23,244 research outputs found
Nanoclustering as a dominant feature of plasma membrane organization
Early studies have revealed that some mammalian plasma membrane proteins exist in small nanoclusters. The advent of super-resolution microscopy has corroborated and extended this picture, and led to the suggestion that many, if not most, membrane proteins are clustered at the plasma membrane at nanoscale lengths. In this Commentary, we present selected examples of glycosylphosphatidyl-anchored proteins, Ras family members and several immune receptors that provide evidence for nanoclustering. We advocate the view that nanoclustering is an important part of the hierarchical organization of proteins in the plasma membrane. According to this emerging picture, nanoclusters can be organized on the mesoscale to form microdomains that are capable of supporting cell adhesion, pathogen binding and immune cell-cell recognition amongst other functions. Yet, a number of outstanding issues concerning nanoclusters remain open, including the details of their molecular composition, biogenesis, size, stability, function and regulation. Notions about these details are put forth and suggestions are made about nanocluster function and why this general feature of protein nanoclustering appears to be so prevalent.Postprint (published version
Interactions of Poly(amidoamine) Dendrimers with Human Serum Albumin: Binding Constants and Mechanisms
The interactions of nanomaterials with plasma proteins have a significant impact on their in vivo transport and fate in biological fluids. This article discusses the binding of human serum albumin (HSA) to poly(amidoamine) [PAMAM] dendrimers. We use protein-coated silica particles to measure the HSA binding constants (K_b) of a homologous series of 19 PAMAM dendrimers in aqueous solutions at physiological pH (7.4) as a function of dendrimer generation, terminal group, and core chemistry. To gain insight into the mechanisms of HSA binding to PAMAM dendrimers, we combined ^1H NMR, saturation transfer difference (STD) NMR, and NMR diffusion ordered spectroscopy (DOSY) of dendrimer−HSA complexes with atomistic molecular dynamics (MD) simulations of dendrimer conformation in aqueous solutions. The binding measurements show that the HSA binding constants (K_b) of PAMAM dendrimers depend on dendrimer size and terminal group chemistry. The NMR ^1H and DOSY experiments indicate that the interactions between HSA and PAMAM dendrimers are relatively weak. The ^1H NMR STD experiments and MD simulations suggest that the inner shell protons of the dendrimers groups interact more strongly with HSA proteins. These interactions, which are consistently observed for different dendrimer generations (G0-NH_2vs G4-NH_2) and terminal groups (G4-NH_2vs G4-OH with amidoethanol groups), suggest that PAMAM dendrimers adopt backfolded configurations as they form weak complexes with HSA proteins in aqueous solutions at physiological pH (7.4)
A Framework for Interactive Teaching of Virtual Borders to Mobile Robots
The increasing number of robots in home environments leads to an emerging
coexistence between humans and robots. Robots undertake common tasks and
support the residents in their everyday life. People appreciate the presence of
robots in their environment as long as they keep the control over them. One
important aspect is the control of a robot's workspace. Therefore, we introduce
virtual borders to precisely and flexibly define the workspace of mobile
robots. First, we propose a novel framework that allows a person to
interactively restrict a mobile robot's workspace. To show the validity of this
framework, a concrete implementation based on visual markers is implemented.
Afterwards, the mobile robot is capable of performing its tasks while
respecting the new virtual borders. The approach is accurate, flexible and less
time consuming than explicit robot programming. Hence, even non-experts are
able to teach virtual borders to their robots which is especially interesting
in domains like vacuuming or service robots in home environments.Comment: 7 pages, 6 figure
This Far, No Further: Introducing Virtual Borders to Mobile Robots Using a Laser Pointer
We address the problem of controlling the workspace of a 3-DoF mobile robot.
In a human-robot shared space, robots should navigate in a human-acceptable way
according to the users' demands. For this purpose, we employ virtual borders,
that are non-physical borders, to allow a user the restriction of the robot's
workspace. To this end, we propose an interaction method based on a laser
pointer to intuitively define virtual borders. This interaction method uses a
previously developed framework based on robot guidance to change the robot's
navigational behavior. Furthermore, we extend this framework to increase the
flexibility by considering different types of virtual borders, i.e. polygons
and curves separating an area. We evaluated our method with 15 non-expert users
concerning correctness, accuracy and teaching time. The experimental results
revealed a high accuracy and linear teaching time with respect to the border
length while correctly incorporating the borders into the robot's navigational
map. Finally, our user study showed that non-expert users can employ our
interaction method.Comment: Accepted at 2019 Third IEEE International Conference on Robotic
Computing (IRC), supplementary video: https://youtu.be/lKsGp8xtyI
Systems microscopy approaches to understand cancer cell migration and metastasis
Cell migration is essential in a number of processes, including wound healing, angiogenesis and cancer metastasis. Especially, invasion of cancer cells in the surrounding tissue is a crucial step that requires increased cell motility. Cell migration is a well-orchestrated process that involves the continuous formation and disassembly of matrix adhesions. Those structural anchor points interact with the extra-cellular matrix and also participate in adhesion-dependent signalling. Although these processes are essential for cancer metastasis, little is known about the molecular mechanisms that regulate adhesion dynamics during tumour cell migration. In this review, we provide an overview of recent advanced imaging strategies together with quantitative image analysis that can be implemented to understand the dynamics of matrix adhesions and its molecular components in relation to tumour cell migration. This dynamic cell imaging together with multiparametric image analysis will help in understanding the molecular mechanisms that define cancer cell migration
Versatile Graphene-Based Platform for Robust Nanobiohybrid Interfaces
Technologically useful and robust graphene-based interfaces for devices
require the introduction of highly selective, stable, and covalently bonded
functionalities on the graphene surface, whilst essentially retaining the
electronic properties of the pristine layer. This work demonstrates that highly
controlled, ultrahigh vacuum covalent chemical functionalization of graphene
sheets with a thiol-terminated molecule provides a robust and tunable platform
for the development of hybrid nanostructures in different environments. We
employ this facile strategy to covalently couple two representative systems of
broad interest: metal nanoparticles, via S-metal bonds, and thiol-modified DNA
aptamers, via disulfide bridges. Both systems, which have been characterized by
a multi-technique approach, remain firmly anchored to the graphene surface even
after several washing cycles. Atomic force microscopy images demonstrate that
the conjugated aptamer retains the functionality required to recognize a target
protein. This methodology opens a new route to the integration of high-quality
graphene layers into diverse technological platforms, including plasmonics,
optoelectronics, or biosensing. With respect to the latter, the viability of a
thiol-functionalized chemical vapor deposition graphene-based solution-gated
field-effect transistor array was assessed
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