Policy and Practice Brief 4: The Obligation of Schools to Provide Information to Multilingual Families in a Language They Can Understand

Research shows that strong relationships between families and schools are key to student success. By providing information and communication in languages and formats that multilingual parents and guardians can access and understand, schools can strengthen relationships with these families. This policy and practice brief provides research and resources on this topic

Teachers\u27 Perceptions of the Broad Validity of a High Stakes English Language Proficiency Test

The No Child Left Behind Act of 2001 mandated that the English acquisition of all students identified as English learners be assessed annually using high-stakes standardized English language proficiency tests, and the Every Student Succeeds Act of 2015 continues this testing mandate. The WIDA ACCESS for ELLs® has been used for this and other purposes since 2005, and has been adopted by 40 State Education Agencies, including the Virginia Department of Education. However, despite the long-standing and widespread use of this assessment, no comprehensive independent evaluations of the test have been conducted. This mixed-methods study is a conceptual replication of a previous study evaluating the validity of a similar high-stakes standardized English language proficiency test. Using a Broad Validity Framework that considered the test’s reliability, criterion validity, and consequential validity, the study surveyed and interviewed Virginia teachers of English as a second language on their perspectives on the test. Findings suggest that while some teachers believe some sort of test is warranted for accountability and informing decisions, there are many threats to the validity of decisions based on test scores, including potential sources of construct-irrelevant variance, issues with technology associated with the online version of the test, the lapse of time between test administration and the receipt of scores, and questions regarding student motivation and test-taking effort. Furthermore, the study suggests the test has unintended consequences, including negative emotional impacts for teachers and students and a loss of instructional time. Because of questions raised regarding the reliability and validity of the test, study findings suggest the use of multiple measures in high-stakes decision-making for English learners. Furthermore, findings affirm the value of a consideration of teacher input in test evaluations

Teachers\u27 Efforts to Support Undocumented Students Within Ambiguous Policy Contexts

Although education scholars have recently focused greater attention on the experiences of undocumented youth in schools, few studies have examined educators\u27 perceptions of their roles and responsibilities with regards to this population. Since the 1982 Supreme Court decision Plyler v. Doe guaranteed education to this group and barred schools from inquiring about immigration status, little additional policy has offered guidance on how schools can support this group while also refraining from identifying it\u27s members. Policies are particularly lacking in new destination areas where there are fewer resources and less infrastructure for new immigrant populations. As increasingly harsh immigration enforcement policies amplify fear and anxiety among families, educators and other service providers are more in need of support than perhaps ever before. Some teachers in new destination areas, however, have found ways to create safe and empowering spaces for undocumented students. We conducted a qualitative case study to explore how such educators understand their roles amidst both this policy void and a political climate in which immigration has become an especially contentious and divisive issue. We interviewed 18 teachers of immigrant students in one new destination area in Virginia, a state experiencing an increase in the undocumented population. We found that teachers took actions to enhance students\u27 feelings of security and normalcy through curricular decisions, emotional support, and even the provision of basic needs. Teacher\u27s actions, which were often spontaneous, adaptive, and resourceful, could be described as agile advocacy. These actions ranged from providing information to families to pushing for school and district policy changes. However, the teachers also encountered many barriers and few supports in these efforts. They felt largely alone and frustrated by the incomprehensibility of the immigration system and the absence of building-and district-level leadership in support of undocumented students

Policy and Practice Brief 7: Special Considerations for ACCESS Testing During COVID-19

Because of the COVID-19 pandemic, in-person instruction in Virginia public schools was suspended in March 2020 for the remainder of the school year. In 2020-2021, Virginia schools offered an evolving range of virtual, hybrid, concurrent, and face-to-face instruction. Despite the challenges of schooling during the pandemic, federal high-stakes testing mandates remained in place in 2020-2021, and all students identified as English learners (ELs) were still required to take the annual assessment of English language proficiency (ELP), the WIDA ACCESS for ELLs©. Since this test cannot be administered remotely, ELs participating in virtual schooling were expected to return to school buildings solely to take the test, despite serious concerns for safety and test validity being raised by several educational and civil rights organizations. This mini-brief explains these concerns, and offers recommendations for schools confronting the challenges of administering the test and interpreting test results during the pandemic

Policy and Practice Brief 6: Considerations for Using ACCESS Test Scores in Decision-Making

