Expansion of different subpopulations of CD26−/low T cells in allergic and non-allergic asthmatics

CD26 displays variable levels between effector (TH17 ≫ TH1 > TH2 > Treg) and naïve/memory (memory > naïve) CD4+ T lymphocytes. Besides, IL-6/IL−6R is associated with TH17-differentiation and asthma severity. Allergic/atopic asthma (AA) is dominated by TH2 responses, while TH17 immunity might either modulate the TH2-dependent inflammation in AA or be an important mechanism boosting non-allergic asthma (NAA). Therefore, in this work we have compared the expression of CD26 and CD126 (IL-6Rα) in lymphocytes from different groups of donors: allergic (AA) and non-allergic (NAA) asthma, rhinitis, and healthy subjects. For this purpose, flow cytometry, haematological/biochemical, and in vitro proliferation assays were performed. Our results show a strong CD26-CD126 correlation and an over-representation of CD26− subsets with a highly-differentiated effector phenotype in AA (CD4+CD26−/low T cells) and NAA (CD4−CD26− γδ-T cells). In addition, we found that circulating levels of CD26 (sCD26) were reduced in both AA and NAA, while loss of CD126 expression on different leukocytes correlated with higher disease severity. Finally, selective inhibition of CD26-mRNA translation led to enhanced T cell proliferation in vitro. These findings support that CD26 down-modulation could play a role in facilitating the expansion of highly-differentiated effector T cell subsets in asthma.This work was supported by grants from Sociedad Española de Neumología y Cirugía Torácica, (SEPAR) (121/2012) and Instituto de Salud Carlos III, Ministerio de Economía y Competitividad (Fondo de Investigación Sanitaria, FIS; co-financed by European Union ERDF funds) (PI13/02046). JJN-F is a recipient of a Xunta de Galicia Fellowship (co-financed by European Social Fund (ESF))S

Investigación de fenómenos psicosociales

The book “Investigación de fenómenos psicosociales”. An approach for the encounter with the other is an initiative of some researchers of the Psychology program of the Manizales Regional Center of the Catholic University Luis Amigó, due to the exponential growth of research in phenomena that shape the social reality of our context and the interest of problematizing research as an option of encounter with people and communities, rethinking traditional and extractivist notions in the construction of knowledge. With this initiative, it is evident that the research processes carried out throughout the years of existence of the Psychology program, have been interlocated, accompanied and fed back by academic agents from other institutions, which has significantly nurtured the discussions. In addition, the production presents a common denominator, and that is qualitative research, as a recurrent design in the different studies, becoming a device for the problematization of the text.illustratorEl libro Investigación de fenómenos psicosociales. Una aproximación para el encuentro con el otro es una iniciativa de algunos investigadores del programa de Psicología del Centro Regional Manizales de la Universidad Católica Luis Amigó, en virtud del crecimiento exponencial de la investigación en fenómenos que configuran la realidad social de nuestro contexto y el interés de problematizar la investigación como una opción de encuentro con personas y comunidades, replanteando nociones tradicionales y extractivistas en la construcción de conocimiento. Con esta iniciativa se evidencia que los procesos de investigación realizados a través de los años de existencia del programa de Psicología, se han visto interlocutados, acompañados y retroalimentados por agentes académicos de otras instituciones, lo que ha nutrido significativamente las discusiones. Además, la producción presenta un común denominador, y es la investigación cualitativa, como diseño recurrente en los diferentes estudios, convirtiéndose en un dispositivo para la problematización del texto

Higher COVID-19 pneumonia risk associated with anti-IFN-α than with anti-IFN-ω auto-Abs in children

We found that 19 (10.4%) of 183 unvaccinated children hospitalized for COVID-19 pneumonia had autoantibodies (auto-Abs) neutralizing type I IFNs (IFN-alpha 2 in 10 patients: IFN-alpha 2 only in three, IFN-alpha 2 plus IFN-omega in five, and IFN-alpha 2, IFN-omega plus IFN-beta in two; IFN-omega only in nine patients). Seven children (3.8%) had Abs neutralizing at least 10 ng/ml of one IFN, whereas the other 12 (6.6%) had Abs neutralizing only 100 pg/ml. The auto-Abs neutralized both unglycosylated and glycosylated IFNs. We also detected auto-Abs neutralizing 100 pg/ml IFN-alpha 2 in 4 of 2,267 uninfected children (0.2%) and auto-Abs neutralizing IFN-omega in 45 children (2%). The odds ratios (ORs) for life-threatening COVID-19 pneumonia were, therefore, higher for auto-Abs neutralizing IFN-alpha 2 only (OR [95% CI] = 67.6 [5.7-9,196.6]) than for auto-Abs neutralizing IFN-. only (OR [95% CI] = 2.6 [1.2-5.3]). ORs were also higher for auto-Abs neutralizing high concentrations (OR [95% CI] = 12.9 [4.6-35.9]) than for those neutralizing low concentrations (OR [95% CI] = 5.5 [3.1-9.6]) of IFN-omega and/or IFN-alpha 2

