Comital Ireland, 1333–1534

The history of late-medieval Ireland is not exactly littered with dates that command general recognition, so it is surely suggestive that two which have achieved a degree of notoriety concern the fortunes, or rather misfortunes, of Ireland’s earls and earldoms: the murder of William Burgh, the ‘brown’ earl of Ulster, in 1333; and the rebellion in 1534 of Thomas Fitzgerald (‘Silken Thomas’), soon-to-be tenth earl of Kildare. These are dates of demarcation. In the broadest terms, 1333 has been understood to mark the end of the expansion of royal power under the Plantagenets, 1534 the start of its vigorous reassertion under the Tudors. What occurred between these chronological bookends? For Goddard Orpen (d. 1932), writing in 1920 when the Anglo-Irish tradition he cherished seemed imperilled by the prospect of Irish secession from the United Kingdom, the murder of the earl of Ulster in 1333 was a moment of dark, almost metonymic, significance: ‘the door was now closed on a century and a half of remarkable progress, vigour, and comparative order, and two centuries of retrogression, stagnation, and comparative anarchy were about to be ushered in’

Exercise and the microbiota

The authors are supported in part by research grants from Science Foundation Ireland including a centre grant (Alimentary Pharmabiotic Centre; Grant Numbers SFI/12/RC/2273 and 12/RC/2273). Dr. Orla O’Sullivan is funded by a Starting Investigator Research Grant from Science Foundation Ireland (Grant number 13/SIRG/2160). Dr. Paul Cotter is funded by a Principal Investigator Award from Science Foundation Ireland P.D.C are supported by a SFI PI award (Grant number 11/PI/1137).peer-reviewedSedentary lifestyle is linked with poor health, most commonly obesity and associated disorders, the corollary being that exercise offers a preventive strategy. However, the scope of exercise biology extends well beyond energy expenditure and has emerged as a great ‘polypill’, which is safe, reliable and cost-effective not only in disease prevention but also treatment. Biological mechanisms by which exercise influences homeostasis are becoming clearer and involve multi-organ systemic adaptations. Most of the elements of a modern lifestyle influence the indigenous microbiota but few studies have explored the effect of increased physical activity. While dietary responses to exercise obscure the influence of exercise alone on gut microbiota, professional athletes operating at the extremes of performance provide informative data. We assessed the relationship between extreme levels of exercise, associated dietary habits and gut microbiota composition, and discuss potential mechanisms by which exercise may exert a direct or indirect influence on gut microbiota.The authors are supported in part by research grants from Science Foundation Ireland including a centre grant (Alimentary Pharmabiotic Centre; Grant Numbers SFI/12/RC/2273 and 12/RC/2273). Dr. Orla O’Sullivan is funded by a Starting Investigator Research Grant from Science Foundation Ireland (Grant number 13/SIRG/2160). Dr. Paul Cotter is funded by a Principal Investigator Award from Science Foundation Ireland P.D.C are supported by a SFI PI award (Grant number 11/PI/1137)

Comparison of milk production from clover-based and fertilizer-N-based grassland

peer-reviewedFunding for this study was provided by the Irish National Development Plan and the Irish Dairy Levy.This study, conducted over four years (2003–2006), compared herbage production, nutritive value of herbage, the length of the grazing season and milk production per cow and per hectare from grassland systems based on (i) white clover (average 219 g/kg of herbage DM) (WC) receiving on average N application of 90 kg/ha (s.d. 6.4) in spring and successive 0.2 of the area over-seeded annually with white clover seed and (ii) fertilizer N (FN) input of 226 kg/ha (s.d. 9.7). The stocking density of Holstein- Friesian dairy cows on both systems was 2.0/ha 2003 and 2.2/ha in each of the following three years. There were 22 cows per system in 2003 and 24 cows per system in each of the following three years. Cows calved within a 12 week interval in spring with mean calving date in mid-February. Milk was produced until mid-December each year. Total annual herbage DM production was lower (P 0.05) differences in the in vitro organic matter digestibilities of pre-grazing herbage. The crude protein concentration in pre-grazing herbage DM was higher (P 0.05) differences in annual production of milk per cow (mean 6524 kg; s.e. 83.9 kg), live-weight or body condition score between the two systems. There were no (P < 0.05) differences in the lengths of the grazing season, which averaged 254 days (s.e. 0.9). Although there was no difference in performance per cow, the higher herbage production indicates that a higher stocking rate and milk output per hectare was possible from FN than WC. Nevertheless, the WC swards supported an annual stocking density of 2.15/ha and a milk output of 14 t/ha.National Development PlanIrish Dairy Levy Research Fun

