Communications Biophysics

Contains research objectives and summary of research on nine research projects split into four sections.National Institutes of Health (Grant 5 ROI NS11000-03)National Institutes of Health (Grant 1 P01 NS13126-01)National Institutes of Health (Grant 1 RO1 NS11153-01)National Institutes of Health (Grant 2 R01 NS10916-02)Harvard-M.I.T. Rehabilitation Engineering CenterU. S. Department of Health, Education, and Welfare (Grant 23-P-55854)National Institutes of Health (Grant 1 ROl NS11680-01)National Institutes of Health (Grant 5 ROI NS11080-03)M.I.T. Health Sciences Fund (Grant 76-07)National Institutes of Health (Grant 5 T32 GM07301-02)National Institutes of Health (Grant 5 TO1 GM01555-10

A multimodal neuroimaging study of somatosensory system

The thesis is the result of a training by the Magnetoencephalography (MEG)-lab by the Center mind/brain science of the university of Trento. Final goal of the analysis was answering the question if MEG is capable to capture activities from the subcortical brain areas and to follow the neural information flow up along the fibers to the cortex. First aim of the thesis is describing the project and developing of an experiment on the somatosensory system that I executed by the CIMeC. The somatosensory system was activated by applying electrical stimulation to the median nerve and MEG signal during this stimulation was recorded. Also MRI and diffusion MRI data of the subject were collected. Further aim of the thesis is to describe the analysis I executed on the collected data. For this purpose the MEG source localization was executed and also Monte-Carlo simulation. The data obtained were integrated with the information obtained from diffusion MRI. Satisfactory results were obtained although we could not prove definitely the result

Advances in point process filters and their application to sympathetic neural activity

This thesis is concerned with the development of techniques for analyzing the sequences of stereotypical electrical impulses within neurons known as spikes. Sequences of spikes, also called spike trains, transmit neural information; decoding them often provides details about the physiological processes generating the neural activity. Here, the statistical theory of event arrivals, called point processes, is applied to human muscle sympathetic spike trains, a peripheral nerve signal responsible for cardiovascular regulation. A novel technique that uses observed spike trains to dynamically derive information about the physiological processes generating them is also introduced. Despite the emerging usage of individual spikes in the analysis of human muscle sympathetic nerve activity, the majority of studies in this field remain focused on bursts of activity at or below cardiac rhythm frequencies. Point process theory applied to multi-neuron spike trains captured both fast and slow spiking rhythms. First, analysis of high-frequency spiking patterns within cardiac cycles was performed and, surprisingly, revealed fibers with no cardiac rhythmicity. Modeling spikes as a function of average firing rates showed that individual nerves contribute substantially to the differences in the sympathetic stressor response across experimental conditions. Subsequent investigation of low-frequency spiking identified two physiologically relevant frequency bands, and modeling spike trains as a function of hemodynamic variables uncovered complex associations between spiking activity and biophysical covariates at these two frequencies. For example, exercise-induced neural activation enhances the relationship of spikes to respiration but does not affect the extremely precise alignment of spikes to diastolic blood pressure. Additionally, a novel method of utilizing point process observations to estimate an internal state process with partially linear dynamics was introduced. Separation of the linear components of the process model and reduction of the sampled space dimensionality improved the computational efficiency of the estimator. The method was tested on an established biophysical model by concurrently computing the dynamic electrical currents of a simulated neuron and estimating its conductance properties. Computational load reduction, improved accuracy, and applicability outside neuroscience establish the new technique as a valuable tool for decoding large dynamical systems with linear substructure and point process observations

Aerospace Medicine and Biology. A continuing bibliography with indexes

This bibliography lists 244 reports, articles, and other documents introduced into the NASA scientific and technical information system in February 1981. Aerospace medicine and aerobiology topics are included. Listings for physiological factors, astronaut performance, control theory, artificial intelligence, and cybernetics are included

