The Influence of Magnesium Concentration for the Survival Rate of Bufo melanostictus Tadpoles in the Acidity Water and Alkaline Water

[目的]研究野外黑眶蟾蜍(Bufo melanostictus)蝌蚪的生存状况。[方法]研究分别在碱性水(p H 7.5~8.0)以及酸性水(p H 5.5~6.0)2种模拟环境条件下,通过急性毒性试验方法以不同的浓度梯度Mg~(2+)对黑眶蟾蜍蝌蚪进行24和48 h的胁迫试验。[结果]在相同浓度梯度和相同观察时间下,黑眶蟾蜍蝌蚪在碱性水中的存活率低于酸性水;不同p H的水环境与Mg~(2+)的协同作用显著影响黑眶蟾蜍蝌蚪的48 h存活率(F7,32=3.373,P<0.05);黑眶蟾蜍蝌蚪在酸性水环境下适宜生存的Mg~(2+)浓度范围为1 418.41~1 590.54 mg/L,半致死浓度(LC_(50))为2 357.32 mg/L,安全浓度为521.51 mg/L;在碱性水环境下适宜生存的Mg~(2+)浓度范围为1 264.91~1 418.41 mg/L,LC_(50)为1 879.81 mg/L,安全浓度为533.99 mg/L。[结论]该研究可为今后黑眶蟾蜍的保护提供理论依据。[Objective]The aim was to study the survival status of Bufo melanostictus tadpoles in the field.[Method]The regular acute toxicity test was carried out that Bufo melanostictus tadpoles were breed in the alkaline water( p H 7.5-8.0) and acidity water( p H 5.5-6.0)with different Mg~(2+) concentration gradient for 24 and 48 h.[Result]The results showed that the survival rate of Bufo melanostictus tadpoles in the alkaline water was lower than the acidity water in the same condition.Synergistic effects of different p H and Mg~(2+) concentration had a significant effect on the survival rate of Bufo melanostictus tadpoles in the 48 h toxicity experiment( F7,32= 3.373,P < 0.05).In the acidity water environment,48 h toxicity experiment results showed that the optimal Mg~(2+) concentration was 1 418.41-1 590.54 mg / L,the median lethal concentration( LC_(50)) was 2 357.32 mg/L and the safe concentration was 521.51 mg/L.By contract,these three indexes in the alkaline water environment were 1 264.91-1 418.41,1 879.81 and 533.99 mg / L,respectively.[Conclusion] The study can provide theoretical basis for protection of Bufo melanostictus.国家自然科学基金项目(31271124);; 广东省自然科学基金项目(s2013010016062

经管类专业本科生会计类课程体系构想

由厦门大学会计发展研究中心提供的第四届会计与财务问题国际研讨会——会计教育改革与发展论文集中的第二部分：教学研究会计类课程是经营管理类本科生的重要专业基础课，对培养经管类人才的会计素质有着十分重要的战略意义，课程体系建设对保证课程质量十分重要。本文从经管类本科生的会计类课程需要出发，对会计类课程的内容进行了比较分析，找到了各门课程的交叉重合部分，在此基础上对经管类本科生的会计类课程体系建设提出了初步构想

Therapeutic effects comparison between platelet-rich plasma and sodium hyaluronate intra-articular injection in treatment of knee osteoarthritis

