The potential for using remote sensing to quantify stress in and predict yield of sugarcane (Saccharum spp. hybrid)

Thesis (Ph.D.)-University of KwaZulu-Natal, Pietermaritzburg, 2010

Uumanned Aerial Vehicle Data Analysis For High-throughput Plant Phenotyping

The continuing population is placing unprecedented demands on worldwide crop yield production and quality. Improving genomic selection for breeding process is one essential aspect for solving this dilemma. Benefitted from the advances in high-throughput genotyping, researchers already gained better understanding of genetic traits. However, given the comparatively lower efficiency in current phenotyping technique, the significance of phenotypic traits has still not fully exploited in genomic selection. Therefore, improving HTPP efficiency has become an urgent task for researchers. As one of the platforms utilized for collecting HTPP data, unmanned aerial vehicle (UAV) allows high quality data to be collected within short time and by less labor. There are currently many options for customized UAV system on market; however, data analysis efficiency is still one limitation for the fully implementation of HTPP. To this end, the focus of this program was data analysis of UAV acquired data. The specific objectives were two-fold, one was to investigate statistical correlations between UAV derived phenotypic traits and manually measured sorghum biomass, nitrogen and chlorophyll content. Another was to conduct variable selection on the phenotypic parameters calculated from UAV derived vegetation index (VI) and plant height maps, aiming to find out the principal parameters that contribute most in explaining winter wheat grain yield. Corresponding, two studies were carried out. Good correlations between UAV-derived VI/plant height and sorghum biomass/nitrogen/chlorophyll in the first study suggested that UAV-based HTPP has great potential in facilitating genetic improvement. For the second study, variable selection results from the single-year data showed that plant height related parameters, especially from later season, contributed more in explaining grain yield. Advisor: Yeyin Sh

Remote Sensing for Precision Nitrogen Management

This book focuses on the fundamental and applied research of the non-destructive estimation and diagnosis of crop leaf and plant nitrogen status and in-season nitrogen management strategies based on leaf sensors, proximal canopy sensors, unmanned aerial vehicle remote sensing, manned aerial remote sensing and satellite remote sensing technologies. Statistical and machine learning methods are used to predict plant-nitrogen-related parameters with sensor data or sensor data together with soil, landscape, weather and/or management information. Different sensing technologies or different modelling approaches are compared and evaluated. Strategies are developed to use crop sensing data for in-season nitrogen recommendations to improve nitrogen use efficiency and protect the environment

Principal variable selection to explain grain yield variation in winter wheat from features extracted from UAV imagery

Background: Automated phenotyping technologies are continually advancing the breeding process. However, collecting various secondary traits throughout the growing season and processing massive amounts of data still take great efforts and time. Selecting a minimum number of secondary traits that have the maximum predictive power has the potential to reduce phenotyping efforts. The objective of this study was to select principal features extracted from UAV imagery and critical growth stages that contributed the most in explaining winter wheat grain yield. Five dates of multispectral images and seven dates of RGB images were collected by a UAV system during the spring growing season in 2018. Two classes of features (variables), totaling to 172 variables, were extracted for each plot from the vegetation index and plant height maps, including pixel statistics and dynamic growth rates. A parametric algorithm, LASSO regression (the least angle and shrinkage selection operator), and a non-parametric algorithm, random forest, were applied for variable selection. The regression coefficients estimated by LASSO and the permutation importance scores provided by random forest were used to determine the ten most important variables influencing grain yield from each algorithm. Results: Both selection algorithms assigned the highest importance score to the variables related with plant height around the grain filling stage. Some vegetation indices related variables were also selected by the algorithms mainly at earlier to mid growth stages and during the senescence. Compared with the yield prediction using all 172 variables derived from measured phenotypes, using the selected variables performed comparable or even better. We also noticed that the prediction accuracy on the adapted NE lines (r = 0.58–0.81) was higher than the other lines (r = 0.21–0.59) included in this study with different genetic backgrounds. Conclusions: With the ultra-high resolution plot imagery obtained by the UAS-based phenotyping we are now able to derive more features, such as the variation of plant height or vegetation indices within a plot other than just an averaged number, that are potentially very useful for the breeding purpose. However, too many features or variables can be derived in this way. The promising results from this study suggests that the selected set from those variables can have comparable prediction accuracies on the grain yield prediction than the full set of them but possibly resulting in a better allocation of efforts and resources on phenotypic data collection and processing

