Способы функционализации титановых имплантатов наночастицами серебра

Работа посвящена поиску оптимального метода функционализации металлическихтитановых подложек наночастицами серебра для формирования антибактериального интерфейса на поверхности экспериментальных образцов

Deposition of Ultrathin Nano-Hydroxyapatite Films on Laser Micro-Textured Titanium Surfaces to Prepare a Multiscale Surface Topography for Improved Surface Wettability/Energy

The primary aim of this study was to analyse the correlation between topographical features and chemical composition with the changes in wettability and the surface free energy of microstructured titanium (Ti) surfaces. Periodic microscale structures on the surface of Ti substrates were fabricated via direct laser interference patterning (DLIP). Radio-frequency magnetron sputter deposition of ultrathin nanostructured hydroxyapatite (HA) films was used to form an additional nanoscale grain morphology on the microscale-structured Ti surfaces to generate multiscale surface structures. The surface characteristics were evaluated using atomic force microscopy and contact angle and surface free energy measurements. The structure and phase composition of the HA films were investigated using X-ray diffraction. The HA-coated periodic microscale structured Ti substrates exhibited a significantly lower water contact angle and a larger surface free energy compared with the uncoated Ti substrates. Control over the wettability and surface free energy was achieved using Ti substrates structured via the DLIP technique followed by the deposition of a nanostructured HA coating, which resulted in the changes in surface chemistry and the formation of multiscale surface topography on the nano- and microscale

Influence of Anodization Time and Voltage on the Parameters of TiO[2] Nanotubes

A vertically aligned titania nanotube layer was obtained by electrochemical anodic oxidation in the electrolyte contained 0.4 wt% solution of NH[4]F in 54 ml of ethylene glycol and 5 ml of deionized water, after titanium was chemically cleaned/etched with a mixture of HCl, H[2]O and HNO[3] solution for removing the natural oxide films. The morphology and composition of the titania nanotube layer were examined by scanning electron microscopy (SEM) and energy-dispersive X-ray spectroscopy (EDS). The anodization of TiO[2] nanotubes was done using 60 V for 240 min and 30 min, and 30 V for 30 min. The diameter of the titania nanotubes was about 52-156 nm, the wall thickness about 32-53 nm and the height about 0.9-6.3 [mu]m. The pore size of TiO[2] nanotubes influences the dissolution rate of CaP thin films and Young's modulus, which is significantly lower than that of the Ti substrate. Our future challenge will be investigation of the microstructure and mechanical behavior of titania nanotubes with CaP film

Effect of the SR-containing hydroxyapatite nanoparticles doping on the polymer fiber morphology within the 3-D artificial scaffolds for bone tissue regeneration

Functionalized 3-D scaffolds based on polycaprolactone (PCL) with strontium-containing hydroxyapatite (Sr-HA) were prepared via electrospinning technique. Scanning electron microscopy (SEM) and Fourier transform infrared spectroscopy (FTIR) were used to investigate the structure and morphology of the scaffolds. The experimental results revealed that due to incorporation of Sr-HA particles into the polymer fibers, the surface of PCL/Sr-HA hybrid 3-D polymer scaffolds possessed porous and rough structure, which potentially should provide stimulation of adhesion and growth of bone cells

Radio Frequency Magnetron Sputter Deposition as a Tool for Surface Modification of Medical Implants

The resent advances in radio frequency (RF)‐magnetron sputtering of hydroxyapatite films are reviewed and challenges posed. The principles underlying RF‐magnetron sputtering used to prepare calcium phosphate‐based, mainly hydroxyapatite coatings, are discussed in this chapter. The fundamental characteristic of the RF‐magnetron sputtering is an energy input into the growing film. In order to tailor the film properties, one has to adjust the energy input into the substrate depending on the desired film properties. The effect of different deposition control parameters, such as deposition time, substrate temperature, and substrate biasing on the hydroxyapatite (HA) film properties is discussed

Synthesis of positively and negatively charged silver nanoparticles and their deposition on the surface of titanium

