Microbial quality and yeast population dynamics in cracked green table olives' fermentations

Cracked green table olives, from the Manzanilla variety, are a fermented food produced and consumed in Portugal. The objective of the present work was to study the microbiological characteristics and yeast population evolution during the fermentation of cracked green olives. The predominant microorganisms were yeasts while lactic acid bacteria were not detected and a clear decrease of the mould population was observed. At the end of the fermentations, no viable counts of Enterobacteriaceae were found. Yeast isolates were identified by the 5.8S rRNA-ITS region restriction analysis and by sequencing the D1/D2 region of the 26S rRNA gene. During the initial phases of the fermentations a great diversity of yeasts was observed. However, as the processes evolved the biodiversity decreased with the fermentative yeasts Citeromyces matritensis, Zygotorulaspora mrakii and Saccharomyces cerevisiae becoming the dominant species. The presence of these fermentative yeasts at the end of the production process is associated to a risk of spoilage. The results obtained represent a first attempt towards the comprehension of the microbiota of this type of “Natural olives” that constitute an important component of the Mediterranean diet

Modelling the exposure to Cronobacter sakazakii by consumption of a cocoa-milk-based beverage processed by pulsed electric fields

peer-reviewedM.C. Pina-Pérez is grateful to CSIC for providing a DOCTOR contract linked to the INNPACTO project IPT-2011-1724-060000. This study was carried out with funds from BISOSTAD project PSE-060000-2009-003, Generalitat Valenciana I+D+I emergent research groups GV/2010/064 and CYCIT project AGL2010-22206-C02-01.Infants’ exposure (Nf ) to Cronobacter sakazakii via the consumption of infant-rich-inpolyphenols cocoa-milk-based beverages (CCX-M) treated with high-intensity pulsed electric fields (PEF) was evaluated. Monte Carlo simulation enabled the prediction of the variability in C. sakazakii load in beverages at the time of consumption to be estimated. Different scenarios (initial contamination levels; PEF treatment conditions; and time-temperature combinations of CCX-M beverages storage after treatment) were simulated. Cocoa addition and PEF treatment resulted in the most influential input factors to control bacterial final load. Cronobacter spp. exposure risk was reduced by a maximum of 100 times at 95% of iterations due to addition of cocoa at 5 g/100 mL, corresponding to scenario 3 (PEF: 15 kV/cm–3,000 μs; storage 120 h at 8 °C). Moreover, the probability of illness for a healthy population was reduced from 2.15 × 10-8, in the baseline scenario, to 4.78 × 10-10 due to cocoa addition and application of 15 kV/cm–3,000 μs PEF treatment.BISOSTAD projec

A new record of fossil wood of Vochysiaceae from the Upper Pleistocene (Arroyo Feliciano Formation), Argentina, South America

A new species of Qualeoxylon Suguio & Mussa is described from the Late Pleistocene Arroyo Feliciano Formation of the Gualeguay Basin on eastern Argentina. It is the second record of this genus in South America and was previously only known from Late Eocene to Early Miocene (late Pleistocene for some authors) on the Itaquaquecetuba Formation, São Paulo, Brazil. This new fossil species was recovered in the Consorcio Paso Sociedad fossil locality (Federal, Entre Ríos, Argentina). Wood anatomical features suggest a close relationship with Vochysiaceae and is an affi nity with the extant genus Qualea. This fossil wood is consistent with the warm and humid climate inferred for this region during the upper Pleistocene. The discovery of Qualeoxylon in Arroyo Feliciano Formation supports the hypothesis that the Vochysiaceae was more widespread in the past than today.Uma nova espécie de Qualeoxylon Suguio & Mussa é descrita para a Formação Arroyo Feliciano Bacia Gualeguay, Pleistoceno fi nal no leste da Argentina. É o segundo registro desse gênero na América do Sul, até agora só conhecido para o Eoceno Superior a Mioceno Inferior (Pleistoceno Superior para alguns autores) da Formação Itaquaquecetuba, São Paulo, Brasil. O espécime aqui estudado foi coletado na localidade fossilífera Consorcio Paso Sociedad (Federal, Entre Ríos, Argentina). As características anatômicas da madeira sugerem uma relação com a família Vochysiaceae e uma afi nidade com o atual gênero Qualea. Esta madeira fóssil condiz com o clima quente e úmido inferido para esta região durante o Pleistoceno fi nal. A descoberta de Qualeoxylon na Formação Arroyo Feliciano apoia a ideia de que a família Vochysiaceae foi mais amplamente distribuída no passado que nos dias atuais.Fil: Moya, Eliana Vanesa. Provincia de Entre Ríos. Centro de Investigaciones Científicas y Transferencia de Tecnología a la Producción. Universidad Autónoma de Entre Ríos. Centro de Investigaciones Científicas y Transferencia de Tecnología a la Producción. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Centro de Investigaciones Científicas y Transferencia de Tecnología a la Producción; ArgentinaFil: Brea, Mariana. Provincia de Entre Ríos. Centro de Investigaciones Científicas y Transferencia de Tecnología a la Producción. Universidad Autónoma de Entre Ríos. Centro de Investigaciones Científicas y Transferencia de Tecnología a la Producción. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Centro de Investigaciones Científicas y Transferencia de Tecnología a la Producción; Argentin

