Mehitamata õhusõiduki rakendamine põllukultuuride saagikuse ja maa harimisviiside tuvastamisel

A Thesis for applying for the degree of Doctor of Philosophy in Environmental Protection.Väitekiri filosoofiadoktori kraadi taotlemiseks keskkonnakaitse erialal.This thesis aims to examine how machine learning (ML) technologies have aided significant advancements in image analysis in the area of precision agriculture. These multimodal computing technologies extend the use of machine learning to a broader spectrum of data collecting and selection for the advancement of agricultural practices (Nawar et al., 2017) These techniques will assist complicated cropping systems with more informed decisions with less human intervention, and provide a scalable framework for incorporating expert knowledge of the PA system. (Chlingaryan et al., 2018). Complexity, on the other hand, can be seen as a disadvantage in crop trials, as machine learning models require training/testing databases, limited areas with insignificant sampling sizes, time and space-specificity, and environmental factor interventions, all of which complicate parameter selection and make using a single empirical model for an entire region impractical. During the early stages of writing this thesis, we used a relatively traditional machine learning method to address the regression problem of crop yield and biomass prediction [(i.e., random forest regression (RFR), support vector regression (SVR), and artificial neural network (ANN)] to predicted dry matter (DM) yields of red clover. It obtained favourable results, however, the choosing of hyperparameters, the lengthy algorithms selection process, data cleaning, and redundant collinearity issues significantly limited the way of the machine learning application. We will further discuss the recent trend of automated machine learning (AutoML) that has been driving further significant technological innovation in the application of artificial intelligence from its automated algorithm selection and hyperparameter optimization of the deployable pipeline model for unravelling substance problems. However, a present knowledge gap exists in the integration of machine learning (ML) technology with unmanned aerial systems (UAS) and hyperspectral-based imaging data categorization and regression applications. In this thesis, we explored a state-of-the-art (SOTA) and entirely open-source AutoML framework, Auto-sklearn, which was built on one of the most frequently used machine learning systems, Scikit-learn. It was integrated with two unique AutoML visualization tools to examine the recognition and acceptance of multispectral vegetation indices (VI) data collected from UAS and hyperspectral narrow-band VIs across a varied spectrum of agricultural management practices (AMP). These procedures incorporate soil tillage method (STM), cultivation method (CM), and manure application (MA), and are classified as four-crop combination fields (i.e., red clover-grass mixture, spring wheat, pea-oat mixture, and spring barley). Additionally, they have not been thoroughly evaluated and lack characteristics that are accessible in agriculture remote sensing applications. This thesis further explores the existing gaps in the knowledge base for several critical crop categories and cultivation management methods referring to biomass and yield analysis, as well as to gain a better understanding of the potential for remotely sensed solutions to field-based and multifunctional platforms to meet precision agriculture demands. To overcome these knowledge gaps, this research introduces a rapid, non-destructive, and low-cost framework for field-based biomass and grain yield modelling, as well as the identification of agricultural management practices. The results may aid agronomists and farmers in establishing more accurate agricultural methods and in monitoring environmental conditions more effectively.Doktoritöö eesmärk oli uurida, kuidas masinõppe (MÕ) tehnoloogiad võimaldavad edusamme täppispõllumajanduse valdkonna pildianalüüsis. Multimodaalsed arvutustehnoloogiad laiendavad masinõppe kasutamist põllumajanduses andmete kogumisel ja valimisel (Nawar et al., 2017). Selline täpsemal informatsioonil põhinev tehnoloogia võimaldab keerukate viljelussüsteemide puhul teha otsuseid inimese vähema sekkumisega, ja loob skaleeritava raamistiku täppispõllumajanduse jaoks (Chlingaryan et al., 2018). Põllukultuuride katsete korral on komplekssete masinõppemudelite kasutamine keerukas, sest alad on piiratud ning valimi suurus ei ole piisav; vaja on testandmebaase, kindlaid aja- ja ruumitingimusi ning keskkonnategureid. See komplitseerib parameetrite valikut ning muudab ebapraktiliseks ühe empiirilise mudeli kasutamise terves piirkonnas. Siinse uurimuse algetapis rakendati suhteliselt traditsioonilist masinõppemeetodit, et lahendada saagikuse ja biomassi prognoosimise regressiooniprobleem (otsustusmetsa regression, tugivektori regressioon ja tehisnärvivõrk) punase ristiku prognoositava kuivaine saagikuse suhtes. Saadi sobivaid tulemusi, kuid hüperparameetrite valimine, pikk algoritmide valimisprotsess, andmete puhastamine ja kollineaarsusprobleemid takistasid masinõpet oluliselt. Automatiseeritud masinõppe (AMÕ) uusimate suundumustena rakendatakse tehisintellekti, et lahendada põhiprobleemid automatiseeritud algoritmi valiku ja rakendatava pipeline-mudeli hüperparameetrite optimeerimise abil. Seni napib teadmisi MÕ tehnoloogia integreerimiseks mehitamata õhusõidukite ning hüperspektripõhiste pildiandmete kategoriseerimise ja regressioonirakendustega. Väitekirjas uuriti nüüdisaegset ja avatud lähtekoodiga AMÕ tehnoloogiat Auto-sklearn, mis on ühe enimkasutatava masinõppesüsteemi Scikit-learn edasiarendus. Süsteemiga liideti kaks unikaalset AMÕ visualiseerimisrakendust, et uurida mehitamata õhusõidukiga kogutud andmete multispektraalsete taimkatteindeksite ja hüperspektraalsete kitsaribaandmete taimkatteindeksite tuvastamist ja rakendamist põllumajanduses. Neid võtteid kasutatakse mullaharimisel, kultiveerimisel ja sõnnikuga väetamisel nelja kultuuriga põldudel (punase ristiku rohusegu, suvinisu, herne-kaera segu, suvioder). Neid ei ole põhjalikult hinnatud, samuti ei hõlma need omadusi, mida kasutatatakse põllumajanduses kaugseire rakendustes. Uurimus käsitleb biomassi ja saagikuse seni uurimata analüüsivõimalusi oluliste põllukultuuride ja viljelusmeetodite näitel. Hinnatakse ka kaugseirelahenduste potentsiaali põllupõhiste ja multifunktsionaalsete platvormide kasutamisel täppispõllumajanduses. Uurimus tutvustab kiiret, keskkonna suhtes kahjutut ja mõõduka hinnaga tehnoloogiat põllupõhise biomassi ja teraviljasaagi modelleerimiseks, et leida sobiv viljelusviis. Töö tulemused võimaldavad põllumajandustootjatel ja agronoomidel tõhusamalt valida põllundustehnoloogiaid ning arvestada täpsemalt keskkonnatingimustega.Publication of this thesis is supported by the Estonian University of Life Scieces and by the Doctoral School of Earth Sciences and Ecology created under the auspices of the European Social Fund

