An Hourglass-Type Spinal Schwannoma Spreading to the Chest Treated with One-Stage Total Removal through Posterior Paravertebral Approach without Opening the Pleural Cavity (Clinical Observation)

The article describes the treatment of a 20-year-old patient with a spinal hourglass schwannoma (neurinoma) in the thoracic spine, growing from the right Th3 spinal root, spreading into the thoracic cavity and compressing the apex of the right lung. The introduction presents the frequency of occurrence of such neoplasms among all spinal cord tumors. Complaints, neurological symptoms, neoplasm diagnosis methods and its clinical and radiological manifestations are presented. The article describes stages and techniques of total surgical removal of the spinal hourglass schwannoma through the posterior paravertebral minimally invasive approach without opening the pleural cavity and with using neurophysiological monitoring of spinal cord function performed at the Neurosurgical Clinic of the Novosibirsk Research Institute of Traumatology and Orthopaedics named after Ya.L. Tsivyan. The data  of histological examination and MRI control in the early postoperative period are presented. The follow-up was carried out at 6 and 12 months after surgical treatment. The publications describing the surgical treatment of paravertebral tumors through different approaches, the technique of their removal, possible complications and methods of their prevention are analyzed, and the classification according to Eden (1941), which characterizes the type of tumor growth, is presented

Iмплементація угоди про асоціацію Україна-ЭС: угода АСАА (The implementation of the Ukraine–European union association agreement: the agreement ACAA)

У статті проаналізовано Угоду про асоціацію між Україною та ЄС. Розглянуто зроблені кроки з боку України в імплементації Угоди. Проведено ретельний аналіз перспектив підписання Угоди АСАА у рамках імплементації Угоди про асоціацію. Окреслено необхідні заходи для досягнення нової угоди в рамках Угоди про асоціацію. (This article is an attempt to analyze the Ukraine-European Union Association Agreement. The steps of the Ukrainian side of the implementation of the Agreement are considered. There is made an attempt to conduct a thorough analysis of the possibility to sign the ACAA Agreement through the implementation of the Association Agreement. There are briefly outlined the necessary actions for getting a new agreement through the Association Agreement.

Morphological peculiarites and functional activity of adipose-derived mesenchimal stem cells during in vitro cultivation conditions

The studies were conducted on 2-3-months-old males of C57BL/6 mice weighing 20–24 g. Obtaining and cultivating of adipose-derived mesenchimal stem cells (AD MSCs) were carried out in a sterile laminar box with compliance of conditions of asepsis and antiseptics. AD MSCs of the 2, 4, 7 and 12 passages were analyzed. Morphometric analysis was performed using a light microscopy. Morphometric parameters such as cell and nucleus area or nuclear-cytoplasmic ratio (NCR) were calculated using the Axiovision light microscope (Carl Zeiss, Germany) and ImageJ 1.45 software. Trypan blue dye used for investigation of the viability of MSC. The morphological characteristics of mesenchymal stem cells from adipose tissue during the process of cultivation changes: at the first passages of cultivation, the cells are spindle-shaped with two, at least three, long long cytoplasmic processes, located bipolar. Near the nucleus the Golgi complex is clearly visible – a sign of active cells. At later passages cells have a small cytoplasmic processes and the bipolar arrangement of processes changes by stellar arrangement. Golgi complex is also clearly visualized. The indicator of the nuclear-cytoplasmic ratio in MSC from adipose tissue is significantly reduced at 7 passage to 0.2189 ± 0.0122 (P < 0.01), and at 12 passage to 0.1111 ± 0.0086 (P < 0.001) compared to the 2 passage. The coefficient of proliferation of MSC from adipose tissue is significantly reduced at 12th passage. The viability of mesenchymal stem cells from adipose tissue with an increasing of a number of passages significantly reduces and at the 12th passage of cultivation reaches 84,67 ± 1,36* (P < 0.05). The content of apoptotic cells that exhibited sensitivity to serum-free significantly increased at 7 and 12 passages and was respectively 21.33 ± 1.36 (P < 0.05) and 23.67 ± 0.97% (P < 0.05)

