Short duration reservoir-release impacts on impounded upland rivers

The increasing number and scale of river impoundments throughout the 19th and 20th centuries means that the management of these impoundments is crucial to the future of global riverine biota. Impoundments such as reservoirs can affect rivers in a variety of ways, not least through the reduction in amplitude of the natural hydrograph, depriving rivers of ecologically important spate flows. Many reservoir operators conduct regular safety tests, known as scour releases, during which large quantities of impounded water are released directly into rivers. This project assesses the impact of these releases on the hydrology and physio-chemistry of the receiving water bodies as well as upon fish movements and benthic macroinvertebrate abundance and diversity downstream of the reservoirs. The potential of such releases to mimic natural spate flows for ecological gain is also examined. The work took place in the Yorkshire Water catchment area in northern England between 2007 and 2010. Passive Integrated Transponder (PIT) telemetry was used to assess the responses of brown trout Salmo trutta to these short-duration releases. Tagged fish were able to maintain position during the releases and showed no evidence of wash-out or upstream migratory movements associated with the releases. Changes to macroinvertebrate abundance, diversity and community structure associated with the release were also examined. Some sites showed significant wash-out and community change following the releases while other sites were unchanged. Communities at impacted sites returned to pre-release structures within weeks of the releases. Analysis of habitat use and characteristics suggest the responses of fish and macroinvertebrates to these reservoir releases were linked to habitat heterogeneity and the use of flow refugia. The negative impacts associated with the scour releases were minimal, while mimicked spate releases may improve salmonid spawning habitat and could re-introduce valuable flow variability to impounded catchments

Somites and Axon Guidance

The somites are arrayed in a repeating pattern along the longitudinal axis of the embryo, as are the developing sensory and sympathetic ganglia and the spinal nerves. This pattern is not a coincidence: the somite imposes a segmental pattern on the cells and axons that invade it. Both neural crest cells and axons prefer the anterior portion of the sclerotome (the ventral part of the somite) for outgrowth. What differences in anterior and posterior sclerotome are responsible? I used scanning electron microscopy to ask whether these populations differed on the tissue level in chick embryos. This study shows that differences in tissue organization are of insufficient magnitude or develop too late to explain the preference of neural crest cells and axons for the anterior half of each sclerotome. For instance, the extracellular matrix does not differ dramatically in density at the dorsal sclerotome boundary and yet neural crest cells promptly enter the anterior sclerotome when they reach this boundary. These cells have access to the cell processes of somitic cells that extend through the matrix. This suggests that neural crest cells could detect important differences in anterior and posterior populations by direct cell contact. Likewise, barriers and consistent differences in cell density, shape or orientation were not obvious before or during initial axon outgrowth. The absence of significant differences in tissue organization suggests that axons and neural crest cells become segmented by responding to diffusible cues, to differences in extracellular material or to the cell surfaces of individual anterior and posterior sclerotome cells

Gaming in Britain and America: Some Historical Comparisons

This paper compares the development of gambling in Britain during the late 17th and 18th centuries with the emergence of gambling in Nevada during the late 19th and early 20th centuries. Drawing on the existence of similar themes and ideas in different contexts, the author demonstrates several benefits of comparative studies of gambling. Focusing principally on gambling games played with cards and dice, this paper begins by examining approaches to taxing gaming before moving on to consider regulatory strategies

Commercialization, Crime, and Casinos: Legacies of 18th Century Gambling

This talk features Dr. Tosney, who recently received his Ph.D. in history from the University of York and who served as the Center\u27s April visiting fellow. He speaks about the development of licensing, regulation, and moral attitudes concerning gambling in both early modern England and 20th century Las Vegas

Cell death delineates axon pathways in the hindlimb and does so independently of neurite outgrowth