Deep learning in plant phenological research: A systematic literature review

Climate change represents one of the most critical threats to biodiversity with far-reaching consequences for species interactions, the functioning of ecosystems, or the assembly of biotic communities. Plant phenology research has gained increasing attention as the timing of periodic events in plants is strongly affected by seasonal and interannual climate variation. Recent technological development allowed us to gather invaluable data at a variety of spatial and ecological scales. The feasibility of phenological monitoring today and in the future depends heavily on developing tools capable of efficiently analyzing these enormous amounts of data. Deep Neural Networks learn representations from data with impressive accuracy and lead to significant breakthroughs in, e.g., image processing. This article is the first systematic literature review aiming to thoroughly analyze all primary studies on deep learning approaches in plant phenology research. In a multi-stage process, we selected 24 peer-reviewed studies published in the last five years (2016–2021). After carefully analyzing these studies, we describe the applied methods categorized according to the studied phenological stages, vegetation type, spatial scale, data acquisition- and deep learning methods. Furthermore, we identify and discuss research trends and highlight promising future directions. We present a systematic overview of previously applied methods on different tasks that can guide this emerging complex research field

Artificial Neural Networks in Agriculture

Modern agriculture needs to have high production efficiency combined with a high quality of obtained products. This applies to both crop and livestock production. To meet these requirements, advanced methods of data analysis are more and more frequently used, including those derived from artificial intelligence methods. Artificial neural networks (ANNs) are one of the most popular tools of this kind. They are widely used in solving various classification and prediction tasks, for some time also in the broadly defined field of agriculture. They can form part of precision farming and decision support systems. Artificial neural networks can replace the classical methods of modelling many issues, and are one of the main alternatives to classical mathematical models. The spectrum of applications of artificial neural networks is very wide. For a long time now, researchers from all over the world have been using these tools to support agricultural production, making it more efficient and providing the highest-quality products possible