Quantifying Vegetation Biophysical Variables from Imaging Spectroscopy Data: A Review on Retrieval Methods

An unprecedented spectroscopic data stream will soon become available with forthcoming Earth-observing satellite missions equipped with imaging spectroradiometers. This data stream will open up a vast array of opportunities to quantify a diversity of biochemical and structural vegetation properties. The processing requirements for such large data streams require reliable retrieval techniques enabling the spatiotemporally explicit quantification of biophysical variables. With the aim of preparing for this new era of Earth observation, this review summarizes the state-of-the-art retrieval methods that have been applied in experimental imaging spectroscopy studies inferring all kinds of vegetation biophysical variables. Identified retrieval methods are categorized into: (1) parametric regression, including vegetation indices, shape indices and spectral transformations; (2) nonparametric regression, including linear and nonlinear machine learning regression algorithms; (3) physically based, including inversion of radiative transfer models (RTMs) using numerical optimization and look-up table approaches; and (4) hybrid regression methods, which combine RTM simulations with machine learning regression methods. For each of these categories, an overview of widely applied methods with application to mapping vegetation properties is given. In view of processing imaging spectroscopy data, a critical aspect involves the challenge of dealing with spectral multicollinearity. The ability to provide robust estimates, retrieval uncertainties and acceptable retrieval processing speed are other important aspects in view of operational processing. Recommendations towards new-generation spectroscopy-based processing chains for operational production of biophysical variables are given

High-throughput phenotyping of plant leaf morphological, physiological, and biochemical traits on multiple scales using optical sensing

Acquisition of plant phenotypic information facilitates plant breeding, sheds light on gene action, and can be applied to optimize the quality of agricultural and forestry products. Because leaves often show the fastest responses to external environmental stimuli, leaf phenotypic traits are indicators of plant growth, health, and stress levels. Combination of new imaging sensors, image processing, and data analytics permits measurement over the full life span of plants at high temporal resolution and at several organizational levels from organs to individual plants to field populations of plants. We review the optical sensors and associated data analytics used for measuring morphological, physiological, and biochemical traits of plant leaves on multiple scales. We summarize the characteristics, advantages and limitations of optical sensing and data-processing methods applied in various plant phenotyping scenarios. Finally, we discuss the future prospects of plant leaf phenotyping research. This review aims to help researchers choose appropriate optical sensors and data processing methods to acquire plant leaf phenotypes rapidly, accurately, and cost-effectively

Wheat yellow rust monitoring by learning from multispectral UAV aerial imagery

The use of a low-cost five-band multispectral camera (RedEdge, MicaSense, USA) and a low-altitude airborne platform is investigated for the detection of plant stress caused by yellow rust disease in winter wheat for sustainable agriculture. The research is mainly focused on: (i) determining whether or not healthy and yellow rust infected wheat plants can be discriminated; (ii) selecting spectral band and Spectral Vegetation Index (SVI) with a strong discriminating capability; (iii) developing a low-cost yellow rust monitoring system for use at farmland scales. An experiment was carefully designed by infecting winter wheat with different levels of yellow rust inoculum, where aerial multispectral images under different developmental stages of yellow rust were captured by an Unmanned Aerial Vehicle at an altitude of 16–24m with a ground resolution of 1–1.5cm/pixel. An automated yellow rust detection system is developed by learning (via random forest classifier) from labelled UAV aerial multispectral imagery. Experimental results indicate that: (i) good classification performance (with an average Precision, Recall and Accuracy of 89.2%, 89.4% and 89.3%) was achieved by the developed yellow rust monitoring at a diseased stage (45 days after inoculation); (ii) the top three SVIs for separating healthy and yellow rust infected wheat plants are RVI, NDVI and OSAVI; while the top two spectral bands are NIR and Red. The learnt system was also applied to the whole farmland of interest with a promising monitoring result. It is anticipated that this study by seamlessly integrating low-cost multispectral camera, low-altitude UAV platform and machine learning techniques paves the way for yellow rust monitoring at farmland scales

Mehitamata õhusõiduki rakendamine põllukultuuride saagikuse ja maa harimisviiside tuvastamisel

