Flower colours through the lens: Quantitative measurement with visible and ultraviolet digital photography

Background: The study of the signal-receiver relationship between flowering plants and pollinators requires a capacity to accurately map both the spectral and spatial components of a signal in relation to the perceptual abilities of potential pollinators. Spectrophotometers can typically recover high resolution spectral data, but the spatial component is difficult to record simultaneously. A technique allowing for an accurate measurement of the spatial component in addition to the spectral factor of the signal is highly desirable

Camera characterization for improving color archaeological documentation

[EN] Determining the correct color is essential for proper cultural heritage documentation and cataloging. However, the methodology used in most cases limits the results since it is based either on perceptual procedures or on the application of color profiles in digital processing software. The objective of this study is to establish a rigorous procedure, from the colorimetric point of view, for the characterization of cameras, following different polynomial models. Once the camera is characterized, users obtain output images in the sRGB space that is independent of the sensor of the camera. In this article we report on pyColorimetry software that was developed and tested taking into account the recommendations of the Commission Internationale de l’Eclairage (CIE). This software allows users to control the entire digital image processing and the colorimetric data workflow, including the rigorous processing of raw data. We applied the methodology on a picture targeting Levantine rock art motifs in Remigia Cave (Spain) that is considered part of a UNESCO World Heritage Site. Three polynomial models were tested for the transformation between color spaces. The outcomes obtained were satisfactory and promising, especially with RAW files. The best results were obtained with a second-order polynomial model, achieving residuals below three CIELAB units. We highlight several factors that must be taken into account, such as the geometry of the shot and the light conditions, which are determining factors for the correct characterization of a digital camera.The authors gratefully acknowledge the support from the Spanish Ministerio de Economia y Competitividad to the project HAR2014-59873-R. The authors would like also to acknowledge the comments from the colleagues at the Photogrammetry & Laser Scanning Research Group (GIFLE) and the fruitful discussions provided by Archaeologist Dr. Esther Lopez-Montalvo.Molada Tebar, A.; Lerma García, JL.; Marqués Mateu, Á. (2017). Camera characterization for improving color archaeological documentation. Color Research and Application. 43(1):47-57. https://doi.org/10.1002/col.22152S475743

Algorithms for the enhancement of dynamic range and colour constancy of digital images & video

One of the main objectives in digital imaging is to mimic the capabilities of the human eye, and perhaps, go beyond in certain aspects. However, the human visual system is so versatile, complex, and only partially understood that no up-to-date imaging technology has been able to accurately reproduce the capabilities of the it. The extraordinary capabilities of the human eye have become a crucial shortcoming in digital imaging, since digital photography, video recording, and computer vision applications have continued to demand more realistic and accurate imaging reproduction and analytic capabilities. Over decades, researchers have tried to solve the colour constancy problem, as well as extending the dynamic range of digital imaging devices by proposing a number of algorithms and instrumentation approaches. Nevertheless, no unique solution has been identified; this is partially due to the wide range of computer vision applications that require colour constancy and high dynamic range imaging, and the complexity of the human visual system to achieve effective colour constancy and dynamic range capabilities. The aim of the research presented in this thesis is to enhance the overall image quality within an image signal processor of digital cameras by achieving colour constancy and extending dynamic range capabilities. This is achieved by developing a set of advanced image-processing algorithms that are robust to a number of practical challenges and feasible to be implemented within an image signal processor used in consumer electronics imaging devises. The experiments conducted in this research show that the proposed algorithms supersede state-of-the-art methods in the fields of dynamic range and colour constancy. Moreover, this unique set of image processing algorithms show that if they are used within an image signal processor, they enable digital camera devices to mimic the human visual system s dynamic range and colour constancy capabilities; the ultimate goal of any state-of-the-art technique, or commercial imaging device

Hyperspectral image analysis techniques for the detection and classification of the early onset of plant disease and stress

This review explores how imaging techniques are being developed with a focus on deployment for crop monitoring methods. Imaging applications are discussed in relation to both field and glasshouse-based plants, and techniques are sectioned into ‘healthy and diseased plant classification’ with an emphasis on classification accuracy, early detection of stress, and disease severity. A central focus of the review is the use of hyperspectral imaging and how this is being utilised to find additional information about plant health, and the ability to predict onset of disease. A summary of techniques used to detect biotic and abiotic stress in plants is presented, including the level of accuracy associated with each method

