Detection of dirt impairments from archived film sequences : survey and evaluations

Film dirt is the most commonly encountered artifact in archive restoration applications. Since dirt usually appears as a temporally impulsive event, motion-compensated interframe processing is widely applied for its detection. However, motion-compensated prediction requires a high degree of complexity and can be unreliable when motion estimation fails. Consequently, many techniques using spatial or spatiotemporal filtering without motion were also been proposed as alternatives. A comprehensive survey and evaluation of existing methods is presented, in which both qualitative and quantitative performances are compared in terms of accuracy, robustness, and complexity. After analyzing these algorithms and identifying their limitations, we conclude with guidance in choosing from these algorithms and promising directions for future research

Proceedings of the NSSDC Conference on Mass Storage Systems and Technologies for Space and Earth Science Applications

The proceedings of the National Space Science Data Center Conference on Mass Storage Systems and Technologies for Space and Earth Science Applications held July 23 through 25, 1991 at the NASA/Goddard Space Flight Center are presented. The program includes a keynote address, invited technical papers, and selected technical presentations to provide a broad forum for the discussion of a number of important issues in the field of mass storage systems. Topics include magnetic disk and tape technologies, optical disk and tape, software storage and file management systems, and experiences with the use of a large, distributed storage system. The technical presentations describe integrated mass storage systems that are expected to be available commercially. Also included is a series of presentations from Federal Government organizations and research institutions covering their mass storage requirements for the 1990's

Focusing on out-of-focus : assessing defocus estimation algorithms for the benefit of automated image masking

Acquiring photographs as input for an image-based modelling pipeline is less trivial than often assumed. Photographs should be correctly exposed, cover the subject sufficiently from all possible angles, have the required spatial resolution, be devoid of any motion blur, exhibit accurate focus and feature an adequate depth of field. The last four characteristics all determine the " sharpness " of an image and the photogrammetric, computer vision and hybrid photogrammetric computer vision communities all assume that the object to be modelled is depicted " acceptably " sharp throughout the whole image collection. Although none of these three fields has ever properly quantified " acceptably sharp " , it is more or less standard practice to mask those image portions that appear to be unsharp due to the limited depth of field around the plane of focus (whether this means blurry object parts or completely out-of-focus backgrounds). This paper will assess how well-or ill-suited defocus estimating algorithms are for automatically masking a series of photographs, since this could speed up modelling pipelines with many hundreds or thousands of photographs. To that end, the paper uses five different real-world datasets and compares the output of three state-of-the-art edge-based defocus estimators. Afterwards, critical comments and plans for the future finalise this paper

The Wiltshire Wills Feasibility Study

The Wiltshire and Swindon Record Office has nearly ninety thousand wills in its care. These records are neither adequately catalogued nor secured against loss by facsimile microfilm copies. With support from the Heritage Lottery Fund the Record Office has begun to produce suitable finding aids for the material. Beginning with this feasibility study the Record Office is developing a strategy to ensure the that facsimiles to protect the collection against risk of loss or damage and to improve public access are created.<p></p> This feasibility study explores the different methodologies that can be used to assist the preservation and conservation of the collection and improve public access to it. The study aims to produce a strategy that will enable the Record Office to create digital facsimiles of the Wills in its care for access purposes and to also create preservation quality microfilms. The strategy aims to seek the most cost effective and time efficient approach to the problem and identifies ways to optimise the processes by drawing on the experience of other similar projects. This report provides a set of guidelines and recommendations to ensure the best use of the resources available for to provide the most robust preservation strategy and to ensure that future access to the Wills as an information resource can be flexible, both local and remote, and sustainable