A Thesis for applying for the degree of Doctor of Philosophy in Environmental Protection.Väitekiri filosoofiadoktori kraadi taotlemiseks keskkonnakaitse erialal.This thesis aims to examine how machine learning (ML) technologies have aided significant advancements in image analysis in the area of precision agriculture. These multimodal computing technologies extend the use of machine learning to a broader spectrum of data collecting and selection for the advancement of agricultural practices (Nawar et al., 2017) These techniques will assist complicated cropping systems with more informed decisions with less human intervention, and provide a scalable framework for incorporating expert knowledge of the PA system. (Chlingaryan et al., 2018). Complexity, on the other hand, can be seen as a disadvantage in crop trials, as machine learning models require training/testing databases, limited areas with insignificant sampling sizes, time and space-specificity, and environmental factor interventions, all of which complicate parameter selection and make using a single empirical model for an entire region impractical. During the early stages of writing this thesis, we used a relatively traditional machine learning method to address the regression problem of crop yield and biomass prediction [(i.e., random forest regression (RFR), support vector regression (SVR), and artificial neural network (ANN)] to predicted dry matter (DM) yields of red clover. It obtained favourable results, however, the choosing of hyperparameters, the lengthy algorithms selection process, data cleaning, and redundant collinearity issues significantly limited the way of the machine learning application. We will further discuss the recent trend of automated machine learning (AutoML) that has been driving further significant technological innovation in the application of artificial intelligence from its automated algorithm selection and hyperparameter optimization of the deployable pipeline model for unravelling substance problems. However, a present knowledge gap exists in the integration of machine learning (ML) technology with unmanned aerial systems (UAS) and hyperspectral-based imaging data categorization and regression applications. In this thesis, we explored a state-of-the-art (SOTA) and entirely open-source AutoML framework, Auto-sklearn, which was built on one of the most frequently used machine learning systems, Scikit-learn. It was integrated with two unique AutoML visualization tools to examine the recognition and acceptance of multispectral vegetation indices (VI) data collected from UAS and hyperspectral narrow-band VIs across a varied spectrum of agricultural management practices (AMP). These procedures incorporate soil tillage method (STM), cultivation method (CM), and manure application (MA), and are classified as four-crop combination fields (i.e., red clover-grass mixture, spring wheat, pea-oat mixture, and spring barley). Additionally, they have not been thoroughly evaluated and lack characteristics that are accessible in agriculture remote sensing applications. This thesis further explores the existing gaps in the knowledge base for several critical crop categories and cultivation management methods referring to biomass and yield analysis, as well as to gain a better understanding of the potential for remotely sensed solutions to field-based and multifunctional platforms to meet precision agriculture demands. To overcome these knowledge gaps, this