Radiation Tolerance of Fully-Depleted P-Channel CCDs Designed for the SNAP Satellite

Thick, fully depleted p-channel charge-coupled devices (CCDs) have been developed at the Lawrence Berkeley National Laboratory (LBNL). These CCDs have several advantages over conventional thin, n-channel CCDs, including enhanced quantum efficiency and reduced fringing at near-infrared wavelengths and improved radiation tolerance. Here we report results from the irradiation of CCDs with 12.5 and 55 MeV protons at the LBNL 88-Inch Cyclotron and with 0.1-1 MeV electrons at the LBNL Co60 source. These studies indicate that the LBNL CCDs perform well after irradiation, even in the parameters in which significant degradation is observed in other CCDs: charge transfer efficiency, dark current, and isolated hot pixels. Modeling the radiation exposure over a six-year mission lifetime with no annealing, we expect an increase in dark current of 20 e/pixel/hr, and a degradation of charge transfer efficiency in the parallel direction of 3e-6 and 1e-6 in the serial direction. The dark current is observed to improve with an annealing cycle, while the parallel CTE is relatively unaffected and the serial CTE is somewhat degraded. As expected, the radiation tolerance of the p-channel LBNL CCDs is significantly improved over the conventional n-channel CCDs that are currently employed in space-based telescopes such as the Hubble Space Telescope.Comment: 11 pages, 10 figures, submitted to IEEE Transaction

Microchannel Plates for the UVCS and SUMER Instruments on the SOHO Satellite

The microchannel plates for the detectors in the SUMER (Solar Ultraviolet Measurements of Emitted Radiation) and UVCS (Ultraviolet Coronograph Spectrometer) instruments aboard the Solar Orbiting Heliospheric Observatory (SOHO) mission to be launched in late 1995 are described. A low resistance Z stack of microchannel plates (MCP's) is employed in a detector format of 27 mm x 10 mm using a multilayer cross delay line anode (XDL) with 1024 x 360 digitized pixels. The MCP stacks provide gains of greater than 2 x 10(exp 7) with good pulse height distributions (as low as 25% FWHM) under uniform flood illumination. Background rates of approx. 0.6 event cm(exp -2) sec(exp -1) are obtained for this configuration. Local counting rates up to about 800 events/pixel/sec have been achieved with little drop of the MCP gain. MCP preconditioning results are discussed, showing that some MCP stacks fail to have gain decreases when subjected to a high flux UV scrub. Also, although the bare MCP quantum efficiencies are close to those expected (10%), we found that the long wavelength response of KBr photocathodes could be substantially enhanced by the MCP scrubbing process. Flat field images are characterized by a low level of MCP fixed pattern noise and are stable. Preliminary calibration results for the instruments are shown

Soils of the Central Nebraska Loess Hills and Central Loess Plains

Understanding soil systems that characterize a region is critical to natural resource management. However, the knowledge gained through intensive study of local soil systems, which takes place annually as part of collegiate soil judging contests, is often poorly preserved for future use. In this study, field descriptions and laboratory data for 16 soil profiles described for the 2019 Region 5 Soil Judging Contest were used to characterize the soil system of the Central Nebraska Loess Hills and Central Loess Plains. Three landscape components of this soil system were analyzed: the loess uplands and rainwater basins, the transitional zone, and bottomlands. Rainwater basins exhibit increasing clay, clay films, and melanization compared to surrounding uplands. The transitional zone between the upland and bottomlands exhibits fining and aging of parent material with increasing elevation, resulting in increased prevalence of clay films, lower pH relative to parent material, and melanization. The bottomlands exhibited subtle variations in texture of the alluvium resulting in differences in expression of melanization, effervescence, pH, and redoximorphic features. Patterns observed within this soil system are well explained by existing pedogenic theories and demonstrate the unique interplay between soil-forming factors characteristic of this region of the Great Plains

Regulation of gene expression in human tendinopathy

<p>Abstract</p> <p>Background</p> <p>Chronic tendon injuries, also known as tendinopathies, are common among professional and recreational athletes. These injuries result in a significant amount of morbidity and health care expenditure, yet little is known about the molecular mechanisms leading to tendinopathy.</p> <p>Methods</p> <p>We have used histological evaluation and molecular profiling to determine gene expression changes in 23 human patients undergoing surgical procedures for the treatment of chronic tendinopathy.</p> <p>Results</p> <p>Diseased tendons exhibit altered extracellular matrix, fiber disorientation, increased cellular content and vasculature, and the absence of inflammatory cells. Global gene expression profiling identified 983 transcripts with significantly different expression patterns in the diseased tendons. Global pathway analysis further suggested altered expression of extracellular matrix proteins and the lack of an appreciable inflammatory response.</p> <p>Conclusions</p> <p>Identification of the pathways and genes that are differentially regulated in tendinopathy samples will contribute to our understanding of the disease and the development of novel therapeutics.</p