Precision Agriculture Technology for Crop Farming

This book provides a review of precision agriculture technology development, followed by a presentation of the state-of-the-art and future requirements of precision agriculture technology. It presents different styles of precision agriculture technologies suitable for large scale mechanized farming; highly automated community-based mechanized production; and fully mechanized farming practices commonly seen in emerging economic regions. The book emphasizes the introduction of core technical features of sensing, data processing and interpretation technologies, crop modeling and production control theory, intelligent machinery and field robots for precision agriculture production

Computer processing of peach tree decline data

There are no author-identified significant results in this report

by integrating deep learning, mechanistic model and field observations

학위논문(박사) -- 서울대학교대학원 : 농업생명과학대학 협동과정 농림기상학, 2022. 8. Youngryel Ryu.Rice (Oryza sativa) is a vital cereal crop that feeds more than 50% of the world population. However, the traditional anaerobic management leads rice production to consume ~40% of the irrigation water and emit ~10% of the global anthropogenic methane. A new paradigm for sustainable rice farming is urgently required amid challenges from increasing food demand, water scarcity, and reducing greenhouse gases emissions. Rice plants transpire considerable water overnight. Saving nighttime water loss is desirable but first need to understand the underlying mechanism of nocturnal stomatal opening. Apart from the night, optimizing daytime management is pivotal for designing an environmentally sustainable rice farming system. In a long-term strategy, detailed and reliable crop type map is compulsory to upscale new leaf level findings and site level methods to regional or global scale. Therefore, in this dissertation, we improved mechanistic understanding of nocturnal stomatal conductance in rice plants (Chapter II); provided an interdisciplinary and heuristic approach for designing an environmentally sustainable rice farming system with a case study in South Korea (Chapter III); and developed a new crop type referencing method by mining off-the-shelf Google Street View images to map crop types (Chapter IV). In chapter II, we proposed a “coordinated leaf trait” hypothesis to explain the ecological mechanism of nocturnal stomatal conductance (gsn) in rice. We conducted an open-field experiment by applying drought, nutrient deficiency, and the combined drought-nutrient deficiency stress. We found that gsn was neither strongly reduced by drought nor consistently increased by nutrient deficiency. With abiotic stress as a random effect, gsn was strongly positively correlated with nocturnal respiration (Rn). Notably, gsn primed early morning photosynthesis, as follows: Rn (↑) → gsn (↑) → gsd (daytime stomatal conductance) (↑) → A (assimilation) (↑). This photosynthesis priming effect diminished after mid-morning. Leaves were cooled by gsn as follows: gsn (↑) → E (transpiration) (↑) → Tleaf (leaf temperature) (↓). However, our results clearly suggest that evaporative cooling did not reduce Rn cost. Our results indicate that gsn is more closely related to carbon respiration and assimilation than water and nutrient availability, and that leaf trait coordination (Rn − gsn − gsd − A) is likely the primary mechanism controlling gsn. In chapter III, we aimed to increase current crop yield, reduce irrigation water consumption, and tackle the dilemma to simultaneously reducing CH4 and N2O emissions in a flooded rice production system. By proposing a heuristic and holistic method, we optimized farm management beyond previous most emphasized irrigation regimes while also exploring niches from other pivotal options regarding sowing window, fertilization rate, tillage depth, and their interactions. Specifically, we calibrated and validated the process-based DNDC model with five years of eddy covariance observations. The DNDC model later was integrated with the non-dominated sorting genetic algorithm (NSGA-III) to solve the multi-objective optimization problem. We found that the optimized management would maintain or even increase current crop yield to its potential (~10 t/ha) while reducing more than 50% irrigation demand and GHGs (CH4 & N2O) emissions. Our results indicate that earlier sowing window and improvements on irrigation practice together would be pivotal to maximizing crop yield while sustaining environmental benefits. We found that the optimal fraction of non-flooded days was around 54% of growing season length and its optimal temporal distributions were primarily in vegetative stages. Our study shows that the present farm yield (8.3-8.9 t/ha) in study site not only has not achieved its potential level but also comes at a great environmental cost to water resources (604-810 mm/yr) and GHGs emissions (CH4: 186-220 kg C/ha/yr; N2O: 0.3-1.6 kg C/ha/yr). Furthermore, this simple method could further be applied to evaluate the environmental sustainability of a farming system under various climate and local conditions and to guide policymakers and farming practices with comprehensive solutions. In chapter IV, we apply a convolutional neural network (CNN) model to explore the efficacy of automatic ground truthing via Google Street View (GSV) images in two distinct farming regions: Illinois and the Central Valley in California. Ground reference data are an essential prerequisite for supervised crop mapping. The lack of a low-cost and efficient ground referencing method results in pervasively limited reference data and hinders crop classification. In this study, we demonstrate the feasibility and reliability of our new ground referencing technique by performing pixel-based crop mapping at the state level using the cloud-based Google Earth Engine platform. The mapping results are evaluated using the United States Department of Agriculture (USDA) crop data layer (CDL) products. From ~130,000 GSV images, the CNN model identified ~9,400 target crop images. These images are well classified into crop types, including alfalfa, almond, corn, cotton, grape, rice, soybean, and pistachio. The overall GSV image classification accuracy is 92% for the Central Valley and 97% for Illinois. Subsequently, we shifted the image geographical coordinates 2–3 times in a certain direction to produce 31,829 crop reference points: 17,358 in Illinois, and 14,471 in the Central Valley. Evaluation of the mapping results with CDL products revealed satisfactory coherence. GSV-derived mapping results capture the general pattern of crop type distributions for 2011–2019. The overall agreement between CDL products and our mapping results is indicated by R2 values of 0.44–0.99 for the Central Valley and 0.81–0.98 for Illinois. To show the applicational value of the proposed method in other countries, we further mapped rice paddy (2014–2018) in South Korea which yielded fairly well outcomes (R2=0.91). These results indicate that GSV images used with a deep learning model offer an efficient and cost-effective alternative method for ground referencing, in many regions of the world.