An analysis of beta cephei stars in NGC 3293 and the effects of stellar rotation

Bibliography: p. 243-248.An intensive photometric study in the Johnson B band of ten β Cephei stars in the open cluster NGC 3293 is presented. High sampling rates of the stars' light curves allow the identification of many formerly unknown pulsation frequencies in these stars, by means of Fourier periodogram analysis. All of the stars are found to be multiperiodic, with up to five frequencies identified for individual stars. Physical parameters of the stars are determined from previous photometric results in the literature. These parameters are used to make a comparison of the observed frequencies with theoretical predictions, including the effects of rotation. The results imply a preference for first and second overtone quadrupole (l=2) pulsation in these stars, while a weak relationship between stellar mass and pulsation overtone is identified. One of the stars is identified as a member of an eclipsing binary system, only the second β Cephei star to be identified as such. Physical parameters of the system are determined. The effects of rapid rotation (specifically, the distortion of the stellar profile from a perfect sphere) on alternative methods of pulsation mode identification are investigated for a model β Cephei star. Ratios between light and velocity amplitudes show extreme differences between rotationally distorted and undistorted models, but these ratios show high sensitivity to other parameters as well. Thermally broadened spectral line profiles are calculated for the same model, for modes with l = 0 to 4 and equatorial rotation velocities up to 400 km/s. Neglect of rotational distortion is shown to lead to underestimation of true equatorial rotation velocities by as much as 253. This result confirms that observed B stars are probably rotating at break-up velocities. In summary, various aspects of the behaviour of the β Cephei stars in NGC 3293 in particular, and of β Cephei stars in general, including rapid rotators, are illuminated and augmented by this study

Real-time 3D Face Recognition using Line Projection and Mesh Sampling

The main contribution of this paper is to present a novel method for automatic 3D face recognition based on sampling a 3D mesh structure in the presence of noise. A structured light method using line projection is employed where a 3D face is reconstructed from a single 2D shot. The process from image acquisition to recognition is described with focus on its real-time operation. Recognition results are presented and it is demonstrated that it can perform recognition in just over one second per subject in continuous operation mode and thus, suitable for real time operation

Duplicated membrane estrogen receptors in the European sea bass (Dicentrarchus labrax): Phylogeny, expression and regulation throughout the reproductive cycle

The numerous estrogen functions reported across vertebrates have been classically explained by their binding to specific transcription factors, the nuclear estrogen receptors (ERs). Rapid non-genomic estrogenic responses have also been recently identified in vertebrates including fish, which can be mediated by membrane receptors such as the G protein-coupled estrogen receptor (Gper). In this study, two genes for Gper, namely gpera and gperb, were identified in the genome of a teleost fish, the European sea bass. Phylogenetic analysis indicated they were most likely retained after the 3R teleost-specific whole genome duplication and raises questions about their function in male and female sea bass. Gpera expression was mainly restricted to brain and pituitary in both sexes while gperb had a widespread tissue distribution with higher expression levels in gill filaments, kidney and head kidney. Both receptors were detected in the hypothalamus and pituitary of both sexes and significant changes in gpers expression were observed throughout the annual reproductive season. In female pituitaries, gpera showed an overall increase in expression throughout the reproductive season while gperb levels remained constant. In the hypothalamus, gpera had a higher expression during vitellogenesis and decreased in fish entering the ovary maturation and ovulation stage, while gperb expression increased at the final atresia stage. In males, gpers expression was constant in the hypothalamus and pituitary throughout the reproductive cycle apart from the mid- to late testicular development stage transition when a significant up-regulation of gpera occurred in the pituitary. The differential sex, seasonal and subtype-specific expression patterns detected for the two novel gper genes in sea bass suggests they may have acquired different and/or complementary roles in mediating estrogens actions in fish, namely on the neuroendocrine control of reproduction.info:eu-repo/semantics/publishedVersio

Towards a methodical framework for comprehensively assessing forest multifunctionality

Funded by Deutsche Forschungsgemeinschaft. Grant Number: DFG FOR 891/1-3 National Natural Science Foundation of China. Grant Numbers: 30710103907, 30930005, 31170457, 31210103910 Swiss National Science Foundation (SNSF) Sino-German Centre for Research Promotion in Beijing. Grant Number: GZ 986Peer reviewedPublisher PD

Categorization of species as native or nonnative using DNA sequence signatures without a complete reference library.