Performance results of the GALEX cross delay line detectors

We describe the performance results for the Galaxy Evolution Explorer (GALEX) far ultraviolet (FUV) and near ultraviolet (NUV) detectors. The detectors were delivered to JPL/Caltech starting in the fall of 2000 and have undergone approximately 1000 hours of pre-flight system-level testing to date. The GALEX detectors are sealed tube micro-channel plate (MCP) delay line readout detectors. They have a 65 mm diameter active area, which will be the largest format on orbit. The FUV detector has a spectral bandpass from 115 - 180 nm and the NUV detector has a bandpass from 165 - 300 nm. We report here on the performance of the detectors before and after integration into the instrument. Characteristics measured include the background count rate and distribution, gain vs. applied high voltage, spatial resolution and linearity, flat fields, and quantum efficiency

Towards a genome-wide transcriptogram: the Saccharomyces cerevisiae case

A genome modular classification that associates cellular processes to modules could lead to a method to quantify the differences in gene expression levels in different cellular stages or conditions: the transcriptogram, a powerful tool for assessing cell performance, would be at hand. Here we present a computational method to order genes on a line that clusters strongly interacting genes, defining functional modules associated with gene ontology terms. The starting point is a list of genes and a matrix specifying their interactions, available at large gene interaction databases. Considering the Saccharomyces cerevisiae genome we produced a succession of plots of gene transcription levels for a fermentation process. These plots discriminate the fermentation stage the cell is going through and may be regarded as the first versions of a transcriptogram. This method is useful for extracting information from cell stimuli/responses experiments, and may be applied with diagnostic purposes to different organisms

Defining the inflammatory signature of human lung explant tissue in the presence and absence of glucocorticoid

Background: Airway inflammation is a feature of many respiratory diseases and there is a need for newer, more effective anti-inflammatory compounds. The aim of this study was to develop an ex vivo human lung explant model which can be used to help study the mechanisms underlying inflammatory responses and which can provide a tool to aid drug discovery for inflammatory respiratory diseases such as asthma and COPD. Method: Parenchymal lung tissue from 6 individual donors was dissected and cultured with two pro-inflammatory stimuli, lipopolysaccharide (LPS) (1 µg/ml) and interleukin-1 beta (IL-1β) (10 ng/ml) in the presence or absence of dexamethasone (1 µM). Inflammatory responses were assessed using Luminex analysis of tissue culture supernatants to measure levels of 21 chemokines, growth factors and cytokines. Results: A robust and reproducible inflammatory signal was detected across all donors for 12 of the analytes measured following LPS stimulation with a modest fold increase (4-fold in CCL3, CCL4, GM-CSF, IL-10, TNF-α and IL-1β. The inflammatory signal induced by IL-1β stimulation was less than that observed with LPS but resulted in elevated levels of 7 analytes (CXCL8, CCL3, CCL4, GM-CSF, IL-6, IL-10 and TNF-α). The inflammatory responses induced by both stimulations was supressed by dexamethasone for the majority of analytes. Conclusions: These data provide proof of concept that this ex vivo human lung explant model is responsive to inflammatory signals and could be used to investigate the anti-inflammatory effects of existing and novel compounds. In addition this model could be used to help define the mechanisms and pathways involved in development of inflammatory airway disease

The Desi Spectrograph System and Production

The Dark Energy Spectroscopic Instrument (DESI) is a project in construction to measure the expansion history of the Universe using the Baryon Acoustic Oscillation technique. The spectra of 35 million galaxies and quasars over 14,000 square degrees will be measured during the life of the experiment. A new prime focus corrector for the KPNO Mayall telescope will deliver light to 5000 fiber optic positioners. The fibers in turn feed ten broad-band spectrographs covering a 360 - 980 nm passband with a spectral resolution (λ/Δλ) between 1500 and 4000. The spectrograph uses two dichroic beam splitters to separate the flux among three spectral cameras, each with a volume phase holographic grating and lens system that focuses onto a charge coupled device detector. We describe the spectrograph, its system requirements, design and construction