Spectral Diagnostics of Active Prominences

Active prominences exhibit plasma motions, resulting in difficulties with the interpretation of spectroscopic observations. These solar features being strongly influenced by the radiation coming from the solar disk, Doppler dimming or brightening effects may arise, depending on which lines are observed and on the velocity of the plasma. Interlocking between the different atomic energy levels and non local thermodynamic equilibrium lead to non-trivial spectral line profiles, and this calls for complex numerical modelling of the radiative transfer in order to understand the observations. We present such a tool, which solves the radiative transfer and statistical equilibrium for H, He I, He II, and Ca II, in moving prominences where radial plasma motions are taking place. It is found that for isothermal, isobaric prominence models, the He II resonance lines are very sensitive to the Doppler effect and show a strong Doppler dimming. The Ca II lines are not very sensitive to the Doppler effect for the prominence models considered here. We illustrate how the code makes it possible to retrieve the plasma thermodynamic parameters by comparing computed and observed line profiles of hydrogen and helium resonance lines in a quiescent prominence.Comment: 6 pages, 5 figures. In press,"Physics of Chromospheric Plasmas" (Coimbra), ASP 368, 337 (2007). Revised version matches published version

The Helium spectrum in erupting solar prominences

Even quiescent solar prominences may become active and sometimes erupt. These events are occasionally linked to coronal mass ejections. However we know very little about the plasma properties during the activation and eruption processes. We present new computations of the helium line profiles emitted by an eruptive prominence. The prominence is modelled as a plane-parallel slab standing vertically above the solar surface and moving upward as a solid body. The helium spectrum is computed with a non local thermodynamic equilibrium radiative transfer code. The effect of Doppler dimming / brightening is investigated in the resonance lines of He I and He II formed in the EUV, as well as on the He I 10830 A and 5876 A lines. We focus on the line profile properties and the resulting integrated intensities. It is shown that the helium lines are very sensitive to Doppler dimming effects. We also study the effect of frequency redistribution in the formation mechanisms of the resonance lines and find that it is necessary to use partial redistribution in frequency for the resonance lines.Comment: 5 pages, 4 figures. Proceedings of IAU GA 2006, JD03: Solar Active Regions and 3D Magnetic Structure. See also a more detailed paper at astro-ph/060822

Flows at the Edge of an Active Region: Observation and Interpretation

Upflows observed at the edges of active regions have been proposed as the source of the slow solar wind. In the particular case of Active Region (AR) 10942, where such an upflow has been already observed, we want to evaluate the part of this upflow that actually remains confined in the magnetic loops that connect AR10942 to AR10943. Both active regions were visible simultaneously on the solar disk and were observed by STEREO/SECCHI EUVI. Using Hinode/EIS spectra, we determine the Doppler shifts and densities in AR10943 and AR10942, in order to evaluate the mass flows. We also perform magnetic field extrapolations to assess the connectivity between AR10942 and AR10943. AR10943 displays a persistent downflow in Fe XII. Magnetic extrapolations including both ARs show that this downflow can be connected to the upflow in AR10942. We estimate that the mass flow received by AR10943 areas connected to AR10942 represents about 18% of the mass flow from AR10942. We conclude that the upflows observed on the edge of active regions represent either large-scale loops with mass flowing along them (accounting for about one-fifth of the total mass flow in this example) or open magnetic field structures where the slow solar wind originates.Comment: 20 pages, 9 figures, accepted for publication in Astrophys.

A Time-Evolving 3D Method Dedicated to the Reconstruction of Solar plumes and Results Using Extreme Ultra-Violet Data

An important issue in the tomographic reconstruction of the solar poles is the relatively rapid evolution of the polar plumes. We demonstrate that it is possible to take into account this temporal evolution in the reconstruction. The difficulty of this problem comes from the fact that we want a 4D reconstruction (three spatial dimensions plus time) while we only have 3D data (2D images plus time). To overcome this difficulty, we introduce a model that describes polar plumes as stationary objects whose intensity varies homogeneously with time. This assumption can be physically justified if one accepts the stability of the magnetic structure. This model leads to a bilinear inverse problem. We describe how to extend linear inversion methods to these kinds of problems. Studies of simulations show the reliability of our method. Results for SOHO/EIT data show that we are able to estimate the temporal evolution of polar plumes in order to improve the reconstruction of the solar poles from only one point of view. We expect further improvements from STEREO/EUVI data when the two probes will be separated by about 60 degrees