Bacterial infections related to dental implants are currently a significant complication. A good way to overcome this challenge is functionalization of implant surface with Ag nanoparticles (NPs) as antibacterial agent. This article aims at review the synthesis routes, size and electrical properties of AgNPs. Polyvinyl pyrrolidone (PVP) and polyethyleneimine (PEI) were used as stabilizers. Dynamic Light Scattering, Nanoparticle Tracking Analysis, X-ray diffraction (XRD), scanning electron microscopy (SEM), and energy dispersive spectroscopy (EDX) have been used to characterize the prepared AgNPs. Two types of NPs were synthesized in aqueous solutions: PVP-stabilized NPs with a diameter of the metallic core of 70 ± 20 nm, and negative charge of -20 mV, PEI-stabilized NPs with the size of the metallic core of 50 ± 20 nm and positive charge of +55 mV. According to SEM results, all the NPs have a spherical shape. Functionalization of the titanium substrate surface with PVP and PEI-stabilized AgNPs was carried out by dropping method. XRD patterns revealed that the AgNPs are crystalline with the crystallite size of 14 nm

GPU-accelerated ray-casting for 3D fiber orientation analysis

Orientation analysis of fibers is widely applied in the fields of medical, material and life sciences. The orientation information allows predicting properties and behavior of materials to validate and guide a fabrication process of materials with controlled fiber orientation. Meanwhile, development of detector systems for high-resolution non-invasive 3D imaging techniques led to a significant increase in the amount of generated data per a sample up to dozens of gigabytes. Though plenty of 3D orientation estimation algorithms were developed in recent years, neither of them can process large datasets in a reasonable amount of time. This fact complicates the further analysis and makes impossible fast feedback to adjust fabrication parameters. In this work, we present a new method for quantifying the 3D orientation of fibers. The GPU implementation of the proposed method surpasses another popular method for 3D orientation analysis regarding accuracy and speed. The validation of both methods was performed on a synthetic dataset with varying parameters of fibers. Moreover, the proposed method was applied to perform orientation analysis of scaffolds with different fibrous micro-architecture studied with the synchrotron μCT imaging setup. Each acquired dataset of size 600x600x450 voxels was analyzed in less 2 minutes using standard PC equipped with a single GPU

Hybrid piezoelectric and biodegradable polymer-based scaffolds for biomedical applications

The authors thanks to Mr. A. Anyugin and A. Zviagin for the assistance with experiments. The authors acknowledge the financial support from the Russian Science Foundation (project #18-73-10050)

Development of multilayer Hydroxyapatite - Ag/TiN-Ti coatings deposited by radio frequency magnetron sputtering with potential application in the biomedical field

