Direct comparison of distinct naive pluripotent states in human embryonic stem cells

Until recently, human embryonic stem cells (hESCs) were shown to exist in a state of primed pluripotency, while mouse embryonic stem cells (mESCs) display a naive or primed pluripotent state. Here we show the rapid conversion of in-house-derived primed hESCs on mouse embryonic feeder layer (MEF) to a naive state within 5-6 days in naive conversion media (NCM-MEF), 6-10 days in naive human stem cell media (NHSM-MEF) and 14-20 days using the reverse-toggle protocol (RT-MEF). We further observe enhanced unbiased lineage-specific differentiation potential of naive hESCs converted in NCM-MEF, however, all naive hESCs fail to differentiate towards functional cell types. RNA-seq analysis reveals a divergent role of PI3K/AKT/mTORC signalling, specifically of the mTORC2 subunit, in the different naive hESCs. Overall, we demonstrate a direct evaluation of several naive culture conditions performed in the same laboratory, thereby contributing to an unbiased, more in-depth understanding of different naive hESCs

Cosmological Constraints on the Modified Entropic Force Model

Very recently, Verlinde considered a theory in which space is emergent through a holographic scenario, and proposed that gravity can be explained as an entropic force caused by changes in the information associated with the positions of material bodies. Then, motivated by the Debye model in thermodynamics which is very successful in very low temperatures, Gao modified the entropic force scenario. The modified entropic force (MEF) model is in fact a modified gravity model, and the universe can be accelerated without dark energy. In the present work, we consider the cosmological constraints on the MEF model, and successfully constrain the model parameters to a narrow range. We also discuss many other issues of the MEF model. In particular, we clearly reveal the implicit root to accelerate the universe in the MEF model.Comment: 16 pages, 7 figures, revtex4; v2: discussions added, Phys. Lett. B in press; v3: published versio

Strong interfacial exchange field in the graphene/EuS heterostructure

Exploiting 2D materials for spintronic applications can potentially realize next-generation devices featuring low-power consumption and quantum operation capability. The magnetic exchange field (MEF) induced by an adjacent magnetic insulator enables efficient control of local spin generation and spin modulation in 2D devices without compromising the delicate material structures. Using graphene as a prototypical 2D system, we demonstrate that its coupling to the model magnetic insulator (EuS) produces a substantial MEF (> 14 T) with potential to reach hundreds of Tesla, which leads to orders-of-magnitude enhancement in the spin signal originated from Zeeman spin-Hall effect. Furthermore, the new ferromagnetic ground state of Dirac electrons resulting from the strong MEF may give rise to quantized spin-polarized edge transport. The MEF effect shown in our graphene/EuS devices therefore provides a key functionality for future spin logic and memory devices based on emerging 2D materials in classical and quantum information processing

Modeling heat transfer in dilute two-phase flows using the Mesoscopic Eulerian Formalism

In dilute two-phase flows, accurate prediction of the temperature of the dis- persed phase can be of paramount importance. Indeed, processes such as evaporation or chemical reactions are strongly non-linear functions of heat transfer between the carrier and dispersed phases. This study is devoted to the validation of an Eulerian description of the dispersed phase –the Meso- scopic Eulerian Formalism (MEF)– in the case of non-isothermal flows. Di- rect numerical simulations using the MEF are compared to a reference La- grangian simulation for a two-dimensional non-isothermal turbulent jet laden with solid particles. The objectives of this paper are (1) to study the influ- ence of the thermal inertia of particles on their temperature distribution and (2) conduct an a posteriori validation of the MEF, which was recently ex- tended to non-isothermal flows. The focus is on the influence of additional terms in the MEF governing equations, namely heat fluxes arising from the Random Uncorrelated Motion (RUM). Results show that mean and rms of particle temperature are strongly dependent of the thermal Stokes number. The mean temperature is satisfactorily predicted by the MEF, comparing to the Lagrangian reference. Under the conditions of the present study, the RUM heat fluxes have a marginal influence on the mean particle tempera- ture. However, a significant impact was observed on the magnitude of particle temperature fluctuations. Neglecting the RUM heat fluxes leads to erroneous results while the Lagrangian statistics are recovered when it is accounted for in the regimes of low to moderate thermal Stokes number

