Sensory characterization of juice obtained via rehydration of freeze-dried and spray-dried grapefruit

This is the peer reviewed version of the following article: Martínez Navarrete, Nuria, Camacho Vidal, Mª Mar, Agudelo-Sterling, Claudia, Salvador Alcaraz, Ana. (2018). Sensory characterization of juice obtained via rehydration of freeze-dried and spray-dried grapefruit.Journal of the Science of Food and Agriculture, 99, 1, 244-252. DOI: 10.1002/jsfa.9166, which has been published in final form at http://doi.org/10.1002/jsfa.9166. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving[EN] BACKGROUND The acceptability of grapefruit juice obtained from rehydrated freeze-dried and spray-dried fruit was studied and compared with that of natural freshly squeezed juice and that of a commercial juice. The rheological properties, colour and solute concentration were determined. A sensory evaluation was developed to evaluate juice acceptability. Furthermore, a penalty analysis was used to determine the attributes with improvement potential as well as some CATA (check all that apply) questions, where consumers marked the most characteristic attributes of each sample. RESULTS CONCLUSION The rehydrated samples were different from the commercial and natural juices. When grapefruit is freeze-dried or spray-dried, gum arabic and bamboo fibre are added and part of the fruit pulp is present; this is not so in the other juices under study. This supposes greater viscosity, luminosity and chroma together with a reddish tone of the rehydrated juices, especially the freeze-dried ones. It also affects their acidity, astringency and sweetness. Rehydrating the freeze-dried or spray-dried powder to the level of the natural juice, instead of that of the crushed or liquidized grapefruit, and incorporating sugar at this stage would probably improve the quality of the samples. (c) 2018 Society of Chemical IndustryThe authors thank the Ministerio de Economia y Competitividad for the financial support given through the Project AGL 2012-39103. We would also like to thank to Mike Bennet for assistance in correcting the English manuscript.Martínez Navarrete, N.; Camacho Vidal, MM.; Agudelo-Sterling, C.; Salvador Alcaraz, A. (2018). Sensory characterization of juice obtained via rehydration of freeze-dried and spray-dried grapefruit. Journal of the Science of Food and Agriculture. 99(1):244-252. https://doi.org/10.1002/jsfa.916624425299

Estudo clinico-epidemiológico de 27 envenenamentos causados pela ingestão de baiacus (Tetrodontidae) nos estados de Santa Catarina e Bahia, Brasil

Os baiacus ou peixes-bola podem ser venenosos devido à presença em seus tecidos corporais de Tetrodotoxina e/ou Saxitoxina, ambas potentes neurotoxinas. Os autores relatam 27 envenenamentos por ingestão da carne de baiacus. Os pacientes foram atendidos nos Centros de Toxicologia de Santa Catarina e da Bahia de 1984 a 2009. Os acidentes foram classificados em moderados (52%) e graves (33%), havendo dois óbitos. O diagnóstico precoce é fundamental no sentido de garantir suporte ventilatório aos pacientes.Puffer fish can be poisonous due to the presence of the potent neurotoxins such as Tetrodotoxin (TTX) and Saxitoxin (STX) found in its tissues. The authors report 27 human poisonings from ingestion of puffer fish in patients treated at Toxicology Centers in the states of Santa Catarina and Bahia, Brazil, between 1984 and January 2009. Poisonings were classified as moderate (52%) and severe (33%), two deaths were observed. Early diagnosis is very important to ensure respiratory support

A cidade (in)segura e o impacto na produção da subjetividade de moradores da zona norte do Rio de Janeiro: um estudo exploratório

A literatura vigente tem apontado a violência como um problema de saúde pública, afetando a vida dos cidadãos, marcando-os com sentimentos de insegurança, medo e vulnerabilidade. O objetivo deste estudo é investigar que repercussões o fenômeno da insegurança tem gerado na produção de subjetividades, e como este sentimento pode influenciar o cotidiano dos indivíduos. Para obtenção dos dados foi utilizado um roteiro de entrevista semiestruturado, com 24 pessoas entrevistadas, de ambos os sexos, residentes da zona norte da cidade do Rio de Janeiro. A partir da análise do conteúdo das respostas colhidas, constatou-se que existe uma percepção distinta entre homens e mulheres a respeito da segurança/insegurança, mudanças nos hábitos cotidianos devido ao sentimento de insegurança, e criação de estratégias para lidar com esta problemática. Concluiu-se que embora cada um tenha uma percepção de sua realidade e do ambiente que os cerca, todos são afetados pelo fenômeno da violência

