762 research outputs found
Collective motion of cells: from experiments to models
Swarming or collective motion of living entities is one of the most common
and spectacular manifestations of living systems having been extensively
studied in recent years. A number of general principles have been established.
The interactions at the level of cells are quite different from those among
individual animals therefore the study of collective motion of cells is likely
to reveal some specific important features which are overviewed in this paper.
In addition to presenting the most appealing results from the quickly growing
related literature we also deliver a critical discussion of the emerging
picture and summarize our present understanding of collective motion at the
cellular level. Collective motion of cells plays an essential role in a number
of experimental and real-life situations. In most cases the coordinated motion
is a helpful aspect of the given phenomenon and results in making a related
process more efficient (e.g., embryogenesis or wound healing), while in the
case of tumor cell invasion it appears to speed up the progression of the
disease. In these mechanisms cells both have to be motile and adhere to one
another, the adherence feature being the most specific to this sort of
collective behavior. One of the central aims of this review is both presenting
the related experimental observations and treating them in the light of a few
basic computational models so as to make an interpretation of the phenomena at
a quantitative level as well.Comment: 24 pages, 25 figures, 13 reference video link
Polarity, cell division, and out-of-equilibrium dynamics control the growth of epithelial structures
The growth of a well-formed epithelial structure is governed by mechanical constraints, cellular apico-basal polarity, and spatially controlled cell division. Here we compared the predictions of a mathematical model of epithelial growth with the morphological analysis of 3D epithelial structures. In both in vitro cyst models and in developing epithelial structures in vivo, epithelial growth could take place close to or far from mechanical equilibrium, and was determined by the hierarchy of time-scales of cell division, cell-cell rearrangements, and lumen dynamics. Equilibrium properties could be inferred by the analysis of cell-cell contact topologies, and the nonequilibrium phenotype was altered by inhibiting ROCK activity. The occurrence of an aberrant multilumen phenotype was linked to fast nonequilibrium growth, even when geometric control of cell division was correctly enforced. We predicted and verified experimentally that slowing down cell division partially rescued a multilumen phenotype induced by altered polarity. These results improve our understanding of the development of epithelial organs and, ultimately, of carcinogenesi
Cellular Potts modeling of complex multicellular behaviors in tissue morphogenesis
Mathematical modeling is an essential approach for the understanding of complex multicellular behaviors in tissue morphogenesis. Here, we review the cellular Potts model (CPM; also known as the Glazier-Graner-Hogeweg model), an effective computational modeling framework. We discuss its usability for modeling complex developmental phenomena by examining four fundamental examples of tissue morphogenesis: (i) cell sorting, (ii) cyst formation, (iii) tube morphogenesis in kidney development, and (iv) blood vessel formation. The review provides an introduction for biologists for starting simulation analysis using the CPM framework
Computational modeling of angiogenesis: towards a multi-scale understanding of cell-cell and cell-matrix interactions
Combined with in vitro and in vivo experiments, mathematical and com-
putational modeling are key to unraveling how mechanical and chemical signaling
by endothelial cells coordinates their organization into capillary-like tubes. While
in vitro and in vivo experiments can unveil the effects of for example environmental
changes or gene knockouts, computational models provide a way to formalize and
understand the mechanisms underlying these observations. This chapter reviews re-
cent computational approaches to model angiogenesis, and discusses the insights
they provide in the mechanisms of angiogenesis.
We introduce a new cell-based computational model of an in vitro assay of angio-
genic sprouting from endothelial monolayers in fibrin matrices. Endothelial cells
are modeled by the Cellular Potts Model, combined with continuum descriptions
to model haptotaxis and proteolysis of the extracellular matrix. The computational
model demonstrates how a variety of cellular structural properties and behaviors
determine the dynamics of tube formation. We aim to extend this model to a multi-scale model in the sense that cells, extracellular matrix and cell-regulation are de-
scribed at different levels of detail and feedback on each other. Finally we discuss
how computational modeling, combined with in vitro and in vivo modeling steers
experiments, and how it generates new experimental hypotheses and insights on the
mechanics of angiogenesis
A cellular Potts model analyzing differentiated cell behavior during in vivo vascularization of a hypoxic tissue
Angiogenesis, the formation of new blood vessel networks from existing capillary or post-capillary venules, is an intrinsically multiscale process occurring in several physio-pathological conditions. In particular, hypoxic tissue cells activate downstream cascades culminating in the secretion of a wide range of angiogenic factors, including VEGF isoforms. Such diffusive chemicals activate the endothelial cells (ECs) forming the external walls of the nearby vessels that chemotactically migrate toward the hypoxic areas of the tissue as multicellular sprouts. A functional network eventually emerges by further branching and anastomosis processes. We here propose a CPM-based approach reproducing selected features of the angiogenic progression necessary for the reoxygenation of a hypoxic tissue. Our model is able to span the different scale involved in the angiogenic progression as it incorporates reaction-diffusion equations for the description of the evolution of microenvironmental variables in a discrete mesoscopic cellular Potts model (CPM) that reproduces the dynamics of the vascular cells. A key feature of this work is the explicit phenotypic differentiation of the ECs themselves, distinguished in quiescent, stalk and tip. The simulation results allow identifying a set of key mechanisms underlying tissue vascularization. Further, we provide evidence that the nascent pattern is characterized by precise topological properties. Finally, we link abnormal sprouting angiogenesis with alteration in selected cell behavior
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