Rapid identification of dragon blood samples from Daemonorops draco, Dracaena cinnabari and Dracaena cochinchinensis by MALDI‐TOF mass spectrometry

[[abstract]]Introduction Dragon blood is a deep‐red plant resin which has been used as folk medicine for more than a thousand years. It can be produced from at least four entirely different plant families: Asparagaceae, Arecaceae, Chamaesyce, and Fabaceae. Current pharmacopeia states that the only “authentic” source of dragon blood is the palm tree, Daemonorops draco . Objective The present study aims to find a high‐throughput method to screen and identify the plant sources of commercial dragon blood products. Methodology A matrix‐assisted laser desorption ionisation time‐of‐flight mass spectrometry (MALDI‐TOF MS) based method for rapid screening of dracorhodin in commercial dragon blood samples was established in this study. Results Well‐resolved peaks of dracorhodin in spectra were observed in the crude extracts of samples. Dragon blood samples from two other plant species, Dracaena cinnabari and Dracaena cochinchinensis , were also examined. Their indicator compounds, loureirin A and B, were detected in these plants. Conclusion A MALDI‐TOF based method for preliminarily examination of commercial dragon blood samples is reported here. In contrast to MALDI‐TOF, liquid chromatography mass spectrometry (LC‐MS) is a time‐consuming and costly method, not ideal for routine and large‐scale screening of commercial samples.[[notice]]補正完

Quality evaluation of various commercial specifications of Polygoni Multiflori Radix and its dregs by determination of active compounds

<p>Abstract</p> <p>Background</p> <p>According to market investigation, two kinds of Polygoni Multiflori Radix decoction pieces with different specifications are commercially available: irregular thick slices (0.7-1.3 cm) and length-wise into thin slices (0.11-0.2 cm). The objective of this study was to evaluate the quality of various samples of Polygoni Multiflori Radix decoction pieces and its dregs.</p> <p>Results</p> <p>A simple and reliable high performance liquid chromatographic method was developed for determination the contents of 2,3,5,4′-tetrahydroxystilbene-2-<it>O</it>-<it>β</it>-<it>D</it>- glucopyranoside (THSG), emodin and physcion, which were considered to be potent active ingredients. The results showed that the contents of THSG, emodin and physcion varied in samples of different diameters and thicknesses. The results also indicated the dregs of Polygoni Multiflori Radix still contained a considerable amount of THSG, emodin and physcion.</p> <p>Conclusion</p> <p>The various commercial specifications of Polygoni Multiflori Radix sold in the markets did not correlate with their prices, and the dregs of Polygoni Multiflori Radix can be further utilized.</p