research introduces a rapid, non-destructive, and low-cost framework for field-based biomass and grain yield modelling, as well as the identification of agricultural management practices. The results may aid agronomists and farmers in establishing more accurate agricultural methods and in monitoring environmental conditions more effectively.Doktoritöö eesmärk oli uurida, kuidas masinõppe (MÕ) tehnoloogiad võimaldavad edusamme täppispõllumajanduse valdkonna pildianalüüsis. Multimodaalsed arvutustehnoloogiad laiendavad masinõppe kasutamist põllumajanduses andmete kogumisel ja valimisel (Nawar et al., 2017). Selline täpsemal informatsioonil põhinev tehnoloogia võimaldab keerukate viljelussüsteemide puhul teha otsuseid inimese vähema sekkumisega, ja loob skaleeritava raamistiku täppispõllumajanduse jaoks (Chlingaryan et al., 2018). Põllukultuuride katsete korral on komplekssete masinõppemudelite kasutamine keerukas, sest alad on piiratud ning valimi suurus ei ole piisav; vaja on testandmebaase, kindlaid aja- ja ruumitingimusi ning keskkonnategureid. See komplitseerib parameetrite valikut ning muudab ebapraktiliseks ühe empiirilise mudeli kasutamise terves piirkonnas. Siinse uurimuse algetapis rakendati suhteliselt traditsioonilist masinõppemeetodit, et lahendada saagikuse ja biomassi prognoosimise regressiooniprobleem (otsustusmetsa regression, tugivektori regressioon ja tehisnärvivõrk) punase ristiku prognoositava kuivaine saagikuse suhtes. Saadi sobivaid tulemusi, kuid hüperparameetrite valimine, pikk algoritmide valimisprotsess, andmete puhastamine ja kollineaarsusprobleemid takistasid masinõpet oluliselt. Automatiseeritud masinõppe (AMÕ) uusimate suundumustena rakendatakse tehisintellekti, et lahendada põhiprobleemid automatiseeritud algoritmi valiku ja rakendatava pipeline-mudeli hüperparameetrite optimeerimise abil. Seni napib teadmisi MÕ tehnoloogia integreerimiseks mehitamata õhusõidukite ning hüperspektripõhiste pildiandmete kategoriseerimise ja regressioonirakendustega. Väitekirjas uuriti nüüdisaegset ja avatud lähtekoodiga AMÕ tehnoloogiat Auto-sklearn, mis on ühe enimkasutatava masinõppesüsteemi Scikit-learn edasiarendus. Süsteemiga liideti kaks unikaalset AMÕ visualiseerimisrakendust, et uurida mehitamata õhusõidukiga kogutud andmete multispektraalsete taimkatteindeksite ja hüperspektraalsete kitsaribaandmete taimkatteindeksite tuvastamist ja rakendamist põllumajanduses. Neid võtteid kasutatakse mullaharimisel, kultiveerimisel ja sõnnikuga väetamisel nelja kultuuriga põldudel (punase ristiku rohusegu, suvinisu, herne-kaera segu, suvioder). Neid ei ole põhjalikult hinnatud, samuti ei hõlma need omadusi, mida kasutatatakse põllumajanduses kaugseire rakendustes. Uurimus käsitleb biomassi ja saagikuse seni uurimata analüüsivõimalusi oluliste põllukultuuride ja viljelusmeetodite näitel. Hinnatakse ka kaugseirelahenduste potentsiaali põllupõhiste ja multifunktsionaalsete platvormide kasutamisel täppispõllumajanduses. Uurimus tutvustab kiiret, keskkonna suhtes kahjutut ja mõõduka hinnaga tehnoloogiat põllupõhise biomassi ja teraviljasaagi modelleerimiseks, et leida sobiv viljelusviis. Töö tulemused võimaldavad põllumajandustootjatel ja agronoomidel tõhusamalt valida põllundustehnoloogiaid ning arvestada täpsemalt keskkonnatingimustega.Publication of this thesis is supported by the Estonian University of Life Scieces and by the Doctoral School of Earth Sciences and Ecology created under the auspices of the European Social Fund