MEF Üniversitesi Akademik / Kurumsal Arşiv Sistemi Yönergesi

MEF Üniversitesi Akademik / Kurumsal Arşiv Yönergesi, öğretim elemanlarının araştırmalarının ve entellektüel ürünlerinin erişilebilirliğini, görünürlüğünü, kullanımını ve etkisini artırmak; araştırmalardan üretilen yayınları daha etkin yönetebilmek ve kamu kaynaklarıyla desteklenen araştırmalardan üretilen yayınlara serbestçe erişimi desteklemek amacıyla geliştirilmiştir. Bu yönerge, MEF Üniversitesi adresli bilimsel çalışmaların, MEF Üniversitesi Akademik/Kurmsal Sistemin'de arşivlenerek bilim dünyasının hizmetine sunulmasına ilişkin usul ve esasları düzenler

Multi-element fiber technology for space-division multiplexing applications

A novel technological approach to space division multiplexing (SDM) based on the use of multiple individual fibers embedded in a common polymer coating material is presented, which is referred to as Multi-Element Fiber (MEF). The approach ensures ultralow crosstalk between spatial channels and allows for cost-effective ways of realizing multi-spatial channel amplification and signal multiplexing/demultiplexing. Both the fabrication and characterization of a passive 3-element MEF for data transmission, and an active 5-element erbium/ytterbium doped MEF for cladding-pumped optical amplification that uses one of the elements as an integrated pump delivery fiber is reported. Finally, both components were combined to emulate an optical fiber network comprising SDM transmission lines and amplifiers, and illustrate the compatibility of the approach with existing installed single-mode WDM fiber systems

Nanowire-Intensified MEF in Hybrid Polymer-Plasmonic Electrospun Filaments

Hybrid polymer-plasmonic nanostructures might combine high enhancement of localized fields from metal nanoparticles with light confinement and long-range transport in subwavelength dielectric structures. Here we report on the complex behavior of fluorophores coupling to Au nanoparticles within polymer nanowires, which features localized metal-enhanced fluorescence (MEF) with unique characteristics compared to conventional structures. The intensification effect when the particle is placed in the organic filaments is remarkably higher with respect to thin films of comparable thickness, thus highlighting a specific, nanowire-related enhancement of MEF effects. A dependence on the confinement volume in the dielectric nanowire is also evidenced, with MEF significantly increasing upon reducing the wire diameter. These findings are rationalized by finite element simulations, predicting a position-dependent enhancement of the quantum yield of fluorophores embedded in the fibers. Calculation of the ensemble-averaged fluorescence enhancement unveils the possibility of strongly enhancing the overall emission intensity for structures with size twice the diameter of the embedded metal particles. These new, hybrid fluorescent systems with localized enhanced emission, as well as the general Nanowire-Intensified MEF effect associated to them, are highly relevant for developing nanoscale light-emitting devices with high efficiency and inter-coupled through nanofiber networks, highly sensitive optical sensors, and novel laser architectures.Comment: 29 pages, 12 figures, Small (2018

Testing microelectronic biofluidic systems

According to the 2005 International Technology Roadmap for Semiconductors, the integration of emerging nondigital CMOS technologies will require radically different test methods, posing a major challenge for designers and test engineers. One such technology is microelectronic fluidic (MEF) arrays, which have rapidly gained importance in many biological, pharmaceutical, and industrial applications. The advantages of these systems, such as operation speed, use of very small amounts of liquid, on-board droplet detection, signal conditioning, and vast digital signal processing, make them very promising. However, testable design of these devices in a mass-production environment is still in its infancy, hampering their low-cost introduction to the market. This article describes analog and digital MEF design and testing method