STED-SPIM made simple

This work has been partially funded by the Agence Nationale de la Recherche under the program. ANR-13-NANO-0004-01. Teodora Scheul acknowledges a doctoral fellowship from the Nanosciences Foundation (Grenoble, France). We thank the company TEEMPhotonics (Meylan, France) for the loan of a laser.International audienceWe report the development of a stimulated emission depletion (STED) selective plane illumination (SPIM) microscope based on a single diode-pumped solid state (DPSS) laser that simultaneously delivers nanosecond-pulses at two wavelengths. The two wavelengths, 355 nm and 532 nm, are generated by harmonic conversion and they are used to induce respectively excitation and stimulated emission depletion. This source should allow a low-cost, compact, very efficient and simplified STED scheme since the two beams are intrinsically aligned and synchronized. Using a chromatic beam shaping device which leaves the excitation beam unaffected and produces a donut-shaped STED beam, we demonstrate a 300% reduction of the light sheet thickness, together with an enhancement of the sheet uniformity over larger field of view, at low STED power, in Coumarin dye solution. OCIS codes: (180.2520) Fluorescence microscopy; (180.0180) Microscopy. References and links 1. S. W. Hell and J. Wichmann, "Breaking the diffraction resolution limit by stimulated emission: stimulated-emission-depletion fluorescence microscopy," Opt. Lett. 19(11), 780–782 (1994). 2. M. Dyba and S. W. Hell, "Focal spots of size λ/23 open up far-field fluorescence microscopy at 33 nm axial resolution," Phys., "High-resolution three-dimensional imaging of large specimens with light sheet-based microscopy," Nat. Methods 4(4), 311–313 (2007). 8

Hydrogen Lyman-alpha and Lyman-beta radiances and profiles in polar coronal holes

The hydrogen Lyman-alpha plays a dominant role in the radiative energy transport in the lower transition region, and is important for the stud- ies of transition-region structure as well as solar wind origin. We investigate the Ly-alpha profiles obtained by SUMER in coronal holes and quiet Sun. In a subset of these observations, also the Hi Lyman-beta, Si iii, and O vi lines were (quasi-) simultaneously recorded. We find that the distances between the two peaks of Ly-alpha profiles are larger in coronal holes than in the quiet Sun, indicating a larger opacity in coronal holes. This difference might result from the different magnetic structures or the different radiation fields in the two regions. Most of the Ly-beta profiles in the coronal hole have a stronger blue peak, in contrast to those in quiet-Sun regions. Whilst in both regions the Ly-alpha profiles are stronger in the blue peak. Although the asymmetries are likely to be produced by differential flows in the solar atmosphere, their detailed formation processes are still unclear. The radiance ratio between Ly-alpha and Ly-beta decreases towards the limb in the coronal hole, which might be due to the different opacity of the two lines. We also find that the radiance distributions of the four lines are set by a combined effect of limb brightening and the different emission level between coronal holes and quiet Sun.Comment: 13 pages,4 figures, 1 talbe, accepted by Ap

Apports de la microscopie biphotonique intravitale pulmonaire à l'étude de la physiopathologie de la maladie du charbon

Bacillus anthracis, l'agent infectieux responsable de la maladie du charbon, est un agent pathogène majeur du risque biologique provoqué, notamment en raison de la sévérité de la forme respiratoire de la maladie. Celle-ci résulte de l'inhalation de spores dont les mécanismes de pénétration au niveau pulmonaire sont mal connus à l'heure actuelle. Cette thèse présente les apports des microscopies confocale et biphotonique à l'étude de ces mécanismes de pénétration des spores inhalées. Le modèle murin CX3CR1+/gfp, dont la sous-population CD11b+ de cellules dendritiques (DCs) exprime constitutivement la protéine de fluorescence verte (GFP), a été utilisé dans ces travaux. Une première partie présente le développement d'une méthode automatisée de discrimination des DCs parmi d'autres populations cellulaires exprimant le même fluorophore, en se basant sur le calcul d'un coefficient morphologique. Cette méthode a permis d'étudier dans un deuxième temps le comportement spécifique de la sous-population de DCs CD11b, après infection par des spores de B. anthracis. L'étude microscopique a été d'abord effectuée in situ, c'est-à-dire sur des explants pulmonaires maintenus dans des conditions favorables à la préservation de l'activité cellulaire, puis in vivo, sur des souris anesthésiées et ventilées. Le protocole d'imagerie tire profit d'une stratégie d'acquisition et de traitement a posteriori des données permettant de surmonter, sans contrainte mécanique appliquée à l'organe, les problèmes de focalisation liés aux mouvements thoraciques durant la ventilation de l'animal. Cette stratégie originale utilise un sur-échantillonnage de l'acquisition et profite du signal de seconde harmonique généré par le collagène comme référence spatiale ; elle a permis l'observation in vivo d'interactions entre DCs et macrophages au niveau pulmonaire. Ces interactions, de type synapse immunologique, sont favorisées par l'infection et présentent donc un rôle fonctionnel qui reste à définir. La formation de synapses immunologiques entre macrophages et DCs pourrait non seulement représenter un chaînon manquant à l'explication de la pénétration des spores de B. anthracis au niveau pulmonaire, mais pourrait aussi constituer un enjeu crucial dans la compréhension de la réponse immunitaire associée aux infections pulmonaires.Bacillus anthracis, the causative agent of anthrax, is a major bioterrorism pathogen mainly because it can lead to a severe respiratory form of the disease. This form results from inhalation of spores, whose ways of entry into the lungs are not fully understood. This thesis reports the contribution of confocal and two-photon microscopy to the study of the penetration mechanisms of inhaled spores. The animal model utilized was CX3CR1+/gfp mouse, which constitutively expresses the green fluorescent protein (GFP) on CD11b+ dendritic cells (DCs). First, we present an automated method allowing discrimination of DCs among other GFP expressing cells, based on a morphologic coefficient. This method was then applied to the study of the specific behavior of CD11b DCs, after infection by B. anthracis spores. The microscopic study was first performed in situ, i.e. on explanted organs kept in conditions favorable to cell dynamics, then in vivo, i.e. on anesthetized and ventilated mice. In this case the imaging protocol profits from both acquisition and post-processing strategies, and allowed overcoming the focalization pitfalls coming from chest movements during ventilation. This novel strategy is based on an over-sampling of frame acquisition and utilizes second harmonic generation signal from alveolar collagen as a spatial reference. It led to the first ever in vivo observation of interactions between DCs and macrophages at the lung level. These immunological synapse-like structures are promoted by infection and thus display a functional role unknown until now. The formation of macrophages-DCs immunological synapses not only could represent a missing-link in figuring out the B. anthracis spore penetration mechanisms at the lung level, but more importantly could lead to a better understanding of the immune response associated with pulmonary infections.SAVOIE-SCD - Bib.électronique (730659901) / SudocGRENOBLE1/INP-Bib.électronique (384210012) / SudocGRENOBLE2/3-Bib.électronique (384219901) / SudocSudocFranceF

