Analyzing large-scale DNA Sequences on Multi-core Architectures

Rapid analysis of DNA sequences is important in preventing the evolution of different viruses and bacteria during an early phase, early diagnosis of genetic predispositions to certain diseases (cancer, cardiovascular diseases), and in DNA forensics. However, real-world DNA sequences may comprise several Gigabytes and the process of DNA analysis demands adequate computational resources to be completed within a reasonable time. In this paper we present a scalable approach for parallel DNA analysis that is based on Finite Automata, and which is suitable for analyzing very large DNA segments. We evaluate our approach for real-world DNA segments of mouse (2.7GB), cat (2.4GB), dog (2.4GB), chicken (1GB), human (3.2GB) and turkey (0.2GB). Experimental results on a dual-socket shared-memory system with 24 physical cores show speed-ups of up to 17.6x. Our approach is up to 3x faster than a pattern-based parallel approach that uses the RE2 library.Comment: The 18th IEEE International Conference on Computational Science and Engineering (CSE 2015), Porto, Portugal, 20 - 23 October 201

DNA COMPRESSED AND SEQUENCE SEARCHING ON MULTICORE

Abstract : One of the used of string matching is to search DNA sequence in the DNA database. This simple operation can be done in hours or days, because the huge size of DNA sequence database. On the other hand, the potential of multicore for DNA sequence searching is not fully explored due to the difficulty of multicore programming. This paper evaluates several key string matching algorithms using a comprehensive simulation framework. Starting from decoding compressed DNA sequence and instructions processor profiling, the framework constructs task graphs for string matching algorithms. Then task graphs are mapped onto multicore. The mapping technique is based on a random algorithm result in a high mapping quality. The key feature of this paper is that entire processes are automated and it requires users little understanding of the complexity of algorithms and multicore hardware architecture. DNA compressed can save up to 75% space and our framework can be as a guidance to utilize multicore for searching DNA pattern

MPI-dot2dot: A Parallel Tool to Find DNA Tandem Repeats on Multicore Clusters

Financiado para publicación en acceso aberto: Universidade da Coruña/CISUG[Abstract] Tandem Repeats (TRs) are segments that occur several times in a DNA sequence, and each copy is adjacent to other. In the last few years, TRs have gained significant attention as they are thought to be related with certain human diseases. Therefore, identifying and classifying TRs have become a highly important task in bioinformatics in order to analyze their disorders and relationships with illnesses. Dot2dot, a tool recently developed to find TRs, provides more accurate results than the previous state-of-the-art, but it requires a long execution time even when using multiple threads. This work presents MPI-dot2dot, a novel version of this tool that combines MPI and OpenMP so that it can be executed in a cluster of multicore nodes and thus reduces its execution time. The performance of this new parallel implementation has been tested using different real datasets. Depending on the characteristics of the input genomes, it is able to obtain the same biological results as Dot2dot but more than 100 times faster on a 16-node multicore cluster (384 cores). MPI-dot2dot is publicly available to download from https://sourceforge.net/projects/mpi-dot2dot.This work was supported by the Ministry of Science and Innovation of Spain (PID2019-104184RB-I00 / AEI / 10.13039/501100011033), and by Xunta de Galicia and FEDER funds (Centro de Investigación de Galicia accreditation 2019-2022 and Consolidation Program of Competitive Reference Groups, under Grants ED431G 2019/01 and ED431C 2021/30, respectively). The authors would like to thank the Galician Supercomputing Center (CESGA) for providing access to the Finis Terrae II supercomputer. Open Access funding provided thanks to the CRUE-CSIC agreement with Springer NatureXunta de Galicia; ED431G 2019/01Xunta de Galicia; ED431C 2021/3

PLAST: parallel local alignment search tool for database comparison

Background: Sequence similarity searching is an important and challenging task in molecular biology and next-generation sequencing should further strengthen the need for faster algorithms to process such vast amounts of data. At the same time, the internal architecture of current microprocessors is tending towards more parallelism, leading to the use of chips with two, four and more cores integrated on the same die. The main purpose of this work was to design an effective algorithm to fit with the parallel capabilities of modern microprocessors. Results: A parallel algorithm for comparing large genomic banks and targeting middle-range computers has been developed and implemented in PLAST software. The algorithm exploits two key parallel features of existing and future microprocessors: the SIMD programming model (SSE instruction set) and the multithreading concept (multicore). Compared to multithreaded BLAST software, tests performed on an 8-processor server have shown speedup ranging from 3 to 6 with a similar level of accuracy. Conclusions: A parallel algorithmic approach driven by the knowledge of the internal microprocessor architecture allows significant speedup to be obtained while preserving standard sensitivity for similarity search problems.

