GABA-enhanced collective behavior in neuronal axons underlies persistent gamma-frequency oscillations

Gamma (30–80 Hz) oscillations occur in mammalian electroencephalogram in a manner that indicates cognitive relevance. In vitro models of gamma oscillations demonstrate two forms of oscillation: one occurring transiently and driven by discrete afferent input and the second occurring persistently in response to activation of excitatory metabotropic receptors. The mechanism underlying persistent gamma oscillations has been suggested to involve gap-junctional communication between axons of principal neurons, but the precise relationship between this neuronal activity and the gamma oscillation has remained elusive. Here we demonstrate that gamma oscillations coexist with high-frequency oscillations (>90 Hz). High-frequency oscillations can be generated in the axonal plexus even when it is physically isolated from pyramidal cell bodies. They were enhanced in networks by nonsomatic -aminobutyric acid type A (GABAA) receptor activation, were modulated by perisomatic GABAA receptor-mediated synaptic input to principal cells, and provided the phasic input to interneurons required to generate persistent gamma-frequency oscillations. The data suggest that high-frequency oscillations occurred as a consequence of random activity within the axonal plexus. Interneurons provide a mechanism by which this random activity is both amplified and organized into a coherent network rhythm

Spectral Evolution of an Earth-Like Planet

We have developed a characterization of the geological evolution of the Earths atmosphere and surface in order to model the observable spectra of an Earth-like planet through its geological history. These calculations are designed to guide the interpretation of an observed spectrum of such a planet by future instruments that will characterize exoplanets. Our models focus on spectral features that either imply habitability or are required for habitability. These features are generated by H2O, CO2, CH4, O2, O3, N2O, and vegetation-like surface albedos. We chose six geological epochs to characterize. These epochs exhibit a wide range in abundance for these molecules, ranging from a CO2 rich early atmosphere, to a CO2/CH4-rich atmosphere around 2 billion years ago to a present-day atmosphere. We analyzed the spectra to quantify the strength of each important spectral feature in both the visible and thermal infrared spectral regions, and the resolutions required to unambiguously observe the features for each epoch. We find a wide range of spectral resolutions required for observing the different features. For example, H2O and O3 can be observed with relatively low resolution, while O2 and N2O require higher resolution. We also find that the inclusion of clouds in our models significantly affects both the strengths and resolutions required to observe all spectral features.Comment: 34 pages, 24 fig, pdf, ApJ, TB

Conditional sampling for barrier option pricing under the LT method

We develop a conditional sampling scheme for pricing knock-out barrier options under the Linear Transformations (LT) algorithm from Imai and Tan (2006). We compare our new method to an existing conditional Monte Carlo scheme from Glasserman and Staum (2001), and show that a substantial variance reduction is achieved. We extend the method to allow pricing knock-in barrier options and introduce a root-finding method to obtain a further variance reduction. The effectiveness of the new method is supported by numerical results

Tractability of multivariate problems for standard and linear information in the worst case setting: part II

We study QPT (quasi-polynomial tractability) in the worst case setting for linear tensor product problems defined over Hilbert spaces. We assume that the domain space is a reproducing kernel Hilbert space so that function values are well defined. We prove QPT for algorithms that use only function values under the three assumptions: 1) the minimal errors for the univariate case decay polynomially fast to zero, 2) the largest singular value for the univariate case is simple and 3) the eigenfunction corresponding to the largest singular value is a multiple of the function value at some point. The first two assumptions are necessary for QPT. The third assumption is necessary for QPT for some Hilbert spaces

Clinical investigations concerning the use of Ethrane for Cesarean section

Peer Reviewe

Expression of connexins in human preimplantation embryos in vitro

Intercellular communication via gap junctions is required to coordinate developmental processes in the mammalian embryo. We have investigated if the connexin (Cx) isoforms known to form gap junctions in rodent preimplantation embryos are also expressed in human embryos, with the aim of identifying species differences in communication patterns in early development. Using a combination of polyA PCR and immunocytochemistry we have assessed the expression of Cx26, Cx31, Cx32, Cx40, Cx43 and Cx45 which are thought to be important in early rodent embryos. The results demonstrate that Cx31 and Cx43 are the main connexin isoforms expressed in human preimplantation embryos and that these isoforms are co-expressed in the blastocyst. Cx45 protein is expressed in the blastocyst but the protein may be translated from a generally low level of transcripts: which could only be detected in the PN to 4-cell embryos. Interestingly, Cx40, which is expressed by the extravillous trophoblast in the early human placenta, was not found to be expressed in the blastocyst trophectoderm from which this tissue develops. All of the connexin isoforms in human preimplantation embryos are also found in rodents pointing to a common regulation of these connexins in development of rodent and human early embryos and perhaps other species

A role for fast rhythmic bursting neurons in cortical gamma oscillations in vitro

Basic cellular and network mechanisms underlying gamma frequency oscillations (30–80 Hz) have been well characterized in the hippocampus and associated structures. In these regions, gamma rhythms are seen as an emergent property of networks of principal cells and fast-spiking interneurons. In contrast, in the neocortex a number of elegant studies have shown that specific types of principal neuron exist that are capable of generating powerful gamma frequency outputs on the basis of their intrinsic conductances alone. These fast rhythmic bursting (FRB) neurons (sometimes referred to as "chattering" cells) are activated by sensory stimuli and generate multiple action potentials per gamma period. Here, we demonstrate that FRB neurons may function by providing a large-scale input to an axon plexus consisting of gap-junctionally connected axons from both FRB neurons and their anatomically similar counterparts regular spiking neurons. The resulting network gamma oscillation shares all of the properties of gamma oscillations generated in the hippocampus but with the additional critical dependence on multiple spiking in FRB cells

A Survey of University Policy Makers' Preferences and Expectations for Provincial Examinations

A survey was conducted of persons responsible for making undergraduate admission policies at Ontario universities to ascertain their preferences and expectations for provincial examinations. Fifty-eight individuals, at least two from each university, responded to a series of questions by telephone interview or questionnaire. Strong support was expressed for the reintroduction of provincial examinations for mathematics and first language (English or français) courses in the final year of the secondary school program. Most respondents rejected the use of scores on province-wide examinations for rating secondary schools and adjusting school marks. Instead, they expressed a preference for having applicants report both teacher-assigned course marks and provincial examination scores.Nous avons mené une enquête auprès des personnes responsables des règlements d'admission au premier cycle dans les universités de l'Ontario afin de connaître leurs attentes et préférences au sujet des examens provinciaux. Cinquante-huit personnes, dont au moins deux de chaque université, ont répondu à une série de questions par voie téléphonique ou par questionnaire. Les répondants ont montré une forte tendance en faveur de la ré-introduction d'examens provinciaux pour les mathématiques et la langue première (l'anglais ou le français), examens qui seraient administrés à la fin de la dernière année d'école secondaire. La plupart on rejeté l'idée de se servir des résultats de ces examens pour l'évaluation des écoles secondaires et pour le réajustement des notes scolaires. En fait, ils préféreraient que les candidats présentent les notes accordées par leurs enseignants ainsi que les résultats de leurs examens