Evaluating students' perception of Scrum through a learning game

Games for learning help students and professionals to incorporate new knowledge through a playful experience. With the popularization of virtual education (partially due to COVID-19), there is a need for new tools that complement virtual educational environments. To deal with this need, we present Scrum Game, a mobile application game that aims to support the Scrum software methodology teaching and training. Scrum Game offers 42 activities grouped in levels with specific learning goals. This paper reports on a controlled experiment that evaluates Scrum Game in a course with more than 160 students. We assessed Scrum Game's effectiveness in terms of (i) user performance (i.e., students' marks and number of levels/activities completed) and (ii) user experience (i.e., usability). We found that students who used Scrum Game outperformed those who did not use it. We also found that students think that Scrum Game exhibits an enjoyable user experience.Sociedad Argentina de Informática e Investigación Operativ

Cell sources of inflammatory mediators present in bone marrow areas inside the meniscus

Copyright: © 2019 Rocha et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.Purpose: To demonstrate the production of inflammatory mediators by cells located in bone marrow spaces inside rodent menisci. Methods: Mice subjected to transection of the medial collateral and anterior cruciate ligaments and meniscotomy (osteoarthritis model) or to a sham procedure, as well as non-operated (naive) mice and rats, had knee joints excised. Tissues were stained with hematoxylin-eosin and tartrate-resistant acid phosphatase (TRAP). CD68+ cells, inducible nitric oxide synthase (iNOS), interleukin (IL)-1β, and tumor necrosis factor (TNF) expression were detected using immunohistochemistry. Results: Lamellar ossified areas, bone-entrapped osteocytes and bone marrow spaces were found inside menisci of one week up to 6 months-old naïve mice, regardless of gender. Menisci from naive rats also showed the same pattern with bone marrow areas. CD68+ cells were identified in bone marrow areas inside the meniscus of mice. TRAP+ osteoclasts, and hematogenous precursors expressing IL-1β, TNF, and iNOS were identified inside bone marrow areas in meniscal samples from both naïve and sham operated mice. Quantitative immunoexpression of IL-1 β, TNF and iNOS was more intense, P = 0.0194, 0.0293, 0.0124, respectively, in mouse knees from mice sacrificed 49 days after being subjected to an osteoarthritis (OA) model as compared to sham operated animals. Conclusion: We provide novel data showing that rodent menisci display bone marrow areas with cells able to produce inflammatory mediators. Immunoexpression of inflammatory mediators in those bone marrow areas is significantly more pronounced in mice subjected to experimental OA.The study was funded by: UID/BIM/50005/2019, project funded by Fundação para a Ciência e a Tecnologia (FCT)/ Ministério da Ciência, Tecnologia e Ensino Superior (MCTES) through Fundos do Orçamento de Estado.info:eu-repo/semantics/publishedVersio

RECURSOS DO AMBIENTE FAMILIAR E PARTICIPAÇÃO DOS PAIS NA VIDA ESCOLAR

Os pais têm um papel importante na aprendizagem escolar das crianças. Levando-se em consideração o papel de destaque do ambiente familiar e do envolvimento dos pais no desenvolvimento da criança, o presente estudo pretende identificar diferenças tanto nos recursos do ambiente familiar como naparticipação dos pais na vida escolar entrepais que têm filhos matriculados em escola pública ou em escola particular.Participaram desta pesquisa 60 mães, 31dessas mães têm os seus filhos matriculados na redepública de ensino e 29 têm os seus filhos matriculados em escola particular com idade entre 6 e 10 anos residentes na cidade do Rio deJaneiro. Foi utilizado o Inventário de Recursos do Ambiente Familiar e também foi aplicado um questionário com 6 perguntas com o objetivode verificar o envolvimento direto dos pais nas atividades escolares. Os resultados mostraram que as mães da escola particular identificaram mais recursos no ambiente familiar em relação aos brinquedos e à presença de livros. Em relação à supervisão e organização de rotinas, foram mais rigorosas no sentido de determinarem um horário certo para os seus filhos almoçarem. Por outro lado, as mães do grupo da escola pública identificaram uma maior frequência nas reuniões de família na hora do almoço. No que diz respeito à participação dos pais na vida escolar, não foi encontrada diferença estatisticamentesignificativa entre os grupos (p> 0,05). A partir deste estudo, pretende-se atuar na orientação e conscientização dos pais em relação a importância de acompanharem a vida escolar de seus filhos

Submarine Navigation using Neutrinos

Neutrinos are among the most abundant particles in the universe, nearly massless, travel at speeds near the speed of light and are electrically neutral. Neutrinos can be generated through man-made sources like particle accelerators or by natural sources like the sun. Neutrinos only interact via the weak force and gravity. Since gravitational interaction is extremely weak and the weak force has a very short range, neutrinos can travel long distances unimpeded through matter, reaching places inaccessible to GNSS (Global Navigation Satellite System) signals such as underwater locations. The main objective of this work is to sketch an early high-level design of a Neutrino PNT (Position, Navigation and Timing) mission and analyze its feasibility for submarine navigation since there is a need to improve current navigation technologies for submarines. The high-level preliminary concept proposes Cyclotrons or Linear Accelerators based on the physical process Pion Decay at Rest as neutrino sources. For detecting such isotropic neutrino fluxes user equipment must be composed of a high-performance clock synchronized with the system, a detector and possibly additional sensors such as IMU (Inertial Measurement Unit). A feasibility analysis of the recommended system option is performed based on simulations for determining the neutrino detection rate and on a PNT tool to estimate the PNT performances. Although the submarine navigation application is in the limit of being feasible with current technology, it could be realized with some important but reasonable progress in source and neutrino detector technology.Comment: 10pages, 12 figure

