61 research outputs found
A Deterministic Model for One-Dimensional Excluded Flow with Local Interactions
Natural phenomena frequently involve a very large number of interacting
molecules moving in confined regions of space. Cellular transport by motor
proteins is an example of such collective behavior. We derive a deterministic
compartmental model for the unidirectional flow of particles along a
one-dimensional lattice of sites with nearest-neighbor interactions between the
particles. The flow between consecutive sites is governed by a soft simple
exclusion principle and by attracting or repelling forces between neighboring
particles. Using tools from contraction theory, we prove that the model admits
a unique steady-state and that every trajectory converges to this steady-state.
Analysis and simulations of the effect of the attracting and repelling forces
on this steady-state highlight the crucial role that these forces may play in
increasing the steady-state flow, and reveal that this increase stems from the
alleviation of traffic jams along the lattice. Our theoretical analysis
clarifies microscopic aspects of complex multi-particle dynamic processes
Early Replicons: Origin and Evolution
RNA and protein molecules were found to be both templates for replication and specific catalysts for biochemical reactions. RNA molecules, although very difficult to obtain via plausible synthetic pathways under prebiotic conditions, are the only candidates for early replicons. Only they are obligatory templates for replication, which can conserve mutations and propagate them to forthcoming generations. RNA based catalysts, called ribozymes, act with high efficiency and specificity for all classes of reactions involved in the interconversion of RNA molecules such as cleavage and template assisted ligation. The idea of an RNA world was conceived for a plausible prebiotic scenario of RNA molecules operating upon each other and constituting thereby a functional molecular organization. A theoretical account on molecular replication making precise the conditions under which one observes parabolic, exponential or hyperbolic growth is presented. Exponential growth is observed in a protein assisted RNA world where plus-minus-(±)-duplex formation is avoided by the action of an RNA replicase. Error propagation to forthcoming generations is analyzed in absence of selective neutral mutants as well as for predefined degrees of neutrality. The concept of an error threshold for sufficiently precise replication and survival of populations derived from the theory of molecular quasispecies is discussed. Computer simulations are used to model the interplay between adaptive evolution and random drift. A model of evolution is proposed that allows for explicit handling of phenotypes
Intracellular transport driven by cytoskeletal motors: General mechanisms and defects
Cells are strongly out-of-equilibrium systems driven by continuous energy
supply. They carry out many vital functions requiring active transport of
various ingredients and organelles, some being small, others being large. The
cytoskeleton, composed of three types of filaments, determines the shape of the
cell and plays a role in cell motion. It also serves as a road network for the
so-called cytoskeletal motors. These molecules can attach to a cytoskeletal
filament, perform directed motion, possibly carrying along some cargo, and then
detach. It is a central issue to understand how intracellular transport driven
by molecular motors is regulated, in particular because its breakdown is one of
the signatures of some neuronal diseases like the Alzheimer.
We give a survey of the current knowledge on microtubule based intracellular
transport. We first review some biological facts obtained from experiments, and
present some modeling attempts based on cellular automata. We start with
background knowledge on the original and variants of the TASEP (Totally
Asymmetric Simple Exclusion Process), before turning to more application
oriented models. After addressing microtubule based transport in general, with
a focus on in vitro experiments, and on cooperative effects in the
transportation of large cargos by multiple motors, we concentrate on axonal
transport, because of its relevance for neuronal diseases. It is a challenge to
understand how this transport is organized, given that it takes place in a
confined environment and that several types of motors moving in opposite
directions are involved. We review several features that could contribute to
the efficiency of this transport, including the role of motor-motor
interactions and of the dynamics of the underlying microtubule network.
Finally, we discuss some still open questions.Comment: 74 pages, 43 figure
Recognizing and engineering digital-like logic gates and switches in gene regulatory networks
A central aim of synthetic biology is to build organisms that can perform useful activities in response to specified conditions. The digital computing paradigm which has proved so successful in electrical engineering is being mapped to synthetic biological systems to allow them to make such decisions. However, stochastic molecular processes have graded input-output functions, thus, bioengineers must select those with desirable characteristics and refine their transfer functions to build logic gates with digital-like switching behaviour. Recent efforts in genome mining and the development of programmable RNA-based switches, especially CRISPRi, have greatly increased the number of parts available to synthetic biologists. Improvements to the digital characteristics of these parts are required to enable robust predictable design of deeply layered logic circuits
Isolierung und Charakterisierung von Myxobakterien und anderen seltenen Meeresbakterien und deren Multilocus-Sequenzanalyse der indonesischen BiodiversitÀt