"NOTICE: this is the author's version of a work that was accepted for publication in Surface and Coatings Technology. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Surface and Coatings Technology, VOL 377, (2019) DOI 10.1016/j.surfcoat.2019.06.097"[EN] The use of composite coatings is emerging as a great alternative to conventional coatings, allowing the combination of different superficial properties that are widely desired in surgical implants, such as osteointegration and bactericidal character, and cannot be provided by one material alone. In the present investigation the effect of the incorporation of a TiN-Ti intermediate bilayer on the chemical composition, structure, morphology, roughness, residual stresses and adhesion of a multi-layer Hydroxyapatite (HA)-Ag coating deposited on Ti-6Al-4V by magnetron sputtering was evaluated. Additionally, the cytotoxicity of the developed system was evaluated by in vitro tests. According to the results obtained, a decrease in the Ca/P ratio from 1.85 to 1.74 was obtained through the deposition of an HA-Ag system on the intermediate bilayer, and the crystallinity of the developed coating was favored. The multi-layer structure was effectively observed by field emission scanning electron microscopy, where it was possible to identify each of the HA, Ag, TiN and Ti layers. Meanwhile, an increase of 7% in crystallite size, a decrease of 36% in residual stresses and an increase of 32% in adhesion were registered for this composite coating compared to the free intermediate bilayer system. Finally, biological evaluation allowed the non-cytotoxic character of the deposited coatings to be confirmed.We thank the University of Antioquia, the Centro de Investigation, Innovation y Desarrollo de materiales (CIDEMAT) group, the Departamento Administrativo de Ciencia, Tecnologia e Innovation (COLCIENCIAS) for financing the Project 15-1696, the scholarship program of Enlazamundos, PR and JLGR acknowledge financial support from the Spanish Ministry of Economy and Competitiveness (MINECO) through the project MAT2016-76039-C4-1-R (AEI/FEDER, UE) (including the FEDER financial support). CIBER-BBN is an initiative funded by the VI National R&D&I Plan 2008-2011, Iniciativa Ingenio 2010, Consolider Program. CIBER Actions are financed by the Institute de Salud Carlos III with assistance from the European Regional Development Fund.Lenis, J.; Bejarano, G.; Rico Tortosa, PM.; Gómez Ribelles, JL.; Bolívar, F. (2019). Development of multilayer Hydroxyapatite - Ag/TiN-Ti coatings deposited by radio frequency magnetron sputtering with potential application in the biomedical field. Surface and Coatings Technology. 377:1-9. https://doi.org/10.1016/j.surfcoat.2019.06.097S19377Melero, H., Fernández, J., & Guilemany Casadamon, J. M. (2011). Recubrimientos bioactivos: Hidroxiapatita y titania. Biomecánica, 19(1). doi:10.5821/sibb.v19i1.1814Ozeki, K., Yuhta, T., Fukui, Y., & Aoki, H. (2002). Phase composition of sputtered films from a hydroxyapatite target. Surface and Coatings Technology, 160(1), 54-61. doi:10.1016/s0257-8972(02)00363-8Nelea, V., Morosanu, C., Iliescu, M., & Mihailescu, I. N. (2003). Microstructure and mechanical properties of hydroxyapatite thin films grown by RF magnetron sputtering. Surface and Coatings Technology, 173(2-3), 315-322. doi:10.1016/s0257-8972(03)00729-1Mohseni, E., Zalnezhad, E., Bushroa, A. R., Abdel Magid Hamouda, Goh, B. T., & Yoon, G. H. (2015). Ti/TiN/HA coating on Ti–6Al–4V for biomedical applications. Ceramics International, 41(10), 14447-14457. doi:10.1016/j.ceramint.2015.07.081Nelea, V., Morosanu, C., Bercu, M., & Mihailescu, I. N. (2007). Interfacial titanium oxide between hydroxyapatite and TiAlFe substrate. Journal of Materials Science: Materials in