Effective adhesion strength of specifically bound vesicles

A theoretical approach has been undertaken in order to model the thermodynamic equilibrium of a vesicle adhering to a flat substrate. The vesicle is treated in a canonical description with a fixed number of sites. A finite number of these sites are occupied by mobile ligands that are capable of interacting with a discrete number of receptors immobilized on the substrate. Explicit consideration of the bending energy of the vesicle shape has shown that the problem of the vesicle shape can be decoupled from the determination of the optimum allocation of ligands over the vesicle. The allocation of bound and free ligands in the vesicle could be determined as a function of the size of the contact zone, the ligand-receptor binding strength and the concentration of the system constituents. Several approximate solutions for different regions of system parameters are determined and in particular, the distinction between receptor-dominated equilibria and ligand-dominated equilibria is found to be important. The crossover between these two types of solutions is found to occur at a critical size of the contact zone. The presented approach enables the calculation of the effective adhesion strength of the vesicle and thus permits meaningful comparisons with relevant experiments as well as connecting the presented model with the proven success of the continuum approach for modeling the shapes of adhering vesicles. The behavior of the effective adhesion strength is analyzed in detail and several approximate expressions for it are given.Comment: 19 pages, 6 figures. To appear in Phys. Rev.

Low Cost, Adhesion Strength Based Cell Sorter

In many in vitro experiments, primary cells are harvested from an animal species to undergo experimental manipulation and subsequent analysis. Cell sorters are a luxury to have after cell harvesting to ensure pure populations of cells. Recently, it was discovered that different cell types adhere to cell culture dishes at different strengths. This observation was utilized in the invention of a cell sorting system that sorts cells based on this adhesion strength difference. The resulting system is marketable at under $2000, compared to$50000 plus for current commercially available systems, and is the first commercially-oriented cell adhesion strength based cell sorter. The system allows for use of tissue culture flasks, a tool that any researcher using cells will be very familiar with, that have been customized with a removable bottom. After cells are adhered, the bottom is removed and placed into the cell sorting system and sealed with a removable sealing putty. Flow is imparted to the cells, generating a shear force over the surface of the cells, lifting the cells and depositing them into a centrifuge tube. Prior experiments have shown this system to have a cell purity and cell viability greater than currently available solutions without the use of expensive antibodies and using equipment available at a fraction of the cost.https://scholarscompass.vcu.edu/capstone/1145/thumbnail.jp

Role of cell deformability in the two-dimensional melting of biological tissues

The size and shape of a large variety of polymeric particles, including biological cells, star polymers, dendrimes, and microgels, depend on the applied stresses as the particles are extremely soft. In high-density suspensions these particles deform as stressed by their neighbors, which implies that the interparticle interaction becomes of many-body type. Investigating a two-dimensional model of cell tissue, where the single particle shear modulus is related to the cell adhesion strength, here we show that the particle deformability affects the melting scenario. On increasing the temperature, stiff particles undergo a first-order solid/liquid transition, while soft ones undergo a continuous solid/hexatic transition followed by a discontinuous hexatic/liquid transition. At zero temperature the melting transition driven by the decrease of the adhesion strength occurs through two continuous transitions as in the Kosterlitz, Thouless, Halperin, Nelson, and Young scenario. Thus, there is a range of adhesion strength values where the hexatic phase is stable at zero temperature, which suggests that the intermediate phase of the epithelial-to-mesenchymal transition could be hexatic type

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The objective of this study was to evaluate the surface coating performance using water base, solvent base and powder coating on medium density fiberboard. Samples were coated using water-base, solvent-base and powder coating. Coating performance was performed by using adhesion strength, surface coating hardness, layer thickness and rapid deformation test. The results were analyzed with two different statistical methods. Adhesion strength, layer thickness and surface hardness were determined by using ANOVA analysis, while results of rapid deformation test were analyzed by using Kruskal-Wallis method. According to these results, coating type was effective factor on the adhesion strength, surface coating hardness, layer thickness and rapid deformation test. Water-based painting application for adhesion strength and impact deformation resistance was higher than solvent-based coating. In the powder coating application, although surface coating hardness was higher than solvent and water base coating application, rapid deformation results were lower than solvent and water base coating applications. Adequacy of models was performed R-square (R²) and Adjusted R-square (Adj-R2) values. R2 values of adhesion strength, layer hardness and film layer thickness were 93.60 %, 95.33 % and 73.90 %, respectively. Adj-R2 values of adhesion strength, layer hardness and film layer thickness were 93.45 %, 95.23 % and 73.30 %, respectively

Preliminary evaluation of adhesion strength measurement devices for ceramic/titanium matrix composite bonds

The adhesive bond between ceramic cement and a titanium matrix composite substrate to be used in the National Aerospace Plane program is evaluated. Two commercially available adhesion testers, the Sebastian Adherence Tester and the CSEM REVETEST Scratch Tester, are evaluated to determine their suitability for quantitatively measuring adhesion strength. Various thicknesses of cements are applied to several substrates, and bond strengths are determined with both testers. The Sabastian Adherence Tester has provided limited data due to an interference from the sample mounting procedure, and has been shown to be incapable of distinguishing adhesion strength from tensile and shear properties of the cement itself. The data from the scratch tester has been found to be difficult to interpret due to the porosity and hardness of the cement. Recommendations are proposed for a more reliable adhesion test method

Finishing characteristics of heat treated and compressed rubberwood.

