems in a browser. The processor can also parser MathML documents and generate MathML present element codes. This paper will help to design universal XML browsers

ACCUMULATION AND TOXICITY of MIXTURE of LEAD AND ZINC IN CLAM RUDITAPES PHILIPPINARUM A.

研究了铅、锌对菲律宾蛤仔的急性毒性，以及铅、锌混合液在菲律宾蛤仔体内的积累和对过氧化氢酶酶活性的影响.结果表明：铅的毒性略大于锌的毒性（96HlC50值分别为14.28，16.40Mg/l）：铅、锌混合液对蛤仔体内的重金属吸收存在交互作用，高浓度的锌（6Mg/l）明显地抑制蛤仔对高浓度铅的吸收；铅和锌的浓度为（3Mg/l）时对蛤仔的重金属吸收均有促进作用；高浓度的铅和锌混合液对蛤仔过氧化酶活性亦有显著的交互作用.呈拮抗效应.Accumulation of mixture of lead and zinc and their toxic effect on the activities of Catalase (CAT)in clam following various exposure concentration were studied in laboratory.The 96h LC50 values of lead andzinc for clam were 14.28 mg/L.16.40mg/L, respectively.the toxicities of two kinds of metals were in sequence of Ph＞Zn.Lead and zinc bioaccumulated in the study showed that different organ has different capacities for accumulating metal.with high levels in the gill.followed by soft body.and comparative lesseramounts in the fluid of cavity.The ratio of accumulation of lead in gill and soft body was far larger than thatof zinc.The clam was strong accumulator of lead.while was weak accumulator of zinc.The L9 (34)factortests showed a significant interaction between Zn and Ph on the activities of CAT in clam.indicated the antagonistic effect of Ph and Zn of CAT activities

反复分批发酵法制备甘油过程中的影响因素

对游离耐高渗酵母细胞反复分批发酵生产甘油的影响因素进行研究，结果表明：反复分批发酵过程中所需的最优玉米浆浓度低于普通的分批发酵；较好的菌体反复利用时机确定为发酵液中残糖降至约1％，即细胞活性为60％时；在菌体的反复利用过程中残留在菌体中的代谢产物的累积会对甘油发酵产生的抑制作用，每次菌体利用之前用无菌水洗涤菌体一遍可消除抑制

反复分批发酵法制备甘油过程中的影响因素

对游离耐高渗酵母细胞反复分批发酵生产甘油的影响因素进行研究 ,结果表明 :反复分批发酵过程中所需的最优玉米浆浓度低于普通的分批发醇 ;较好的菌体反复利用时机确定为发酵液中残糖降至约 1% ,即细胞活性为 6 0 %时 ;在菌体的反复利用过程中残留在菌体中的代谢产物的累积会对甘油发酵产生抑制作用 ,每次菌体利用之前用无菌水洗涤菌体一遍可消除抑

反复分批发酵法制备甘油过程中的影响因素

对游离耐高渗酵母细胞反复分批发酵生产甘油的影响因素进行研究，结果表明：反复分批发酵过程中所需的最优玉米浆浓度低于普通的分批发酵；较好的菌体反复利用时机确定为发酵液中残糖降至约1％，即细胞活性为60％时；在菌体的反复利用过程中残留在菌体中的代谢产物的累积会对甘油发酵产生的抑制作用，每次菌体利用之前用无菌水洗涤菌体一遍可消除抑制