Medicine, 18(12), 2347-2354. doi:10.1007/s10856-007-3135-1M.S. Tkachev, E.S. Melnikov, M.A. Surmeneva, A.A. Sharonova, R.A. Surmenev, O.S. Korneva, I.A. Shulepov, K. Loza, M. Epple, Adhesion properties of a three-layer system based on RF-magnetron sputter deposited calcium-phosphate coating and silver nanoparticles, Proc. - 2016 11th Int. Forum Strateg. Technol. IFOST 2016. (2017) 88–90. doi:https://doi.org/10.1109/IFOST.2016.7884197.E. Mohseni, E. Zalnezhad, a. R. Bushroa, Comparative investigation on the adhesion of hydroxyapatite coating on Ti-6Al-4V implant: A review paper, Int. J. Adhes. Adhes. 48 (2014) 238–257. doi:https://doi.org/10.1016/j.ijadhadh.2013.09.030.Sargeant, A., & Goswami, T. (2007). Hip implants – Paper VI – Ion concentrations. Materials & Design, 28(1), 155-171. doi:10.1016/j.matdes.2005.05.018Qi, J., Yang, Y., Zhou, M., Chen, Z., & Chen, K. (2019). Effect of transition layer on the performance of hydroxyapatite/titanium nitride coating developed on Ti-6Al-4V alloy by magnetron sputtering. Ceramics International, 45(4), 4863-4869. doi:10.1016/j.ceramint.2018.11.183Ghasemi, S., Shanaghi, A., & Chu, P. K. (2017). Nano mechanical and wear properties of multi-layer Ti/TiN coatings deposited on Al 7075 by high-vacuum magnetron sputtering. Thin Solid Films, 638, 96-104. doi:10.1016/j.tsf.2017.07.049Thian, E. S., Huang, J., Barber, Z. H., Best, S. M., & Bonfield, W. (2011). Surface modification of magnetron-sputtered hydroxyapatite thin films via silicon substitution for orthopaedic and dental applications. Surface and Coatings Technology, 205(11), 3472-3477. doi:10.1016/j.surfcoat.2010.12.012Vladescu, A., Birlik, I., Braic, V., Toparli, M., Celik, E., & Ak Azem, F. (2014). Enhancement of the mechanical properties of hydroxyapatite by SiC addition. Journal of the Mechanical Behavior of Biomedical Materials, 40, 362-368. doi:10.1016/j.jmbbm.2014.08.025Azem, F. A., Kiss, A., Birlik, I., Braic, V., Luculescu, C., & Vladescu, A. (2014). The corrosion and bioactivity behavior of SiC doped hydroxyapatite for dental applications. Ceramics International, 40(10), 15881-15887. doi:10.1016/j.ceramint.2014.07.116Ciobanu, C. S., Iconaru, S. L., Le Coustumer, P., Constantin, L. V., & Predoi, D. (2012). Antibacterial activity of silver-doped hydroxyapatite nanoparticles against gram-positive and gram-negative bacteria. Nanoscale Research Letters, 7(1). doi:10.1186/1556-276x-7-324Peetsch, A., Greulich, C., Braun, D., Stroetges, C., Rehage, H., Siebers, B., … Epple, M. (2013). Silver-doped calcium phosphate nanoparticles: Synthesis, characterization, and toxic effects toward mammalian and prokaryotic cells. Colloids and Surfaces B: Biointerfaces, 102, 724-729. doi:10.1016/j.colsurfb.2012.09.040A. Quirama, A.M. Echavarría, J.M. Meza, J. Osorio, G. Bejarano G, Improvement of the mechanical behavior of the calcium phosphate coatings deposited onto Ti6Al4V alloy using an intermediate TiN/TiO2 bilayer, Vacuum. 146 (2017) 22–30. doi:https://doi.org/10.1016/j.vacuum.2017.09.024.Lenis, J. A., Hurtado, F. M., Gómez, M. A., & Bolívar, F. J. (2019). Effect of thermal treatment on structure, phase and mechanical properties of hydroxyapatite thin films grown by RF magnetron sputtering. Thin Solid Films, 669, 571-578. doi:10.1016/j.tsf.2018.11.045Sofronia, A. M., Baies, R., Anghel, E. M., Marinescu, C. A., & Tanasescu, S. (2014). Thermal and structural characterization of synthetic and natural nanocrystalline hydroxyapatite. Materials Science and Engineering: C, 43, 153-163. doi:10.1016/j.msec.2014.07.023Shiri, S., Ashtijoo, P., Odeshi, A., & Yang, Q. (2016). Evaluation of Stoney equation for determining the internal stress of DLC thin films using an optical profiler. Surface and Coatings Technology, 308, 98-100. doi:10.1016/j.surfcoat.2016.07.098Barry, J. N., Cowley, A., McNally, P. J., & Dowling, D. P. (2013). Influence of substrate metal alloy type on the properties of hydroxyapatite coatings deposited using a novel ambient temperature deposition technique. Journal of Biomedical Materials Research Part A, 102(3), 871-879. doi:10.1002/jbm.a.34755Constable, C. P., Yarwood, J., & Münz, W.