Monitoring of distributed component interactions

We have describe a generic monitoring approach that can be used to enhance the quality of distributed component software. The generality of the approach is achieved through using reflective technology, i.e. CORBA Interceptors, CORBA POA and Java 2 features. A drawback of using interceptors is the fact that their interfaces have not been standardised so far. However, the process of standardisation is ongoing and has recently resulted in an OMG Joint Revised Submission called Portable Interceptors. Although the Java 2 API enables discovery of ‘forks’ in the execution, we find it lacking functionality for the purpose of discovering synchronisation points (e.g., ‘joins’) within multithreaded components

Plasma interactions monitoring system

The plasma environment around the space station is expected to be different from that environment which occurs naturally at these altitudes because of the unprecedented size of the space station, its orbital motion, and its high power distribution system. Although there are models which predict the environment around the station, they do not take into account changes in configuration, changes in the natural and induced environments, nor interactions between the different environments. There will be unique perturbations associated with the space station, which will vary as the space station is being developed. Even after the developed space station has been completed environmental conditions will change as the payloads are changed and as the station systems and materials undergo degradation and modification. Because the space station will be a point of many varied activities the environment will continually undergo perturbations from effluents resulting from operations of the reboost module, EVA, airlock operations, and vacuum venting. The use of the Mobile Service Center will cause disturbances which cannot, at this time, be predicted. Also, the natural environment will be affected by solar flares. In addition, the operations of attached payloads, (e.g., ASTROMAG) themselves will undoubtedly cause perturbations to the ambient environment. Finally, the natural environment will change as a result of natural perturbations such as solar flares and geomagnetic storms

Monitoring Keap1-Nrf2 interactions in single live cells

AbstractThe transcription factor NF-E2 p45-related factor 2 (Nrf2) and its negative regulator Kelch-like ECH associated protein 1 (Keap1) control the expression of nearly 500 genes with diverse cytoprotective functions. Keap1, a substrate adaptor protein for Cullin3/Rbx1 ubiquitin ligase, normally continuously targets Nrf2 for degradation, but loses this ability in response to electrophiles and oxidants (termed inducers). Consequently, Nrf2 accumulates and activates transcription of its downstream target genes. Many inducers are phytochemicals, and cruciferous vegetables represent one of the richest sources of inducer activity among the most commonly used edible plants. Here we summarize the discovery of the isothiocyanate sulforaphane as a potent inducer which reacts with cysteine sensors of Keap1, leading to activation of Nrf2. We then describe the development of a quantitative Förster resonance energy transfer (FRET)-based methodology combined with multiphoton fluorescence lifetime imaging microscopy (FLIM) to investigate the interactions between Keap1 and Nrf2 in single live cells, and the effect of sulforaphane, and other cysteine-reactive inducers, on the dynamics of the Keap1–Nrf2 protein complex. We present the experimental evidence for the “cyclic sequential attachment and regeneration” or “conformation cycling” model of Keap1-mediated Nrf2 degradation. Finally, we discuss the implications of this mode of regulation of Nrf2 for achieving a fine balance under normal physiological conditions, and the consequences and mechanisms of disrupting this balance for tumor biology

Towards self-protecting ubiquitous systems : monitoring trust-based interactions

The requirement for spontaneous interaction in ubiquitous computing creates security issues over and above those present in other areas of computing, deeming traditional approaches ineffective. As a result, to support secure collaborations entities must implement self-protective measures. Trust management is a solution well suited to this task as reasoning about future interactions is based on the outcome of past ones. This requires monitoring of interactions as they take place. Such monitoring also allows us to take corrective action when interactions are proceeding unsatisfactorily. In this vein, we first present a trust-based model of interaction based on event structures. We then describe our ongoing work in the development of a monitor architecture which enables self-protective actions to be carried out at critical points during principal interaction. Finally, we discuss some potential directions for future work

Chloroplast and Cell Imaging at Submicron Resolution by Two-Photon Excitation

Novel, two-photon NIR excitation fluorescence correlation microspectroscopy tests and preliminary results were presented in this article with submicron resolution for concentrated suspensions of functional cells and chloroplast membranes. Related developments of these technique involve applications of Fluorescence Cross-Correlation Spectroscopy (FCCS) detection to monitoring: 
DNA- telomerase interactions, DNA hybridization kinetics, ligand-receptor interactions, and HIV-HBV testing. 
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Monitoring Protein-Ligand Interactions in Human Cells by Real-Time Quantitative In-Cell NMR using a High Cell Density Bioreactor

In-cell NMR is a unique approach to observe the structural and dynamic properties of biological macromolecules at atomic resolution directly in living cells. Protein folding, chemical modifications, and conformational changes induced by ligand binding can be observed. Therefore, this method has great potential in the context of drug development. However, the short lifetime of human cells confined in the NMR spectrometer limits the application range of in-cell NMR. To overcome this issue, NMR bioreactors are employed that can greatly improve the cell sample stability over time and, importantly, enable the real-time recording of in-cell NMR spectra. In this way, the evolution of processes such as ligand penetration and binding to the intracellular protein target can be monitored in real time. Bioreactors are often limited by low cell viability at high cell numbers, which results in a trade-off between the overall sensitivity of the experiment and cell viability. We recently reported an NMR bioreactor that maintains a high number of human cells metabolically active for extended periods of time, up to 72 h. This setup was applied to monitor protein-ligand interactions and protein chemical modification. We also introduced a workflow for quantitative analysis of the real-time NMR data, based on multivariate curve resolution. The method provides concentration profiles of the chemical species present in the cells as a function of time, which can be further analyzed to obtain relevant kinetic parameters. Here we provide a detailed description of the NMR bioreactor setup and its application to monitoring protein-ligand interactions in human cells

Reputation Management and Social Media: How People Monitor Their Identity and Search for Others Online

Based on a survey, analyzes trends in how adults maintain online reputations, including monitoring via search engines and customizing privacy settings on profiles by age group. Examines the role of online information in employment and social interactions

Optical Network Virtualisation using Multi-technology Monitoring and SDN-enabled Optical Transceiver

We introduce the real-time multi-technology transport layer monitoring to facilitate the coordinated virtualisation of optical and Ethernet networks supported by optical virtualise-able transceivers (V-BVT). A monitoring and network resource configuration scheme is proposed to include the hardware monitoring in both Ethernet and Optical layers. The scheme depicts the data and control interactions among multiple network layers under the software defined network (SDN) background, as well as the application that analyses the monitored data obtained from the database. We also present a re-configuration algorithm to adaptively modify the composition of virtual optical networks based on two criteria. The proposed monitoring scheme is experimentally demonstrated with OpenFlow (OF) extensions for a holistic (re-)configuration across both layers in Ethernet switches and V-BVTs