EFFECT OF PLATELET RICH PLASMA ON PROLIFERATION OF ENDOTHELIAL PROGENITOR CELL (EPC) OF STABLE CORONARY ARTERY DISEASE PATIENT

ABSTRACT Effect of Platelet Rich Plasma (PRP) on Proliferation of Endothelial Progenitor Cell (EPC) of Stable Coronary Artery Disease Patient Ronald R Hehanusa, Andrianto, Budi S Pikir   Background : Endothelial Progenitor Cell (EPC) is the progenitor of endothelial cell which has important role in  regulation of vascular wall integrity and homeostasis, to protect vessels from inflamation and thrombosis, that leads into pathogenesis of coronary artery disease. Growth factors proven has important role to stimulate transduction signal in the process of proliferation of EPC. Platelet Rich Plasma (PRP) contains variety of growth factors, wellknown role in homeostasis and wound healing process. Therefore, this study was conducted to analyze the effect of PRP on proliferation of EPC of Stable Coronary Artery Disease (SCAD) patient. Objective : To analyze the effect of Platelet Rich Plasma (PRP) on the proliferation of Endothelial Progenitor Cell (EPC) from peripheral blood of patient with SCAD    Methods : This is an in vitro, true experimental, post-test only control group design. The mononuclear cells were isolated from peripheral blood of SCAD patient and cultured in M-199 medium. EPC divided into 3 groups, which received Platelet Rich Plasma (PRP), Platelet Poor Plasma (PPP), and control. After 14 days of incubation, immunocytochemical examination was performed, EPC which marked with CD34, FITC labeled,was counted using immunofluoroscence microscope. Data analysis using ANOVA test. Result : Cell counting showed significant increase of EPC proliferation in PRP group compared to PPP group (1.052 ± 0.16 vs 0.762 ± 0.19, p = 0.003), and control group as well (1.052 ± 0.16 vs 0.068 ± 0.05, p = 0.000). EPC proliferation in PPP group also increase significantly compared to control group (0.762 ± 0.19 vs 0.068 ± 0.05, p = 0.000). Conclusion : Platelet Rich Plasma (PRP) increase EPC proliferation significantly from peripheral blood of SCAD patient.   Keywords : EPC proliferation, PRP, SCA

Analisis hukum Islam terhadap transaksi Jasa Platelet Rich Plasma (PRP) Treatment di Klinik Cholista Skincare Surabaya

Skripsi ini adalah hasil penelitian lapangan untuk menjawab pertanyaan bagaimana praktik transaksi jasa Platelet Rich Plasma (PRP) antara pihak Mu’ajir atau penyewa jasa yang disini disebut pasien dengan Musta’jir atau pemberi jasa yang disini adalah seorang dokter sekaligus pemilik klinik Cholista Skincare Surabaya dan bagaimana kajian Ijarah terhadap praktik jasa Platelet Rich Plasma (PRP) di Klinik Cholista Skincare Surabaya. Metode penelitian yang digunakan dalam penelitian ini adalah kualitatif, dimana pengumpulan data diperoleh melalui dokumentasi dan wawancara. Kemudian, data disusun dan dianalisis menggunakan metode deskriptif analisis yakni menggambarkan atau mendeskripsikan data yang telah diperoleh dari lapangan terkait praktik Platelet Rich Plasma (PRP) Treatment, dimana mengemukakan hasil penelitian yang ada di klinik Cholista Skincare Surabaya, untuk kemudia data tersebut di analisisdengan teori hukum Islam tentang Ijarah untuk dapat diketahui jawaban dan ditarik kesimpulan. Dari hasil penelitian dapat disimpulkan bahwa, pertama praktik Platelet Rich Plasma (PRP) Treatment adalah salah satu perawatan kecantikan kulit yang menggunakan darah sebagai bahan dasar untuk di oleskan pada wajah pasien. Dalam hal ini, darah merupakan zat yang najis. Dalam pengupahannya sang pasien membayar uang sebesar satu juta rupiah setelah melakukan treatment. Dalam praktik Platelet Rich Plasma (PRP) Treatment rukun dan syarat dari objeknya tidak sesuai dalam akad Ijarah yang mensyaratkan sesuatu pekerjaan atau barang yang disewa yang harus dibolehkan secara agama. Kedua, dalam fatwa Fatwa MUI Nomor 26 Tahun 2013 Tentang Standar Kehalalan Produk Kosmetik dan {Penggunaannya yang menyatakan bahwa bahan yang digunakan harus halal dan suci. Sehingga, Ujrah yang diterima pun hukumnya haram. Setelah dilakukannya penelitian ini, maka peneliti memberi saran kepada pemberi jasa (Musta’jir) dan kepada pihak penyewa jasa (Mu’ajir). Bahwasannya harus benar-benar mempertimbangkan lagi apakah tidak ada pilihan perawatan lain yang memberikan efek yang sama seperti yang dihasilkan oleh perawatan Platelet Rich Plasma (PRP) ini. Mengingat karena kecantikan bukanlah hal dharurat dan bahan dasar darah yang digunakan untuk treatment ini merupakan zat yang haram