MENINGIOMAS OF THE CEREBELLAR TENTORIUM: THE RESULTS OF SURGICAL TREATMENT

Aim of the study was to evaluate the surgeries of tentorial meningiomas in Neurosurgical Department No.1 of the Novosibirsk Institute of Traumatology and Orthopedics. Material and methods. Study includes 64 patients with tentorial meningiomas of different location, treated surgically from 2008 to 2017. Among them 55 (85.9 %) were female patients and 9 were male (14.1 %) with average age of 62.9 years in females and 56.7 years in males. For diagnostic purposes, the patients were performed brain magnetic resonance imaging and/or computed tomography with or without contrast agents. In this series, 83 % of the tumors were of large or giant (with diameter of more than 4 cm). In 63 cases the tumors were typical meningiomas and only 1 case was a malignant anaplastic meningioma. Results. All patients were treated surgically in one or two steps. Overall, there were 70 standard microsurgical resections. Electromyography with Neuropack-2 («NIHON KOHDEN Corp.», Japan) was performed to control brain stem function during the surgery, with short latency auditory evoked potentials (SLAEPs) received. When sinus walls were affected, the tumor matrix was processed with 20W neodymium laser in coherent mode and ablation. In 63 cases, gross-total resection was achieved: Simpson I (76.6 %, n = 49) and Simpson II (21.8 %, n = 14). One patient (1.6 %) was treated with subtotal resection because of complicated surgical approach and massive transverse sinus and sinus confluence bleeding. Post-operative mortality was 3.1 % (2 cases). SLAEP data did not demonstrate any significant irritation or damage to the brain stem with performed surgical approaches. There were no infections of the surgical wound, meninges, brain or CSF leaks after the surgery. Discussion. Tentorial meningiomas are rare brain tumors treated with gross-total tumor resection when possible. Performed surgeries resulted in mild functional decrease in quality of life, but Karnofsky scale for each patient was returning to its original state in 2 weeks. Complications, including neurological disability, presented in 19 % (n = 12) of the patients, and post-operative mortality did not exceed the common literature data. Most of the focal neurological disabilities have disappeared in 2 weeks after the surgery

The influence of different pathogens on the lysozyme activity into tissues of rat oral cavity

Aim: To determine action of the different pathogens on the lysozyme activity into tissues of oral cavity and serum.Methods: The lysozyme activities was determined into oral mucosa cheek, tongue gum and serum of 158 white rats (11 series experiments). The pathogens were used: atropine, protamine sulfat, indometacyn, bee poison, hydrasine sulfat, cytostatic cyclofosfan, lincomycin, lipopolysaccharide, composition of antibiotic and omeprasol for ACBTResults: The  whole of pathogens decreased lysozyme activity (mean in 1,6-2,5 times) into oral tissues and on 16 % into serum. The specific lowering of lysozyme activities (Δ%/mg pathogen) was low most for lipopolysaccharide, especially after oral application usage (exceeding was in tens times).Conclusion: The lysozyme activity lowering may play significant role in pathogenesis of stomatologic diseases/ Lipopolysaccharide (LPS) send lysozyme activity lowering most especially after oral application. Probably, the antilysozyme action of pathogens realize by LPS. The stomatogenic factor in pathogenesis and profilactic of noninfection diseases is important

The experimental prophylaxis of the peroxide periodontitis by antidysbiotic means

Aim: To determine the periodontoprotective properties of antidysbiotic means (ADM) at the peroxide periodontitis.Methods: ADM (quertulyn, lequin, lecasil and lysozyme-forte) were used. The peroxide periodontitis was reproduced by peroxide sunflower oil introduction in during 2,5 month. The levels of MDA, elastase, urease, lysozyme, catalase were determined into gum. The levels of alkaline and acid phosphatase, calcium and protein were determined into periodontal bone.Results: The introduction of ADM the reduced the levels of inflammation markers (MDA and elastase) and to the degree of  dysbiosis but raised the levels of lysozyme and catalase into gum. ADM reduced the acid phosphatase activity but raised the alkaline phosphatase into bone.Conclusion: Lequin was more effective means as antiinflammation, quertulyn was more effective means as antidysbiotic action, lysozyme-forte was more effective means mineralization action. The lecitine contents means lequin and lecasil possessed more effective action on the degree mineralization of periodontal bone

IMMUNOMODULATORY EFFECT OF CIRCULATING BONE MARROW PROGENITORS AS A POSSIBLE MECHANISM OF NEUROPROTECTION IN TRAUMATIC BRAIN INJURY

We have previously shown that acute traumatic brain injury (TBI) is accompanied by increased level of circulating bone marrow progenitors, and favorable outcome is associated with early mobilization of CD34+CD45+ hematopoietic progenitor cells (HP). The present study was aimed at investigating whether patients with early HP mobilization differed from those with mobilization failure by systemic inflammatory reaction and immune parameters. The TBI patients were characterized by increased levels of serum C-reactive protein (CRP), IL-1в, IL-6, IL-8, MCP-1, G-CSF and IL-1ra indicative for presence of systemic inflammatory response. Importantly, patients with lacking mobilization of early HPs were shown to have significantly higher serum levels of CRP, MCP-1, MIP-1в, and G-CSF and a lower level of VEGF. In addition, patients with lack of early HP mobilization differed by significantly lower absolute number of lymphocytes, CD3+ T cells, CD4+ T cells, CD16+ NK cells and proliferative response of mononuclear cells to stimulation with ConA as well as by 4-fold higher rate of infectious complications compared with the opposite group. These data suggest that correlation of early mobilization of CD34+CD45+ cells with a favorable outcome in TBI patients may be partially mediated by anti-inflammatory and immunomodulatory effects of circulating bone marrow progenitors