New Findings in the Signaling Pathways of cis and trans Platinum Iodido Complexes' Interaction with DNA of Cancer Cells

We have selected a series of aliphatic amine platinum compounds bearing chloride and/or iodide as the leaving groups. The complexes’ cytotoxicity and interaction with DNA indicated differences in the reactivity. Now, we are reporting on the analysis of their molecular mechanism of action on gastric cancer cells. Our data reveals differences between them. Chlorido drugs showed similar behavior to cisplatin; they both required p53 to induce apoptosis but only cis-ipa showed DNA damage requirement for apoptosis induction. On the contrary, cis and trans iodido induced cell death independent of p53 activity, and they induced cell death through Bid activation, so their toxicity could be enhanced in a combined treatment with novel Bcl-2 protein family inhibitors. We also report the structural features of the DNA adduct for one of the complexes by X-ray diffraction. These findings represent a step forward in the search for new platinum-derived agents more specific and effective in the treatment of cancer.Authors A.G.Q., M.C., and A.A.-V. received funding from MINECO CTQ2015-68779-R. I.S.P. received funding from P17-01401 (supported by FEDER funds) from Fondo de Investigaciones Sanitarias, Instituto de Salud Carlos III, SpainNPL is a fellow of the Programa de Doctorado Biociencias Moleculares UAM, Madrid, Spain. NPL was supported by a fellowship Programa de Formación de Profesorado Universitario REF: FPU15/04669

Reactions of palladium (II) chloride with 1,4 - diphenyl - 2,3 - dimethyl - 1,4 - diazabutadiene and 1,4-DI(p-methoxyphenyl)-2,3-dimethyl- 1,4 - diazabutadiene

The reactions of palladium(II) chloride with 1,4 - diphenyl - 2,3 - dimethyl - 1,4 - diazabutadiene and 1,4 - di(p - methoxyphenyl) - 2,3 - dimethyl - 1,4 - diazabutadiene are described. With 1,4 - diphenyl - 2,3 - dimethyl - 1,4 - diazabutadiene diimine fission is produced, giving rise to a product identified by elemental analysis, IR and Raman spectra, and X-ray diffraction, as trans-dichlorobis(aniline) palladium(II). The complex is soluble in dimethylformamide and crystallizes with two molecules of solvent. The substance crystallizes in the monoclinic space group P21/n. The X-ray data were refined to R = 0.047 and Rw = 0.046. Final distances are PdN = 2.060(5) Å and PdC1 = 2.299(2) Å. There are two bifurcated intermolecular NH ... C1 and CH... C1 hydrogen bonds which, together with one more intermolecular hydrogen bond NH... O, are responsible for the packing of the molecules. However, when 1,4 - di(p - methoxyphenyl) - 2,3 - dimethyl - 1,4 - diazabutadiene was treated with palladium chloride under the same conditions cis - dichloro - 1,4 - di(p - methoxyphenyl) - 2,3 - dimethyl - 1,4 - diazabutadiene was formed, as deduced from elemental analysis, and IR and Raman spectra. © 1987

Oxidation of anticancer Pt(ii) complexes with monodentate phosphane ligands: Towards stable but active Pt(iv) prodrugs

The synthesis, characterization and cytotoxicity studies of two novel platinum(iv) complexes, trans-PtCl4(dma)(PPh3), 1, and trans-PtCl4(ipa)(PPh3), 2, where dma is dimethylamine and ipa is isopropylamine, have been carried out. Both complexes contain aliphatic amines trans to phosphane ligands as a good alternative to take advantage of the phosphane group lipophilicity and the stability of platinum(iv) to obtain more effective drugs. Moreover, the complexes are stable in solution and such stability allowed their antitumoral action and DNA interaction to be checked and proved. © 2013 The Royal Society of Chemistry.Peer Reviewe

Estudio interdisciplinar en torno a una fuente literaria (Pío Baroja: Noches del Buen Retiro) : El Madrid de Pío Baroja

Proyecto que pretende que el alumno investigue y llegue al conocimiento del Madrid de Pío Baroja, tomando como base su obra 'Las Noches del Buen Retiro'. Se estudiará de forma interrelacionada el medio físico (Madrid y el Retiro), institucional (el sistema de la Restauración), económico (la Revolución industrial), social (la sociedad madrileña y la mujer) y cultural (corrientes filosóficas y artísticas). Las principales actividades son: visitas extraescolares, mesas redondas, audiciones de ópera y zarzuela, dramatización de una obra teatral, concurso-exposición de pintura y fotografía, artículos periodísticos y proyección de vídeos sobre el Madrid de Baroja. Se concluye con los recursos utilizados y los criterios de evaluación..Madrid (Comunidad Autónoma). Consejería de Educación y CulturaMadridMadrid (Comunidad Autónoma). Subdirección General de Formación del Profesorado. CRIF Las Acacias; General Ricardos 179 - 28025 Madrid; Tel. + 34915250893ES