PLATELET RICH PLASMA (PRP) MANFAAT ANTI-DIABETIK

Buku ini mengupas segala bentuk manfaat dari Platelet Rich Plasma (PRP) sebagai antihiperglikemik pada penyakit Diabetes melitus. PRP banyak dikenal di berbagai bidang kedokteran, dikarenakan PRP itu sendiri memiliki banyak manfaat. Buku ini menejelaskan secara mendalam peran dari PRP sebagai anti-hiperglikemik terhadap penyakit Diabetes melitus denganpenelitian in-vivo

Comparative outcomes of platelet concentrates and blood clot scaffolds for regenerative endodontic procedures : a systematic review of randomized controlled clinical trials

The main objective of this systematic review is to evaluate the effectiveness of platelet concentrates -Platelet-rich plasma (PRP) or Fibrin-rich plasma (PRF)- compared with blood clot (BC) as scaffolds for maturogenesis, in patients with immature perman

Platelet-Rich-Plasma (PRP) treatment for equine osteoarthritis

PòsterJun

Indications of Platelet-Rich Plasma (PRP) in Dermatology

Introduction: PRP is an autologous serum containing high concentrations of platelets and growth factors. It is used in dermatology for a variety of purposes, including hair growth, skin renewal, the removal of acne scars, dermal augmentation, and the treatment of striae distensae. The origins of PRP trace back to the 1970s, originating within the field of hematology as a treatment for patients grappling with thrombocytopenia. The subsequent decades, specifically the 1980s and 1990s, witnessed the integration of PRP into surgical procedures, notably in maxillofacial and plastic surgeries. The rationale underlying the employment and therapeutic potential of a concentrated platelet solution hinges upon their remarkable ability to furnish elevated levels of vital growth factors, thus instigating a regenerative impetus that facilitates repair in tissues characterized by limited innate healing capabilities. This comprehensive review delves into the multifaceted facets of PRP, encompassing mechanisms of action, prevailing indications, existing clinical substantiation, safety considerations, and the burgeoning spectrum of potential applications within the domain of skin condition treatments. Aim of the study: To review the current literature on the use of PRP in the field of dermatology Materials and Methods: We reviewed the literature available in PubMed, using the key words “platelet rich plasma”, “prp”, “autologous platelet rich plasma”, “alopecia”, “anti-aging” Results: PRP has a lot of potential in dermatology and its therapeutic uses are growing. Future research should standardize PRP therapy procedures for particular reasons. Summary: The versatile action of PRP has made it the subject of intense research. However, the reports in the literature to date on its use require further studies on larger groups of people to confirm its effects