New light on an old problem of the cores of solar resonance lines

We re-examine a 50+ year-old problem of deep central reversals predicted for strong solar spectral lines, in contrast to the smaller reversals seen in observations. We examine data and calculations for the resonance lines of H I, Mg II and Ca II, the self-reversed cores of which form in the upper chromosphere. Based on 3D simulations as well as data for the Mg II lines from IRIS, we argue that the resolution lies not in velocity fields on scales in either of the micro- or macro-turbulent limits. Macro-turbulence is ruled out using observations of optically thin lines formed in the upper chromosphere, and by showing that it would need to have unreasonably special properties to account for critical observations of the Mg II resonance lines from the IRIS mission. The power in turbulence in the upper chromosphere may therefore be substantially lower than earlier analyses have inferred. Instead, in 3D calculations horizontal radiative transfer produces smoother source functions, smoothing out intensity gradients in wavelength and in space. These effects increase in stronger lines. Our work will have consequences for understanding the onset of the transition region, the energy in motions available for heating the corona, and for the interpretation of polarization data in terms of the Hanle effect applied to resonance line profiles.Comment: 12 pages, 9 figures, re-submitted to ap

First High-resolution Spectroscopic Observations of an Erupting Prominence Within a Coronal Mass Ejection by the Interface Region Imaging Spectrograph (IRIS)

Spectroscopic observations of prominence eruptions associated with coronal mass ejections (CMEs), although relatively rare, can provide valuable plasma and 3D geometry diagnostics. We report the first observations by the Interface Region Imaging Spectrograph (IRIS) mission of a spectacular fast CME/prominence eruption associated with an equivalent X1.6 flare on 2014 May 9. The maximum plane-of-sky and Doppler velocities of the eruption are 1200 and 460 km/s, respectively. There are two eruption components separated by ~200 km/s in Doppler velocity: a primary, bright component and a secondary, faint component, suggesting a hollow, rather than solid, cone-shaped distribution of material. The eruption involves a left-handed helical structure undergoing counter-clockwise (viewed top-down) unwinding motion. There is a temporal evolution from upward eruption to downward fallback with less-than-free-fall speeds and decreasing nonthermal line widths. We find a wide range of Mg II k/h line intensity ratios (less than ~2 expected for optically-thin thermal emission): the lowest ever-reported median value of 1.17 found in the fallback material and a comparably high value of 1.63 in nearby coronal rain and intermediate values of 1.53 and 1.41 in the two eruption components. The fallback material exhibits a strong ($> 5 \sigma$) linear correlation between the k/h ratio and the Doppler velocity as well as the line intensity. We demonstrate that Doppler dimming of scattered chromospheric emission by the erupted material can potentially explain such characteristics.Comment: 12 pages, 6 figures, accepted by ApJ (Feb 15, 2015

Two-photon intravital imaging of lungs during anthrax infection reveals long-lasting macrophage-dendritic cell contacts.

International audience: Dynamics of the lung immune system at a microscopic level are largely unknown because of inefficient methods to rid chest motion during image acquisition. In this study, we developed an improved intravital method for two-photon lung imaging uniquely based on a posteriori parenchymal tissue motion correction. We took advantage of the alveolar collagen pattern given by second harmonic generation signal as a reference for frame registration. We describe here for the first time a detailed dynamic account of two major lung immune cell populations, alveolar macrophages and CD11b-positive dendritic cells, during homeostasis and infection by spores of Bacillus anthracis, the agent of anthrax. We show that after alveolar macrophages capture spores, CD11b-positive dendritic cells come in prolonged contact with infected macrophages. Dendritic cells are known to carry spores to the draining lymph nodes and elicit the immune response in pulmonary anthrax. The intimate and long-lasting contacts between these two lines of defense may therefore coordinate immune responses in the lung through an immunological synapse-like process