30,439 research outputs found
Towards a genome-wide transcriptogram: the Saccharomyces cerevisiae case
A genome modular classification that associates cellular processes to modules could lead to a method to quantify the differences in gene expression levels in different cellular stages or conditions: the transcriptogram, a powerful tool for assessing cell performance, would be at hand. Here we present a computational method to order genes on a line that clusters strongly interacting genes, defining functional modules associated with gene ontology terms. The starting point is a list of genes and a matrix specifying their interactions, available at large gene interaction databases. Considering the Saccharomyces cerevisiae genome we produced a succession of plots of gene transcription levels for a fermentation process. These plots discriminate the fermentation stage the cell is going through and may be regarded as the first versions of a transcriptogram. This method is useful for extracting information from cell stimuli/responses experiments, and may be applied with diagnostic purposes to different organisms
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Evolution of substrate-specific gene expression and RNA editing in brown rot wood-decaying fungi.
Fungi that decay wood have characteristic associations with certain tree species, but the mechanistic bases for these associations are poorly understood. We studied substrate-specific gene expression and RNA editing in six species of wood-decaying fungi from the 'Antrodia clade' (Polyporales, Agaricomycetes) on three different wood substrates (pine, spruce, and aspen) in submerged cultures. We identified dozens to hundreds of substrate-biased genes (i.e., genes that are significantly upregulated in one substrate relative to the other two substrates) in each species, and these biased genes are correlated with their host ranges. Evolution of substrate-biased genes is associated with gene family expansion, gain and loss of genes, and variation in cis- and trans- regulatory elements, rather than changes in protein coding sequences. We also demonstrated widespread RNA editing events in the Antrodia clade, which differ from those observed in the Ascomycota in their distribution, substitution types, and the genomic environment. Moreover, we found that substrates could affect editing positions and frequency, including editing events occurring in mRNA transcribed from wood-decay-related genes. This work shows the extent to which gene expression and RNA editing differ among species and substrates, and provides clues into mechanisms by which wood-decaying fungi may adapt to different hosts
Phenotypic landscape inference reveals multiple evolutionary paths to C photosynthesis
C photosynthesis has independently evolved from the ancestral C
pathway in at least 60 plant lineages, but, as with other complex traits, how
it evolved is unclear. Here we show that the polyphyletic appearance of C
photosynthesis is associated with diverse and flexible evolutionary paths that
group into four major trajectories. We conducted a meta-analysis of 18 lineages
containing species that use C, C, or intermediate C-C forms of
photosynthesis to parameterise a 16-dimensional phenotypic landscape. We then
developed and experimentally verified a novel Bayesian approach based on a
hidden Markov model that predicts how the C phenotype evolved. The
alternative evolutionary histories underlying the appearance of C
photosynthesis were determined by ancestral lineage and initial phenotypic
alterations unrelated to photosynthesis. We conclude that the order of C
trait acquisition is flexible and driven by non-photosynthetic drivers. This
flexibility will have facilitated the convergent evolution of this complex
trait
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