Neuropathy Classification of Corneal Nerve Images Using Artificial Intelligence

Nerve variations in the human cornea have been associated with alterations in the neuropathy state of a patient suffering from chronic diseases. For some diseases, such as diabetes, detection of neuropathy prior to visible symptoms is important, whereas for others, such as multiple sclerosis, early prediction of disease worsening is crucial. As current methods fail to provide early diagnosis of neuropathy, in vivo corneal confocal microscopy enables very early insight into the nerve damage by illuminating and magnifying the human cornea. This non-invasive method captures a sequence of images from the corneal sub-basal nerve plexus. Current practices of manual nerve tracing and classification impede the advancement of medical research in this domain. Since corneal nerve analysis for neuropathy is in its initial stages, there is a dire need for process automation. To address this limitation, we seek to automate the two stages of this process: nerve segmentation and neuropathy classification of images. For nerve segmentation, we compare the performance of two existing solutions on multiple datasets to select the appropriate method and proceed to the classification stage. Consequently, we approach neuropathy classification of the images through artificial intelligence using Adaptive Neuro-Fuzzy Inference System, Support Vector Machines, Naïve Bayes and k-nearest neighbors. We further compare the performance of machine learning classifiers with deep learning. We ascertained that nerve segmentation using convolutional neural networks provided a significant improvement in sensitivity and false negative rate by at least 5% over the state-of-the-art software. For classification, ANFIS yielded the best classification accuracy of 93.7% compared to other classifiers. Furthermore, for this problem, machine learning approaches performed better in terms of classification accuracy than deep learning

Methods of Optimizing Speech Enhancement for Hearing Applications

Speech intelligibility in hearing applications suffers from background noise. One of the most effective solutions is to develop speech enhancement algorithms based on the biological traits of the auditory system. In humans, the medial olivocochlear (MOC) reflex, which is an auditory neural feedback loop, increases signal-in-noise detection by suppressing cochlear response to noise. The time constant is one of the key attributes of the MOC reflex as it regulates the variation of suppression over time. Different time constants have been measured in nonhuman mammalian and human auditory systems. Physiological studies reported that the time constant of nonhuman mammalian MOC reflex varies with the properties (e.g. frequency, bandwidth) changes of the stimulation. A human based study suggests that time constant could vary when the bandwidth of the noise is changed. Previous works have developed MOC reflex models and successfully demonstrated the benefits of simulating the MOC reflex for speech-in-noise recognition. However, they often used fixed time constants. The effect of the different time constants on speech perception remains unclear. The main objectives of the present study are (1) to study the effect of the MOC reflex time constant on speech perception in different noise conditions; (2) to develop a speech enhancement algorithm with dynamic time constant optimization to adapt to varying noise conditions for improving speech intelligibility. The first part of this thesis studies the effect of the MOC reflex time constants on speech-in-noise perception. Conventional studies do not consider the relationship between the time constants and speech perception as it is difficult to measure the speech intelligibility changes due to varying time constants in human subjects. We use a model to investigate the relationship by incorporating Meddis’ peripheral auditory model (which includes a MOC reflex) with an automatic speech recognition (ASR) system. The effect of the MOC reflex time constant is studied by adjusting the time constant parameter of the model and testing the speech recognition accuracy of the ASR. Different time constants derived from human data are evaluated in both speech-like and non-speech like noise at the SNR levels from -10 dB to 20 dB and clean speech condition. The results show that the long time constants (≥1000 ms) provide a greater improvement of speech recognition accuracy at SNR levels≤10 dB. Maximum accuracy improvement of 40% (compared to no MOC condition) is shown in pink noise at the SNR of 10 dB. Short time constants (<1000 ms) show recognition accuracy over 5% higher than the longer ones at SNR levels ≥15 dB. The second part of the thesis develops a novel speech enhancement algorithm based on the MOC reflex with a time constant that is dynamically optimized, according to a lookup table for varying SNRs. The main contributions of this part include: (1) So far, the existing SNR estimation methods are challenged in cases of low SNR, nonstationary noise, and computational complexity. High computational complexity would increase processing delay that causes intelligibility degradation. A variance of spectral entropy (VSE) based SNR estimation method is developed as entropy based features have been shown to be more robust in the cases of low SNR and nonstationary noise. The SNR is estimated according to the estimated VSE-SNR relationship functions by measuring VSE of noisy speech. Our proposed method has an accuracy of 5 dB higher than other methods especially in the babble noise with fewer talkers (2 talkers) and low SNR levels (< 0 dB), with averaging processing time only about 30% of the noise power estimation based method. The proposed SNR estimation method is further improved by implementing a nonlinear filter-bank. The compression of the nonlinear filter-bank is shown to increase the stability of the relationship functions. As a result, the accuracy is improved by up to 2 dB in all types of tested noise. (2) A modification of Meddis’ MOC reflex model with a time constant dynamically optimized against varying SNRs is developed. The model incudes simulated inner hair cell response to reduce the model complexity, and now includes the SNR estimation method. Previous MOC reflex models often have fixed time constants that do not adapt to varying noise conditions, whilst our modified MOC reflex model has a time constant dynamically optimized according to the estimated SNRs. The results show a speech recognition accuracy of 8 % higher than the model using a fixed time constant of 2000 ms in different types of noise. (3) A speech enhancement algorithm is developed based on the modified MOC reflex model and implemented in an existing hearing aid system. The performance is evaluated by measuring the objective speech intelligibility metric of processed noisy speech. In different types of noise, the proposed algorithm increases intelligibility at least 20% in comparison to unprocessed noisy speech at SNRs between 0 dB and 20 dB, and over 15 % in comparison to processed noisy speech using the original MOC based algorithm in the hearing aid

