8 research outputs found
Mechanical Properties of Molybdenum Disulfide and the Effect of Doping: An in Situ TEM Study
Direct observations on nanopillars
composed of molybdenum disulfide
(MoS<sub>2</sub>) and chromium-doped MoS<sub>2</sub> and their response
to compressive stress have been made. Time-resolved transmission electron
microscopy (TEM) during compression of the submicrometer diameter
pillars of MoS<sub>2</sub>- and Cr-doped MoS<sub>2</sub> (Cr: 0, 10,
and 50 at %) allow the deformation process of the material to be observed
and can be directly correlated with mechanical response to applied
load. The addition of chromium to the MoS<sub>2</sub> changed the
failure mode from plastic deformation to catastrophic brittle fracture,
an effect that was more pronounced as chromium content increased
A roadmap for gene system development in Clostridium
Clostridium species are both heroes and villains. Some cause serious human and animal diseases, those present in the microbiota contribute to health and wellbeing, while others represent useful industrial chassis for the production of chemicals and fuels. To understand, counter or exploit, there is a fundamental requirement for effective systems that may be used for directed or random genome modifications. We have formulated a simple roadmap whereby the necessary gene systems maybe developed and deployed. At its heart is the use of 'pseudo-suicide' vectors and the creation of a pyrE mutant (a uracil auxotroph), initially aided by ClosTron technology, but ultimately made using a special form of allelic exchange termed ACE (Allele-Coupled Exchange). All mutants, regardless of the mutagen employed, are made in this host. This is because through the use of ACE vectors, mutants can be rapidly complemented concomitant with correction of the pyrE allele and restoration of uracil prototrophy. This avoids the phenotypic effects frequently observed with high copy number plasmids and dispenses with the need to add antibiotic to ensure plasmid retention. Once available, the pyrE host may be used to stably insert all manner of application specific modules. Examples include, a sigma factor to allow deployment of a mariner transposon, hydrolases involved in biomass deconstruction and therapeutic genes in cancer delivery vehicles. To date, provided DNA transfer is obtained, we have not encountered any clostridial species where this technology cannot be applied. These include, Clostridium difficile, Clostridium acetobutylicum, Clostridium beijerinckii, Clostridium botulinum, Clostridium perfringens, Clostridium sporogenes, Clostridium pasteurianum, Clostridium ljungdahlii, Clostridium autoethanogenum and even Geobacillus thermoglucosidasius
Formation and Characterization of Model Iron Sulfide Scales with Disulfides and Thiols on Steel Pipeline Materials by an Aerosol Assisted Chemical Vapor Method
Mechanical Properties of Molybdenum Disulfide and the Effect of Doping: An in Situ TEM Study
Mechanical Properties of Molybdenum Disulfide and the Effect of Doping: An in Situ TEM Study
Thin Films of Molybdenum Disulfide Doped with Chromium by Aerosol-Assisted Chemical Vapor Deposition (AACVD)
A combined single-source precursor
approach has been developed
for the deposition of thin films of Cr-doped molybdenum disulfide
(MoS<sub>2</sub>) by aerosol-assisted chemical vapor deposition (AACVD).
TrisÂ(diethyldithiocarbamato)ÂchromiumÂ(III) can also be used for the
deposition of chromium sulfide (CrS). Films have been analyzed by
a range of techniques including scanning electron microscopy (SEM),
energy dispersive X-ray (EDX) spectroscopy, Raman spectroscopy, and
powder X-ray diffraction (pXRD) to elucidate film morphology, composition,
and crystallinity. The presence of Cr in the MoS<sub>2</sub> films
produces a number of striking morphological, crystallographic, and
nanomechanical changes to the deposited films. The chromium dopant
appears to be uniform throughout the MoS<sub>2</sub> from the scanning
transmission electron microscopy (STEM) EDX spectrum imaging of nanosheets
produced by liquid-phase exfoliation of the thin films in <i>N</i>-methyl-2-pyrollidone