目的关节腔内注射自体富血小板血浆（PRP）与透明质酸钠（SH）,观察并比较其疗效。方法于2012年2月至12月,漳州市解放军第175医院骨科收治的81例Kellgren-Lawrence（KL）分级标准为Ⅱ、Ⅲ级膝关节骨关节炎的患者,年龄48～60岁,平均（52.6±3.6）岁;男性27例,女性53例;左膝38例,右膝43例。K-L分级标准,Ⅱ级47例,Ⅲ级34例,按就诊顺序序贯随机平行分为SH组（40例）和PRP组（41例）,分别采用SH和PRP关节腔内注射进行治疗。治疗前和治疗后3、6、9、12个月对患者进行随访并进行美国西部Ontario与Mc Master大学骨关节炎指数（WOMAC）评分评估,两组间各时间点WOMAC评分比较采用t检验,两组内各时间点WOMAC评分比较采用重复测量方差分析;分别于治疗前和治疗后12个月进行膝关节磁共振检查,评价关节腔内积液的吸收情况。结果治疗前及治疗后3个月PRP组WOMAC评分[（48.42±4.95）分,（25.38±5.12）分]和SH组[（47.17±5.12）分,（25.17±5.09）分]比较,t值分别为-0.625和0.413,差异无统计学意义（P均〉0.05）;治疗后6、9、12个月PRP组WOMAC评分[（21.44±4.73）分,（20.54±4.46）分,（16.53±7.35）分]和SH组[（25.87±5.16）分,（27.22±4.21）分,（29.63±8.21）分]比较,PRP组低于SH组,t值分别为-2.765、-3.447和-4.657,差异具有统计学意义（P均〈0.05）,治疗后12个月复查磁共振,可见PRP组的关节积液吸收例数高于SH组,差异有统计学意义（P〈0.05）。结论膝关节腔内注射自体PRP,临床效果优于透明质酸钠,中长期疗效好。Objective To compare the efficacy of platelet rich plasma （PRP） and sodium hyaluronate （SH） intra-articular injection in the patients with knee osteoarthritis of grade Ⅱand Ⅲ. Methods A total of 81 patients with knee osteoarthritis of grade Ⅱ and Ⅲ （ Kellgren-Lawrence standard） were randomly assigned to the PRP and SH group. All 81 patients were treated in the 175th hospital of PLA, Zhangzhon, with the average age of （52.6 ± 3.6） years. There were 27 males and 53 females. Forty cases in SH group and 41 cases in PRP group had got the complete follow-up. Both the two groups adopted 4 ml of 5% lidocaine for intra-articular injection. All the patients were prospectively evaluated before the treatment and at the 34 , 6th , 9th and 12th month after the treatment by the Western Ontario and McMaster Universities （WOMAC） osteoarthritis index. The swelling subsiding knee was observed. Between the two groups WOMAC scores were compared using t test at each time point, within the groups the scores were compared by repeated measures analysis of variance. The absorption of accumulated fluid in the joint cavity was observed and compared by MRI before and 12 months after the treatment. Results No serious adverse event was detected in the two groups. According to the follow-up evaluations, both the groups presented clinical improvements. The comparison between the two groups showed no statistically significant difference before therapy and three months after the treatment （ t =- 0. 625, 0. 413, P 〉 0. 05 ）. Six months, nine months and 12 months after the treatment, WOMAC score in the PRP group[ （21.44 ±4. 73）, （20. 54 ± 4. 46） ,（16. 53 ± 7. 35 ） pointsl were lower than the SH group[（25.87 ±5.16）, （27.22 ±4.21）, （29. 63 ± 8.21 ） points] ; the difference was statistically signifieant（ t = - 2. 765, - 3. 447, - 4. 657, P 〈 0. 05 ）. Conclusion PRP intra-artieular injection in the treatment of knee osteoarthritis of gradeⅡ and Ⅲ may be more suitable compared to南京军区医学科技创新重点课题项目（15ZD027

碳酸盐岩油藏缝洞单元离散数值模型及压力响应曲线特征

缝洞型碳酸盐岩油藏储集体类型多样,如何充分利用试井测试资料认识钻遇储层特征是后期挖潜增产的关键,并且常规解析试井模型中无法考虑井与缝洞的位置关系导致解释结果误差较大。为此,根据缝洞单元内缝洞与井的分布特征,分别建立了井钻遇和未钻遇缝洞2种离散数值试井模型,并进行有限元求解,分析了缝洞体大小以及缝洞体与井之间距离对井底压力响应曲线的影响。采用该方法对塔里木哈拉哈塘油田14口井测试资料进行试井分析,结果表明,受缝洞体的影响,井底压力响应曲线特征可分为：缝洞发育-未钻遇缝洞-井在缝洞附近、缝洞发育-钻遇缝洞、钻遇多个连通的缝洞、基质为主-缝洞不发育以及缝洞不发育-钻遇个别缝洞5种类型

The Intersexual Differences of Sexual Dimorphism,Feeding Habits and Locomotor Performance at Different Temperatures of Southern Grass Lizard(Takydromus sexlineatus) in Zhaoqing,China

