DNA methylation profiling of primary neuroblastoma tumors using methyl-CpG-binding domain sequencing

Comprehensive genome-wide DNA methylation studies in neuroblastoma (NB), a childhood tumor that originates from precursor cells of the sympathetic nervous system, are scarce. Recently, we profiled the DNA methylome of 102 well-annotated primary NB tumors by methyl-CpG-binding domain (MBD) sequencing, in order to identify prognostic biomarker candidates. In this data descriptor, we give details on how this data set was generated and which bioinformatics analyses were applied during data processing. Through a series of technical validations, we illustrate that the data are of high quality and that the sequenced fragments represent methylated genomic regions. Furthermore, genes previously described to be methylated in NB are confirmed. As such, these MBD sequencing data are a valuable resource to further study the association of NB risk factors with the NB methylome, and offer the opportunity to integrate methylome data with other -omic data sets on the same tumor samples such as gene copy number and gene expression, also publically available

Advanced Wireless Sensors Used to Monitor the Impact of Environment Design on Human Physiology

This article describes modern wireless sensor devices and their application in the measurements of the human physiology. We used our own advanced ECG Holter device and EEG helmet to record the heart and brain activity impacted by different environments, materials, colors or body positions during work. In this paper, we want to show the interactions between humans and architecture design, which modify human work performance and well-being. This paper is a conclusion of the 3 different pilot studies, where different scopes of human-space interaction were explored. In the experiments, we aimed mostly at wood materials and their beneficial effects on the nervous system. The research in its actual state is primarily focused on optimizing the methods of the ECG data analysis from our Holter device and the EEG data from helmet. Based on these data, we will improve the methodology of the experiments for the next enhanced research with aspiration to automate data analysis

Optimised surface-electrode ion-trap junctions for experiments with cold molecular ions

We discuss the design and optimisation of two types of junctions between surface-electrode radiofrequency ion-trap arrays that enable the integration of experiments with sympathetically cooled molecular ions on a monolithic chip device. A detailed description of a multi-objective optimisation procedure applicable to an arbitrary planar junction is presented, and the results for a cross junction between four quadrupoles as well as a quadrupole-to-octupole junction are discussed. Based on these optimised functional elements, we propose a multi-functional ion-trap chip for experiments with translationally cold molecular ions at temperatures in the millikelvin range. This study opens the door to extending complex chip-based trapping techniques to Coulomb-crystallised molecular ions with potential applications in mass spectrometry, spectroscopy, controlled chemistry and quantum technology.Comment: 19 pages, 10 figure

Between the Lines: documenting the multiple dimensions of computer supported collaborations

When we consider the possibilities for the design and evaluation of Computer Supported Collaborative Learning (CSCL) we probably constrain the CS in CSCL to situations in which learners, or groups of learners collaborate with each other around a single computer, across a local intranet or via the global internet. We probably also consider situations in which the computer itself acts as a collaborative partner giving hints and tips either with or without the addition of an animated pedagogical agent. However, there are now many possibilities for CSCL applications to be offered to learners through computing technology that is something other than a desktop computer, such as the TV or a digital toy. In order to understand how such complex and novel interactions work, we need tools to map out the multiple dimensions of collaboration using a whole variety of technologies. This paper discusses the evolution of a documentation technique for collaborative interactions from its roots in a situation where a single learner is collaborating with a software learning partner, through its second generation: group use of multimedia, to its current test-bed: young children using digital toys and associated software. We will explore some of the challenges these different learning situations pose for those involved in the evaluation of collaborative learning

Single cell analysis reveals immune cell-adipocyte crosstalk regulating the transcription of thermogenic adipocytes.

Immune cells are vital constituents of the adipose microenvironment that influence both local and systemic lipid metabolism. Mice lacking IL10 have enhanced thermogenesis, but the roles of specific cell types in the metabolic response to IL10 remain to be defined. We demonstrate here that selective loss of IL10 receptor α in adipocytes recapitulates the beneficial effects of global IL10 deletion, and that local crosstalk between IL10-producing immune cells and adipocytes is a determinant of thermogenesis and systemic energy balance. Single Nuclei Adipocyte RNA-sequencing (SNAP-seq) of subcutaneous adipose tissue defined a metabolically-active mature adipocyte subtype characterized by robust expression of genes involved in thermogenesis whose transcriptome was selectively responsive to IL10Rα deletion. Furthermore, single-cell transcriptomic analysis of adipose stromal populations identified lymphocytes as a key source of IL10 production in response to thermogenic stimuli. These findings implicate adaptive immune cell-adipocyte communication in the maintenance of adipose subtype identity and function

Restoring a Public Interest Vision of Law in the Age of the Internet

In November 2003, Mr. Marc Rotenberg, Executive Director of the Electronic Privacy Information Center, lectured at Duke Law School on the importance of protecting individual privacy. In his remarks, Mr. Rotenberg recounted the successful campaign against the government\u27s Clipper Chip proposal. He argued that successful public interest advocacy in the Internet age requires the participation of experts from many fields, public engagement, and a willingness to avoid a simple balancing analysis. He further concluded that privacy may be one of the defining issues of a free society in the twenty-first century