Advances in Microfluidics and Lab-on-a-Chip Technologies

Advances in molecular biology are enabling rapid and efficient analyses for effective intervention in domains such as biology research, infectious disease management, food safety, and biodefense. The emergence of microfluidics and nanotechnologies has enabled both new capabilities and instrument sizes practical for point-of-care. It has also introduced new functionality, enhanced sensitivity, and reduced the time and cost involved in conventional molecular diagnostic techniques. This chapter reviews the application of microfluidics for molecular diagnostics methods such as nucleic acid amplification, next-generation sequencing, high resolution melting analysis, cytogenetics, protein detection and analysis, and cell sorting. We also review microfluidic sample preparation platforms applied to molecular diagnostics and targeted to sample-in, answer-out capabilities

Distributed environmental monitoring

With increasingly ubiquitous use of web-based technologies in society today, autonomous sensor networks represent the future in large-scale information acquisition for applications ranging from environmental monitoring to in vivo sensing. This chapter presents a range of on-going projects with an emphasis on environmental sensing; relevant literature pertaining to sensor networks is reviewed, validated sensing applications are described and the contribution of high-resolution temporal data to better decision-making is discussed

Microfluidic detection and analysis by integration of evanescent wave sensing with thermocapillary actuation

An integrated system capable of microfluidic actuation, detection and sensing is described which combines evanescent wave sensing with thermocapillary manipulation. Liquid droplets or streams transported across the beam path of a planar thin film waveguide, which encapsulates the microheater array, induce attenuation of the propagating waveguide modes. The attenuated signal is used to monitor droplet location, dye concentration in aqueous solutions and reaction kinetics for enzymatic hydrolysis of the sugar X-galactose by beta-galactosidase

Liquid-infiltrated photonic crystals - enhanced light-matter interactions for lab-on-a-chip applications

Optical techniques are finding widespread use in analytical chemistry for chemical and bio-chemical analysis. During the past decade, there has been an increasing emphasis on miniaturization of chemical analysis systems and naturally this has stimulated a large effort in integrating microfluidics and optics in lab-on-a-chip microsystems. This development is partly defining the emerging field of optofluidics. Scaling analysis and experiments have demonstrated the advantage of micro-scale devices over their macroscopic counterparts for a number of chemical applications. However, from an optical point of view, miniaturized devices suffer dramatically from the reduced optical path compared to macroscale experiments, e.g. in a cuvette. Obviously, the reduced optical path complicates the application of optical techniques in lab-on-a-chip systems. In this paper we theoretically discuss how a strongly dispersive photonic crystal environment may be used to enhance the light-matter interactions, thus potentially compensating for the reduced optical path in lab-on-a-chip systems. Combining electromagnetic perturbation theory with full-wave electromagnetic simulations we address the prospects for achieving slow-light enhancement of Beer-Lambert-Bouguer absorption, photonic band-gap based refractometry, and high-Q cavity sensing.Comment: Invited paper accepted for the "Optofluidics" special issue to appear in Microfluidics and Nanofluidics (ed. Prof. David Erickson). 11 pages including 8 figure