Laboratory Characterisation of a Commercial RGB CMOS Camera for Measuring Night Sky Brightness

The use of RGB cameras in photometric applications has grown over the last few decades in many fields such as industrial applications, light engineering and the analysis of the quality of the night sky. In this last field, they are often used in conjunction with a Sky Quality Meter (SQM), an instrument used for the measurement of night sky brightness (NSB), mainly when there is a significant amount of artificial light at night (ALAN). The performances of these two instruments are compared here. A simple source composed of nine narrowband LEDs in an integrating sphere was used to excite the two instruments and therefore measure the spectral responsivity of the SQM and of the three channels of the camera. The estimated uncertainties regarding spectral responsivity were less than 10%. A synthetic instrument approximating the SQM's responsivity can be created using a combination of the R, G and B channels. The outputs of the two instruments were compared by measuring the spectral radiance of the night sky. An evaluation of the spectral mismatch between the two instruments completed the analysis of their spectral sensitivity. Finally, the measurements of real SQMs in four sites experiencing different levels of light pollution were compared with the values obtained by processing the recorded RGB images. Overall, the analysis shows that the two instruments have significantly different levels of spectral responsivity, and the alignment of their outputs requires the use of a correction which depends on the spectral distribution of the light coming from the sky. A synthetic SQM will always underestimate real SQM measures; an average correction factor was evaluated considering nine sky spectra under low and medium levels of light pollution; this was determined to be 1.11 and, on average, compensated for the gap. A linear correction was also supposed based on the correlation between the NSB levels measured by the two instruments; the mean squared error after the correction was 0.03 mag arcsec-2

Characterisation of a multispectral digital camera System for quantitatively comparing complex animal Patterns in natural environments.

Animal coloration can be described by complex colour patterns including elements of varying size, shape and spectral profile which commonly reflect energy outside the spectral range visible for humans. Whilst spectrometry is currently employed for the quantitative study of animal coloration, it is limited on its ability to describe the spatial characteristics of spectral differences in patterns. Digital photography has recently been used as a tool for measuring spatial and spectral properties of patterns based on quantitative analysis of linear camera responses recovered after characterising the device. However current applications of digital imaging for studying animal coloration are limited to image recording within a laboratory environment considering controlled lighting conditions. Here a refined methodology for camera characterisation is developed permitting the recording of images under different illumination conditions typical of natural environments. The characterised camera system thus allows recording images from reflected ultraviolet and visible radiation resulting in a multispectral digital camera system. Furthermore a standardised imaging processing workflow was developed based on specific characteristics of the camera thus making possible an objective comparison from images. An application of the characterised camera system is exemplified in the study of animal colour patterns adapted for camouflage using as a model two Australian, endemic lizard species. The interaction between the spectral and spatial properties of the respective lizards produces complex patterns than cannot be interpreted by spectrophotometry alone. Data obtained from analysis of images recorded with the characterised camera system in the visible and near-ultraviolet region of the spectrum reveal significative differences between sex and species and a possible interaction between sex and species, suggesting microhabitat specialisation to different backgrounds

Quantitative full-colour transmitted light microscopy and dyes for concentration mapping and measurement of diffusion coefficients in microfluidic architectures

International audienceA simple and versatile methodology has been developed for the simultaneous measurement of multiple concentration profiles of colourants in transparent microfluidic systems, using a conventional transmitted light microscope, a digital colour (RGB) camera and numerical image processing combined with multicomponent analysis. Rigorous application of the Beer-Lambert law would require monochromatic probe conditions, but in spite of the broad spectral bandwidths of the three colour channels of the camera, a linear relation between the measured optical density and dye concentration is established under certain conditions. An optimised collection of dye solutions for the quantitative optical microscopic characterisation of microfluidic devices is proposed. Using the methodology for optical concentration measurement we then implement and validate a simplified and robust method for the microfluidic measurement of diffusion coefficients using an H-filter architecture. It consists of measuring the ratio of the concentrations of the two output channels of the H-filter. It enables facile determination of the diffusion coefficient, even for non-fluorescent molecules and nanoparticles, and is compatible with non-optical detection of the analyte