通过测量头、尾、体和胃检,研究肇庆南草蜥TAkydrOMuS SEXlInEATuS个体发育过程中两性异形和食性差异。南草蜥成体个体两性大小无显著差异,雄性头部显著大于雌性,两性头部大小异形可能是受到性选择压力的结果。雌雄个体食物生态位重叠度较高,两性生态位差异微弱,食性差异与两性头部异形无明显相关性。通过设计13种不同的环境温度,研究体温变化对南草蜥两性个体运动能力表现的影响。结果显示环境温度通过影响体温而影响南草蜥的运动表现,两性个体疾跑速均具有在低体温范围内随体温升高而加快、在高体温范围内随体温升高而降低的模式。在多数测试温度下,雄体的疾跑速均略大于雌体,但两者平均值在统计上无显著差异。体温对南草蜥的最大持续运动距离和停顿次数的影响显著,两性个体的疾跑速和最大持续运动距离呈显著正相关。雄性和雌性分别在26℃和30℃具有最佳的运动表现。Southern grass lizard( Takydromus sexlineatus) was collected from Zhaoqing,Guandong province to determine the sexual dimorphism and dietary habits by measuring the lengths of head,tail and body,and examining the items in the stomach.The results showed that there was no significant difference in body size.However,male individuals had significantly larger head size than females,which might be due to the divergent sexual selection pressure.A relatively high food niche overlap was observed between the sexes.There was little relationship between food habits and head dimorphism.By designing 13 different ambient temperatures,we determined the relationship between body temperature and locomotor performance of the adults.Ambient temperature affected locomotor performance by influencing the body temperature.Sprint speed increased with increasing body temperature at lower body temperature,while decreasing with increasing body temperatures at higher body temperatures,which was similar between the sexes.However,males ran slightly faster than females in most cases,but intersexual difference of sprint speed was not statistically significant.Body temperature also influenced the maximal move distance and pause times.Sprint speed was positively correlated with the maximal move distance.The optimal temperatures of locomotor performance differed between the sexes: about 26℃ for males and 30 ℃ for females,respectively.国家自然科学基金资助项目(31271124); 广东省自然科学基金(06300177); 广东省高等教育教学改革项目(粤财教[2012]361号); 大学生创新性实验计划项目(DC201316)共同资

The Intersexual Differences of Sexual Dimorphism,Feeding Habits and Locomotor Performance at Different Temperatures of Southern Grass Lizard(Takydromus sexlineatus) in Zhaoqing,China

通过测量头、尾、体和胃检,研究肇庆南草蜥TAkydrOMuS SEXlInEATuS个体发育过程中两性异形和食性差异。南草蜥成体个体两性大小无显著差异,雄性头部显著大于雌性,两性头部大小异形可能是受到性选择压力的结果。雌雄个体食物生态位重叠度较高,两性生态位差异微弱,食性差异与两性头部异形无明显相关性。通过设计13种不同的环境温度,研究体温变化对南草蜥两性个体运动能力表现的影响。结果显示环境温度通过影响体温而影响南草蜥的运动表现,两性个体疾跑速均具有在低体温范围内随体温升高而加快、在高体温范围内随体温升高而降低的模式。在多数测试温度下,雄体的疾跑速均略大于雌体,但两者平均值在统计上无显著差异。体温对南草蜥的最大持续运动距离和停顿次数的影响显著,两性个体的疾跑速和最大持续运动距离呈显著正相关。雄性和雌性分别在26℃和30℃具有最佳的运动表现。Southern grass lizard( Takydromus sexlineatus) was collected from Zhaoqing,Guandong province to determine the sexual dimorphism and dietary habits by measuring the lengths of head,tail and body,and examining the items in the stomach.The results showed that there was no significant difference in body size.However,male individuals had significantly larger head size than females,which might be due to the divergent sexual selection pressure.A relatively high food niche overlap was observed between the sexes.There was little relationship between food habits and head dimorphism.By designing 13 different ambient temperatures,we determined the relationship between body temperature and locomotor performance of the adults.Ambient temperature affected locomotor performance by influencing the body temperature.Sprint speed increased with increasing body temperature at lower body temperature,while decreasing with increasing body temperatures at higher body temperatures,which was similar between the sexes.However,males ran slightly faster than females in most cases,but intersexual difference of sprint speed was not statistically significant.Body temperature also influenced the maximal move distance and pause times.Sprint speed was positively correlated with the maximal move distance.The optimal temperatures of locomotor performance differed between the sexes: about 26℃ for males and 30 ℃ for females,respectively.国家自然科学基金资助项目(31271124); 广东省自然科学基金(06300177); 广东省高等教育教学改革项目(粤财教[2012]361号); 大学生创新性实验计划项目(DC201316)共同资