New genetic diagnostic approaches have greatly aided efforts to document global biodiversity and improve biosecurity. This is especially true for organismal groups in which species diversity has been underestimated historically due to difficulties associated with sampling, the lack of clear morphological characteristics, and/or limited availability of taxonomic expertise. Among these methods, DNA sequence barcoding (also known as "DNA barcoding") and by extension, meta-barcoding for biological communities, has emerged as one of the most frequently utilized methods for DNA-based species identifications. Unfortunately, the use of DNA barcoding is limited by the availability of complete reference libraries (i.e., a collection of DNA sequences from morphologically identified species), and by the fact that the vast majority of species do not have sequences present in reference databases. Such conditions are critical especially in tropical locations that are simultaneously biodiversity rich and suffer from a lack of exploration and DNA characterization by trained taxonomic specialists. To facilitate efforts to document biodiversity in regions lacking complete reference libraries, we developed a novel statistical approach that categorizes unidentified species as being either likely native or likely nonnative based solely on measures of nucleotide diversity. We demonstrate the utility of this approach by categorizing a large sample of specimens of terrestrial insects and spiders (collected as part of the Moorea BioCode project) using a generalized linear mixed model (GLMM). Using a training data set of known endemic (n = 45) and known introduced species (n = 102), we then estimated the likely native/nonnative status for 4,663 specimens representing an estimated 1,288 species (412 identified species), including both those specimens that were either unidentified or whose endemic/introduced status was uncertain. Using this approach, we were able to increase the number of categorized specimens by a factor of 4.4 (from 794 to 3,497), and the number of categorized species by a factor of 4.8 from (147 to 707) at a rate much greater than chance (77.6% accuracy). The study identifies phylogenetic signatures of both native and nonnative species and suggests several practical applications for this approach including monitoring biodiversity and facilitating biosecurity

Rapid identification of respiratory bacterial pathogens from bronchoalveolar lavage fluid in cattle by MALDI-TOF MS

Respiratory tract infections are a major health problem and indication for antimicrobial use in cattle and in humans. Currently, most antimicrobial treatments are initiated without microbiological results, holding the risk of inappropriate first intention treatment. The main reason for this empirical treatment is the long turnaround time between sampling and availability of identification and susceptibility results. Therefore the objective of the present study was to develop a rapid identification procedure for pathogenic respiratory bacteria in bronchoalveolar lavage fluid (BALf) samples from cattle by MALDI-TOF MS, omitting the cultivation step on agar plates to reduce the turnaround time between sampling and identification of pathogens. The effects of two different liquid growth media and various concentrations of bacitracin were determined to allow optimal growth of Pasteurellaceae and minimise contamination. The best procedure was validated on 100 clinical BALf samples from cattle with conventional bacterial culture as reference test. A correct identification was obtained in 73% of the samples, with 59.1% sensitivity (Se) (47.2-71.0%) and 100% specificity (Sp) (100-100%) after only 6 hours of incubation. For pure and dominant culture samples, the procedure was able to correctly identify 79.2% of the pathogens, with a sensitivity (Se) of 60.5% (45.0-76.1%) and specificity (Sp) of 100% (100-100%). In mixed culture samples, containing >= 2 clinically relevant pathogens, one pathogen could be correctly identified in 57% of the samples with 57.1%Se (38.8-75.5%) and 100% Sp (100-100%). In conclusion, MALDI-TOF MS is a promising tool for rapid pathogen identification in BALf. This new technique drastically reduces turnaround time and may be a valuable decision support tool to rationalize antimicrobial use

Uranium exploration methodology in cold climates

The uranium prospecting boom of the past decade had, as a major consequence, the rapid development and proliferation of exploration methods for source materials. Numerous established methods were developed and refined whilst new techniques were introduced proving, in some instances, to be highly successful. To the explorationist the proliferation of instrumental hardware and detection systems was something of a headache with the result that in uranium exploration, more so than in other types of prospecting, the choice of exploration method at the appropriate stage of prospecting was frequently ill founded. The situation also spawned ‘black box’ purveyors who made extravagant claims for their equipment. Money was wasted through over kill applications of exploration method accompanied in many instances by deficiencies in the interpretation of results. This project was originally conceived as a means of evaluating, reviewing and filtering from a burgeoning array of systems the most appropriate exploration techniques applicable to cold climate environments. This goal has been trimmed somewhat since it had been hoped to incorporate site investigation data assembled in the field by the writer as appropriate case history material. This was not possible and as a consequence this report is a 'state of the art review' of the applicability of currently available techniques in Arctic and Subarctic environments. Reference is made to published case history data, where appropriate, supportive of the techniques or methods reviewed.Abstract -- Introduction -- Prospecting methods in relation to Arctic and Subarctic environments -- Review of direct exploration methods -- Radiometric methods -- Airborne spectrometry -- Car borne and hand held instrumentation -- Geochemical methods -- Soil and stream sediment methods -- Geobotanical methods -- Water sampling - Hydrogeochemical methods -- Other metods -- Optimal exploration method selection -- References -- Table of exploration methods discussed in this report