Prevalence of BoHV-1 seropositive and BVD virus positive bulls on Irish dairy farms and associations between bull purchase and herd status

peer-reviewedBackground BVD and IBR are contagious viral diseases highly prevalent in Irish cattle. Despite their significant reproductive and economic impact very little is known about the BVD and IBR status of stock bulls (a bull used for breeding purposes). There are still a high proportion of dairy farms in Ireland that rely on the use of a bull for breeding cattle and ensuring the fertility of the bulls is of paramount importance for the efficiency of the farms. The prevalence of BoHV-1 and BVD in stock bulls in Irish dairy herds has never been investigated. The objectives of this study therefore were: (i) to provide descriptive, observational data on the use of stock bulls on Irish dairy farms; (ii) to investigate the BVD and BoHV1 status of a sub-set of stock bulls; (iii) to investigate factors associated with BVD and BoHV1 status of stock bulls and (iv) to investigate factors associated with dairy herd status for BVD and BoHV1, including any associations with the use of stock bull. A total of 529 blood samples from bulls involved in the dairy breeding process were analysed for BVD virus using RT-PCR, and BoHV-1 antibodies by ELISA test. A total of 305 different dairy herds took part in the study and the overall BVD and BoHV-1 herd status was determined by ELISA using four bulk tank milk samples over the 2009 lactation. Logistic regression was used to investigate the associations between the stock bulls and BVD and BoHV-1 herd and individual status. Results Of the 305 total participating farms, 235 farms (77 %) had at least one bull and 167 farms had purchased bulls. Two bulls (0.4 %) out of 529 tested were found positive for BVD virus and 87 (16.7 %) tested seropositive for BoHV-1. Some significant associations were identified between the purchase of bulls and both viral diseases. Purchased bulls were three times more likely to be seropositive for BoHV-1 than homebred bulls. In the same way, herds with purchased bulls were three times more likely to be classified as seropositive for BVD and four times more likely to have evidence of recent BoHV-1 circulation than farms where all the bulls were homebred. Conclusions The prevalence of BoHV-1 and BVD in stock bulls in Irish dairy herds has never been investigated. This study highlights the widespread use of stock bulls in Irish dairy herds, as well as the high rate of exchange of bulls between farms. Significant associations were found between the origin of the bull and their serological BoHV-1 status. In keeping with these results, bulls with higher number movements between farms were more likely to be seropositive for BoHV-1

Lactobacillus ruminis strains cluster according to their mammalian gut source

peer-reviewedBackground Lactobacillus ruminis is a motile Lactobacillus that is autochthonous to the human gut, and which may also be isolated from other mammals. Detailed characterization of L. ruminis has previously been restricted to strains of human and bovine origin. We therefore sought to expand our bio-bank of strains to identify and characterise isolates of porcine and equine origin by comparative genomics. Results We isolated five strains from the faeces of horses and two strains from pigs, and compared their motility, biochemistry and genetic relatedness to six human isolates and three bovine isolates including the type strain 27780T. Multilocus sequence typing analysis based on concatenated sequence data for six individual loci separated the 16 L. ruminis strains into three clades concordant with human, bovine or porcine, and equine sources. Sequencing the genomes of four additional strains of human, bovine, equine and porcine origin revealed a high level of genome synteny, independent of the source animal. Analysis of carbohydrate utilization, stress survival and technological robustness in a combined panel of sixteen L. ruminis isolates identified strains with optimal survival characteristics suitable for future investigation as candidate probiotics. Under laboratory conditions, six human isolates of L. ruminis tested were aflagellate and non-motile, whereas all 10 strains of bovine, equine and porcine origin were motile. Interestingly the equine and porcine strains were hyper-flagellated compared to bovine isolates, and this hyper-flagellate phenotype correlated with the ability to swarm on solid medium containing up to 1.8% agar. Analysis by RNA sequencing and qRT-PCR identified genes for the biosynthesis of flagella, genes for carbohydrate metabolism and genes of unknown function that were differentially expressed in swarming cells of an equine isolate of L. ruminis. Conclusions We suggest that Lactobacillus ruminis isolates have potential to be used in the functional food industry. We have also identified a MLST scheme able to distinguish between strains of L. ruminis of different origin. Genes for non-digestible oligosaccharide metabolism were identified with a putative role in swarming behaviour.This work was supported by a Principal Investigator Award (07/IN.1/B1780) from Science Foundation Ireland to P.W. O’Toole