Unlocking the benefits of spaceborne imaging spectroscopy for sustainable agriculture

With the Environmental Mapping and Analysis Program (EnMAP) mission, launched on April 1st 2022, new opportunities unfold for precision farming and agricultural monitoring. The recurring acquisition of spectrometric imagery from space, contiguously resolving the electromagnetic spectrum in the optical domain (400—2500 nm) within close narrow bands, provides unprecedented data about the interaction of radiation with biophysical and biochemical crop constituents. These interactions manifest in spectral reflectance, carrying important information about crop status and health. This information may be incorporated in agricultural management systems to support necessary efforts to maximize yields against the backdrop of an increased food demand by a growing world population. At the same time, it enables the effective optimization of fertilization and pest control to minimize environmental impacts of agriculture. Deriving biophysical and biochemical crop traits from hyperspectral reflectance thereby always relies on a model. These models are categorized into (1) parametric, (2) nonparametric, (3) physically-based, and (4) hybrid retrieval schemes. Parametric methods define an explicit parameterized expression, relating a number of spectral bands or derivates thereof with a crop trait of interest. Nonparametric methods comprise linear techniques, such as principal component analysis (PCA) which addresses collinearity issues between adjacent bands and enables compression of full spectral information into dimensionality reduced, maximal informative principal components (PCs). Nonparametric nonlinear methods, i.e., machine learning (ML) algorithms apply nonlinear transformations to imaging spectroscopy data and are therefore capable of capturing nonlinear relationships within the contained spectral features. Physically-based methods represent an umbrella term for radiative transfer models (RTMs) and related retrieval schemes, such as look-up-table (LUT) inversion. A simple, easily invertible and specific RTM is the Beer-Lambert law which may be used to directly infer plant water content. The most widely used general and invertible RTM is the one-dimensional canopy RTM PROSAIL, which is coupling the Leaf Optical Properties Spectra model PROSPECT and the canopy reflectance model 4SAIL: Scattering by Arbitrarily Inclined Leaves. Hybrid methods make use of synthetic data sets created by RTMs to calibrate parametric methods or to train nonparametric ML algorithms. Due to the ill-posed nature of RTM inversion, potentially unrealistic and redundant samples in a LUT need to be removed by either implementing physiological constraints or by applying active learning (AL) heuristics. This cumulative thesis presents three different hybrid approaches, demonstrated within three scientific research papers, to derive agricultural relevant crop traits from spectrometric imagery. In paper I the Beer-Lambert law is applied to directly infer the thickness of the optically active water layer (i.e., EWT) from the liquid water absorption feature at 970 nm. The model is calibrated with 50,000 PROSPECT spectra and validated over in situ data. Due to separate water content measurements of leaves, stalks, and fruits during the Munich-North-Isar (MNI) campaigns, findings indicate that depending on the crop type and its structure, different parts of the canopy are observed with optical sensors. For winter wheat, correlation between measured and modelled water content was most promising for ears and leaves, reaching coefficients of determination (R2) up to 0.72 and relative RMSE (rRMSE) of 26%, and in the case of corn for the leaf fraction only (R2 = 0.86, rRMSE = 23%). These results led to the general recommendation to collect destructive area-based plant organ specific EWT measurements instead of the common practice to upscale leaf-based EWT measurements to canopy water content (CWC) by multiplication of the leaf area index (LAI). The developed and calibrated plant water retrieval (PWR) model proved to be transferable in space and time and is ready to be applied to upcoming EnMAP data and any other hyperspectral imagery. In paper II the parametric concept of spectral integral ratios (SIR) is introduced to retrieve leaf chlorophyll a and b content (Cab), leaf carotenoid content (Ccx) and leaf water content (Cw) simultaneously from imaging spectroscopy data in the wavelength range 460—1100 nm. The SIR concept is based on automatic separation of respective absorption features through local peak and intercept analysis between log-transformed reflectance and convex hulls. The approach was validated over a physiologically constrained PROSAIL simulated database, considering natural Ccx-Cab relations and green peak locations. Validation on