Comparative Studies on Antimicrobial and Antifungal Efficacy from Bixa Orellana L., Lantana Camara L., Stachytarpheta Jamaicensis (l.)vahl., Hyptis Suaveolens (l.) Poit.with Triclosan

The aim of the present study was to assess the Antimicrobial and Antifungal activities of the Phenolic leaf extracts of Bixa orellana L., Lantana camara L and Stachytarpheta jamaicensis (L.) Vahl. Hyptis suaveolens (L.) Piot. and the Triclosan, a chlorinated aromatic compound with antibacterial and antifungal properties used in common house hold and personal care products and to compare household and personal care products and to compare their effectiveness against 4 bacterial strains - 2 Gram Positive strains – Staphylococcus aurens and Bacillus substitis and 2 Gram negative strains – Escherischia coli and Pseudomonas fluorescens and 3 Fungi- Aspergillus niger, Aspergillus flavus and Mucor Sp., by Agar well diffusion Assay. The phenolic extracts of all the 4 plants showed Maximum (80-100%), Relative inhibition against Pseudomonas fluorescence, Moderate inhibition (30-70%) against Staphylococcus aurens and Bacillus substilis and least inhibition (30-47%) against Escherischia coli, while, the Antifungal efficacy of all the 4 Phenolic plant extracts were observed to be effective at the concentration ranging from 70-300 µg. The plant phenolic extracts for Antimicrobial and Antifungal properties were compared with Standard Triclosan, a chlorinated compound. Our studies showed that the phenolic components of plant origin for antibacterial activity were equivalent to Triclosan with the same concentration, while for antifungal activity slightly higher concentrations could be a better alternative and hence there could be a substitution for Triclosan by Plant Phenolic Extracts used in house hold and personal care products, in future days to come

Antifungal efficiency of plants aqueous extracts with potential for control of Cercospora oryzae miyake in rice

Rice (Oryza spp.) is the third most cultivated cereal crop in Benin, and major staple in the population’s daily diets. Its production, though is heavily impaired by fungal diseases, of which cercosporios accounts for about 60% yield loss. The objective of this study was to examine the efficacy of plant aqueous extracts for control of Cercospora oryzae diseases for improved rice production in Benin. We assessed the in vitro effectiveness of plant extracts on the sporulation and mycelial growth of C. oryzae, by evaluating the effectiveness of extracts on the foliar and panicular incidence, as well as the epidemic of C. oryzae in the field. A randomised complete block design arranged in split plots, was laid out with three spraying replications. The study combined phytiatric tools. Extracts from Lippia multiflora, Azadirachta indica and Eucalyptus camaldulensis at concentrations of 10 and 15% showed antifungal efficacy on Cercospora oryzae in vitro and in the field. Extracts of Cymbopogon citratus and Citrus limon did not exhibit antifungal efficacy against Cercospora oryzae

Formulation Of Lengkuas Rhizome (Alpinia Galanga L.) Extract's Gel AS Antifungal With Hidroxy Prophyl Methyl Cellulosae (Hpmc) And Carbopol Base

Infection of Malassezia furfur on skin can be caused by bad factor. One of the materials that can be used as an antifungal is Lengkuas rhizome extract (Alpinia galanga L.). This study had purpose to determine the antifungal activity and the effectiveness of gels Lengkuas rhizome extract on pathogenic fungi using maseration method with etanol 96%. Gel making is used HPMC and carbopol base. Testing activity of extract and testing the effectiveness of the gel by disc diffusion method (Kirby-Bauer test). Screening result showed that the extract contains triterpenoids, flavonoids and essential oil. Based on the test results against Malassezia furfur, antifungal activity of the extract in the gel increased compared to extracts without formulated into a gel. But the activity increasing was not significant based on statistical analysis with one-way ANOVA test obtained a significance of 0.234 (p> 0.05)

First Appearance of White Mould on Sunflower Caused by Sclerotinia Minor in the Republic of Macedonia

Sclerottnia spp. a very destructive fungus causing "white mould" became one of the biggest problems in sunflower breeding in the Republic of Macedo­nia in 2010. Field monitoring in the region of Bitola show very high infection of around 20-30%. Two types of symptoms where observed during the field mon­itoring. First symptoms were observed on the leaves of the Infected plants in the form of wilting, prior to flowering stage. The most characteristic symptoms were observed. at the lower part of the stem in the form of a stem cancer. Big variable sclerotia in size and shape were observed inside the stem. The appear­ence of white mycelium on the infected lower parts of the plant was often observed during the wet weather. Other infected plants showed different symp­toms. The stem was longer and thinner than in uninfected plants and the pit was very small around 9 cm. Sclerotia observed inside the stem were not big­ ger than 2.5 mm. In vitro investigations confirmed the presence of ascomycetes Sclerotinia sclerattorum (Lib.) de Bary and Sclerotinia minor Jagger, for the first time In the Republic of Macedonia. In vitra Investigation of antifungal abillty of Pseudomonas mediterranea Cattara et al., 2002 against S. sclerottorum and S.minor showed that it can be a possible antifungal agent against these ascomycetes

