1,881 research outputs found
Holographic particle localization under multiple scattering
We introduce a novel framework that incorporates multiple scattering for
large-scale 3D particle-localization using single-shot in-line holography.
Traditional holographic techniques rely on single-scattering models which
become inaccurate under high particle-density. We demonstrate that by
exploiting multiple-scattering, localization is significantly improved. Both
forward and back-scattering are computed by our method under a tractable
recursive framework, in which each recursion estimates the next higher-order
field within the volume. The inverse scattering is presented as a nonlinear
optimization that promotes sparsity, and can be implemented efficiently. We
experimentally reconstruct 100 million object voxels from a single 1-megapixel
hologram. Our work promises utilization of multiple scattering for versatile
large-scale applications
Registration of phase contrast images in propagation-based X-ray phase tomography
International audienceX-ray phase tomography aims at reconstructing the 3D electron density distribution of an object. It offers enhanced sensitivity compared to attenuation-based X-ray absorption tomography. In propagation-based methods, phase contrast is achieved by letting the beam propagate after interaction with the object. The phase shift is then retrieved at each projection angle, and subsequently used in tomographic reconstruction to obtain the refractive index decrement distribution, which is proportional to the electron density. Accurate phase retrieval is achieved by combining images at different propagation distances. For reconstructions of good quality, the phase-contrast images recorded at different distances need to be accurately aligned. In this work, we characterise the artefacts related to misalignment of the phase-contrast images, and investigate the use of different registration algorithms for aligning in-line phase-contrast images. The characterisation of artefacts is done by a simulation study and comparison with experimental data. Loss in resolution due to vibrations is found to be comparable to attenuation-based computed tomography. Further, it is shown that registration of phase-contrast images is nontrivial due to the difference in contrast between the different images, and the often periodical artefacts present in the phase-contrast images if multilayer X-ray optics are used. To address this, we compared two registration algorithms for aligning phase-contrast images acquired by magnified X-ray nanotomography: one based on cross-correlation and one based on mutual information. We found that the mutual information-based registration algorithm was more robust than a correlation-based method
High-resolution ab initio three-dimensional X-ray diffraction microscopy
Coherent X-ray diffraction microscopy is a method of imaging non-periodic
isolated objects at resolutions only limited, in principle, by the largest
scattering angles recorded. We demonstrate X-ray diffraction imaging with high
resolution in all three dimensions, as determined by a quantitative analysis of
the reconstructed volume images. These images are retrieved from the 3D
diffraction data using no a priori knowledge about the shape or composition of
the object, which has never before been demonstrated on a non-periodic object.
We also construct 2D images of thick objects with infinite depth of focus
(without loss of transverse spatial resolution). These methods can be used to
image biological and materials science samples at high resolution using X-ray
undulator radiation, and establishes the techniques to be used in
atomic-resolution ultrafast imaging at X-ray free-electron laser sources.Comment: 22 pages, 11 figures, submitte
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Lensfree computational microscopy tools for cell and tissue imaging at the point-of-care and in low-resource settings.
The recent revolution in digital technologies and information processing methods present important opportunities to transform the way optical imaging is performed, particularly toward improving the throughput of microscopes while at the same time reducing their relative cost and complexity. Lensfree computational microscopy is rapidly emerging toward this end, and by discarding lenses and other bulky optical components of conventional imaging systems, and relying on digital computation instead, it can achieve both reflection and transmission mode microscopy over a large field-of-view within compact, cost-effective and mechanically robust architectures. Such high throughput and miniaturized imaging devices can provide a complementary toolset for telemedicine applications and point-of-care diagnostics by facilitating complex and critical tasks such as cytometry and microscopic analysis of e.g., blood smears, Pap tests and tissue samples. In this article, the basics of these lensfree microscopy modalities will be reviewed, and their clinically relevant applications will be discussed
Signal processing based method for solving inverse scattering problems
The problem of reconstructing an image of the permittivity distribution inside a penetrable and strongly scattering object from a finite number of noisy scattered field measurements has always been very challenging because it is ill-posed in nature. Several techniques have been developed which are either computationally very expensive or typically require the object to be weakly scattering. I have developed here a non-linear signal processing method, which will recover images for both strong scatterers and weak scatterers. This nonlinear or cepstral filtering method requires that the scattered field data is first preprocessed to generate a minimum phase function in the object domain. In 2-D or higher dimensional problems, I describe the conditions for minimum phase and demonstrate how an artificial reference wave can be numerically combined with measured complex scattering data in order to enforce this condition, by satisfying Rouche‘s theorem. In the cepstral domain one can filter the frequencies associated with an object from those of the scattered field. After filtering, the next step is to inverse Fourier transform these data and exponentiate to recover the image of the object under test. In addition I also investigate the scattered field sampling requirements for the inverse scattering problem. The proposed inversion technique is applied to the measured experimental data to recover both shape and relative permittivity of unknown objects. The obtained results confirm the effectiveness of this algorithm and show that one can identify optimal parameters for the reference wave and an optimal procedure that results in good reconstructions of a penetrable, strongly scattering permittivity distribution
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