Web Content Extraction - a Meta-Analysis of its Past and Thoughts on its Future

In this paper, we present a meta-analysis of several Web content extraction algorithms, and make recommendations for the future of content extraction on the Web. First, we find that nearly all Web content extractors do not consider a very large, and growing, portion of modern Web pages. Second, it is well understood that wrapper induction extractors tend to break as the Web changes; heuristic/feature engineering extractors were thought to be immune to a Web site's evolution, but we find that this is not the case: heuristic content extractor performance also tends to degrade over time due to the evolution of Web site forms and practices. We conclude with recommendations for future work that address these and other findings.Comment: Accepted for publication in SIGKDD Exploration

Sonoluminescence: Nature's Smallest BlackBody

The Spectrum of the light emitted by a sonoluminescing bubble is extremely well fit by the spectrum of a blackbody. Furthermore the radius of emission can be smaller than the wavelength of the light. Consequences, for theories of sonoluminescence are discussed.Comment: 8 pages, 3 Figure

Effect of fire induced water repellency on soil hydraulic properties and water flow

Water infiltration into the root zone, its retention in soil and drainage from the soil profile, are highly sensitive to the presence, degree and persistence of soil water repellency (SWR). Prolonged drought periods and wildfires can increase SWR substantially, thus    the aim of this study was to determine the effect of forest fire-induced water repellency    on soil hydraulic properties, infiltration and water flow in unsaturated soil (vadose) zone. Infiltration experiments with water and ethanol were conducted on forest sites, selected according to their exposure to fire: heavily burned (A), burned (B) and non-affected as the control site (C). Infiltration data were used as an input for inverse determination of soil hydraulic parameters required for computer model calibration (HYDRUS 2D/3D). Then, a one-year climatic scenario for 2016 with measured meteorological data was simulated using HYDRUS-1D software. Data showed that in the case of soil exposure to high temperatures (forest fires), a relatively large increase of SWR is observed. Compared to the control plot, a considerably greater difference between the hydraulic conductivity, Ks, values for water and ethanol was found at both fire affected plots. This suggested positive relationship between soil water repellency and reduced water infiltration. Numerical simulation of the intensive (extreme) rainfall event clearly showed that SWR affects soil water balance by reducing the infiltration and increasing the surface runoff

CXCL12 Mediates CCR7-independent Homing of Central Memory Cells, But Not Naive T Cells, in Peripheral Lymph Nodes

Central memory CD8+ T cells (TCM) confer superior protective immunity against infections compared with other T cell subsets. TCM recirculate mainly through secondary lymphoid organs, including peripheral lymph nodes (PLNs). Here, we report that TCM, unlike naive T cells, can home to PLNs in both a CCR7-dependent and -independent manner. Homing experiments in paucity of lymph node T cells (plt/plt) mice, which do not express CCR7 ligands in secondary lymphoid organs, revealed that TCM migrate to PLNs at ∼20% of wild-type (WT) levels, whereas homing of naive T cells was reduced by 95%. Accordingly, a large fraction of endogenous CD8+ T cells in plt/plt PLNs displayed a TCM phenotype. Intravital microscopy of plt/plt subiliac lymph nodes showed that TCM rolled and firmly adhered (sticking) in high endothelial venules (HEVs), whereas naive T cells were incapable of sticking. Sticking of TCM in plt/plt HEVs was pertussis toxin sensitive and was blocked by anti-CXCL12 (SDF-1α). Anti-CXCL12 also reduced homing of TCM to PLNs in WT animals by 20%, indicating a nonredundant role for this chemokine in the presence of physiologic CCR7 agonists. Together, these data distinguish naive T cells from TCM, whereby only the latter display greater migratory flexibility by virtue of their increased responsiveness to both CCR7 ligands and CXCL12 during homing to PLN

Automatic Discovery of Political Meme Genres with Diverse Appearances

Forms of human communication are not static -- we expect some evolution in the way information is conveyed over time because of advances in technology. One example of this phenomenon is the image-based meme, which has emerged as a dominant form of political messaging in the past decade. While originally used to spread jokes on social media, memes are now having an outsized impact on public perception of world events. A significant challenge in automatic meme analysis has been the development of a strategy to match memes from within a single genre when the appearances of the images vary. Such variation is especially common in memes exhibiting mimicry. For example, when voters perform a common hand gesture to signal their support for a candidate. In this paper we introduce a scalable automated visual recognition pipeline for discovering political meme genres of diverse appearance. This pipeline can ingest meme images from a social network, apply computer vision-based techniques to extract local features and index new images into a database, and then organize the memes into related genres. To validate this approach, we perform a large case study on the 2019 Indonesian Presidential Election using a new dataset of over two million images collected from Twitter and Instagram. Results show that this approach can discover new meme genres with visually diverse images that share common stylistic elements, paving the way forward for further work in semantic analysis and content attribution.Comment: 13 pages, 14 figure