Antifungal efficacy of crude aqueous weed extracts against pathogen of cocoa black pod rot / Nor Amerulah Nor Mohamad, Suhaida Salleh and Hamzah Abdul Aziz

Black pod rot is the most economically important disease of cocoa in Malaysia which is mainly caused by a highly polyphagous Phytophthora species, called Phytophthora palmivora. The fungus could attack all parts of the cocoa plant organs and caused various diseases at any growth stage from seedling until the mature stages, especially during raining season. The application of synthetic fungicides has been widely recommended to manage the disease but their repeated use had led to other problems such as environmental, human health and development of fungicide resistance issues. This study isolated and identified Phytopththora isolate from a cocoa pod sample based on micro-morphological characters. Besides, the present investigation was undertaken to screen for the antifungal potency of different weed extracts against the Phytophthora pathogen using poisoned food technique. The fungal isolate was successfully recovered from pod tissues of clone PBC123 on 20% tomato juice agar culture (20T). Only one out of ten weed extracts tested showed a significant in vitro inhibitory effect towards mycelial growth of Phytophthora isolate, which was aqueous crude leaf extract of Solanum torvum (42.68%). This study indicated that the potential of weed extracts in the management of Phytophthora diseases, and may offer more natural, effective and economical control methods

Peel Diffusion and Antifungal Efficacy of Different Fungicides in Pear Fruit: Structure-Diffusion-Activity Relationships

Fungal pathogens can invade not only the fruit peel but also the outer part of the fruit mesocarp, limiting the efficacy of fungicides. In this study, the relationships between fungicide structure, diffusion capacity and in vivo efficacy were evaluated for the first time. The diffusion capacity from pear peel to mesocarp of 11 antifungal compounds, including p-aminobenzoic acid, carbendazim, difenoconazole, dipicolinic acid, flusilazole, gentamicin, kojic acid, prochloraz, quino-linic acid, thiophanate methyl and thiram was screened. The obtained results indicated that size and especially polarity were negatively correlated with the diffusion capacity. Although some antifungal compounds, such as prochloraz and carbendazim, were completely degraded after a few days in peel and mesocarp, other compounds, such as p-aminobenzoic acid and kojic acid, showed high stability. When applying the antifungal compounds at the EC50 concentrations, it was observed that the compounds with high diffusion capacity showed higher in vivo antifungal activity against Alternaria alternata than compounds with low diffusion capacity. In contrast, there was no relationship between stability and in vivo efficacy. Collectively, the obtained results indicated that the diffusion capacity plays an important role in the efficacy of fungicides for the control of pear fruit diseases. © 2022 by the authors. Licensee MDPI, Basel, Switzerland

Antifungal efficacy of chitosan nanoparticles against phytopathogenic fungi and inhibition of zearalenone production by Fusarium graminearum

Chitosan (COS) is a natural safe biopolymer that received great attention in agriculture, food, biomedical, pharmaceutical and environmental industries because their biocompatible, biodegradable, non-toxic and non-allergenic natures. The aims of the current study were to synthesize and characterize chitosan nanoparticles (COS-NPs), to evaluate their antifungal activity against phytopathogenic fungi and inhibition of zearalenone (ZEN) production by Fusarium graminearum. The results revealed that the deacetylation degree of COS was 86.9 0.44 %, the average of molar mass was 171.41 ± 0.29 g/mol, molecular weight was 244 ± 7 kDa and the concentration of free amino groups was 0.05 ± 0.019 mol L-1. COS-NPs showed the nanorod form with rough nature and particle size was around 180 nm. COS-NPs showed an excellent antifungal activity against Alternaria tenuis, Aspergillus niger, A. flavus, Baeuvaria bassiana, Fusarium graminearum, Fusarium oxysporum, Penicillium sp. and Sclerotium rolfsii in dose dependent manner. At a concentration of 800 ppm, it inhibits ZEN production by Fusarium graminearum. It could be concluded that COS-NPs are promise candidate as safe antifungal capable for the prevention of ZEN production.Chitosan (COS) is a natural safe biopolymer that received great attention in agriculture, food, biomedical, pharmaceutical and environmental industries because their biocompatible, biodegradable, non-toxic and non-allergenic natures. The aims of the current study were to synthesize and characterize chitosan nanoparticles (COS-NPs), to evaluate their antifungal activity against phytopathogenic fungi and inhibition of zearalenone (ZEN) production by Fusarium graminearum. The results revealed that the deacetylation degree of COS was 86.9 0.44 %, the average of molar mass was 171.41 ± 0.29 g/mol, molecular weight was 244 ± 7 kDa and the concentration of free amino groups was 0.05 ± 0.019 mol L-1. COS-NPs showed the nanorod form with rough nature and particle size was around 180 nm. COS-NPs showed an excellent antifungal activity against Alternaria tenuis, Aspergillus niger, A. flavus, Baeuvaria bassiana, Fusarium graminearum, Fusarium oxysporum, Penicillium sp. and Sclerotium rolfsii in dose dependent manner. At a concentration of 800 ppm, it inhibits ZEN production by Fusarium graminearum. It could be concluded that COS-NPs are promise candidate as safe antifungal capable for the prevention of ZEN production