Flexible Hardware Architectures for Retinal Image Analysis

RÉSUMÉ Des millions de personnes autour du monde sont touchées par le diabète. Plusieurs complications oculaires telle que la rétinopathie diabétique sont causées par le diabète, ce qui peut conduire à une perte de vision irréversible ou même la cécité si elles ne sont pas traitées. Des examens oculaires complets et réguliers par les ophtalmologues sont nécessaires pour une détection précoce des maladies et pour permettre leur traitement. Comme solution préventive, un protocole de dépistage impliquant l'utilisation d'images numériques du fond de l'œil a été adopté. Cela permet aux ophtalmologistes de surveiller les changements sur la rétine pour détecter toute présence d'une maladie oculaire. Cette solution a permis d'obtenir des examens réguliers, même pour les populations des régions éloignées et défavorisées. Avec la grande quantité d'images rétiniennes obtenues, des techniques automatisées pour les traiter sont devenues indispensables. Les techniques automatisées de détection des maladies des yeux ont été largement abordées par la communauté scientifique. Les techniques développées ont atteint un haut niveau de maturité, ce qui a permis entre autre le déploiement de solutions en télémédecine. Dans cette thèse, nous abordons le problème du traitement de volumes élevés d'images rétiniennes dans un temps raisonnable dans un contexte de dépistage en télémédecine. Ceci est requis pour permettre l'utilisation pratique des techniques développées dans le contexte clinique. Dans cette thèse, nous nous concentrons sur deux étapes du pipeline de traitement des images rétiniennes. La première étape est l'évaluation de la qualité de l'image rétinienne. La deuxième étape est la segmentation des vaisseaux sanguins rétiniens. L’évaluation de la qualité des images rétinienne après acquisition est une tâche primordiale au bon fonctionnement de tout système de traitement automatique des images de la rétine. Le rôle de cette étape est de classifier les images acquises selon leurs qualités, et demander une nouvelle acquisition en cas d’image de mauvaise qualité. Plusieurs algorithmes pour évaluer la qualité des images rétiniennes ont été proposés dans la littérature. Cependant, même si l'accélération de cette tâche est requise en particulier pour permettre la création de systèmes mobiles de capture d'images rétiniennes, ce sujet n'a pas encore été abordé dans la littérature. Dans cette thèse, nous ciblons un algorithme qui calcule les caractéristiques des images pour permettre leur classification en mauvaise, moyenne ou bonne qualité. Nous avons identifié le calcul des caractéristiques de l'image comme une tâche répétitive qui nécessite une accélération. Nous nous sommes intéressés plus particulièrement à l’accélération de l’algorithme d’encodage à longueur de séquence (Run-Length Matrix – RLM). Nous avons proposé une première implémentation complètement logicielle mise en œuvre sous forme d’un système embarqué basé sur la technologie Zynq de Xilinx. Pour accélérer le calcul des caractéristiques, nous avons conçu un co-processeur capable de calculer les caractéristiques en parallèle implémenté sur la logique programmable du FPGA Zynq. Nous avons obtenu une accélération de 30,1 × pour la tâche de calcul des caractéristiques de l’algorithme RLM par rapport à son implémentation logicielle sur la plateforme Zynq. La segmentation des vaisseaux sanguins rétiniens est une tâche clé dans le pipeline du traitement des images de la rétine. Les vaisseaux sanguins et leurs caractéristiques sont de bons indicateurs de la santé de la rétine. En outre, leur segmentation peut également aider à segmenter les lésions rouges, indicatrices de la rétinopathie diabétique. Plusieurs techniques de segmentation des vaisseaux sanguins rétiniens ont été proposées dans la littérature. Des architectures matérielles ont également été proposées pour accélérer certaines de ces techniques. Les architectures existantes manquent de performances et de flexibilité de programmation, notamment pour les images de haute résolution. Dans cette thèse, nous nous sommes intéressés à deux techniques de segmentation du réseau vasculaire rétinien, la technique du filtrage adapté et la technique des opérateurs de ligne. La technique de filtrage adapté a été ciblée principalement en raison de sa popularité. Pour cette technique, nous avons proposé deux architectures différentes, une architecture matérielle personnalisée mise en œuvre sur FPGA et une architecture basée sur un ASIP. L'architecture matérielle personnalisée a été optimisée en termes de surface et de débit de traitement pour obtenir des performances supérieures par rapport aux implémentations existantes dans la littérature. Cette implémentation est plus efficace que toutes les implémentations existantes en termes de débit. Pour l'architecture basée sur un processeur à jeu d’instructions spécialisé (Application-Specific Instruction-set Processor – ASIP), nous avons identifié deux goulets d'étranglement liés à l'accès aux données et à la complexité des calculs de l'algorithme. Nous avons conçu des instructions spécifiques ajoutées au chemin de données du processeur. L'ASIP a été rendu 7.7 × plus rapide par rapport à son architecture de base. La deuxième technique pour la segmentation des vaisseaux sanguins est l'algorithme détecteur de ligne multi-échelle (Multi-Scale Ligne Detector – MSLD). L'algorithme MSLD est choisi en raison de ses performances et de son potentiel à détecter les petits vaisseaux sanguins. Cependant, l'algorithme fonctionne en multi-échelle, ce qui rend l’algorithme gourmand en mémoire. Pour résoudre ce problème et permettre l'accélération de son exécution, nous avons proposé un algorithme efficace en terme de mémoire, conçu et implémenté sur FPGA. L'architecture proposée a réduit de façon drastique les exigences de l’algorithme en terme de mémoire en réutilisant les calculs et la co-conception logicielle/matérielle. Les deux architectures matérielles proposées pour la segmentation du réseau vasculaire rétinien ont été rendues flexibles pour pouvoir traiter des images de basse et de haute résolution. Ceci a été réalisé par le développement d'un compilateur spécifique capable de générer une description HDL de bas niveau de l'algorithme à partir d'un ensemble de paramètres. Le compilateur nous a permis d’optimiser les performances et le temps de développement. Dans cette thèse, nous avons introduit deux architectures qui sont, au meilleur de nos connaissances, les seules capables de traiter des images à la fois de basse et de haute résolution.