The finishing characteristics of heat-treated and compressed Rubberwood were studied. It was found that heat-treated samples had significantly lower finish adhesion strength compared to the conventionally kiln-dried Rubberwood samples, which were used as control specimens. Further, compression of the specimens had also reduced its surface roughness, with an adverse effect on the finish adhesion strength. Inevitably, the use of heat treated and compressed wood in furniture may be suitable for applications, where lower finish adhesion strength could be tolerated at the expense of better finish film quality and process economics, especially in the manufacture of outdoor wooden furniture

Utilizing swelling force to decrease the ice adhesion strength

The phase transformation that occurs during water freezing process is accompanied by volume expansion and the release of latent heat. The swelling force generated by this phase transformation can have a harmful impact on structural safety and integrity, as it can lead to bursting in roads, water pipes and reservoir dams. So, why not effectively adopt the swelling force as the active de-icing power to diminish the stability of the contact interface. This paper proposes a new method to remove this accumulated ice by using polymethyl methacrylate (pmma) and 6061 aluminum alloy with pits as substrate materials. Pits were filled with solutions of different freezing points; owing to the different freezing point between the pit solution and water, their phase transformations occurred at different time, where the solutions in the pit would freeze more slowly than the surface water. The generated phase swelling force directly acted on the contact interface and decreased the stability of the interface to decrease the ice adhesion strength. The experimental results showed that the ice adhesion strength was obviously affected and reduced by the swelling force in contrast to the ice adhesion strength on the smooth sample, and the reduction in ice adhesion strength changed depending on the filling solution. Compared to the ice adhesion strength of the specimen without pits, the frozen ice was completely separated from the ice-pmma interface owing to the water filling the pit. The ice adhesion strength on the surface of the aluminum alloy sample filled with 10% ethanol solution was reduced by 81.42%. Utilizing the phase swelling force to reduce the adhesion strength enhances the active de-icing ability of the material, providing a novel method for developing new anti-icing methods

Effects of impregnation with boron compounds on the surface adhesion strength of varnishes used woods

The aim of this study was to determine the effects of impregnation with boron compounds on the surface adhesion strength of varnishes used woods. For this purpose, the test specimens prepared from Oriental beech (Fagus orientalis Lipsky) and European oak (Quercus petreae (Matt.) Liebl.) which met the requirements of ASTM D 358 were impregnated according to ASTM D 1413 with boric acid (Ba) and borax (Bx) by vacuum technique. After impregnation, surfaces were coated with cellulosic, synthetic, polyurathane, water-based, acrylic and acid hardening varnishes in accordance with ASTM D 3023 standards. According to ASTM D 4541 standards, the surface adhesion strength of specimens after varnishing process was determined. Considering the interaction of wood type, impregnation material and varnish type, adhesion strength values were found the highest in Oriental beech + boric acid + synthetic varnish (6.240 Mpa) and the lowest values in European oak + borax + cellulosic varnish (2.080 Mpa). Consequently, impregnation of wood material with boron compounds (boric acid: 3.677 Mpa and borax : 3.732 Mpa) showed increasing impact on the surface adhesion strength of cellulosic, synthetic, polyurathane, water-based, acrylic and acid hardening varnishes.Key words: Adhesion strength, impregnation, boric acid, borax, varnishes, finishing, wood

Fundamentals of the adhesion of physical vapor deposited ZnMg-Zn bilayer coatings to steel substrates

In the present study, ZnMg-Zn bilayer coatings with different Mg concentrations and layer thicknesses are deposited on steel substrates by a thermal evaporation process. Thermodynamic calculations reveal that the work of adhesion at the ZnMg/Zn interface (~1.6 J/m2) is lower than that at the Zn/Steel interface (~3 J/m2). This indicates that the ZnMg/Zn interface is inherently weaker than the interface between Zn and steel substrate. The interfacial adhesion strength quantified by the scratch test shows that the adhesion strength at the ZnMg/Zn interface decreases with increasing the Mg content and reaches 66 MPa at 16.5 wt% Mg. It is found that the presence of interfacial defects largely decreases the adhesion strength compared to a defect-free coating. Meanwhile, it is also concluded that the interfacial adhesion strength at the ZnMg/Zn interface does not depend on the thickness of Zn interlayer. The results of the present investigation show that the interfacial adhesion strength is not the only governing parameter for the adhesion performance of the ZnMg-Zn bilayer coatings in forming process, but the thickness of the layers as well as interfacial defect density also play important roles in the adhesion performance