Characteristics of lightning flashes generating dancing sprites above thunderstorms

During the night of October 29-30, 2013, a low-light video camera at Pic du Midi (2877 m) in the French Pyrénées, recorded TLEs above a very active storm over the Mediterranean Sea. The minimum cloud top temperature reached -73 °C at ~1600 UTC while its cloud to ground (CG) flash rate reached ~30 fl min-1. Some sprite events with long duration are classified as dancing sprites. We analyze in detail the temporal evolution and estimated location of sprite elements for two cases of these events. They consist in series of sprite sequences with a duration that exceeds 1 second. By associating the cloud structure, the lightning activity, the electric field radiated in a broad range of low frequencies and the current moment waveform of the lightning strokes, some findings are highlighted: (i) In each series, successive sprite sequences reflect the occurrence time and location of individual positive lightning strokes across the stratiform region. (ii) The longer time-delayed (> 20 ms) sprite elements correspond to the lower impulsive charge moment changes (iCMC) of the parent stroke (< 200 C km) and they are shifted few tens of kilometres from their SP+CG stroke. However, both short and long time-delayed sprite elements also occur after strokes that produce a large iCMC and that are followed by a continuing current. (iii) The long time-delayed sprite elements produced during the continuing current correspond to surges in the current moment waveform. They occur sometimes at an altitude apparently lower than the previous short time-delayed sprite elements, possibly because of the lowered altitude of the ionosphere potential. (iv) The largest and brightest sprite elements produce significant current signatures, visible when their delay is not too short (~3-5 ms).Preprin

Effect of the Monocyte Chemoattractant Protein-1/CC Chemokine Receptor 2 System on Nephrin Expression in Streptozotocin-Treated Mice and Human Cultured Podocytes

OBJECTIVE-Monocyte chemoattractant protein-1 (MCP-1), a chemokine binding to the CC chemokine receptor 2 (CCR2) and promoting monocyte infiltration, has been implicated in the pathogenesis of diabetic nephropathy. To assess the potential relevance of the MCP-1/CCR2 system in the pathogenesis of diabetic proteinuria, we studied in vitro if MCP-1 binding to the CCR2 receptor modulates nephrin expression in cultured podocytes. Moreover, we investigated in vivo if glomerular CCR2 expression is altered in kidney biopsies from patients with diabetic nephropathy and whether lack of MCP-1 affects proteinuria and expression of nephrin in experimental diabetes. RESEARCH DESIGN AND METHODS-Expression of nephrin was assessed in human podocytes exposed to rh-MCP-1 by immunofluorescence and real-time PCR. Glomerular CCR2 expression was studied in 10 kidney sections from patients with overt nephropathy and eight control subjects by immunohistochemistry. Both wild-type and MCP-1 knockout mice were made diabetic with streptozotocin. Ten weeks after the onset of diabetes, albuminuria and expression of nephrin, synaptopodin, and zonula occludens-1 were examined by immunofluorescence and immunoblotting. RESULTS-In human podocytes, MCP-1 binding to the CCR2 receptor induced a significant reduction in nephrin both mRNA and protein expression via a Rho-dependent mechanism. The MCP-1 receptor, CCR2, was overexpressed in the glomerular podocytes of patients with overt nephropathy. In experimental diabetes, MCP-1 was overexpressed within the glomeruli and the absence of MCP-1 reduced both albuminuria and downregulation of nephrin and synaptopodin. CONCLUSIONS-These findings suggest that the MCP-1/CCR2 system may be relevant in the pathogenesis of proteinuria in diabetes

Sperm Swimming Speeds In The Eastern Oyster Crassostrea Virginica (Gmelin, 1791)

Oysters, like the vast majority of sessile marine invertebrates, shed sperm and eggs into the water column where fertilization subsequently occurs. The fate of the gametes depends on their passive movements at various scales in a high-viscosity environment, the longevity of the sperm\u27s ability to affect oriented movement, the rate of sperm movement toward the egg target, and the ability of sperm to effect fertilization. Oyster sperm swim in a helical pattern with a mean forward progression velocity of 0.057 +/- 0.010 mm/sec (SE; n = 25) with the 95 percentile range extending from 0.036-0.078 mm/sec, a value comparable with that reported for echinoderm sperm

Dancing sprites:detailed analysis of two case studies

On 29–30 October 2013, a low-light video camera installed at Pic du Midi (2877¿m), recorded transient luminous events above a very active storm over the Mediterranean Sea. The minimum cloud top temperature reached -73°C, while its cloud to ground (CG) flash rate exceeded 30¿fl¿min-1. Some sprite events have long duration and resemble to dancing sprites. We analyze in detail the temporal evolution and estimated location of two series of sprite sequences, as well as the cloud structure, the lightning activity, the electric field radiated in a broad range of low frequencies, and the current moment waveform of the lightning strokes. (i) In each series, successive sprite sequences reflect time and location of corresponding positive lightning strokes across the stratiform region. (ii) The longer time-delayed (>20¿ms) sprite elements correspond to the lower impulsive charge moment changes (iCMC) of the parent strokes (<200¿C¿km), and they are shifted few tens of kilometers from their SP¿+¿CG stroke. However, both short and long time-delayed sprite elements also occur after strokes that produce a large iCMC and that are followed by a continuing current. (iii) The long time-delayed sprite elements during the continuing current correspond to surges in the current moment waveform. They occur sometimes at an altitude apparently lower than the previous short time-delayed sprite elements, possibly because of changes in the local conductivity. (iv) The largest and brightest sprite elements produce significant current signatures, visible when their delay is not too short (~3–5¿ms).Peer ReviewedPreprin