----------ABSTRACT Millions of people all around the world are affected by diabetes. Several ocular complications such as diabetic retinopathy are caused by diabetes, which can lead to irreversible vision loss or even blindness if not treated. Regular comprehensive eye exams by eye doctors are required to detect the diseases at earlier stages and permit their treatment. As a preventing solution, a screening protocol involving the use of digital fundus images was adopted. This allows eye doctors to monitor changes in the retina to detect any presence of eye disease. This solution made regular examinations widely available, even to populations in remote and underserved areas. With the resulting large amount of retinal images, automated techniques to process them are required. Automated eye detection techniques are largely addressed by the research community, and now they reached a high level of maturity, which allows the deployment of telemedicine solutions. In this thesis, we are addressing the problem of processing a high volume of retinal images in a reasonable time. This is mandatory to allow the practical use of the developed techniques in a clinical context. In this thesis, we focus on two steps of the retinal image pipeline. The first step is the retinal image quality assessment. The second step is the retinal blood vessel segmentation. The evaluation of the quality of the retinal images after acquisition is a primary task for the proper functioning of any automated retinal image processing system. The role of this step is to classify the acquired images according to their quality, which will allow an automated system to request a new acquisition in case of poor quality image. Several algorithms to evaluate the quality of retinal images were proposed in the literature. However, even if the acceleration of this task is required, especially to allow the creation of mobile systems for capturing retinal images, this task has not yet been addressed in the literature. In this thesis, we target an algorithm that computes image features to allow their classification to bad, medium or good quality. We identified the computation of image features as a repetitive task that necessitates acceleration. We were particularly interested in accelerating the Run-Length Matrix (RLM) algorithm. We proposed a first fully software implementation in the form of an embedded system based on Xilinx's Zynq technology. To accelerate the features computation, we designed a co-processor able to compute the features in parallel, implemented on the programmable logic of the Zynq FPGA. We achieved an acceleration of 30.1× over its software implementation for the features computation part of the RLM algorithm. Retinal blood vessel segmentation is a key task in the pipeline of retinal image processing. Blood vessels and their characteristics are good indicators of retina health. In addition, their segmentation can also help to segment the red lesions, indicators of diabetic retinopathy. Several techniques have been proposed in the literature to segment retinal blood vessels. Hardware architectures have also been proposed to accelerate blood vessel segmentation. The existing architectures lack in terms of performance and programming flexibility, especially for high resolution images. In this thesis, we targeted two techniques, matched filtering and line operators. The matched filtering technique was targeted mainly because of its popularity. For this technique, we proposed two different architectures, a custom hardware architecture implemented on FPGA, and an Application Specific Instruction-set Processor (ASIP) based architecture. The custom hardware architecture area and timing were optimized to achieve higher performances in comparison to existing implementations. Our custom hardware implementation outperforms all existing implementations in terms of throughput. For the ASIP based architecture, we identified two bottlenecks related to data access and computation intensity of the algorithm. We designed two specific instructions added to the processor datapath. The ASIP was made 7.7× more efficient in terms of execution time compared to its basic architecture. The second technique for blood vessel segmentation is the Multi-Scale Line Detector (MSLD) algorithm. The MSLD algorithm is selected because of its performance and its potential to detect small blood vessels. However, the algorithm works at multiple scales which makes it memory intensive. To solve this problem and allow the acceleration of its execution, we proposed a memory-efficient algorithm designed and implemented on FPGA. The proposed architecture reduces drastically the memory requirements of the algorithm by reusing the computations and SW/HW co-design. The two hardware architectures proposed for retinal blood vessel segmentation were made flexible to be able to process low and high resolution images. This was achieved by the development of a specific compiler able to generate low-level HDL descriptions of the algorithm from a set of the algorithm parameters. The compiler enabled us to optimize performance and development time. In this thesis, we introduce two novel architectures which are, to the best of our knowledge, the only ones able to process both low and high resolution images