Automated Genome-Wide Protein Domain Exploration

Exploiting the exponentially growing genomics and proteomics data requires high quality, automated analysis. Protein domain modeling is a key area of molecular biology as it unravels the mysteries of evolution, protein structures, and protein functions. A plethora of sequences exist in protein databases with incomplete domain knowledge. Hence this research explores automated bioinformatics tools for faster protein domain analysis. Automated tool chains described in this dissertation generate new protein domain models thus enabling more effective genome-wide protein domain analysis. To validate the new tool chains, the Shewanella oneidensis and Escherichia coli genomes were processed, resulting in a new peptide domain database, detection of poor domain models, and identification of likely new domains. The automated tool chains will require months or years to model a small genome when executing on a single workstation. Therefore the dissertation investigates approaches with grid computing and parallel processing to significantly accelerate these bioinformatics tool chains

Comparison of Communication/Synchronization Models in Parallel Programming on Multi-Core Cluster

Taking into account the increase in use of the multi-core cluster architecture, in this paper we analyze the use of the various communication models (message passing, shared memory, their combination) to efficiently exploit the power of the architecture. Smith-Waterman algorithm, whose parallelization is based on a pipeline scheme due to problem data dependence, is used as test case to determine the similarity degree of two DNA sequences. Finally, future research lines are mentioned, aimed at optimizing the use of memory levels in the architecture.Red de Universidades con Carreras en Informática (RedUNCI

Benchmarking a many-core neuromorphic platform with an MPI-based DNA sequence matching algorithm

SpiNNaker is a neuromorphic globally asynchronous locally synchronous (GALS)multi-core architecture designed for simulating a spiking neural network (SNN) in real-time. Several studies have shown that neuromorphic platforms allow flexible and efficient simulations of SNN by exploiting the efficient communication infrastructure optimised for transmitting small packets across the many cores of the platform. However, the effectiveness of neuromorphic platforms in executing massively parallel general-purpose algorithms, while promising, is still to be explored. In this paper, we present an implementation of a parallel DNA sequence matching algorithm implemented by using the MPI programming paradigm ported to the SpiNNaker platform. In our implementation, all cores available in the board are configured for executing in parallel an optimised version of the Boyer-Moore (BM) algorithm. Exploiting this application, we benchmarked the SpiNNaker platform in terms of scalability and synchronisation latency. Experimental results indicate that the SpiNNaker parallel architecture allows a linear performance increase with the number of used cores and shows better scalability compared to a general-purpose multi-core computing platform

About BIRDS project (Bioinformatics and Information Retrieval Data Structures Analysis and Design)

BIRDS stands for "Bioinformatics and Information Retrieval Data Structures analysis and design" and is a 4-year project (2016--2019) that has received funding from the European Union's Horizon 2020 research and innovation programme under the Marie Sklodowska-Curie grant agreement No 690941. The overall goal of BIRDS is to establish a long term international network involving leading researchers in the development of efficient data structures in the fields of Bioinformatics and Information Retrieval, to strengthen the partnership through the exchange of knowledge and expertise, and to develop integrated approaches to improve current approaches in both fields. The research will address challenges in storing, processing, indexing, searching and navigating genome-scale data by designing new algorithms and data structures for sequence analysis, networks representation or compressing and indexing repetitive data. BIRDS project is carried out by 7 research institutions from Australia (University of Melbourne), Chile (University of Chile and University of Concepci\'on), Finland (University of Helsinki), Japan (Kyushu University), Portugal (Instituto de Engenharia de Sistemas e Computadores, Investiga\c{c}\~ao e Desenvolvimento em Lisboa, INESC-ID), and Spain (University of A Coru\~na), and a Spanish SME (Enxenio S.L.). It is coordinated by the University of A Coru\~na (Spain).Comment: This research has received funding from the European Union's Horizon 2020 research and innovation programme under the Marie Sklodowska-Curie Actions H2020-MSCA-RISE-2015 BIRDS GA No. 690941. CERI 201