Bortezomib-containing multimodality treatment for antibody-mediated rejection with anti-HLA and anti-AT1R antibodies after kidney transplantation

For decades, the human leukocyte antigen (HLA) complex has been considered the primary target of antibody-mediated rejection (AMR), and treatment strategies have mainly focused on anti-HLA antibodies. Recently, other antibodies potentially causing organ damage and loss have been discovered. Conclusive evidence on treatment options for these subtypes of AMR is still lacking. After an experience previously reported in this journal,1 we describe a case of late-onset AMR, with mixed anti-HLA and anti-angiotensin II type 1 receptor (AT1R) antibodies, that was successfully treated with a multimodal approach, including the use of the proteasome inhibitor bortezomib

Role of perfusion machines in the setting of clinical liver transplantation. A qualitative systematic review

Growing enthusiasm around machine perfusion (MP) in clinical liver transplantation (LT) may be the preamble for standardized practice to expand the donors' pool. The present systematic review investigated all the liver transplantations performed using grafts treated with MP. A systematic review of 309 papers was performed. Eventually, 27 articles were enrolled for the study. A total number of 173 cases was reported. Only 12 cohort studies were identified: the remaining ones were case reports or case series. Hypothermic machine perfusion was performed in 102 (59.0%), normothermic machine perfusion in 65 (37.6%), and controlled oxygenated rewarming in the remaining 6 (3.4%) cases. Donor characteristics, evaluation of graft quality and end-points were not homogeneous among the studies. Overall, post-LT results were excellent, with 1.2 and 4.0% of patients experienced primary non-function and ischemic-type biliary lesions, respectively

Emotional management and biological markers of dietetic regimen in chronic kidney disease patients

The aim of the study was to investigate the association between psychological characteristics and biological markers of adherence in chronic kidney disease patients receiving conservative therapy, hemodialysis, peritoneal dialysis (PD), or kidney transplantation. Seventy-nine adult patients were asked to complete the following questionnaires: Toronto Alexithymia scale, Snaith–Hamilton Pleasure Scale, and Short Form Health Survey. Biological markers of adherence to treatment were measured. Peritoneal dialysis patients showed a lower capacity to feel pleasure from sensorial experience (p = .011) and a higher values of phosphorus compared to the other patients’ groups (p = .0001). The inability to communicate emotions was negatively correlated with hemoglobin levels (r = −(0).69; p = .001) and positively correlated with phosphorus values in the PD patients (r = .45; p = .050). Findings showed higher psychological impairments and a lower adherence to the treatment in PD patients and suggest the implication of emotional competence in adherence to treatment.The aim of the study was to investigate the association between psychological characteristics and biological markers of adherence in chronic kidney disease patients receiving conservative therapy, hemodialysis, peritoneal dialysis (PD), or kidney transplantation. Seventy-nine adult patients were asked to complete the following questionnaires: Toronto Alexithymia scale, Snaith-Hamilton Pleasure Scale, and Short Form Health Survey. Biological markers of adherence to treatment were measured. Peritoneal dialysis patients showed a lower capacity to feel pleasure from sensorial experience (p = .011) and a higher values of phosphorus compared to the other patients' groups (p = .0001). The inability to communicate emotions was negatively correlated with hemoglobin levels (r = -(0).69; p = .001) and positively correlated with phosphorus values in the PD patients (r = .45; p = .050). Findings showed higher psychological impairments and a lower adherence to the treatment in PD patients and suggest the implication of emotional competence in adherence to treatment

Novel non-aqueous amine solvents for reversible CO2 capture

Abstract This study describes the chemical capture of CO2 by AMP and some AMP–alkanolamine blends, namely IPMEA, TBMEA and MDEA, in non-aqueous solvents. The solvents used are a mixture of ethylene glycol and 1-propanol or single diethylene glycol monomethyl ether. A gas mixture containing 15% of CO2 in air was used for the experiments. We designed two different experimental procedures: 1) batch experiments of CO2 absorption and desorption aimed at investigating the absorption and desorption properties of the different absorbents; 2) continuous cycles of CO2 absorption (20 °C) and desorption (90-95 °C) in packed columns in order to investigate the CO2 absorption efficiency. The CO2 removal efficiency at equilibrium was in the range 87-95% depending on the operational conditions. The identification and the quantification of the species in solution were obtained from 13C NMR spectroscopic analysis

Linear, redox modified DNA probes as electrochemical DNA sensors

We show here that hybridization-linked changes in the dynamics of a redox-modified, electrode-bound linear (as opposed to stem-loop) probe DNA produce large changes in Faradaic current, allowing for the ready detection of target oligonucleotides

Donor-to-recipient gender match in liver transplantation. A systematic review and meta-analysis

AIM To perform a systematic review and meta-analysis on donor-to-recipient gender mismatch as a risk factor for post-transplant graft loss. METHODS A systematic literature search was performed using PubMed, Cochrane Library database and EMBASE. The primary outcome was graft loss after liver transplantation. Odds ratios and 95% confidence intervals were calculated to compare the pooled data between groups with different donor-to-recipient gender matches. Three analyses were done considering (1) gender mismatches (F-M and M-F) vs matches (M-M and F-F); (2) Female-to-Male mismatch vs other matches; and (3) Male-to-Female mismatch vs other matches. RESULTS A total of 7 articles were analysed. Gender mismatch (M-F and F-M) was associated with a significant increase of graft loss respect to match (M-M and F-F) (OR: 1.30; 95%CI: 1.13-1.50; P < 0.001). When F-M mismatch was specifically investigated, it confirmed its detrimental role in terms of graft survival (OR: 1.83; 95%CI: 1.20-2.80; P = 0.005). M-F mismatch failed to present a significant role (OR: 1.09; 95%CI: 0.73-1.62; P = 0.68). CONCLUSION Gender mismatch is a risk factor for poor graft survival after liver transplantation. Female-to-male mismatch represents the worst combination. More studies are needed with the intent to better clarify the reasons for these results