쌀(오리자 사티바)은 세계 인구의 50% 이상을 먹여 살리는 중요한 곡물 작물이다. 그러나 전통적인 혐기성 관리는 쌀 생산으로 관개수의 40%를 소비하고 전 세계 인공 메탄의 10%를 배출한다. 식량 수요 증가, 물 부족, 온실가스 배출 감소 등의 과제 속에서 지속 가능한 벼농사를 위한 새로운 패러다임이 시급하다. 벼는 하룻밤 사이에 상당한 양의 물을 내뿜는다. 야간 수분 손실을 줄이는 것은 바람직하지만, 먼저 야간 기공 개방의 기본 메커니즘을 이해할 필요가 있다. 야간과 별도로 주간 경영의 최적화는 환경적으로 지속 가능한 벼농사 시스템을 설계하는 데 매우 중요하다. 장기 전략에서, 새로운 잎 수준 발견과 현장 수준 방법을 지역적 또는 전역적 규모로 상향 조정하려면 상세하고 신뢰할 수 있는 작물 유형 맵이 필수적이다. 따라서, 본 논문에서 우리는 벼농사의 야간 기공 전도도에 대한 기계적 이해를 향상시켰다(제2장). 환경적으로 지속 가능한 벼농사 시스템을 설계하기 위한 학제 간 및 휴리스틱 접근법 제공(제3장). 그리고 새로운 작물 유형 참조 방법을 개발했다. 기성품인 Google Street View 이미지를 마이닝하여 자르기 유형을 매핑합니다. 2장에서 우리는 벼의 야행성 기공 전도도(gsn)의 생태학적 메커니즘을 설명하기 위해 "협동된 잎 형질" 가설을 제안했습니다. 가뭄, 영양 결핍 및 가뭄-영양소 결핍 복합 스트레스를 적용하여 노지 실험을 수행했습니다. 우리는 gsn이 가뭄에 의해 크게 감소하지도 않고 영양 결핍에 의해 지속적으로 증가하지도 않는다는 것을 발견했습니다. 무생물적 스트레스를 무작위 효과로 사용하여 gsn은 야간 호흡(Rn)과 강한 양의 상관관계를 보였습니다. 특히, gsn은 Rn(↑) → gsn(↑) → gsd(주간 기공 전도도)(↑) → A(동화)(↑)와 같이 이른 아침 광합성을 프라이밍했습니다. 이 광합성 프라이밍 효과는 오전 중반 이후에 감소했습니다. 잎은 gsn에 의해 다음과 같이 냉각되었습니다: gsn(↑) → E(증산)(↑) → Tleaf(잎 온도)(↓). 그러나 우리의 결과는 증발 냉각이 Rn 비용을 감소시키지 않았다는 것을 분명히 시사합니다. 우리의 결과는 gsn이 물 및 영양소 가용성보다 탄소 호흡 및 동화와 더 밀접하게 관련되어 있으며 잎 형질 조정(Rn - gsn - gsd - A)이 gsn을 제어하는 주요 메커니즘일 가능성이 있음을 나타냅니다. 제3장에서 우리는 현재의 작물 수확량을 늘리고 관개 용수 소비를 줄이며 침수된 쌀 생산 시스템에서 CH4와 N2O 배출량을 동시에 줄이는 딜레마를 해결하는 것을 목표로 했다. 휴리스틱하고 전체론적 방법을 제안함으로써, 우리는 이전에 가장 강조되었던 관개 체제를 넘어 농장 관리를 최적화함과 동시에 파종 창, 수정률, 경작 깊이 및 이들의 상호 작용과 관련된 다른 중추적 옵션의 틈새를 탐색했다. 구체적으로, 우리는 5년간의 와류 공분산 관찰로 프로세스 기반 DNDC 모델을 교정하고 검증했다. DNDC 모델은 나중에 다중 객관적 최적화 문제를 해결하기 위해 비지배적 정렬 유전 알고리듬(NSGA-III)과 통합되었다. 우리는 최적화된 관리를 통해 50% 이상의 관개 수요와 GHG(CH4 & N2O) 배출량을 줄이면서 현재 농작물 수확량을 잠재력(~10t/ha)까지 유지하거나 증가시킬 수 있다는 것을 발견했습니다. 우리의 결과는 더 이른 파종 기간과 관개 관개 관행의 개선이 환경적 이익을 유지하면서 농작물 수확량을 최대화하는 데 중추적일 것이라는 것을 보여준다. 우리는 홍수 없는 날의 최적 부분이 성장기 길이의 약 54%였고 최적의 시간 분포는 주로 식물 단계에 있다는 것을 발견했다. 우리의 연구는 연구 현장의 현재 농장 수확량(8.3-8.9 t/ha)이 잠재적 수준을 달성했을 뿐만 아니라 수자원(604-810 mm/yr)과 GHGs 배출(CH4: 186-220 kg C/ha/yr; N2O: 0.3-1.6 kg C/ha/yr)에 막대한 환경 비용을 초래한다는 것을 보여준다. 또한, 이 간단한 방법은 다양한 기후 및 지역 조건 하에서 농업 시스템의 환경 지속 가능성을 평가하고 정책 입안자와 농업 관행을 포괄적인 해결책으로 안내하는 데 추가로 적용될 수 있다. 제4장에서는 컨볼루션 신경망(CNN) 모델을 적용하여 두 개의 구별되는 농업 지역에서 구글 스트리트 뷰(GSV) 이미지를 통해 자동 지상 트러싱의 효과를 탐구한다. 일리노이와 캘리포니아의 센트럴 밸리. 지상 참조 데이터는 감독된 작물 매핑을 위한 필수 전제 조건이다. 저렴하고 효율적인 지상 참조 방법이 없기 때문에 참조 데이터가 광범위하게 제한되고 작물 분류를 방해한다. 본 연구에서는 클라우드 기반 Google 어스 엔진 플랫폼을 사용하여 상태 수준에서 픽셀 기반 크롭 매핑을 수행하여 새로운 지상 참조 기술의 실현 가능성과 신뢰성을 입증한다. 매핑 결과는 미국 농무부(USDA) 작물 데이터층(CDL) 제품을 사용하여 평가된다. 약 130,000개의 GSV 이미지에서 CNN 모델은 약 9,400개의 목표 크롭 이미지를 식별했다. 이 이미지들은 알팔파, 아몬드, 옥수수, 면화, 포도, 쌀, 콩, 피스타치오 등의 작물 유형으로 잘 분류된다. 전체 GSV 이미지 분류 정확도는 센트럴 밸리의 경우 92%, 일리노이 주의 경우 97%이다. 그 후 이미지 지리적 좌표를 특정 방향으로 2~3회 이동하여 31,829개의 크롭 기준점을 생성했다. 즉, 일리노이에서 17,358개, 센트럴 밸리에서 14,471개였다. CDL 제품으로 매핑 결과를 평가한 결과 만족스러운 일관성이 나타났다. GSV에서 파생된 매핑 결과는 2011-2019년 작물 유형 분포의 일반적인 패턴을 포착한다. CDL 제품과 우리의 매핑 결과 사이의 전체 합치는 센트럴 밸리의 경우 0.44–0.99의 R2 값과 일리노이 주의 경우 0.81–0.98의 R2 값으로 표시된다. 