airborne spectrometric HyMAP data achieved satisfactory results for Cab (R2 = 0.84; RMSE = 9.06 µg cm-2) and CWC (R2 = 0.70; RMSE = 0.05 cm). Retrieved Ccx values were reasonable according to Cab-Ccx-dependence plausibility analysis. Mapping of the SIR results as multiband images (3-segment SIR) allows for an intuitive visualization of dominant absorptions with respect to the three considered biochemical variables. Hence, the presented SIR algorithm allows for computationally efficient and RTM supported robust retrievals of the two most important vegetation pigments as well as of water content and is applicable on satellite imaging spectroscopy data. In paper III a hybrid workflow is presented, combining RTM with ML for inferring crop carbon content (Carea) and aboveground dry and fresh biomass (AGBdry, AGBfresh). The concept involves the establishment of a PROSAIL training database, dimensionality reduction using PCA, optimization in the sampling domain using AL against the 4-year MNI campaign dataset, and training of Gaussian process regression (GPR) ML algorithms. Internal validation of the GPR-Carea and GPR-AGB models achieved R2 of 0.80 for Carea, and R2 of 0.80 and 0.71 for AGBdry and AGBfresh, respectively. Validation with an independent dataset, comprising airborne AVIRIS NG imagery (spectrally resampled to EnMAP) and in situ measurements, successfully demonstrated mapping capabilities for both bare and green fields and generated reliable estimates over winter wheat fields at low associated model uncertainties (< 40%). Overall, the proposed carbon and biomass models demonstrate a promising path toward the inference of these crucial variables over cultivated areas from upcoming spaceborne hyperspectral acquisitions, such as from EnMAP. As conclusions, the following important findings arise regarding parametric and nonparametric hybrid methods as well as in view of the importance of in situ data collection. (1) Uncertainties within the RTM PROSAIL should always be considered. A possible reduction of these uncertainties is thereby opposed to the invertibility of the model and its intended simplicity. (2) Both physiological constraints and AL heuristics should be applied to reduce unrealistic parameter combinations in a PROSAIL calibration or training database. (3) State-of-the-art hybrid ML approaches with the ability to provide uncertainty intervals are anticipated as most promising approach for solving inference problems from hyperspectral Earth observation data due to their synergistic use of RTMs and the high flexibility, accuracy and consistency of nonlinear nonparametric methods. (4) Parametric hybrid approaches, due to their algorithmic transparency, enable deeper insights into fundamental physical limitations of optical remote sensing as compared to ML approaches. (5) Integration-based indices that make full use of available hyperspectral information may serve as physics-aware dimensionality reduced input for ML algorithms to either improve estimations or to serve as endmember for crop type discrimination when additional time series information is available. (6) The validation of quantitative model-based estimations is crucial to evaluate and improve their performance in terms of the underlying assumptions, model parameterizations, and input data. (7) In the face of soon-to-be-available EnMAP data, collection of in situ data for validation of retrieval methods should aim at high variability of measured crop types, high temporal variability over the whole growing season, as well as include area- and biomass-based destructive measurements instead of LAI-upscaled leaf measurements. Provided the perfect functionality of the payload instruments, the success of the EnMAP mission and the here presented methods depend critically on a low-noise, accurate atmospherically corrected reflectance product. High-level outputs of the retrieval methods presented in this thesis may be incorporated into agricultural decision support systems for fertilization and irrigation planning, yield estimation, or estimation of the soil carbon sequestration potential to enable a sustainable intensive agriculture in the future.Mit der am 1. April 2022 gestarteten Satellitenmission Environmental Mapping and Analysis Program (EnMAP) eröffnen sich neue Möglichkeiten für die Präzisionslandwirtschaft und das landwirtschaftliche Monitoring. Die wiederkehrende Erfassung spektrometrischer Bilder aus dem Weltraum, welche das elektromagnetische Spektrum im optischen Bereich (400—2500 nm) innerhalb von engen, schmalen Bändern zusammenhängend auflösen, liefert nie dagewesene Daten über die Interaktionen von Strahlung und biophysikalischen und biochemischen Pflanzenbestandteilen. Diese Wechselwirkungen manifestieren sich in der spektralen Reflektanz, die wichtige Informationen über den Zustand und die