QUALITATIVE ASPECTS OF CRIŞUL REPEDE RIVER

Qualitative aspects of Crisul Repede River. The evolution of water quality over the Crişul Repede River is atypical because of natural and anthropic factors. The most significant factors are the geological substrate, and the settlements with their agricultural and industrial activity. The study was performed at three gauging stations for the period 1996-2006, taking into account the annual average values. The considered elements were: the discharge values, temperature, suspensions, oxygen regime, nutrients and phosphorus, taking into consideration their temporal and spatial variation. By comparing them with the admitted limits we could enroll them in various quality classes

MONTHLY AVERAGE FLOW IN RÂUL NEGRU HYDROGRAPHIC BASIN

Râul Negru hydrographic basin represents a well individualised and relatively homogenous physical-geographical unity from Braşov Depression. The flow is controlled by six hydrometric stations placed on the main collector and on two of the most powerful tributaries. Our analysis period is represented by the last 25 years (1988 - 2012) and it’s acceptable for make pertinent conclusions. The maximum discharge month is April, that it’s placed in the high flow period: March – June. Minimum discharges appear in November - because of the lack of pluvial precipitations; in January because of high solid precipitations and because of water volume retention in ice. Extreme discharge frequencies vary according to their position: in the mountain area – small basin surface; into a depression – high basin surface. Variation coefficients point out very similar variation principles, showing a relative homogeneity of flow processes

The variation of several water quality indicators in Red Lake

The natural dam lake from the Hăşmaş Massif is supplied by four permanent streams and various torrential courses. Lake water quality parameters are influenced by natural factors of the region, and the touristic activity of the Red Lake Resort. Evaluation of the water body was done on water samples taken in four sampling campaigns/year. Samples were taken from the middle of the lake, at the water surface. The analysis covers two periods separated by an interval of 11 years: 1993-1999 and 2010-2015. Along with the temporal indicators evolution was analyzed also the standard deviation variation. Deficiencies in respect of employment in quality standards were observed at total phosphorus, total nitrogen, and dissolved oxygen. Regarding total phosphorus, which expresses the degree of eutrophication, the lake water was mesotrophic in the first period, and in the second became oligotrophic. In the future this lacustrine ecosystem requires greater protection to prevent more drastic degradation of water quality

Vertical Dynamics of the Upper Mureș Riverbed

This study navigates the intricate landscape of the Mureș River's upper course, specifically through the contrasting terrains of the Giurgeu Depression and the Toplița-Deda Gorge. It examines the unique relief features and substrates that define the vertical dynamics of the riverbed. The investigation focuses on data collected from four pivotal hydrometric stations: Suseni, Toplița, Stânceni, and Gălăoaia. Here, we determine the thickness of the rock layer (hp) by calculating the difference between the water level and the maximum depth, in relation to the gauge's zero plane. Our analysis highlights the riverbed's monthly regradation patterns within these locales. We uncover notable variations in the scope of vertical dynamics, delineate characteristic periods, and decipher both linear and polynomial trends

Patterns in Mongolian nomadic household movement derived from GPS trajectories

This paper presents an approach for a quantitative analysis of movement patterns of nomadic households based on GPS trajectories. We distributed GPS loggers to 400 Mongolian herder households who carried them over a 9-month period, continuously recording position data every 30min. A total of 142of the resulting trajectories fulfilled our data quality criteria and were considered during the analysis. Based on this data, we derive summary indicators describing key parameters of the households’ mobility including measures of distance and number of movements as well as shape characteristics of the trajectories. We conduct an explorative statistical analysis of these summary indicators to investigate patterns in the nomadic mobility. We identify three movement strategies based on the number of different campsite locations and the distances traveled between campsites. We also compare the results to the existing literature on the mobility of Mongolian herders. Our findings show that GPS-based studies present a suitable framework to quantitatively analyze different movement strategies of nomadic herders

Neuroinflammatory processes are augmented in mice overexpressing human heat-shock protein B1 following ethanol-induced brain injury

Background: Heat-shock protein B1 (HSPB1) is among the most well-known and versatile member of the evolutionarily conserved family of small heat-shock proteins. It has been implicated to serve a neuroprotective role against various neurological disorders via its modulatory activity on inflammation, yet its exact role in neuroinflammation is poorly understood. In order to shed light on the exact mechanism of inflammation modulation by HSPB1, we investigated the effect of HSPB1 on neuroinflammatory processes in an in vivo and in vitro model of acute brain injury. Methods: In this study, we used a transgenic mouse strain overexpressing the human HSPB1 protein. In the in vivo experiments, 7-day-old transgenic and wild-type mice were treated with ethanol. Apoptotic cells were detected using TUNEL assay. The mRNA and protein levels of cytokines and glial cell markers were examined using RT-PCR and immunohistochemistry in the brain. We also established primary neuronal, astrocyte, and microglial cultures which were subjected to cytokine and ethanol treatments. TNF alpha and hHSPB1 levels were measured from the supernates by ELISA, and intracellular hHSPB1 expression was analyzed using fluorescent immunohistochemistry. Results: Following ethanol treatment, the brains of hHSPB1-overexpressing mice showed a significantly higher mRNA level of pro-inflammatory cytokines (Tnf, Il1b), microglia (Cd68, Arg1), and astrocyte (Gfap) markers compared to wild-type brains. Microglial activation, and 1 week later, reactive astrogliosis was higher in certain brain areas of ethanol-treated transgenic mice compared to those of wild-types. Despite the remarkably high expression of pro-apoptotic Tnf, hHSPB1-overexpressing mice did not exhibit higher level of apoptosis. Our data suggest that intracellular hHSPB1, showing the highest level in primary astrocytes, was responsible for the inflammation-regulating effects. Microglia cells were the main source of TNF alpha in our model. Microglia isolated from hHSPB1-overexpressing mice showed a significantly higher release of TNF alpha compared to wild-type cells under inflammatory conditions. Conclusions; Our work provides novel in vivo evidence that hHSPB1 overexpression has a regulating effect on acute neuroinflammation by intensifying the expression of pro-inflammatory cytokines and enhancing glial cell activation, but not increasing neuronal apoptosis. These results suggest that hHSPB1 may play a complex role in the modulation of the ethanol-induced neuroinflammatory response.Peer reviewe