Differential effects of α4β7 and GPR15 on homing of effector and regulatory T cells from patients with UC to the inflamed gut in vivo

Objective: Gut homing of lymphocytes via adhesion molecules has recently emerged as new target for therapy in inflammatory bowel diseases. We aimed to analyze the in vivo homing of effector (Teff) and regulatory (Treg) T cells to the inflamed gut via α4β7 and GPR15. Design: We assessed the expression of homing receptors on T cells in peripheral blood and inflamed mucosa. We studied the migration pattern and homing of Teff and Treg cells to the inflamed gut using intravital confocal microscopy and FACS in a humanized mouse model in DSS-treated NSG (NOD.Cg-Prkdcscid-Il2rgtm1Wjl/SzJ) mice. Results: Expression of GPR15 and α4β7 was significantly increased on Treg rather than Teff cells in peripheral blood of patients with ulcerative colitis (UC) as compared to Crohn´s disease and controls. In vivo analysis in a humanized mouse model showed augmented gut homing of UC Treg cells as compared to controls. Moreover, suppression of UC (but not control) Teff and Treg cell homing was noted upon treatment with the α4β7 antibody vedolizumab. In contrast, siRNA blockade of GPR15 had only effects on homing of Teff cells but did not affect Treg homing in UC. Clinical vedolizumab treatment was associated with marked expansion of UC Treg cells in peripheral blood. Conclusion: α4β7 rather than GPR15 is crucial for increased colonic homing of UC Treg cells in vivo, while both receptors control UC Teff homing. Vedolizumab treatment impairs homing of UC Treg cells leading to their accumulation in peripheral blood with subsequent suppression of systemic effector T cell expansion

Correlated Multimodal Imaging in Life Sciences:Expanding the Biomedical Horizon

International audienceThe frontiers of bioimaging are currently being pushed toward the integration and correlation of several modalities to tackle biomedical research questions holistically and across multiple scales. Correlated Multimodal Imaging (CMI) gathers information about exactly the same specimen with two or more complementary modalities that-in combination-create a composite and complementary view of the sample (including insights into structure, function, dynamics and molecular composition). CMI allows to describe biomedical processes within their overall spatio-temporal context and gain a mechanistic understanding of cells, tissues, diseases or organisms by untangling their molecular mechanisms within their native environment. The two best-established CMI implementations for small animals and model organisms are hardware-fused platforms in preclinical imaging (Hybrid Imaging) and Correlated Light and Electron Microscopy (CLEM) in biological imaging. Although the merits of Preclinical Hybrid Imaging (PHI) and CLEM are well-established, both approaches would benefit from standardization of protocols, ontologies and data handling, and the development of optimized and advanced implementations. Specifically, CMI pipelines that aim at bridging preclinical and biological imaging beyond CLEM and PHI are rare but bear great potential to substantially advance both bioimaging and biomedical research. CMI faces three mai

Exogenous WNT5A and WNT11 proteins rescue CITED2 dysfunction in mouse embryonic stem cells and zebrafish morphants

Mutations and inadequate methylation profiles of CITED2 are associated with human congenital heart disease (CHD). In mouse, Cited2 is necessary for embryogenesis, particularly for heart development, and its depletion in embryonic stem cells (ESC) impairs cardiac differentiation. We have now determined that Cited2 depletion in ESC affects the expression of transcription factors and cardiopoietic genes involved in early mesoderm and cardiac specification. Interestingly, the supplementation of the secretome prepared from ESC overexpressing CITED2, during the onset of differentiation, rescued the cardiogenic defects of Cited2-depleted ESC. In addition, we demonstrate that the proteins WNT5A and WNT11 held the potential for rescue. We also validated the zebrafish as a model to investigate cited2 function during development. Indeed, the microinjection of morpholinos targeting cited2 transcripts caused developmental defects recapitulating those of mice knockout models, including the increased propensity for cardiac defects and severe death rate. Importantly, the co-injection of anti-cited2 morpholinos with either CITED2 or WNT5A and WNT11 recombinant proteins corrected the developmental defects of Cited2-morphants. This study argues that defects caused by the dysfunction of Cited2 at early stages of development, including heart anomalies, may be remediable by supplementation of exogenous molecules, offering the opportunity to develop novel therapeutic strategies aiming to prevent CHD.Agência financiadora: Fundação para a Ciência e a Tecnologia (FCT) Comissão de Coordenação e Desenvolvimento Regional do Algarve (CCDR Algarve) ALG-01-0145-FEDER-28044; DFG 568/17-2 Algarve Biomedical Center (ABC) Municipio de Louléinfo:eu-repo/semantics/publishedVersio