Digital ocular fundus imaging: a review

Ocular fundus imaging plays a key role in monitoring the health status of the human eye. Currently, a large number of imaging modalities allow the assessment and/or quantification of ocular changes from a healthy status. This review focuses on the main digital fundus imaging modality, color fundus photography, with a brief overview of complementary techniques, such as fluorescein angiography. While focusing on two-dimensional color fundus photography, the authors address the evolution from nondigital to digital imaging and its impact on diagnosis. They also compare several studies performed along the transitional path of this technology. Retinal image processing and analysis, automated disease detection and identification of the stage of diabetic retinopathy (DR) are addressed as well. The authors emphasize the problems of image segmentation, focusing on the major landmark structures of the ocular fundus: the vascular network, optic disk and the fovea. Several proposed approaches for the automatic detection of signs of disease onset and progression, such as microaneurysms, are surveyed. A thorough comparison is conducted among different studies with regard to the number of eyes/subjects, imaging modality, fundus camera used, field of view and image resolution to identify the large variation in characteristics from one study to another. Similarly, the main features of the proposed classifications and algorithms for the automatic detection of DR are compared, thereby addressing computer-aided diagnosis and computer-aided detection for use in screening programs.Fundação para a Ciência e TecnologiaFEDErPrograma COMPET

NON-INVASIVE IMAGE ENHANCEMENT OF COLOUR RETINAL FUNDUS IMAGES FOR A COMPUTERISED DIABETIC RETINOPATHY MONITORING AND GRADING SYSTEM

Diabetic Retinopathy (DR) is a sight threatening complication due to diabetes mellitus affecting the retina. The pathologies of DR can be monitored by analysing colour fundus images. However, the low and varied contrast between retinal vessels and the background in colour fundus images remains an impediment to visual analysis in particular in analysing tiny retinal vessels and capillary networks. To circumvent this problem, fundus fluorescein angiography (FF A) that improves the image contrast is used. Unfortunately, it is an invasive procedure (injection of contrast dyes) that leads to other physiological problems and in the worst case may cause death. The objective of this research is to develop a non-invasive digital Image enhancement scheme that can overcome the problem of the varied and low contrast colour fundus images in order that the contrast produced is comparable to the invasive fluorescein method, and without introducing noise or artefacts. The developed image enhancement algorithm (called RETICA) is incorporated into a newly developed computerised DR system (called RETINO) that is capable to monitor and grade DR severity using colour fundus images. RETINO grades DR severity into five stages, namely No DR, Mild Non Proliferative DR (NPDR), Moderate NPDR, Severe NPDR and Proliferative DR (PDR) by enhancing the quality of digital colour fundus image using RETICA in the macular region and analysing the enlargement of the foveal avascular zone (F AZ), a region devoid of retinal vessels in the macular region. The importance of this research is to improve image quality in order to increase the accuracy, sensitivity and specificity of DR diagnosis, and to enable DR grading through either direct observation or computer assisted diagnosis system

NON-INVASIVE IMAGE ENHANCEMENT OF COLOUR RETINAL FUNDUS IMAGES FOR A COMPUTERISED DIABETIC RETINOPATHY MONITORING AND GRADING SYSTEM