Cultured cells of the blood-brain barrier from apolipoprotein B-100 transgenic mice: effects of oxidized low-density lipoprotein treatment

BACKGROUND: The apolipoprotein B-100 (ApoB-100) transgenic mouse line is a model of human atherosclerosis. Latest findings suggest the importance of ApoB-100 in the development of neurodegenerative diseases and microvascular/perivascular localization of ApoB-100 protein was demonstrated in the cerebral cortex of ApoB-100 transgenic mice. The aim of the study was to characterize cultured brain endothelial cells, pericytes and glial cells from wild-type and ApoB-100 transgenic mice and to study the effect of oxidized low-density lipoprotein (oxLDL) on these cells. METHODS: Morphology of cells isolated from brains of wild type and ApoB-100 transgenic mice was characterized by immunohistochemistry and the intensity of immunolabeling was quantified by image analysis. Toxicity of oxLDL treatment was monitored by real-time impedance measurement and lactate dehydrogenase release. Reactive oxygen species and nitric oxide production, barrier permeability in triple co-culture blood–brain barrier model and membrane fluidity were also determined after low-density lipoprotein (LDL) or oxLDL treatment. RESULTS: The presence of ApoB-100 was confirmed in brain endothelial cells, while no morphological change was observed between wild type and transgenic cells. Oxidized but not native LDL exerted dose-dependent toxicity in all three cell types, induced barrier dysfunction and increased reactive oxygen species (ROS) production in both genotypes. A partial protection from oxLDL toxicity was seen in brain endothelial and glial cells from ApoB-100 transgenic mice. Increased membrane rigidity was measured in brain endothelial cells from ApoB-100 transgenic mice and in LDL or oxLDL treated wild type cells. CONCLUSION: The morphological and functional properties of cultured brain endothelial cells, pericytes and glial cells from ApoB-100 transgenic mice were characterized and compared to wild type cells for the first time. The membrane fluidity changes in ApoB-100 transgenic cells related to brain microvasculature indicate alterations in lipid composition which may be linked to the partial protection against oxLDL toxicity. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12987-015-0013-y) contains supplementary material, which is available to authorized users

Cerebrovascular Pathology in Hypertriglyceridemic APOB-100 Transgenic Mice

Hypertriglyceridemia is not only a serious risk factor in the development of cardiovascular diseases, but it is linked to neurodegeneration, too. Previously, we generated transgenic mice overexpressing the human APOB-100 protein, a mouse model of human atherosclerosis. In this model we observed high plasma levels of triglycerides, oxidative stress, tau hyperphosphorylation, synaptic dysfunction, cognitive impairment, increased neural apoptosis and neurodegeneration. Neurovascular dysfunction is recognized as a key factor in the development of neurodegenerative diseases, but the cellular and molecular events linking cerebrovascular pathology and neurodegeneration are not fully understood. Our aim was to study cerebrovascular changes in APOB-100 transgenic mice. We described the kinetics of the development of chronic hypertriglyceridemia in the transgenic animals. Increased blood-brain barrier permeability was found in the hippocampus of APOB-100 transgenic mice which was accompanied by structural changes. Using transmission electron microscopy, we detected changes in the brain capillary endothelial tight junction structure and edematous swelling of astrocyte endfeet. In brain microvessels isolated from APOB-100 transgenic animals increased Lox-1, Aqp4, and decreased Meox-2, Mfsd2a, Abcb1a, Lrp2, Glut-1, Nos2, Nos3, Vim, and in transgenic brains reduced Cdh2 and Gfap-σ gene expressions were measured using quantitative real-time PCR. We confirmed the decreased P-glycoprotein (ABCB1) and vimentin expression related to the neurovascular unit by immunostaining in transgenic brain sections using confocal microscopy. We conclude that in chronic hypertriglyceridemic APOB-100 transgenic mice both functional and morphological cerebrovascular pathology can be observed, and this animal model could be a useful tool to study the link between cerebrovascular pathology and neurodegeneration

Examining the Relationship Between Different Retail Mediums and Consumer Spending Habits

Understanding customer behavior and preferences is the first step towards developing optimal strategies for businesses and driving the highest revenues possible. Consumer behavior spending habits are everchanging due to the evolving nature of customers’ needs and preferences. This thesis fills the gap of a quantitative analysis between the in-person and online retail medium reflected onto levels of customer spending. This research provides further information about shoppers’ decision-making processes accounting for correlative relationships between demographic (age, gender, education level, income, etc.) and environmental variables (physical store atmospheres, online retail websites, social media advertisement). The research was based on primary data collected via survey, a timely and reflective format for observing current customer spending habits and behaviors in both in-person and online environments. The sample population was primarily constituted of Northeastern Illinois University students, faculty, and staff, along with other non-affiliated participants outside the institution in order to increase diversity of responses and reflectivity of the overall consumer population. The survey collected quantitative data along with indicator, dummy, and dichotomous variables based on Likert-scaled responses. Multiple linear regression analyses were implemented to identify correlative relationships between independent (demographic and environmental variables) and dependent variables (monetary spending) to highlight their contribution over levels of spending