EVALUATION OF PLATELET RICH PLASMA (PRP) PREPARATION PROCEDURE

The success of PRP therapy in repairing tissue damage is influenced by the PRP preparation procedure. Currently, there’s no standardization of PRP preparation procedures, and various techniques are used, such as the use of anticoagulants and different centrifugation speeds. This study aimed to evaluating the PRP preparation procedures based on the centrifugations steps (single and double centrifugation) and the use of  anticoagulants variation (sodium citrate, EDTA and ACD-A). This study was an experimental study and used blood samples from respondents. The selected respondents must meet inclusion and exclusion criteria. The treatment groups in this study were the single centrifugation group and the double centrifugation group. Each group will be divided into 3 subgroups with different anticoagulant usage (sodium citrate, EDTA and ACD-A). Statistical analysis results showed a significant difference in the mean platelet count in the sodium citrate , EDTA, ACD-A groups with single and double centrifugation steps. Evaluation of platelet preparation procedures in this study, a higher platelet count was obtained, specifically in the sodium citrate group (494 x 103 cells/µL), EDTA group (829.4 x 103 cells/µL), and ACD-A group (607.1 x 103 cells/µL), compared to single centrifugation in the sodium citrate group (354.8 x 103 cells/µL), EDTA group (408.1 x 103 cells/µL), and ACD-A group (390.6 x 103 cells/µL). The highest platelet count in PRP was achieved with the preparation procedure using EDTA as the anticoagulant with double centrifugation. Further research is necessary to evaluate PRP preparation procedures regarding the concentration of growth factors present in PRP

Effects of platelet rich plasma (PRP) on human gingival fibroblast, osteoblast and periodontal ligament cell behaviour

The use of platelet rich plasma (PRP, GLO) has been used as an adjunct to various regenerative dental procedures. The aim of the present study was to characterize the influence of PRP on human gingival fibroblasts, periodontal ligament (PDL) cells and osteoblast cell behavior in vitro

Platelet-rich plasma in clinical practice

The concept of using a patient’s own blood or components thereof (autologous), to enhance the physiological process of healing has been in place for many years. Autologous platelet-rich plasma (PRP) has been used for both soft tissue and bone healing and rejuvenation. PRP has also been used in orthopaedics for bone, tendon and muscle injuries, dentistry for dental implants, dermatology for wound healing, and in pathological conditions such as alopecia aereata. However, more recently, it has been used in the fast-growing field of aesthetic and anti-ageing medicine for skin rejuvenation. PRP seems like a logical, safe, relatively, cheap and easy procedure, but is this the case? Although safety and improved short-term outcomes for orthopaedic indications have been demonstrated in a few reviews, long-term improvement has not been demonstrated. Randomised controlled trials in dermatology and aesthetic indications are sparse, but show promise for alopecia areata and skin rejuvenation.Keywords: autologous, indications, platelet-rich plasma, PRP, skin rejuvenatio

An animal experimental study on pubourethral ligament restoration with platelet rich plasma for the treatment of stress urinary incontinence.

Introduction: Minimally invasive methods for injured ligament and tendon restoration have been developed and gained popularity in recent years. Injury and relaxation of the pubourethral ligament (PUL) can lead to stress urinary incontinence (SUI). The aim of this study was to investigate the impact of injecting platelet rich plasma (PRP) into the PUL following its surgical transection resulting in SUI, confirmed by leak point pressure (LPP) measurements pre- and post-intervention in an experimental animal model. Material and methods: Twenty female adult Sprague-Dawley rats were assigned in 2 groups: 1) treatment group with transection of the PUL and application of PRP at the time of transection and at one month follow-up and 2) a control group, with transection of the PUL only. Leak point pressures (LPPs) were measured prior to transection, immediately following the transection and at 1 and 2 months in both groups. Results: The median LPPs for the control group were: LPP - preT: 35.6 (29.8-44.8) cmH2O, LPP - postT: 14.6 (5.8-19.0) cmH2O, LPP - 1 month: 27.3 (19.2-33.8) cmH2O, LPP - 2 months: 29.0 (27.0-34.0) cmH2O, whereas for the PRP group were: LPP-preT: 40.5 (33.2-46.3) cmH2O, LPP - postT: 15.7 (3.0-24.0) cmH2O, LPP - 1month: 31.6 (24.8-37.4) cmH2O, LPP - 2 months: 36.8 (32.5-45.4) cmH2O. PRP injections on transected PULs significantly increased LPPs at one month follow-up [31.6 cmH2O vs. 27.3 cmH2O, p = .043]. This effect was confirmed at two months [36.8 cmH2O vs. 29.0 cmH2O, p <.001]. Conclusions: Injection of PRP into transected PULs significantly improved LPPs at one and two months' follow-up. However, further experimental and clinical research is needed to evaluate the safety and efficacy of this treatment, in clinical practice