O uso da citometria de fluxo como critério de cura e aplicabilidade diagnóstica na leishmaniose tegumentar americana (LTA) / The use of flow cytometry as a criteria for healing and diagnostic applicability in american tegumentary leishmaniosis (LTA)

 O objetivo deste trabalho foi avaliar a participação da resposta imune humoral no desenvolvimento de lesões cutâneas e na resposta ao tratamento em indivíduos portadores de LTA através da citometria de fluxo.  Foi realizado um estudo primário, analítico e experimental com ensaios in vitro. Amostras de soros foram inativadas e formas promastigotas de Leishmania (V.) braziliensis foram obtidas a partir da cepa de referência, expandidas em meio Schneider´s até atingir a fase exponencial. Após diversas etapas, uma alíquota de parasitos foi contada e a concentração ajustada para os ensaios de citometria de fluxo. Como resultados da avaliação da aplicabilidade da Imunoglobulina G  em detectar pacientes com LTA ativa, dos 51 pacientes testados, 83,23% foram considerados positivos. Os pacientes foram tratados com a dose 15ml/kg de Glucantime, durante 20 dias, correspondente a um ciclo da medicação. Após 5 meses de tratamento, foi realizado um ensaio com o soro desses pacientes após o tratamento (PT) e foi  comparado com os ensaios realizados antes do tratamento (AT). Não houve diferença significativa em relação aos títulos AT e PT. Por conseguinte, com os resultados obtidos, pode-se sugerir que a IgG é aplicável ao diagnóstico da LTA, uma vez que o teste se mostrou positivo para pacientes com a forma ativa da doença, contribuindo para uma técnica mais específica. Para obter uma diferença significativa entre os títulos AT e PT e determinar um critério de cura laboratorial, é necessário um intervalo de tempo maior para o tratamento, permanecendo como perspectiva continuar o acompanhamento dos pacientes, avaliando-se a redução dos seus títulos.

Synthesis, characterization, antioxidant and antiparasitic activities new naphthyl-thiazole derivatives

Copyright © 2023. Published by Elsevier Inc.In this work, 13 thiosemicarbazones (1a - m) and 16 thiazoles (2a - p) were obtained, which were properly characterized by spectroscopic and spectrometric techniques. The pharmacokinetic properties obtained in silico revealed that the derivatives are in accordance with the parameters established by lipinski and veber, showing that such compounds have good bioavailability or permeability when administered orally. In assays of antioxidant activity, thiosemicarbazones showed moderate to high antioxidant potential when compared to thiazoles. In addition, they were able to interact with albumin and DNA. Screening assays to assess the toxicity of compounds to mammalian cells revealed that thiosemicarbazones were less toxic when compared to thiazoles. In relation to in vitro antiparasitic activity, thiosemicarbazones and thiazoles showed cytotoxic potential against the parasites Leishmania amazonensis and Trypanosoma cruzi. Among the compounds, 1b, 1j and 2l stood out, showing inhibition potential for the amastigote forms of the two parasites. As for the in vitro antimalarial activity, thiosemicarbazones did not inhibit Plasmodium falciparum growth. In contrast, thiazoles promoted growth inhibition. This study shows in a preliminary way that the synthesized compounds have antiparasitic potential in vitro.publishersversionpublishe

SABER TREINAR A SI MESMO: PORQUE E COMO DEVEMOS ENSINAR TREINAMENTO NAS AULAS DE EDUCAÇÃO FÍSICA?

O texto apresenta argumentações cientificas sobre o treino corporal na escola a partir de uma experiência concreta na Escola Municipal Teodoro Sampaio, Salvador, Bahia, Brasil

Vaccines for leishmaniasis and the implications of their development for American tegumentary leishmaniasis

The leishmaniases are a collection of vector-borne parasitic diseases caused by a number of different Leishmania species that are distributed worldwide. Clinical and laboratory research have together revealed several important immune components that control Leishmania infection and indicate the potential of immunization to prevent leishmaniasis. In this review we introduce previous and ongoing experimental research efforts to develop vaccines against Leishmania species. First, second and third generation vaccine strategies that have been proposed to counter cutaneous and visceral leishmaniasis (CL and VL, respectively) are summarized. One of the major bottlenecks in development is the transition from results in animal model studies to humans, and we highlight that although American tegumentary leishmaniasis (ATL; New World CL) can progress to destructive and disfiguring mucosal lesions, most research has been conducted using mouse models and Old World Leishmania species. We conclude that assessment of vaccine candidates in ATL settings therefore appears merited

Bothrops erythromelas snake venom induces a proinflammatory response in mice splenocytes

Submitted by Nuzia Santos ([email protected]) on 2014-11-12T12:16:13Z No. of bitstreams: 1 Bothrops erythromelas snake venom induces a proinflammatory response in mice splenocytes.pdf: 345490 bytes, checksum: f76d0d934776525562cdec7f12fbb45d (MD5)Approved for entry into archive by Nuzia Santos ([email protected]) on 2014-11-12T12:19:19Z (GMT) No. of bitstreams: 1 Bothrops erythromelas snake venom induces a proinflammatory response in mice splenocytes.pdf: 345490 bytes, checksum: f76d0d934776525562cdec7f12fbb45d (MD5)Made available in DSpace on 2014-11-12T12:19:19Z (GMT). No. of bitstreams: 1 Bothrops erythromelas snake venom induces a proinflammatory response in mice splenocytes.pdf: 345490 bytes, checksum: f76d0d934776525562cdec7f12fbb45d (MD5) Previous issue date: 2011Fundação Oswaldo Cruz. Instituto Aggeu Magalhães Departamento de Imunologia. Pernambuco, Brazil/Universidade Estadual da Paraíba. Campina Grande, Brazil;Fundação Oswaldo Cruz. Instituto Aggeu Magalhães Departamento de Imunologia. Pernambuco, Brazil;Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, BrazilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, BrazilFundação Oswaldo Cruz. Instituto Aggeu Magalhães Departamento de Imunologia. Pernambuco, Brazil;Snake venom is a complex biological mixture used for immobilization and killing of prey for alimentation. Many effects are inflicted by this venom, such as coagulation, necrosis, bleeding, inflammation, and shock. This study aimed to evaluate the inflammatory activity promoted by Bothrops erythromelas and Crotalus durissus cascavella snake venom. It was observed that both B. erythromelas and C. d. cascavella venom induced higher interferon-gamma and interleukin-6 production. Nitric oxide (NO) was significantly produced only by B.erythromelas venom, which also showed a higher rate of cell death induction when compared with C. d. cascavella. Results showed that B. erythromelas and C. d. cascavella venom induced distinct response in vitro through cytokines and NO production. However, B. erythromelasinduces a proinflammatory response and a higher rate of cell death in relation to C. d. cascavella venom