Gesundheit der Pflanzen enthält. Vor dem Hintergrund einer steigenden Nachfrage nach Nahrungsmitteln durch eine wachsende Weltbevölkerung können diese Informationen in landwirtschaftliche Managementsysteme einfließen, um eine notwendige Ertragsmaximierung zu unterstützen. Gleichzeitig können sie eine effiziente Optimierung der Düngung und Schädlingsbekämpfung ermöglichen, um die Umweltauswirkungen der Landwirtschaft zu minimieren. Die Ableitung biophysikalischer und biochemischer Pflanzeneigenschaften aus hyperspektralen Reflektanzdaten ist dabei immer von einem Modell abhängig. Diese Modelle werden in (1) parametrische, (2) nichtparametrische, (3) physikalisch basierte und (4) hybride Ableitungsmethoden kategorisiert. Parametrische Methoden definieren einen expliziten parametrisierten Ausdruck, der eine Reihe von Spektralkanälen oder deren Ableitungen mit einem Pflanzenmerkmal von Interesse in Beziehung setzt. Nichtparametrische Methoden umfassen lineare Techniken wie die Hauptkomponentenanalyse (PCA). Diese adressieren Kollinearitätsprobleme zwischen benachbarten Kanälen und komprimieren die gesamte Spektralinformation in dimensionsreduzierte, maximal informative Hauptkomponenten (PCs). Nichtparametrische nichtlineare Methoden, d. h. Algorithmen des maschinellen Lernens (ML), wenden nichtlineare Transformationen auf bildgebende Spektroskopiedaten an und sind daher in der Lage, nichtlineare Beziehungen innerhalb der enthaltenen spektralen Merkmale zu erfassen. Physikalisch basierte Methoden sind ein Oberbegriff für Strahlungstransfermodelle (RTM) und damit verbundene Ableitungsschemata, d. h. Invertierungsverfahren wie z. B. die Invertierung mittels Look-up-Table (LUT). Ein einfaches, leicht invertierbares und spezifisches RTM stellt das Lambert-Beer'sche Gesetz dar, das zur direkten Ableitung des Wassergehalts von Pflanzen verwendet werden kann. Das am weitesten verbreitete, allgemeine und invertierbare RTM ist das eindimensionale Bestandsmodell PROSAIL, eine Kopplung des Blattmodells Leaf Optical Properties Spectra (PROSPECT) mit dem Bestandsreflexionsmodell 4SAIL (Scattering by Arbitrarily Inclined Leaves). Bei hybriden Methoden werden von RTMs generierte, synthetische Datenbanken entweder zur Kalibrierung parametrischer Methoden oder zum Training nichtparametrischer ML-Algorithmen verwendet. Aufgrund der Äquifinalitätsproblematik bei der RTM-Invertierung, müssen potenziell unrealistische und redundante Simulationen in einer solchen Datenbank durch die Implementierung natürlicher physiologischer Beschränkungen oder durch die Anwendung von Active Learning (AL) Heuristiken entfernt werden. In dieser kumulativen Dissertation werden drei verschiedene hybride Ansätze zur Ableitung landwirtschaftlich relevanter Pflanzenmerkmale aus spektrometrischen Bilddaten vorgestellt, die anhand von drei wissenschaftlichen Publikationen demonstriert werden. In Paper I wird das Lambert-Beer'sche Gesetz angewandt, um die Dicke der optisch aktiven Wasserschicht (bzw. EWT) direkt aus dem Absorptionsmerkmal von flüssigem Wasser bei 970 nm abzuleiten. Das Modell wird mit 50.000 PROSPECT-Spektren kalibriert und anhand von In-situ-Daten validiert. Aufgrund separater Messungen des Wassergehalts von Blättern, Stängeln und Früchten während der München-Nord-Isar (MNI)-Kampagnen, zeigen die Ergebnisse, dass je nach Kulturart und -struktur, unterschiedliche Teile des Bestandes mit optischen Sensoren beobachtet werden können. Bei Winterweizen wurde die höchste Korrelation zwischen gemessenem und modelliertem Wassergehalt für Ähren und Blätter erzielt und sie erreichte Bestimmtheitsmaße (R2) von bis zu 0,72 bei einem relativen RMSE (rRMSE) von 26%, bei Mais entsprechend nur für die Blattfraktion (R2 = 0,86, rRMSE = 23%). Diese Ergebnisse führten zu der allgemeinen Empfehlung, Kompartiment-spezifische EWT-Bestandsmessungen zu erheben, anstatt der üblichen Praxis, blattbasierte EWT-Messungen durch Multiplikation mit dem Blattflächenindex (LAI) auf den Bestandswassergehalt (CWC) hochzurechnen. Das entwickelte und kalibrierte Modell zur Ableitung des Pflanzenwassergehalts (PWR) erwies sich als räumlich und zeitlich übertragbar und kann auf bald verfügbare EnMAP-Daten und andere hyperspektrale Bilddaten angewendet werden. In Paper II wird das parametrische Konzept der spektralen Integralratios (SIR) eingeführt, um den Chlorophyll a- und b-Gehalt (Cab), den Karotinoidgehalt (Ccx) und den Wassergehalt (Cw) simultan aus bildgebenden Spektroskopiedaten im Wellenlängenbereich 460-1100 nm zu ermitteln. Das SIR-Konzept basiert auf der automatischen Separierung der jeweiligen Absorptionsmerkmale durch lokale Maxima- und Schnittpunkt-Analyse zwischen log-transformierter Reflektanz und konvexen Hüllen. Der Ansatz wurde anhand einer physiologisch eingeschränkten PROSAIL-Datenbank unter Berücksichtigung natürlicher Ccx-Cab-Beziehungen