Since the implementation of The No Child Left Behind Act of 2001 (NCLB), all students identified as English learners (ELs) in U.S. public schools have been required to take an annual assessment of English language proficiency (ELP) for federal accountability. Virginia and many other state education agencies (SEAs) use the WIDA ACCESS for ELLs© test (ACCESS), not only to comply with this mandate, but also to inform a variety of high-stakes decisions for EL instruction, such as determining when multilingual learners have reached English proficiency, deciding about Language Instruction Educational Program (LIEP) entry and exit, informing classroom instruction and assessment, monitoring year-to-year student progress, deciding on staffing levels, and evaluating teachers. Despite the test’s long history, widespread adoption, multiple uses, and costs, few independent evaluations of the test have been conducted; however, study of Virginia EL teachers raised questions about the reliability of ACCESS and the validity of decisions based on the test scores. In light of these questions, considerations and recommendations for the administration of ACCESS and use of scores in high-stakes decision-making are presented

Methicillin Resistance Alters the Biofilm Phenotype and Attenuates Virulence in Staphylococcus aureus Device-Associated Infections

Clinical isolates of Staphylococcus aureus can express biofilm phenotypes promoted by the major cell wall autolysin and the fibronectin-binding proteins or the icaADBC-encoded polysaccharide intercellular adhesin/poly-N-acetylglucosamine (PIA/PNAG). Biofilm production in methicillin-susceptible S. aureus (MSSA) strains is typically dependent on PIA/PNAG whereas methicillin-resistant isolates express an Atl/FnBP-mediated biofilm phenotype suggesting a relationship between susceptibility to β-lactam antibiotics and biofilm. By introducing the methicillin resistance gene mecA into the PNAG-producing laboratory strain 8325-4 we generated a heterogeneously resistant (HeR) strain, from which a homogeneous, high-level resistant (HoR) derivative was isolated following exposure to oxacillin. The HoR phenotype was associated with a R602H substitution in the DHHA1 domain of GdpP, a recently identified c-di-AMP phosphodiesterase with roles in resistance/tolerance to β-lactam antibiotics and cell envelope stress. Transcription of icaADBC and PNAG production were impaired in the 8325-4 HoR derivative, which instead produced a proteinaceous biofilm that was significantly inhibited by antibodies against the mecA-encoded penicillin binding protein 2a (PBP2a). Conversely excision of the SCCmec element in the MRSA strain BH1CC resulted in oxacillin susceptibility and reduced biofilm production, both of which were complemented by mecA alone. Transcriptional activity of the accessory gene regulator locus was also repressed in the 8325-4 HoR strain, which in turn was accompanied by reduced protease production and significantly reduced virulence in a mouse model of device infection. Thus, homogeneous methicillin resistance has the potential to affect agr- and icaADBC-mediated phenotypes, including altered biofilm expression and virulence, which together are consistent with the adaptation of healthcare-associated MRSA strains to the antibiotic-rich hospital environment in which they are frequently responsible for device-related infections in immuno-compromised patients

A Gene Catalogue of the Euchromatic Male-Specific Region of the Horse Y Chromosome: Comparison with Human and Other Mammals

Studies of the Y chromosome in primates, rodents and carnivores provide compelling evidence that the male specific region of Y (MSY) contains functional genes, many of which have specialized roles in spermatogenesis and male-fertility. Little similarity, however, has been found between the gene content and sequence of MSY in different species. This hinders the discovery of species-specific male fertility genes and limits our understanding about MSY evolution in mammals. Here, a detailed MSY gene catalogue was developed for the horse – an odd-toed ungulate. Using direct cDNA selection from horse testis, and sequence analysis of Y-specific BAC clones, 37 horse MSY genes/transcripts were identified. The genes were mapped to the MSY BAC contig map, characterized for copy number, analyzed for transcriptional profiles by RT-PCR, examined for the presence of ORFs, and compared to other mammalian orthologs. We demonstrate that the horse MSY harbors 20 X-degenerate genes with known orthologs in other eutherian species. The remaining 17 genes are acquired or novel and have so far been identified only in the horse or donkey Y chromosomes. Notably, 3 transcripts were found in the heterochromatic part of the Y. We show that despite substantial differences between the sequence, gene content and organization of horse and other mammalian Y chromosomes, the functions of MSY genes are predominantly related to testis and spermatogenesis. Altogether, 10 multicopy genes with testis-specific expression were identified in the horse MSY, and considered likely candidate genes for stallion fertility. The findings establish an important foundation for the study of Y-linked genetic factors governing fertility in stallions, and improve our knowledge about the evolutionary processes that have shaped Y chromosomes in different mammalian lineages

Meta-analysis of pharmacogenetic interactions in amyotrophic lateral sclerosis clinical trials