Diabetic Retinopathy (DR) is a sight threatening complication due to diabetes mellitus affecting the retina. The pathologies of DR can be monitored by analysing colour fundus images. However, the low and varied contrast between retinal vessels and the background in colour fundus images remains an impediment to visual analysis in particular in analysing tiny retinal vessels and capillary networks. To circumvent this problem, fundus fluorescein angiography (FF A) that improves the image contrast is used. Unfortunately, it is an invasive procedure (injection of contrast dyes) that leads to other physiological problems and in the worst case may cause death. The objective of this research is to develop a non-invasive digital Image enhancement scheme that can overcome the problem of the varied and low contrast colour fundus images in order that the contrast produced is comparable to the invasive fluorescein method, and without introducing noise or artefacts. The developed image enhancement algorithm (called RETICA) is incorporated into a newly developed computerised DR system (called RETINO) that is capable to monitor and grade DR severity using colour fundus images. RETINO grades DR severity into five stages, namely No DR, Mild Non Proliferative DR (NPDR), Moderate NPDR, Severe NPDR and Proliferative DR (PDR) by enhancing the quality of digital colour fundus image using RETICA in the macular region and analysing the enlargement of the foveal avascular zone (F AZ), a region devoid of retinal vessels in the macular region. The importance of this research is to improve image quality in order to increase the accuracy, sensitivity and specificity of DR diagnosis, and to enable DR grading through either direct observation or computer assisted diagnosis system

Performance analysis of massively parallel embedded hardware architectures for retinal image processing

This paper examines the implementation of a retinal vessel tree extraction technique on different hardware platforms and architectures. Retinal vessel tree extraction is a representative application of those found in the domain of medical image processing. The low signal-to-noise ratio of the images leads to a large amount of low-level tasks in order to meet the accuracy requirements. In some applications, this might compromise computing speed. This paper is focused on the assessment of the performance of a retinal vessel tree extraction method on different hardware platforms. In particular, the retinal vessel tree extraction method is mapped onto a massively parallel SIMD (MP-SIMD) chip, a massively parallel processor array (MPPA) and onto an field-programmable gate arrays (FPGA)This work is funded by Xunta de Galicia under the projects 10PXIB206168PR and 10PXIB206037PR and the program Maria BarbeitoS

Image Processing and Analysis for Preclinical and Clinical Applications

Radiomics is one of the most successful branches of research in the field of image processing and analysis, as it provides valuable quantitative information for the personalized medicine. It has the potential to discover features of the disease that cannot be appreciated with the naked eye in both preclinical and clinical studies. In general, all quantitative approaches based on biomedical images, such as positron emission tomography (PET), computed tomography (CT) and magnetic resonance imaging (MRI), have a positive clinical impact in the detection of biological processes and diseases as well as in predicting response to treatment. This Special Issue, “Image Processing and Analysis for Preclinical and Clinical Applications”, addresses some gaps in this field to improve the quality of research in the clinical and preclinical environment. It consists of fourteen peer-reviewed papers covering a range of topics and applications related to biomedical image processing and analysis

Handbook of Vascular Biometrics

This open access handbook provides the first comprehensive overview of biometrics exploiting the shape of human blood vessels for biometric recognition, i.e. vascular biometrics, including finger vein recognition, hand/palm vein recognition, retina recognition, and sclera recognition. After an introductory chapter summarizing the state of the art in and availability of commercial systems and open datasets/open source software, individual chapters focus on specific aspects of one of the biometric modalities, including questions of usability, security, and privacy. The book features contributions from both academia and major industrial manufacturers

U-net and its variants for medical image segmentation: A review of theory and applications

U-net is an image segmentation technique developed primarily for image segmentation tasks. These traits provide U-net with a high utility within the medical imaging community and have resulted in extensive adoption of U-net as the primary tool for segmentation tasks in medical imaging. The success of U-net is evident in its widespread use in nearly all major image modalities, from CT scans and MRI to Xrays and microscopy. Furthermore, while U-net is largely a segmentation tool, there have been instances of the use of U-net in other applications. Given that U-net’s potential is still increasing, this narrative literature review examines the numerous developments and breakthroughs in the U-net architecture and provides observations on recent trends. We also discuss the many innovations that have advanced in deep learning and discuss how these tools facilitate U-net. In addition, we review the different image modalities and application areas that have been enhanced by U-net