und Positionen der Maxima im grünen Wellenlängenbereich validiert. Die Validierung mit flugzeuggestützten spektrometrischen HyMAP-Daten ergab zufriedenstellende Ergebnisse für Cab (R2 = 0,84; RMSE = 9,06 µg cm-2) und CWC (R2 = 0,70; RMSE = 0,05 cm). Die ermittelten Ccx-Werte wurden anhand einer Plausibilitätsanalyse entsprechend der Cab-Ccx-Abhängigkeit als sinnvoll bewertet. Die Darstellung der SIR-Ergebnisse als mehrkanalige Bilder (3 segment SIR) ermöglicht zudem eine auf die drei betrachteten biochemischen Variablen bezogene, intuitive Visualisierung der dominanten Absorptionen. Der vorgestellte SIR-Algorithmus ermöglicht somit wenig rechenintensive und RTM-gestützte robuste Ableitungen der beiden wichtigsten Pigmente sowie des Wassergehalts und kann in auf jegliche zukünftig verfügbare Hyperspektraldaten angewendet werden. In Paper III wird ein hybrider Ansatz vorgestellt, der RTM mit ML kombiniert, um den Kohlenstoffgehalt (Carea) sowie die oberirdische trockene und frische Biomasse (AGBdry, AGBfresh) abzuschätzen. Das Konzept umfasst die Erstellung einer PROSAIL-Trainingsdatenbank, die Dimensionsreduzierung mittels PCA, die Reduzierung der Stichprobenanzahl mittels AL anhand des vier Jahre umspannenden MNI-Kampagnendatensatzes und das Training von Gaussian Process Regression (GPR) ML-Algorithmen. Die interne Validierung der GPR-Carea und GPR-AGB-Modelle ergab einen R2 von 0,80 für Carea und einen R2 von 0,80 bzw. 0,71 für AGBdry und AGBfresh. Die Validierung auf einem unabhängigen Datensatz, der flugzeuggestützte AVIRIS-NG-Bilder (spektral auf EnMAP umgerechnet) und In-situ-Messungen umfasste, zeigte erfolgreich die Kartierungsfähigkeiten sowohl für offene Böden als auch für grüne Felder und führte zu zuverlässigen Schätzungen auf Winterweizenfeldern bei geringen Modellunsicherheiten (< 40%). Insgesamt zeigen die vorgeschlagenen Kohlenstoff- und Biomassemodelle einen vielversprechenden Ansatz auf, der zur Ableitung dieser wichtigen Variablen über Anbauflächen aus künftigen weltraumgestützten Hyperspektralaufnahmen wie jenen von EnMAP genutzt werden kann. Als Schlussfolgerungen ergeben sich die folgenden wichtigen Erkenntnisse in Bezug auf parametrische und nichtparametrische Hybridmethoden sowie bezogen auf die Bedeutung der In-situ-Datenerfassung. (1) Unsicherheiten innerhalb des RTM PROSAIL sollten immer berücksichtigt werden. Eine mögliche Verringerung dieser Unsicherheiten steht dabei der Invertierbarkeit des Modells und dessen beabsichtigter Einfachheit entgegen. (2) Sowohl physiologische Einschränkungen als auch AL-Heuristiken sollten angewendet werden, um unrealistische Parameterkombinationen in einer PROSAIL-Kalibrierungs- oder Trainingsdatenbank zu reduzieren. (3) Modernste ML-Ansätze mit der Fähigkeit, Unsicherheitsintervalle bereitzustellen, werden als vielversprechendster Ansatz für die Lösung von Inferenzproblemen aus hyperspektralen Erdbeobachtungsdaten aufgrund ihrer synergetischen Nutzung von RTMs und der hohen Flexibilität, Genauigkeit und Konsistenz nichtlinearer nichtparametrischer Methoden angesehen. (4) Parametrische hybride Ansätze ermöglichen aufgrund ihrer algorithmischen Transparenz im Vergleich zu ML-Ansätzen tiefere Einblicke in die grundlegenden physikalischen Grenzen der optischen Fernerkundung. (5) Integralbasierte Indizes, die die verfügbare hyperspektrale Information voll ausschöpfen, können als physikalisch-basierte dimensionsreduzierte Inputs für ML-Algorithmen dienen, um entweder Schätzungen zu verbessern oder um als Eingangsdaten die verbesserte Unterscheidung von Kulturpflanzen zu ermöglichen, sobald zusätzliche Zeitreiheninformationen verfügbar sind. (6) Die Validierung quantitativer modellbasierter Schätzungen ist von entscheidender Bedeutung für die Bewertung und Verbesserung ihrer Leistungsfähigkeit in Bezug auf die zugrunde liegenden Annahmen, Modellparametrisierungen und Eingabedaten. (7) Angesichts der bald verfügbaren EnMAP-Daten sollte die Erhebung von In-situ-Daten zur Validierung von Ableitungsmethoden auf eine hohe Variabilität der gemessenen Pflanzentypen und eine hohe zeitliche Variabilität über die gesamte Vegetationsperiode abzielen sowie flächen- und biomassebasierte destruktive Messungen anstelle von LAI-skalierten Blattmessungen umfassen. Unter der Voraussetzung, dass die Messinstrumente perfekt funktionieren, hängt der Erfolg der EnMAP-Mission und der hier vorgestellten Methoden entscheidend von einem rauscharmen, präzise atmosphärisch korrigierten Reflektanzprodukt ab. Die Ergebnisse der in dieser Arbeit vorgestellten Methoden können in landwirtschaftliche Entscheidungsunterstützungssysteme für die Dünge- oder Bewässerungsplanung, die Ertragsabschätzung oder die Schätzung des Potenzials der Kohlenstoffbindung im Boden integriert werden, um eine nachhaltige Intensivlandwirtschaft in der Zukunft zu ermöglichen