OBJECTIVE: To assess whether genetic subgroups in recent amyotrophic lateral sclerosis (ALS) trials responded to treatment with lithium carbonate, but that the treatment effect was lost in a large cohort of nonresponders. METHODS: Individual participant data were obtained from 3 randomized trials investigating the efficacy of lithium carbonate. We matched clinical data with data regarding the UNC13A and C9orf72 genotype. Our primary outcome was survival at 12 months. On an exploratory basis, we assessed whether the effect of lithium depended on the genotype. RESULTS: Clinical data were available for 518 of the 606 participants. Overall, treatment with lithium carbonate did not improve 12-month survival (hazard ratio [HR] 1.0, 95% confidence interval [CI] 0.7-1.4; p = 0.96). Both the UNC13A and C9orf72 genotype were independent predictors of survival (HR 2.4, 95% CI 1.3-4.3; p = 0.006 and HR 2.5, 95% CI 1.1-5.2; p = 0.032, respectively). The effect of lithium was different for UNC13A carriers (p = 0.027), but not for C9orf72 carriers (p = 0.22). The 12-month survival probability for UNC13A carriers treated with lithium carbonate improved from 40.1% (95% CI 23.2-69.1) to 69.7% (95% CI 50.4-96.3). CONCLUSIONS: This study incorporated genetic data into past ALS trials to determine treatment effects in a genetic post hoc analysis. Our results suggest that we should reorient our strategies toward finding treatments for ALS, start focusing on genotype-targeted treatments, and standardize genotyping in order to optimize randomization and analysis for future clinical trials

Embracing monogenic Parkinson's disease: the MJFF Global Genetic PD Cohort

© 2023 The Authors. Movement Disorders published by Wiley Periodicals LLC on behalf of International Parkinson and Movement Disorder Society. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.Background: As gene-targeted therapies are increasingly being developed for Parkinson's disease (PD), identifying and characterizing carriers of specific genetic pathogenic variants is imperative. Only a small fraction of the estimated number of subjects with monogenic PD worldwide are currently represented in the literature and availability of clinical data and clinical trial-ready cohorts is limited. Objective: The objectives are to (1) establish an international cohort of affected and unaffected individuals with PD-linked variants; (2) provide harmonized and quality-controlled clinical characterization data for each included individual; and (3) further promote collaboration of researchers in the field of monogenic PD. Methods: We conducted a worldwide, systematic online survey to collect individual-level data on individuals with PD-linked variants in SNCA, LRRK2, VPS35, PRKN, PINK1, DJ-1, as well as selected pathogenic and risk variants in GBA and corresponding demographic, clinical, and genetic data. All registered cases underwent thorough quality checks, and pathogenicity scoring of the variants and genotype-phenotype relationships were analyzed. Results: We collected 3888 variant carriers for our analyses, reported by 92 centers (42 countries) worldwide. Of the included individuals, 3185 had a diagnosis of PD (ie, 1306 LRRK2, 115 SNCA, 23 VPS35, 429 PRKN, 75 PINK1, 13 DJ-1, and 1224 GBA) and 703 were unaffected (ie, 328 LRRK2, 32 SNCA, 3 VPS35, 1 PRKN, 1 PINK1, and 338 GBA). In total, we identified 269 different pathogenic variants; 1322 individuals in our cohort (34%) were indicated as not previously published. Conclusions: Within the MJFF Global Genetic PD Study Group, we (1) established the largest international cohort of affected and unaffected individuals carrying PD-linked variants; (2) provide harmonized and quality-controlled clinical and genetic data for each included individual; (3) promote collaboration in the field of genetic PD with a view toward clinical and genetic stratification of patients for gene-targeted clinical trials. © 2023 The Authors. Movement Disorders published by Wiley Periodicals LLC on behalf of International Parkinson and Movement Disorder Society.Michael J. Fox Foundation for Parkinson's Research. Grant Number: ID 15015.02. NIHR Cambridge Biomedical Research Centre. Grant Number: BRC-1215-20014info:eu-repo/semantics/publishedVersio

Prevalence and architecture of de novo mutations in developmental disorders.

The genomes of individuals with severe, undiagnosed developmental disorders are enriched in damaging de novo mutations (DNMs) in developmentally important genes. Here we have sequenced the exomes of 4,293 families containing individuals with developmental disorders, and meta-analysed these data with data from another 3,287 individuals with similar disorders. We show that the most important factors influencing the diagnostic yield of DNMs are the sex of the affected individual, the relatedness of their parents, whether close relatives are affected and the parental ages. We identified 94 genes enriched in damaging DNMs, including 14 that previously lacked compelling evidence of involvement in developmental disorders. We have also characterized the phenotypic diversity among these disorders. We estimate that 42% of our cohort carry pathogenic DNMs in coding sequences; approximately half of these DNMs disrupt gene function and the remainder result in altered protein function. We estimate that developmental disorders caused by DNMs have an average prevalence of 1 in 213 to 1 in 448 births, depending on parental age. Given current global demographics, this equates to almost 400,000 children born per year