Pollination ecology and breeding system of two Calceolaria species in Chile

Indexación: Web of Science; Scielo.Many angiosperms are exclusively dependent on pollinators for its reproduction (Matallana et al.2010; Arroyo et al. 2006). However, pollinators sometimes could be erratic and variable in relation to the ecological context (e.g., plant community composition), especially in alpine zones where it is known that pollinators decline in abundance with the increment in altitude (Arroyo and Squeo1990; Totland 1994). The latest could be critical in specialized pollination system and more over when specialized flowering plants inhabit in sympatry, potentially sharing the specialized floral visitors. In this context, it is expected that plant species develop reproductive strategies to ensure reproduction and/or exhibit some differences in their pollination ecology.http://ref.scielo.org/dv2yv

Glueball masses in 2+1 dimensional SU(N) gauge theories with twisted boundary conditions

We analyze 2+1 dimensional Yang-Mills theory regularized on a lattice with twisted boundary conditions in the spatial directions. In previous work it was shown that the observables in the non-zero electric flux sectors obey the so-called $x$-scaling, i.e. depend only on the dimensionless variable $x\propto NL/b$ and the angle $\tilde\theta$ given by the parameters of the twist ($L$ being the length of the spatial torus and $b$ the inverse 't Hooft coupling). It is conjectured that this scaling is obeyed by all physical quantities. In this work we extend the previous analyses to the zero electric flux (glueball) sector. We study the mass of the lightest scalar glueball in two theories with different $N$ but matching $x$ and $\tilde\theta$ in a wide range of couplings from the perturbative small-volume regime to the non-perturbative one. We find that the results are consistent with the $x$-scaling hypothesis.Comment: 7 pages, contribution to the 32nd International Symposium on Lattice Field Theory, 23-28 June, 2014. Columbia University New York, NY; v2: references added, minor changes; PoS(LATTICE2014)05

A Description of Lobbying as Advocacy Public Relations

This study defines lobbying as advocacy public relations. Data were collected from self-administered surveys of 222 registered lobbyists in Oregon. This study provides insight into a specialized group of public relations practitioners

Non-Abelian Vortices on the Torus

We study periodic arrays of non-Abelian vortices in an $SU(N) \times U(1)$ gauge theory with $N_f$ flavors of fundamental matter multiplets. We carefully discuss the corresponding twisted boundary conditions on the torus and propose an ansatz to solve the first order Bogomolnyi equations which we find by looking to a bound of the energy. We solve the equations numerically and construct explicit vortex solutions

The Triassic-Jurassic boundary in the Andes of Argentina

The Arroyo Malo Formation at Alumbre Creek, on the northern bank of the Atuel River, west central Argentina, comprises a c. 300 m thick continuous marine succession across the Triassic-Jurassic System boundary, consisting of massive and laminated pelites indicative of a slope depositional environment. Late Triassic invertebrates, including ammonoids, nautiloids, bivalves, gastropods, brachiopods and corals are restricted to the lower 150 m. Beds between 125-135 m from the bottom yield Choristoceras cf. marshi Hauer, a species found in the Marshi/Crickmayi Zone of Europe and North America, together with loose fragments of Psiloceras cf. pressum Hillebrandt, coeval with the lower to middle part of the Hettangian Planorbis Zone. About 80 m higher are beds yielding Psiloceras cf. rectocostatum Hillebrandt, a species that gives name to an Andean biozone partially coeval with the Johnstoni and Plicatulum Subzones, upper Planorbis Zone. Other fossils recorded in the Rhaetian strata of this section are foraminifers, ostracods and plant remains identified as Zuberia cf. zuberi (Szaj.) Freng. and Clathropteris sp. The section was also sampled for conodonts and radiolarians, thus far with negative results. A palaeomagnetic study is underway.La Formazione Arroyo Malo ad Alumbre creek, sulla sponda settentrionale del fiume Atuel, Argentina centro-occidentale, comprende una successione marina continua spessa circa 300 m attraverso i! limite Triassico-Giurassico, ed e costituita da peliti massive e laminate indicative di un ambiente deposizionale di scarpata. Gli inverttfjrati del Ttiassico superiore, che includono ammonoidi, nautiloidi, bivalvi, gasteropodi, brachiopodi e coralli sono limitati ai primi 150 m. Gli strati fra i 125-135 m dalla base hanno dato Choristoceras cf. marshi Hauer, una specie trovata nella Zona a Marshi/Crickmayi di Europa e Nord America, insieme con frammenti sparsi di Psiloceras cf. pressum Hillebrandt, coeva con la parte mediana della Zona a Planorbis dell'Hettangiano. Circa. 80 m piu in alto ci sono strati contenenti Psiloceras cf. rectocostatum Hlllebrandt, una specie che da i! no me ad una biozona andina parzialmente coeva con le Sottózone a Johnstoni e Plicatulum, Zona a Planorbis superiore. Altri fossili documentati negli strati del Retico di questa sezione sono foraminiferi, ostracodi e resti di piante identificati come Zuberia cf. zuberi (Szaj.) Freng. e Clathropteris sp. La sezione e stata anche campionata per quanto riguarda conodonti e radiolari, finora con risultati negativi. Uno studio paleomagnetico e in corso.Fil: Riccardi, Alberto Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Departamento de Paleontología Invertebrados; ArgentinaFil: Damborenea, Susana Ester. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Departamento de Paleontología Invertebrados; ArgentinaFil: Manceñido, Miguel Oscar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Departamento de Paleontología Invertebrados; ArgentinaFil: Iglesia Llanos, Maria Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Geología. Instituto de Geofísica "Daniel Valencio"; Argentin