Indonesia is a country with high potential in biodiversity exploration, especially for microorganisms. This study used samples collected from the islands of Java, Sulawesi, and Bali under the Germany-Indonesia Antiinfectives Cooperation (GINAICO) project. We have isolated 59 strains of gliding bacteria, 98% of which are myxobacteria while the remaining 2% is made up of other gliding bacteria. In this research, we have identified a rare myxobacterium and a novel non-myxobacterial strain from our selected samples. Finally, we confirmed the effectiveness of housekeeping genes as a core method in analysing the degree of relatedness between different strains. The strain Soce1964KM was found to be closest to the rare genus Sorangium and strain 1932KM was identified as a novel genus in the family Flammeovirgaceae. Both results are according to the analysis of its 16S rRNA gene sequence and whole-genome sequencing analysis for strain 1932KM. We also performed screening for antiinfectives against Escherichia coli, Candida albicans and Staphylococcus aureus. Strain Soce1964KM has the highest activity against Staphylococcus aureus and Candida albicans. Analysis of the methanol extract of Soce1964KM through the chromatogram from High-Pressure Liquid Chromatography (HPLC) and High-Resolution Electrospray Ionisation Mass Spectrometry (HRESIMS)âin addition to the database in Myxobaseâshowed that the extract contains a known compound, namely Disorazol. We found 1932KM to be a novel strain after an in-depth examination of this strainâs phenotype and genotype as part of a polyphasic taxonomy analysis. Also, we conducted a gene cluster analysis and bioassay against pathogenic bacteria and statistical analysis of 16S rRNA gene sequences from this strain with the statistical tool PAST. Bioinformatic tools, such as Prokka, RAST, AntiSmash, NP.searcher, PubChem, SMART and Molinspiration, were used for an in-depth analysis of the whole-genome sequence. Strain 1932KM has also obtained a deposition number from the Indonesian Culture Collections, namely InaCC B1242 with the proposed name of Balibacter flavus gen.nov.sp.nov. Meanwhile, we used the housekeeping genes pgm, pyrG and rpoB to analyse the relatedness between the genera Corallococcus and Myxococcus. These housekeeping genes were used in this study as the core to analyse the similarities between the isolated strains and the type strains. The analysis involved the reconstruction of a phylogenetic tree and its polymorphisms. Bioinformatics tools supported these analyses. These analyses showed that the method is suitable for preliminary analysis in the search for novel species and/or subspecies.Indonesien ist ein Land mit hohem Potenzial fĂŒr die Erforschung der biologischen Vielfalt, insbesondere fĂŒr Mikroorganismen. In dieser Studie wurden Proben verwendet, die auf den Inseln Java, Sulawesi, und Bali im Rahmen des Projekts GINAICO (Deutschland-Indonesien Antiinfectives Cooperation) gesammelt wurden. Wir haben 59 StĂ€mme gleitender Bakterien isoliert, von denen 98% Myxobakterien sind, wĂ€hrend die restlichen 2% aus anderen gleitenden Bakterien bestehen. In dieser Studie haben wir aus unseren ausgewĂ€hlten Proben ein seltenes Myxobakterium und einen neuartigen nicht-myxobakteriellen Stamm identifiziert. SchlieĂlich bestĂ€tigten wir die Wirksamkeit von Housekeeping-Genen als Kernmethode bei der Analyse des Grads der Verwandtschaft zwischen verschiedenen StĂ€mmen. Es wurde festgestellt, dass der Stamm Soce1964KM der seltenen Gattung Sorangium am nĂ€chsten kommt, und der Stamm 1932KM wurde als neuartige Gattung in der Familie der Flammeovirgaceae identifiziert. Beide Ergebnisse entsprechen der Analyse seiner 16S-rRNA-Gensequenz und der Gesamtgenomsequenzierungsanalyse fĂŒr den Stamm 1932KM. Wir fĂŒhrten auch ein Screening auf Antiinfektiva gegen Escherichia coli, Candida albicans und Staphylococcus aureus durch. Der Stamm Soce1964KM hat die höchste AktivitĂ€t gegen Staphylococcus aureus und Candida albicans. Die Analyse des Methanolextrakts von Soce1964KM mittels Chromatogramms aus HochdruckflĂŒssigchromatographie (HPLC) und hochauflösender Elektrospray-Ionisationsmassenspektrometrie (HRESIMS) - zusĂ€tzlich zur Datenbank in Myxobase - zeigte, dass der Extrakt eine bekannte Verbindung enthĂ€lt, nĂ€mlich Disorazol. Nach einer eingehenden Untersuchung des PhĂ€notyps und Genotyps dieses Stammes im Rahmen einer mehrphasigen Taxonomieanalyse stellten wir fest, dass 1932KM ein neuartiger Stamm ist. AuĂerdem fĂŒhrten wir mit dem statistischen Tool PAST eine Genclusteranalyse und einen Bioassay gegen pathogene Bakterien sowie eine statistische Analyse der 16S-rRNA-Gensequenzen dieses Stammes durch. Bioinformatische Werkzeuge wie Prokka, RAST, AntiSmash, NP.searcher, PubChem, SMART und Molinspiration wurden fĂŒr eine eingehende Analyse der gesamten Genomsequenz verwendet. Der Stamm 1932KM hat auch eine Hinterlegungsnummer aus den indonesischen Kultursammlungen erhalten, nĂ€mlich InaCC B1242 mit dem vorgeschlagenen Namen Balibacter flavus gen.nov.sp.nov. In der Zwischenzeit haben wir die Housekeeping-Gene pgm, pyrG und rpoB verwendet, um die Verwandtschaft zwischen den Gattungen Corallococcus und Myxococcus zu analysieren. Diese Housekeeping-Gene wurden in dieser Studie als Kern verwendet, um die Ăhnlichkeiten zwischen den isolierten StĂ€mmen und den TypstĂ€mmen zu analysieren. Die Analyse umfasste die Rekonstruktion eines phylogenetischen Baums und seiner Polymorphismen. Bioinformatik-Tools unterstĂŒtzten diese Analysen. Diese Untersuchungen zeigten, dass die Methode fĂŒr eine vorlĂ€ufige Analyse bei der Suche nach neuen Arten und / oder Unterarten geeignet ist
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