High-Speed Photoacoustic Microscopy In Vivo

The overarching goal of this research is to develop a novel photoacoustic microscopy: PAM) technology capable of high-speed, high-resolution 3D imaging in vivo. PAM combines the advantages of optical absorption contrast and ultrasonic resolution for deep imaging beyond the quasi-ballistic regime. Its high sensitivity to optical absorption enables the imaging of important physiological parameters, such as hemoglobin concentration and oxygen saturation, which closely correlate with angiogenesis and hypermetabolism--two hallmarks of cancer. To translate PAM to the clinic, both high imaging speed and high spatial resolution are desired. With high spatial resolution, PAM can detect small structural and functional changes early; whereas, high-speed image acquisition helps reduce motion artifacts, patient discomfort, cost, and potentially the risks associated with minimally invasive procedures such as endoscopy and intravascular imaging. To achieve high imaging speed, we have constructed a PAM system using a linear ultrasound array and a kHz-repetition-rate tunable laser. The system has achieved a 249-Hz B-scan rate and a 0.5-Hz 3D imaging rate: over ~6 mm × 10 mm × 3 mm), over 200 times faster than existing mechanical scanning PAM using a single ultrasonic transducer. In addition, high-speed optical-resolution photoacoustic microscopy: OR-PAM) technology has been developed, in which the spatial resolution in one or two dimension(s) is defined by the diffraction-limited optical focus. Using section illumination, the elevational resolution of the system has been improved from ~300 micron to ~28 micron, resulting in a significant improvement in the 3D image quality. Furthermore, multiple optical foci with a microlens array have been used to provide finer than 10-micron lateral resolution--enabling the system to image capillary-level microvessels in vivo--while offering a speed potentially 20 times faster than previously existing single-focus OR-PAM. Finally, potential biomedical applications of the developed technology have been demonstrated through in vivo imaging of murine sentinel lymph nodes, microcirculation dynamics, and human pulsatile dynamics. In the future, this high-speed PAM technology may be adapted for clinical imaging of diabetes-induced vascular complications or tumor angiogenesis, or miniaturized for gastrointestinal or intravascular applications

Human cytomegalovirus uracil DNA glycosylase associates with ppUL44 and accelerates the accumulation of viral DNA

BACKGROUND: Human cytomegalovirus UL114 encodes a uracil-DNA glycosylase homolog that is highly conserved in all characterized herpesviruses that infect mammals. Previous studies demonstrated that the deletion of this nonessential gene delays significantly the onset of viral DNA synthesis and results in a prolonged replication cycle. The gene product, pUL114, also appears to be important in late phase DNA synthesis presumably by introducing single stranded breaks. RESULTS: A series of experiments was performed to formally assign the observed phenotype to pUL114 and to characterize the function of the protein in viral replication. A cell line expressing pUL114 complemented the observed phenotype of a UL114 deletion virus in trans, confirming that the observed defects were the result of a deficiency in this gene product. Stocks of recombinant viruses without elevated levels of uracil were produced in the complementing cells; however they retained the phenotype of poor growth in normal fibroblasts suggesting that poor replication was unrelated to uracil content of input genomes. Recombinant viruses expressing epitope tagged versions of this gene demonstrated that pUL114 was expressed at early times and that it localized to viral replication compartments. This protein also coprecipitated with the DNA polymerase processivity factor, ppUL44 suggesting that these proteins associate in infected cells. This apparent interaction did not appear to require other viral proteins since ppUL44 could recruit pUL114 to the nucleus in uninfected cells. An analysis of DNA replication kinetics revealed that the initial rate of DNA synthesis and the accumulation of progeny viral genomes were significantly reduced compared to the parent virus. CONCLUSION: These data suggest that pUL114 associates with ppUL44 and that it functions as part of the viral DNA replication complex to increase the efficiency of both early and late phase viral DNA synthesis

Amino terminal tyrosine phosphorylation of human MIXL1

Seven members of the Mix family of paired-type homeoproteins regulate mesoderm/endoderm differentiation in amphibians. In mammals, the MIXL1 (Mix. 1 homeobox [Xenopus laevis]-like gene 1) gene is the sole representative of this family. Unlike the amphibian Mix genes that encode an open reading frame of >300 amino acids, mammalian MIXL1 encodes a smaller protein (~230aa). However, mammalian MIXL1 contains a unique proline-rich domain (PRD) with a potential to interact with signal transducing Src homolgy 3 (SH3) domains. Notably, human MIXL1 also contains a unique tyrosine residue Tyr20 that is amino-terminal to the PRD. Here we report that mammalian MIXL1 protein is phosphorylated at Tyr20 and the phosphorylation is dramatically reduced in the absence of PRD. Our findings are consistent with Tyr20 phosphorylation of MIXL1 being a potential regulatory mechanism that governs its activity

Second Conference on NDE for Aerospace Requirements

Nondestructive evaluation and inspection procedures must constantly improve rapidly in order to keep pace with corresponding advances being made in aerospace material and systems. In response to this need, the 1989 Conference was organized to provide a forum for discussion between the materials scientists, systems designers, and NDE engineers who produce current and future aerospace systems. It is anticipated that problems in current systems can be resolved more quickly and that new materials and structures can be designed and manufactured in such a way as to be more easily inspected and to perform reliably over the life cycle of the system