Relativistic electron beams above thunderclouds

Non-luminous relativistic electron beams above thunderclouds have been detected by the radio signals of low frequency &amp;sim;40–400 kHz which they radiate. The electron beams occur &amp;sim;2–9 ms after positive cloud-to-ground lightning discharges at heights between &amp;sim;22–72 km above thunderclouds. Intense positive lightning discharges can also cause sprites which occur either above or prior to the electron beam. One electron beam was detected without any luminous sprite which suggests that electron beams may also occur independently of sprites. Numerical simulations show that beams of electrons partially discharge the lightning electric field above thunderclouds and thereby gain a mean energy of &amp;sim;7 MeV to transport a total charge of &amp;sim;−10 mC upwards. The impulsive current &amp;sim;3 &amp;times; 10&lt;sup&gt;&amp;minus;3&lt;/sup&gt; Am&lt;sup&gt;−2&lt;/sup&gt; associated with relativistic electron beams above thunderclouds is directed downwards and needs to be considered as a novel element of the global atmospheric electric circuit

Electron backscatter diffraction in materials characterization

Electron Back-Scatter Diffraction (EBSD) is a powerful technique that captures electron diffraction patterns from crystals, constituents of material. Captured patterns can then be used to determine grain morphology, crystallographic orientation and chemistry of present phases, which provide complete characterization of microstructure and strong correlation to both properties and performance of materials. Key milestones related to technological developments of EBSD technique have been outlined along with possible applications using modern EBSD system. Principles of crystal diffraction with description of crystallographic orientation, orientation determination and phase identification have been described. Image quality, resolution and speed, and system calibration have also been discussed. Sample preparation methods were reviewed and EBSD application in conjunction with other characterization techniques on a variety of materials has been presented for several case studies. In summary, an outlook for EBSD technique was provided

Molecular and Cellular Basis of Microvascular Perfusion Deficits Induced by Clostridium perfringens and Clostridium septicum

Reduced tissue perfusion leading to tissue ischemia is a central component of the pathogenesis of myonecrosis caused by Clostridium perfringens. The C. perfringens α-toxin has been shown capable of inducing these changes, but its potential synergy with perfringolysin O (θ-toxin) is less well understood. Similarly, Clostridium septicum is a highly virulent causative agent of spontaneous gas gangrene, but its effect on the microcirculation has not been examined. Therefore, the aim of this study was to use intravital microscopy to examine the effects of C. perfringens and C. septicum on the functional microcirculation, coupled with the use of isogenic toxin mutants to elucidate the role of particular toxins in the resultant microvascular perfusion deficits. This study represents the first time this integrated approach has been used in the analysis of the pathological response to clostridial toxins. Culture supernatants from wild-type C. perfringens induced extensive cell death within 30 min, as assessed by in vivo uptake of propidium iodide. Furthermore, significant reductions in capillary perfusion were observed within 60 min. Depletion of either platelets or neutrophils reduced the alteration in perfusion, consistent with a role for these blood-borne cells in obstructing perfusion. In addition, mutation of either the α-toxin or perfringolysin O structural genes attenuated the reduction in perfusion, a process that was reversed by genetic complementation. C. septicum also induced a marked reduction in perfusion, with the degree of microvascular compromise correlating with the level of the C. septicum α-toxin. Together, these data indicate that as a result of its ability to produce α-toxin and perfringolysin O, C. perfringens rapidly induces irreversible cellular injury and a marked reduction in microvascular perfusion. Since C. septicum induces a similar reduction in microvascular perfusion, it is postulated that this function is central to the pathogenesis of clostridial myonecrosis, irrespective of the causative bacterium

An approach to ground based space surveillance of geostationary on-orbit servicing operations

AbstractOn Orbit Servicing (OOS) is a class of dual-use robotic space missions that could potentially extend the life of orbiting satellites by fuel replenishment, repair, inspection, orbital maintenance or satellite repurposing, and possibly reduce the rate of space debris generation. OOS performed in geostationary orbit poses a unique challenge for the optical space surveillance community. Both satellites would be performing proximity operations in tight formation flight with separations less than 500m making atmospheric seeing (turbulence) a challenge to resolving a geostationary satellite pair when viewed from the ground. The two objects would appear merged in an image as the resolving power of the telescope and detector, coupled with atmospheric seeing, limits the ability to resolve the two objects. This poses an issue for obtaining orbital data for conjunction flight safety or, in matters pertaining to space security, inferring the intent and trajectory of an unexpected object perched very close to one׳s satellite asset on orbit. In order to overcome this problem speckle interferometry using a cross spectrum approach is examined as a means to optically resolve the client and servicer׳s relative positions to enable a means to perform relative orbit determination of the two spacecraft. This paper explores cases where client and servicing satellites are in unforced relative motion flight and examines the observability of the objects. Tools are described that exploit cross-spectrum speckle interferometry to (1) determine the presence of a secondary in the vicinity of the client satellite and (2) estimate the servicing satellite׳s motion relative to the client. Experimental observations performed with the Mont Mégantic 1.6m telescope on co-located geostationary satellites (acting as OOS proxy objects) are described. Apparent angular separations between Anik G1 and Anik F1R from 5 to 1 arcsec were observed as the two satellites appeared to graze one another. Data reduction using differential angular measurements derived from speckle images collected by the 1.6m telescope produced relative orbit estimates with better than 90m accuracy in the cross-track and in-track directions but exhibited highly variable behavior in the radial component from 50 to 1800m. Simulations of synthetic tracking data indicated that the radial component requires approximately six hours of tracking data for an Extended Kalman Filter to converge on an relative orbit estimate with less than 100m overall uncertainty. The cross-spectrum approach takes advantage of the Fast Fourier Transform (FFT) permitting near real-time estimation of the relative orbit of the two satellites. This also enables the use of relatively larger detector arrays (>106 pixels) helping to ease acquisition process to acquire optical angular data