We wished to know whether the cell death and phagocytosis seen near the outgrowing nerve front in the hindlimb delineate axon pathways and, if so, whether the cells died only in the presence of growth cones. We unilaterally deleted the lumbosacral neural tube and reconstructed the patterns of neurite outgrowth and phagocytes during the stage when neurites first begin to colonize the thigh. In the control limbs, sensory and motor nerve pathways coincided with sites of phagocytosis, including those pathways that had yet to be colonized by growth cones. For instance, phagocytes were clustered at foci within the muscle masses where muscle nerves form a day later. However, they were not seen in adjacent, nonpathway regions such as posterior sclerotome or dorsal and ventral to the region of the plexus in which axons extend only posteriorly. Phagocytes were also seen in defined regions that are probably inaccessible to growth cones because they are too distant from pathways (i.e., subjacent to the apical ectodermal ridge) or express substances that are typical of precartilagenous tissues which may prohibit axon advance. In the experimental limbs, we conservatively estimated that neurite outgrowth was reduced to less than one-tenth (neurites were visible only with electron microscopy) or less than one-third of normal. Outgrowth extended less far distally and, in half the cases, motor innervation was completely abolished. Despite the extensive reduction in neurite outgrowth, the distribution of phagocytes was indistinguishable from that of the control side. Furthermore, the number of phagocytes did not differ significantly. We conclude that cell death delineates axon pathways remarkably well and does so without an interaction with growth cones; it is an independent characteristic of the axonal pathways and may be directly or indirectly important to axonal pathfinding. This is the first identification of a feature that characterizes prospective nerve pathways in the hindlimb.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/27050/1/0000040.pd

Cellular Interactions That Guide Sensory and Motor Neurites Identified in an Embryo Slice Preparation

AbstractWe used cultured cross sections (“slices”) of avian embryos to identify interactions that guide neurites during their encounters with seven tissues that impose a stereotyped gross anatomical nerve pattern. We show that cultured slices retain tissue morphology, molecular distribution patterns, and guidance cues. They also allow us to directly visualize responses of labeled sensory and motor neurons deposited on the slice's surface. This assay has high predictive power. Contact-mediated avoidance or stimulation and long-range attraction or repulsion are each distinguishable because each predicts different neurite lengths and trajectories. The analysis shows that all but one of these mechanisms contributes to guidance. Three tissues similarly stimulated neurite elongation, suggesting common responses to a contact-mediated stimulation. Four tissues similarly elicited avoidance on contact, suggesting a common contact-mediated inhibition. Neurite orientations implicate a previously unsuspected long-distance attraction to one tissue, dorsal anterior sclerotome. Long-range repulsion plays no detectable role. Each tissue elicits the same response in two different neural populations, sensory and motor neurons. These results suggest that a small set of repeated mechanisms mediates responses to tissues that axons contact serially during pathfinding

Distribution and projection pattern of motoneurons that innervate hindlimb muscles in the quail

We characterized the motoneuron pool positions and projection patterns in the embryonic quail hindlimb and compared them to those in the chick to determine the degree of similarity and to form a baseline for future chimeric experiments. We find that the most similar parameters of pool position correlate with the major axonal pathway choices. First, the medial-lateral pool position, which is highly conserved among birds and mammals, is identical in the quail and chick and correlates with the dorsal-ventral pathway choice, the first and least plastic of the choices within the limb. Second, although quail pools were known to be compressed into seven rather than eight segments, we show that the map of pools is compressed about a central point (segment three) that preserves the spatial relationships between anterior pools and the crural plexus, and between posterior pools and the sciatic plexus. Access to guidance cues that are restricted to each plexus region is thus maintained between species. Third, pool position along the anterior-posterior axis is the least similar parameter between species. In fact, the entire lumbosacral motor complex may shift by ± half a segment in individual quail. Despite the consequent differences in segmental projections, the specific projection pattern within each quail hindlimb is identical to that in the chick. There is no need to preserve the exact segmental pattern either phylogenetically or during development, because motoneurons accommodate to modest variations in their position along the anterior-posterior axis by sorting out at the limb base. The contrast between variable segmental and constant limb projections also demonstrates that neither the specification nor the precise projection of motoneurons is dependent upon the specification or development of somites.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/50047/1/902980404_ftp.pd

Proximal tissues and patterned neurite outgrowth at the lumbosacral level of the chick embryo: Deletion of the dermamyotome