National MEMS Technology Roadmap - Markets, Applications and Devices

MEMS teknologiaa on jo pitkään käytetty lukuisien eri laitteiden valmistamiseen. Osa näistä laitteista on ollut markkinoilla jo useita vuosia, kun taas osa on vasta kehitysvaiheessa. Jotta tutkimus ja kehitystyötä osattaisiin jatkossa kohdistaa oikeille painopistealueille, on tärkeää tietää mihin suuntaan kehitys on menossa. Tämä työ on osa kansallista MEMS teknologioiden tiekartta -projektia ja sen tavoitteena oli selvittää MEMS laitteiden kehityksen suuntaa. Työ toteutettiin laajana kirjallisuustutkimuksena. Lisäksi tulosten tueksi haastateltiin asiantuntijoita Suomen MEMS teollisuudesta. Työssä tarkasteltiin lukuisia jo markkinoilta löytyviä ja vasta kehitteillä olevia MEMS laitteita ja analysoitiin niitä sekä teknisestä että kaupallisesta näkökulmasta. Tutkimuksen perusteella kävi ilmi, että MEMS markkinat ovat pitkään muodostuneet vakiintuneista laitteista kuten mustesuihkupäistä, kiihtyvyysantureista, paineantureista sekä RF suotimista. Lisäksi mikrofonit, gyroskoopit ja optiset laitteet ovat olleet kaupallisesti saatavilla jo pitkään. Markkinat ovat hiljattain alkaneet tehdä tilaa myös uusille MEMS laitteille, joita tulee ulos nopeaa vauhtia. Viimeisimpänä markkinoille tulleita laitteita ovat erilaiset mikrofluidistiikka laitteet, mikrobolometrit sekä yhdistelmäanturit. Pian kaupallisesti saatavia laitteita ovat magnetometrit, automaattitarkennuslaitteet sekä MEMS oskillaattorit. Näiden laitteiden lisäksi kehitteillä on monia uusia MEMS laitteita, jotka saattavat tarjota merkittäviä mahdollisuuksia tulevaisuudessa. Kehitteillä olevia laitteita ovat erilaiset lääketieteelliset laitteet, atomikellot, mikrojäähdyttimet, mikrokaiuttimet, energiantuottolaitteet sekä RFID-laitteet. Kaikki kehitteillä olevista laitteista eivät välttämättä tule menestymään kaupallisesti, mutta jatkuva tutkimustyö osoittaa, että monilla MEMS laitteilla on potentiaalia useissa eri sovelluksissa. Markkinanäkökulmasta tarkasteltuna suurin potentiaali piilee kuluttajaelektroniikka markkinoilla. Muita tulevaisuuden kannalta potentiaalisia markkinoita ovat lääketieteelliset ja teollisuusmarkkinat. Tutkimus osoitti että MEMS laitteiden tutkimukseen ja kehitykseen liittyy monia potentiaalisia painopistealueita tulevaisuudessa. Käyttömahdollisuuksien parantamiseksi monet jo vakiintuneet laitteet kaipaavat vielä parannuksia. Toisaalta, jo olemassa olevia laitteita voidaan hyödyntää uusissa sovelluksissa. Lisäksi monet uusista ja kehitteillä olevista MEMS laitteista vaativat vielä kehitystyötä.MEMS technology has long been applied to the fabrication of various devices from which some have already been in use for several years, whereas others are still under development. In order to find future focus areas in research and development activities in the industry, it is important to know where the development is going. This thesis was conducted as a part of National MEMS technology roadmap, and it aimed for determining the evolution of MEMS devices. The work was conducted as an extensive literature review. In addition, experts from the Finnish MEMS industry were interviewed in order obtain a broader insight to the results. In this thesis various existing and emerging MEMS devices were reviewed and analyzed from technological and commercial perspectives. The study showed that the MEMS market has long been composed of established devices, such as inkjet print-heads, pressure sensors, accelerometers and RF filters. Also gyroscopes, microphones and optical MEMS devices have already been on the market for a long time. Lately, many new devices have started to find their place in the markets. The most recently introduced commercial devices include microfluidic devices, micro bolometers, and combo sensors. There are also a few devices including magnetometers, MEMS oscillators, and auto-focus devices that are currently crossing the gap from R&D to commercialization. In addition to the already available devices, many new MEMS devices are under development, and might offer significant opportunities in the future. These emerging devices include various bioMEMS devices, atomic clocks, micro-coolers, micro speakers, power MEMS devices, and RFID devices. All of the emerging devices might not find commercial success, but the constant stream shows, that there are numerous applications, where MEMS devices could be applied in. From a market point of view, the greatest potential in the future lies in consumer electronics market. Other highly potential markets include medical and industrial markets. The results of the thesis indicate that there are many potential focus areas in the future related to MEMS devices, including improvements of the existing devices in order to gain better utilization, application of the existing devices in new areas, and development work among the emerging devices

Microfluidic platform for bilayer experimatation from a research tooltowards drug screening

The aim of this thesis, which is the development of a microfluidic platform for bilayer experimentation with the potential for drug screening on ion channels, is introduced in this chapter. After a short presentation of the field of drug screening, an outline of this thesis is given, together with a brief summary of the different chapters