Associations of sedentary behaviour, physical activity, blood pressure and anthropometric measures with cardiorespiratory fitness in children with cerebral palsy

Background - Children with cerebral palsy (CP) have poor cardiorespiratory fitness in comparison to their peers with typical development, which may be due to low levels of physical activity. Poor cardiorespiratory fitness may contribute to increased cardiometabolic risk. Purpose - The aim of this study was to determine the association between sedentary behaviour, physical activity and cardiorespiratory fitness in children with CP. An objective was to determine the association between cardiorespiratory fitness, anthropometric measures and blood pressure in children with CP. Methods- This study included 55 ambulatory children with CP [mean (SD) age 11.3 (0.2) yr, range 6-17 yr; Gross Motor Function Classification System (GMFCS) levels I and II]. Anthropometric measures (BMI, waist circumference and waist-height ratio) and blood pressure were taken. Cardiorespiratory fitness was measured using a 10 m shuttle run test. Children were classified as low, middle and high fitness according to level achieved on the test using reference curves. Physical activity was measured by accelerometry over 7 days. In addition to total activity, time in sedentary behaviour and light, moderate, vigorous, and sustained moderate-to-vigorous activity (≥10 min bouts) were calculated. Results - Multiple regression analyses revealed that vigorous activity (β = 0.339, p<0.01), sustained moderate-to-vigorous activity (β = 0.250, p<0.05) and total activity (β = 0.238, p<0.05) were associated with level achieved on the shuttle run test after adjustment for age, sex and GMFCS level. Children with high fitness spent more time in vigorous activity than children with middle fitness (p<0.05). Shuttle run test level was negatively associated with BMI (r2 = -0.451, p<0.01), waist circumference (r2 = -0.560, p<0.001), waist-height ratio (r2 = -0.560, p<0.001) and systolic blood pressure (r2 = -0.306, p<0.05) after adjustment for age, sex and GMFCS level. Conclusions - Participation in physical activity, particularly at a vigorous intensity, is associated with high cardiorespiratory fitness in children with CP. Low cardiorespiratory fitness is associated with increased cardiometabolic risk

Study protocol; thyroid hormone replacement for untreated older adults with subclinical hypothyroidism - a randomised placebo controlled trial (TRUST)

Background: Subclinical hypothyroidism (SCH) is a common condition in elderly people, defined as elevated serum thyroid-stimulating hormone (TSH) with normal circulating free thyroxine (fT4). Evidence is lacking about the effect of thyroid hormone treatment. We describe the protocol of a large randomised controlled trial (RCT) of Levothyroxine treatment for SCH. Methods: Participants are community-dwelling subjects aged ≥65 years with SCH, diagnosed by elevated TSH levels (≥4.6 and ≤19.9 mU/L) on a minimum of two measures ≥ three months apart, with fT4 levels within laboratory reference range. The study is a randomised double-blind placebo-controlled parallel group trial, starting with levothyroxine 50 micrograms daily (25 micrograms in subjects &lt;50Kg body weight or known coronary heart disease) with titration of dose in the active treatment group according to TSH level, and a mock titration in the placebo group. The primary outcomes are changes in two domains (hypothyroid symptoms and fatigue / vitality) on the thyroid-related quality of life questionnaire (ThyPRO) at one year. The study has 80% power (at p = 0.025, 2-tailed) to detect a change with levothyroxine treatment of 3.0% on the hypothyroid scale and 4.1% on the fatigue / vitality scale with a total target sample size of 750 patients. Secondary outcomes include general health-related quality of life (EuroQol), fatal and non-fatal cardiovascular events, handgrip strength, executive cognitive function (Letter Digit Coding Test), basic and instrumental activities of daily living, haemoglobin, blood pressure, weight, body mass index and waist circumference. Patients are monitored for specific adverse events of interest including incident atrial fibrillation, heart failure and bone fracture. Discussion: This large multicentre RCT of levothyroxine treatment of subclinical hypothyroidism is powered to detect clinically relevant change in symptoms / quality of life and is likely to be highly influential in guiding treatment of this common condition. Trial registration: Clinicaltrials.gov NCT01660126; registered 8th June 2012