The role of the dermamyotome (the dorsal portion of the somite which gives rise to muscles and dermis) in the development of patterned axon outgrowth was examined under conditions where limb development was substantially undisturbed. One or more chick dermamyotomes were removed before or during early neurite outgrowth and subsequent development was examined. Several developmental processes suspected to depend on the dermamyotome were not altered by its removal: (1) Neural crest cells that form sensory ganglia migrated and condensed in their normal segmental pattern. (2) The distal progression, dorsal-ventral organization, and segmentation of spinal nerves were unaltered. (3) Motoneuron pathway selection and projection patterns in the limb were normal in all respects.The most interesting finding was that the formation of the dorsal ramus is dependent on the nearby dermamyotome which provides the targets for this nerve. When a single or two adjacent dermamyotomes were removed, the metameric epaxial muscles derived from each dermamyotome were absent and the dorsal ramus extended into epaxial muscle in the closest adjacent segment. However, when dermamyotomes in both adjacent segments had also been removed or substantially reduced, the dorsal ramus did not form. These results strongly suggest that the target provides a chemotactic signal for proper outgrowth of dorsal ramus axons.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/26630/1/0000171.pd

Assessing the environmental and economic efficacy of two integrated constructed wetlands at mitigating eutrophication risk from sewage effluent

The nutrient removal efficiency of two integrated constructed wetlands (ICWs) installed at commercial wastewater treatment plants (WWTPs) in Norfolk, UK, is assessed – the River Ingol ICW (1 year old) and the River Mun ICW (5 years old). Analysing water samples collected across the ICWs between February and September 2019, significant reductions in both effluent nutrient concentration and load were recorded. At the River Mun ICW, mean nitrate and phosphate concentrations were reduced by ~63% across the wetland, whilst nutrient loadings were reduced by ~57%. At the River Ingol ICW, mean nitrate and phosphate concentrations were reduced by ~30%, whilst nutrient loadings were reduced by ~70%. Economically, the total capital cost of both ICWs was comparable at £31-39 per person served. Overall, this study demonstrates ICWs can significantly reduce the eutrophication risk associated with WWTP discharges and can do so whilst providing a cost-effective alternative to conventional tertiary wastewater treatment

Proximal tissues and patterned neurite outgrowth at the lumbosacral level of the chick embryo: Partial and complete deletion of the somite

The development of patterned axon outgrowth and dorsal root ganglion (DRG) formation was examined after partially or totally removing chick somitic mesoderm. Since the dermamyotome is not essential and a full complement of limb muscles developed, alterations in neural patterns could be ascribed to deletion of sclerotome. When somitic tissue was completely removed, axons extended and DRG formed, but in an unsegmented pattern. Therefore the somite does not elicit outgrowth of axons or migration of DRG precursors, it is not a manditory substratum and it is not required for DRG condensation. These results suggest that posterior sclerotome is relatively inhibitory to invasion, an inhibition that is released when sclerotome is absent. When somites were partially deleted, axonal segmentation was not lost proportionally with the amount of sclerotome removed, suggesting that properties that may vary with sclerotome volume (such as diffusible cues) do not play a primary role. Instead, spinal nerves lost segmentation only when ventral sclerotome was deleted, regardless of whether dorsal sclerotome was or was not removed. This strongly suggests that axonal segmentation is imposed by direct interactions between growth cones and extracellular matrices or surfaces of sclerotome cells. While DRG tended to be normally segmented when ventral sclerotome was deleted and to lose segmentation when dorsomedial sclerotome was absent, a coordinate loss of DRG segmentation with sclerotome volume could not be ruled out. However it is clear that axonal and DRG segmentation are independent. Observations on a subset of embryos in which the notochord was displaced relative to the spinal cord suggest that the ventromedial sclerotome surrounding the notochord inhibits axon advance. Posterior and ventromedial sclerotome are hypothesized to act as barriers to axon outgrowth due to some feature of their common cartilaginous development. Specific innervation patterns were also examined. When the notochord was displaced toward the control limb, axons on this side made and corrected projection errors, suggesting that the notochord can influence the precision of axonal pathway selection. In contrast, motor axons that entered the limb on all operated sides innervated muscle with their normal precision despite the absence of the somite and axonal segmentation. Therefore, the somite and the process of spinal nerve segmentation are largely irrelevant to the specificity of motoneuron projection.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/27277/1/0000293.pd