Analysis of Retinal Image Data to Support Glaucoma Diagnosis

Fundus kamera je široce dostupné zobrazovací zařízení, které umožňuje relativně rychlé a nenákladné vyšetření zadního segmentu oka – sítnice. Z těchto důvodů se mnoho výzkumných pracovišť zaměřuje právě na vývoj automatických metod diagnostiky nemocí sítnice s využitím fundus fotografií. Tato dizertační práce analyzuje současný stav vědeckého poznání v oblasti diagnostiky glaukomu s využitím fundus kamery a navrhuje novou metodiku hodnocení vrstvy nervových vláken (VNV) na sítnici pomocí texturní analýzy. Spolu s touto metodikou je navržena metoda segmentace cévního řečiště sítnice, jakožto další hodnotný příspěvek k současnému stavu řešené problematiky. Segmentace cévního řečiště rovněž slouží jako nezbytný krok předcházející analýzu VNV. Vedle toho práce publikuje novou volně dostupnou databázi snímků sítnice se zlatými standardy pro účely hodnocení automatických metod segmentace cévního řečiště.Fundus camera is widely available imaging device enabling fast and cheap examination of the human retina. Hence, many researchers focus on development of automatic methods towards assessment of various retinal diseases via fundus images. This dissertation summarizes recent state-of-the-art in the field of glaucoma diagnosis using fundus camera and proposes a novel methodology for assessment of the retinal nerve fiber layer (RNFL) via texture analysis. Along with it, a method for the retinal blood vessel segmentation is introduced as an additional valuable contribution to the recent state-of-the-art in the field of retinal image processing. Segmentation of the blood vessels also serves as a necessary step preceding evaluation of the RNFL via the proposed methodology. In addition, a new publicly available high-resolution retinal image database with gold standard data is introduced as a novel opportunity for other researches to evaluate their segmentation algorithms.

Communications Biophysics

Contains research objectives, summary of research and reports on three research projects.National Institutes of Health (Grant 5 PO1 GM14940-04)National Institutes of Health (Grant 5 TOl GM01555-04)National Aeronautics and Space Administration (Grant NGL 22-009-304