Patch-based Convolutional Neural Network for Whole Slide Tissue Image Classification

Convolutional Neural Networks (CNN) are state-of-the-art models for many image classification tasks. However, to recognize cancer subtypes automatically, training a CNN on gigapixel resolution Whole Slide Tissue Images (WSI) is currently computationally impossible. The differentiation of cancer subtypes is based on cellular-level visual features observed on image patch scale. Therefore, we argue that in this situation, training a patch-level classifier on image patches will perform better than or similar to an image-level classifier. The challenge becomes how to intelligently combine patch-level classification results and model the fact that not all patches will be discriminative. We propose to train a decision fusion model to aggregate patch-level predictions given by patch-level CNNs, which to the best of our knowledge has not been shown before. Furthermore, we formulate a novel Expectation-Maximization (EM) based method that automatically locates discriminative patches robustly by utilizing the spatial relationships of patches. We apply our method to the classification of glioma and non-small-cell lung carcinoma cases into subtypes. The classification accuracy of our method is similar to the inter-observer agreement between pathologists. Although it is impossible to train CNNs on WSIs, we experimentally demonstrate using a comparable non-cancer dataset of smaller images that a patch-based CNN can outperform an image-based CNN

Picasso, Matisse, or a Fake? Automated Analysis of Drawings at the Stroke Level for Attribution and Authentication

This paper proposes a computational approach for analysis of strokes in line drawings by artists. We aim at developing an AI methodology that facilitates attribution of drawings of unknown authors in a way that is not easy to be deceived by forged art. The methodology used is based on quantifying the characteristics of individual strokes in drawings. We propose a novel algorithm for segmenting individual strokes. We designed and compared different hand-crafted and learned features for the task of quantifying stroke characteristics. We also propose and compare different classification methods at the drawing level. We experimented with a dataset of 300 digitized drawings with over 80 thousands strokes. The collection mainly consisted of drawings of Pablo Picasso, Henry Matisse, and Egon Schiele, besides a small number of representative works of other artists. The experiments shows that the proposed methodology can classify individual strokes with accuracy 70%-90%, and aggregate over drawings with accuracy above 80%, while being robust to be deceived by fakes (with accuracy 100% for detecting fakes in most settings)

Automated segmentation of tissue images for computerized IHC analysis

This paper presents two automated methods for the segmentation ofimmunohistochemical tissue images that overcome the limitations of themanual approach aswell as of the existing computerized techniques. The first independent method, based on unsupervised color clustering, recognizes automatically the target cancerous areas in the specimen and disregards the stroma; the second method, based on colors separation and morphological processing, exploits automated segmentation of the nuclear membranes of the cancerous cells. Extensive experimental results on real tissue images demonstrate the accuracy of our techniques compared to manual segmentations; additional experiments show that our techniques are more effective in immunohistochemical images than popular approaches based on supervised learning or active contours. The proposed procedure can be exploited for any applications that require tissues and cells exploration and to perform reliable and standardized measures of the activity of specific proteins involved in multi-factorial genetic pathologie

Object-Based Greenhouse Classification from GeoEye-1 and WorldView-2 Stereo Imagery

Remote sensing technologies have been commonly used to perform greenhouse detection and mapping. In this research, stereo pairs acquired by very high-resolution optical satellites GeoEye-1 (GE1) and WorldView-2 (WV2) have been utilized to carry out the land cover classification of an agricultural area through an object-based image analysis approach, paying special attention to greenhouses extraction. The main novelty of this work lies in the joint use of single-source stereo-photogrammetrically derived heights and multispectral information from both panchromatic and pan-sharpened orthoimages. The main features tested in this research can be grouped into different categories, such as basic spectral information, elevation data (normalized digital surface model; nDSM), band indexes and ratios, texture and shape geometry. Furthermore, spectral information was based on both single orthoimages and multiangle orthoimages. The overall accuracy attained by applying nearest neighbor and support vector machine classifiers to the four multispectral bands of GE1 were very similar to those computed from WV2, for either four or eight multispectral bands. Height data, in the form of nDSM, were the most important feature for greenhouse classification. The best overall accuracy values were close to 90%, and they were not improved by using multiangle orthoimages