生物质与供氢甲醇共催化热解耦合集成优化制备芳烃的研究

为了提高目标产物的产率以及选择性,以HZSM-5介孔分子筛为催化剂,采用高有效氢碳比化合物——甲醇与生物质进行催化共热解,探讨热解温度、催化温度、有效氢碳比以及醇的种类对芳烃的产率、选择性以及催化剂的抗积碳性能的影响。结果表明,芳烃的产率以及选择性随着生物质与供氢试剂共催化热解时有效氢碳比的增加而显著增加,尤其是二甲苯的选择性,二者之间存在协同效应,当热解温度为400℃,催化温度550℃,甲醇的进样量为2 mL/min,氮气流速为200 mL/min时,其苯及其同系物等芳烃含量达到81.34%,单环芳烃(SBTXE)含量达到71.75%,而二甲苯的选择性达到40.81%,同时,供氢甲醇的添加提高了催化剂的抗结焦能力,使其石墨化焦炭含量增加。国家自然科学基金(31670599)云南省科技厅项目(2018FB071,2018FG001-054)云南省教育厅教师类项目(2018JS325)云南省大学生创新创业项目(S201710677016

应用ELISA 定量检测转基因玉米中

[中文文摘]应用纯化的Bt1杀虫晶体蛋白作为标准蛋白和免疫抗原,通过抗体-抗原-酶标抗体反应,建立了酶联免疫吸附测定法(ELISA),以定量检测转基因玉米中的Bt1表达蛋白。用建立的ELISA法对4种进口玉米产品进行了测定,实验结果得到了免疫印迹分析的验证,并与进口试剂盒方法的定量分析结果相一致,因而建立的ELISA法具有操作简便、快速特异、定量准确、经济实惠的优点,特别适合于大批量检测,有着良好的应用前景。[英文文摘]Basedontheantibody-antigen-enzymeantibodyreaction andbyusingpuriftiedBt1insecticidalcrystalproteinas both standard protein and immunity antigen, a quantitative enzyme-linked immunosorbent assay (ELISA) method was established to detect express Bt1 protein in genetically modified maize.4 imported maize samples were determined by ELISA, In comparison with the western-blotting & KIT assay method, the results were almost identical. It concluded that the ELISA methodmightbeusefulforeasy,rapid,reliableandeffectiveassayinlargesamples.厦门科技计划资助项目(350222001109

Current Progress and Development Trend of Woody Oil-plant Derived Biodiesel Production in China

作者简介: 杨文博(1985-) , 女, 河南安阳人, 硕士, 主要从事植物分子生物学科研工作。 通讯作者: 郑文明( 1964-) , 男, 河南永城人, 博士, 教授, 主要研究方向: 植物分子营养和基因工程。Email: zhengwenming@ yahoo.cn[中文文摘]木本油料植物生产生物柴油是适合中国国情的生物柴油发展策略之一。我国虽有丰富的木本油料植物资源,但是实施其开发和产业化都面临许多问题。分析了我国此项产业的现状,阐述了在资源调查、产学研结合、企业管理和政策法规等方面的问题和对策,总结了当前全球注重发展‘环保能源’的大背景下我国利用木本油料植物生产生物柴油的发展趋势。[英文文摘]The woody oil - plants derived biodiesel production is suitable for China"s biodiesel develop ment strategy Although China is rich in woody oil-plant resources, the implementation of its develop ment and industrialization is facing many problems.This paper systematically analyzed current status of the industry, described the issues and countermeasures in resource survey, production management, policies and gulations, summarized the trend of biodiesel production in China by using woody oil plants under the current background of global focus on the development of 'green energy '.河南省杰出青年基金项目(084100410025);河南省农业科技攻关项目(112102110112