Reducing error and measurement time in impedance spectroscopy using model based optimal experimental design

In this work we introduce several novel tools for the reduction of errors in parameters estimated with electrochemical impedance spectroscopy experiments. An optimization strategy is developed that minimizes an estimate of the errors on the parameters while bounding the experimental time. The approach is also used to reduce experimental time while keeping a bound on the parameter errors. This feature is particularly critical in systems changing significantly within the experimental time. The paper uses a fuel cell electrode model to test this methodology and presents a real time algorithm for coupling experiment with the parameter estimation and experimental optimization

Platelet-to-lymphocyte ratio in the setting of liver transplantation for hepatocellular cancer. A systematic review and meta-analysis

AIM: To perform a systematic review and meta-analysis on platelet-to-lymphocyte ratio (PLR) as a risk factor for post-transplant hepatocellular cancer (HCC) recurrence. METHODS: A systematic literature search was performed using PubMed. Participants of any age and sex, who underwent liver transplantation for HCC were considered following these criteria: (1) studies comparing pre-transplant low vs high PLR values; (2) studies reporting post-transplant recurrence rates; and (3) if more than one study was reported by the same institute, only the most recent was included. The primary outcome measure was set for HCC recurrence after transplantation. RESULTS: A total of 5 articles, published between 2014 and 2017, fulfilled the selection criteria. As for the quality of the reported studies, all the investigated articles presented an overall high quality. A total of 899 cases were investigated: 718 cases (80.0%) were males. Three studies coming from European countries and one from Japan presented HCV as the main cause of cirrhosis. On the opposite, one Chinese study presented a greater incidence of HBV-related cirrhotic cases. In all the studies apart one, the PLR cut-off value of 150 was reported. At meta-analysis, high PLR value was associated with a significant increase in recurrence after transplantation (OR = 3.33; 95%CI: 1.78-6.25; p &lt; 0.001). A moderate heterogeneity was observed among the identified studies according to the Higgins I 2 statistic value. CONCLUSION: Pre-transplant high PLR values are connected with an increased risk of post-operative recurrence of hepatocellular cancer. More studies are needed for better clarify the biological mechanisms of this results

The Initial Conditions of Clustered Star Formation. II. N2H+ Observations of the Ophiuchus B Core

We present a Nobeyama 45 m Radio Telescope map and Australia Telescope Compact Array pointed observations of N2H+ 1-0 emission towards the clustered, low mass star forming Oph B Core within the Ophiuchus molecular cloud. We compare these data with previously published results of high resolution NH3 (1,1) and (2,2) observations in Oph B. We use 3D Clumpfind to identify emission features in the single-dish N2H+ map, and find that the N2H+ `clumps' match well similar features previously identified in NH3 (1,1) emission, but are frequently offset to clumps identified at similar resolution in 850 micron continuum emission. Wide line widths in the Oph B2 sub-Core indicate non-thermal motions dominate the Core kinematics, and remain transonic at densities n ~ 3 x 10^5 cm^-3 with large scatter and no trend with N(H2). Non-thermal motions in Oph B1 and B3 are subsonic with little variation, but also show no trend with H2 column density. Over all Oph B, non-thermal N2H+ line widths are substantially narrower than those traced by NH3, making it unlikely NH3 and N2H+ trace the same material, but the v_LSR of both species agree well. We find evidence for accretion in Oph B1 from the surrounding ambient gas. The NH3/N2H+ abundance ratio is larger towards starless Oph B1 than towards protostellar Oph B2, similar to recent observational results in other star-forming regions. Small-scale structure is found in the ATCA N2H+ 1-0 emission, where emission peaks are again offset from continuum emission. In particular, the ~1 M_Sun B2-MM8 clump is associated with a N2H+ emission minimum and surrounded by a broken ring-like N2H+ emission structure, suggestive of N2H+ depletion. We find a strong general trend of decreasing N2H+ abundance with increasing N(H2) in Oph B which matches that found for NH3.Comment: 55 pages (manuscript), 15 figures, ApJ accepte

United-and-Close: An interactive visual platform for assessing urban segregation within the 15-minutes paradigm

The ‘15-minute city’ paradigm is an urban model based on the concept of ‘hyper-proximity’: citizens should be able to access fundamental services and facilities (such as schools, shops, parks, doctors, and markets) within 15-20 minutes on foot, by bicycle or by public transport. Compliance with the ‘15-minute city’ paradigm is supposed to reduce pollution and social inequalities. It is supposed to bring the psychological fragility of the citizen back to the center of the urban redevelopment debate. Although the concept has gained great attention and interest from policymakers and urban designers, we still lack tools that can help to validate, on a data-driven basis, the assumption that hyper-proximity is eventually correlated with lower urban segregation, which is one of the driving forces that lead to social inequalities. We aim to define a data-driven methodology to analyze the urban areas where services should be accessible within 15 minutes; network analysis is exploited to estimate services proximity as well as the connectivity of different urban areas with each other, in order to gather signals of the general resilience or exposure to urban segregation. We also aim to compute a set of city-agnostic metrics that will include user-specified parameters and personalized weights for each Point of Interest’s category. United-and-Close is the resulting Web platform designed to be accessible to citizens, policy and decision-makers, and investors, but also for researchers involved in disciplines such as urban informatics that need support to better assess the 15-minute paradigm and its actual impact on our cities