A comparison of pilot-scale supersonic direct steam injection to conventional steam infusion and tubular heating systems for the heat treatment of protein-enriched skim milk-based beverages

peer-reviewedDirect supersonic steam injection, direct steam infusion, and indirect tubular heating were each applied to protein-enriched skim milk-based beverages with 4, 6 and 8% (w/w) total protein, and the effect of final heat temperature on the physical properties of these beverages was investigated. Supersonic steam injection resulted in significantly lower levels of denaturation of β-lactoglobulin (34.5%), compared to both infusion (76.3%) and tubular (97.1%) heating technologies. Viscosity, particle size and accelerated physical stability of formulations did not differ significantly between the heating technologies, while noticeable colour differences due to heat treatment (mainly attributed to increasing b* value) were observed, particularly for tubular heating. Overall, the extent of protein denaturation in high-protein dairy products was significantly influenced by the particular heating technology applied. The application of supersonic steam injection technology, with rapid heating and high shear characteristics, may enable differenciated product characteristics for ready-to-drink ambient-delivery high-protein dairy beverages. Industrial relevance: The design and application of novel direct supersonic steam injection technology was comprehensively studied and found to provide significant benefits over direct steam infusion and indirect tubular heating technologies for skim milk-based protein beverages. This type of injection heating system resulted in heat-treated formulations with lower levels of denatured whey proteins, compared to tubular and infusion heating, offering an alternative opportunity to the industry in terms of producing shelf-stable dairy protein beverages

A review of pellets from different sources

El aumento en el consumo de pellets se ha traducido en una amplia variedad de materiales para la fabricación de pellets. Por lo tanto, la industria de pellets ha empezado a buscar productos alternativos, tales como desechos de actividades agrícolas, de silvicultura y de las industrias afines, junto con la combinación de los mismos, obteniendo una amplia gama de estos productos. Además, la entrada en vigor de la norma EN ISO 17225 en el mercado de pellets de madera hace (entre otros tipos) posible para la industria y el uso doméstico. Por lo tanto, los desechos que son adecuados para el uso de la biomasa han aumentado recientemente. En este estudio, se han analizado las principales características de pelotillas de diez clases, hechas en laboratorios a partir de diferentes materias primas. Así, nos hemos centrado en los factores limitantes de la mayoría de los estándares de calidad que determinan la idoneidad para el mercado de biomasa, dependiendo del tipo de sedimento. Los resultados mostraron diferencias considerables entre las pastillas analizadas, excediendo los límites establecidos por el estándar en casi todos los casos, especialmente en lo que se refiere a contenido de cenizas y N y S la composición. Los requisitos de la norma estudiada, muy exigentes en ciertos factores, desactivar la entrada de estos desechos densificado en mercados de mayor valor añadido.The rise in pellet consumption has resulted in a wider variety of materials for pellet manufacture. Thus, pellet industry has started looking for alternative products, such as wastes from agricultural activities, forestry and related industries, along with the combination thereof, obtaining a broad range of these products. In addition, the entry into force of EN ISO 17225 standard makes wood pellet market (among other types) possible for industry and household purposes. Therefore, wastes that are suitable for biomass use have recently increased. In this study, the main characteristics of ten kinds of laboratory-made pellets from different raw materials were analyzed. Thus, we have focused on the most limiting factors of quality standards that determine the suitability for biomass market, depending on the kind of pellet. The results showed considerable differences among the analyzed pellets, exceeding the limits established by the standard in almost all cases, especially concerning ash content and N and S composition. The requirements of the studied standard, very demanding for certain factors, disable the entry of these densified wastes in greater added value markets.• Gobierno de Extremadura. Proyectos PCJ100502 y PCJ100603 • Gobierno de Extremadura. Ayuda a grupos de investigación GR10151peerReviewe