The Rapid Antigen Detection Test for SARS-CoV-2 Underestimates the Identification of COVID-19 Positive Cases and Compromises the Diagnosis of the SARS-CoV-2 (K417N/T, E484K, and N501Y) Variants

Timely detection of severe acute respiratory syndrome due to coronavirus 2 (SARS-CoV-2) by reverse transcription quantitative polymerase chain reaction (RT-qPCR) has been the gold- strategy for identifying positive cases during the current pandemic. However, faster and less expensive methodologies are also applied for the massive diagnosis of COVID-19. In this way, the rapid antigen test (RAT) is widely used. However, it is necessary to evaluate its detection efficiency considering the current pandemic context with the circulation of new viral variants. In this study, we evaluated the sensitivity and specificity of RAT (SD BIOSENSOR, South Korea), widely used for testing and SARS-CoV-2 diagnosis in Santiago of Chile. The RAT showed a 90% (amplification range of 20 ≤ Cq 30. In SARS-CoV-2 variant detection, RAT had a 42.8% detection sensitivity in samples with RT-qPCR amplification range 20 ≤ Cq <25 containing the single nucleotide polymorphisms (SNP) K417N/T, N501Y and E484K, associated with beta or gamma SARS-CoV-2 variants. This study alerts for the special attention that must be paid for the use of RAT at a massive diagnosis level, especially in the current scenario of appearance of several new SARS-CoV-2 variants which could generate false negatives and the compromise of possible viral outbreaks

TRAF3 alterations are frequent in del-3′IGH chronic lymphocytic leukemia patients and define a specific subgroup with adverse clinical features

Interstitial 14q32 deletions involving IGH gene are infrequent events in chronic lymphocytic leukemia (CLL), affecting less than 5% of patients. To date, little is known about their clinical impact and molecular underpinnings, and its mutational landscape is currently unknown. In this work, a total of 871 CLLs were tested for the IGH break-apart probe, and 54 (6.2%) had a 300 kb deletion of 3′IGH (del-3′IGH CLLs), which contributed to a shorter time to first treatment (TFT). The mutational analysis by next-generation sequencing of 317 untreated CLLs (54 del-3′IGH and 263 as the control group) showed high mutational frequencies of NOTCH1 (30%), ATM (20%), genes involved in the RAS signaling pathway (BRAF, KRAS, NRAS, and MAP2K1) (15%), and TRAF3 (13%) within del-3′IGH CLLs. Notably, the incidence of TRAF3 mutations was significantly higher in del-3′IGH CLLs than in the control group (p < .001). Copy number analysis also revealed that TRAF3 loss was highly enriched in CLLs with 14q deletion (p < .001), indicating a complete biallelic inactivation of this gene through deletion and mutation. Interestingly, the presence of mutations in the aforementioned genes negatively refined the prognosis of del-3′IGH CLLs in terms of overall survival (NOTCH1, ATM, and RAS signaling pathway genes) and TFT (TRAF3). Furthermore, TRAF3 biallelic inactivation constituted an independent risk factor for TFT in the entire CLL cohort. Altogether, our work demonstrates the distinct genetic landscape of del-3′IGH CLL with multiple molecular pathways affected, characterized by a TRAF3 biallelic inactivation that contributes to a marked poor outcome in this subgroup of patients.Funding information: Universidad de Salamanca; Fundación Española de Hematología y Hemoterapia (FEHH); Centro de Investigación Biomédica en Red de Cáncer (CIBERONC), Grant/Award Number: CB16/12/00233; Red Temática de Investigación Cooperativa en Cáncer (RTICC); “Fundación Memoria Don Samuel Solórzano Barruso”: FS/33–2020, Grant/Award Number: RD12/0036/0069; “Gerencia Regional de Salud, SACYL”:, Grant/Award Numbers: GRS2385/A/21, GRS2140/A/20; Consejería de Educación, Junta de Castilla y León, Grant/Award Number: SA118P20; European Regional Development Fund and Instituto de Salud Carlos III, Grant/Award Numbers: CD19/00222, FI19/00191; Spanish Fondo de Investigaciones Sanitarias, Grant/Award Numbers: PI21/00983, PI18/0150