제안된 방법의 다른 국가에서 적용 가치를 보여주기 위해, 꽤 좋은 결과를 얻은 한국의 논(2014–2018)을 추가로 매핑했다(R2=0.91). 이러한 결과는 딥 러닝 모델과 함께 사용되는 GSV 이미지가 세계의 많은 지역에서 지상 참조를 위한 효율적이고 비용 효율적인 대체 방법을 제공한다는 것을 나타낸다.1. Abstract 3 LIST OF FIGURES 9 LIST OF TABLES 13 ACKNOWLEDGEMENTS 14 Chapter I. Introduction 15 1.1. Study Background 15 1.2. Purpose of Research 15 Chapter II. Nocturnal stomatal conductance in rice: a coordinating bridge between prior respiration and photosynthesis next dawn 17 Abstract 17 1. Introduction 18 2. Materials and Methods 22 2.1 Plants and growth conditions 22 2.2 Leaf physiological traits 22 2.3 Rapid A/Ci response curves 24 2.4 Stomatal anatomy measurements 24 2.5 Statistical analyses 24 3. Results 25 3.1 Effects of abiotic stress on leaf traits 25 3.2 Nighttime leaf physiological traits 26 3.3 Significant priming effects of gsn on early morning photosynthesis (~5:00 – 7:00) 27 3.4 Path analyses only support the leaf trait coordination 28 3.5 Impacts of gsn on gsd and Amax under light-saturated conditions 29 3.6 Photosynthesis priming effects not detected after mid-morning (9:00) 31 4. Discussion 32 4.1 Abiotic stress results: Implications for different hypotheses 33 4.2 Enhanced carbon assimilation through coordinated regulation by gsn 34 4.3 Evaporative cooling: Passive thermoregulation via leaf trait coordination 36 References 37 Chapter III. Multi-objective optimization of crop yield, water consumption, and greenhouse gases emissions for sustainable rice production 42 Abstract 42 1. Introduction 43 2. Materials and methods 46 2.1 Study site 46 2.2 DNDC model 46 2.3 In situ data 47 2.4 Multi-objective optimization (MOO) algorithm 48 2.5 DNDC-NSGA-III integration and optimization 48 3. Results 50 3.1 DNDC model validation 50 3.2 The gaps between the current farming outcomes and optimized objectives 53 3.3 Approaching Pareto fronts through the heuristic and holistic management 55 3.4 The gaps between current farming practices to potential crop yield with optimal holistic management 56 4. Discussion 58 4.1 Could heuristic and holistic management increase current rice yield with less irrigation water? 58 4.2 Could heuristic and holistic management simultaneously reduce CH4 and N2O emissions? 59 4.3 Limitations and uncertainties 60 Reference 61 Chapter IV. Exploring Google Street View with Deep Learning for Crop Type Mapping 70 Abstract 70 1. Introduction 71 2. Materials and Methods 74 2.1 Study area 74 2.2 General methodology 75 2.3 Google Street View image collection 76 2.4 CNN model training and validation 77 2.5 Producing ground reference data and quality control 79 2.6 Mapping crop types 80 2.7 Mapping results evaluation 81 2.8 Additional test case 82 3. Results 83 3.1 GSV image classification 83 3.2 Producing ground reference data from classified GSV images 84 3.3 Mapping using the GSV derived ground reference 86 4. Discussion 96 4.1 Can we use GSV images to efficiently produce low-cost, sufficient, and reliable crop type ground reference data covering large areas? 96 4.2 Can we use GSV-derived reference data as “ground truth” to map crop types for large areas spanning many years? 97 Appendix 99 References 105 Chapter V. Conclusions 123 Supplementary Information Chapter II 125 Supplementary Information Chapter III 131 Supplementary Information Chapter IV 135 5. Abstract in Korean 138박

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