-D. (1999). Raman microscopic studies of PVD hard coatings. Surface and Coatings Technology, 116-119, 155-159. doi:10.1016/s0257-8972(99)00072-9Cuscó, R., Guitián, F., Aza, S. d., & Artús, L. (1998). Differentiation between hydroxyapatite and β-tricalcium phosphate by means of μ-Raman spectroscopy. Journal of the European Ceramic Society, 18(9), 1301-1305. doi:10.1016/s0955-2219(98)00057-0Ivanova, A. A., Surmeneva, M. A., Grubova, I. Y., Sharonova, A. A., Pichugin, V. F., Chaikina, M. V., … Surmenev, R. A. (2013). Influence of the substrate bias on the stoichiometry and structure of RF-magnetron sputter-deposited silver-containing calcium phosphate coatings. Materialwissenschaft und Werkstofftechnik, 44(2-3), 218-225. doi:10.1002/mawe.201300101Yonggang, Y., Wolke, J. G. C., Yubao, L., & Jansen, J. A. (2007). The influence of discharge power and heat treatment on calcium phosphate coatings prepared by RF magnetron sputtering deposition. Journal of Materials Science: Materials in Medicine, 18(6), 1061-1069. doi:10.1007/s10856-007-0119-0Xin, R., Leng, Y., Chen, J., & Zhang, Q. (2005). A comparative study of calcium phosphate formation on bioceramics in vitro and in vivo. Biomaterials, 26(33), 6477-6486. doi:10.1016/j.biomaterials.2005.04.028Surmeneva, M. A., Tyurin, A. I., Mukhametkaliyev, T. M., Pirozhkova, T. S., Shuvarin, I. A., Syrtanov, M. S., & Surmenev, R. A. (2015). Enhancement of the mechanical properties of AZ31 magnesium alloy via nanostructured hydroxyapatite thin films fabricated via radio-frequency magnetron sputtering. Journal of the Mechanical Behavior of Biomedical Materials, 46, 127-136. doi:10.1016/j.jmbbm.2015.02.025M.A. Surmeneva, M. V. Chaikina, V.I. Zaikovskiy, V.F. Pichugin, V. Buck, O. Prymak, M. Epple, R. a. Surmenev, The structure of an rf-magnetron sputter-deposited silicate-containinghydroxyapatite-based coating investigated by high-resolution techniques, Surf. Coatings Technol. 218 (2013) 39–46. doi:https://doi.org/10.1016/j.surfcoat.2012.12.023.A. Ivanova, M.A. Surmeneva, A.I. Tyurin, T.S. Pirozhkova, I.A. Shuvarin, O. Prymak, M. Epple, M. V Chaikina, R.A. Surmenev, Applied Surface Science Fabrication and physico-mechanical properties of thin magnetron sputter deposited silver-containing hydroxyapatite films, 360 (2016) 929–935.Ding, S.-J., Ju, C.-P., & Lin, J.-H. C. (1999). Characterization of hydroxyapatite and titanium coatings sputtered on Ti-6Al-4V substrate. Journal of Biomedical Materials Research, 44(3), 266-279. doi:10.1002/(sici)1097-4636(19990305)44:33.0.co;2-4A.R.A. Sagari, Ca-P-O thin film preparation, modification and characterisation, Ph.D thesis, University of Jyväskylä, 2011. https://jyx.jyu.fi/bitstream/handle/123456789/37195/Arcot_Rajashekar-Ananda-2011.pdf?sequence=1.Paital, S. R., & Dahotre, N. B. (2009). Calcium phosphate coatings for bio-implant applications: Materials, performance factors, and methodologies. Materials Science and Engineering: R: Reports, 66(1-3), 1-70. doi:10.1016/j.mser.2009.05.001Deligianni, D. (2001). Effect of surface roughness of the titanium alloy Ti–6Al–4V on human bone marrow cell response and on protein adsorption. Biomaterials, 22(11), 1241-1251. doi:10.1016/s0142-9612(00)00274-xEchavarría, A. M., Rico, P., Gómez Ribelles, J. L., Pacha-Olivenza, M. A., Fernández-Calderón, M.-C., & Bejarano-G, G. (2017). Development of a Ta/TaN/TaNx(Ag)y/TaN nanocomposite coating system and bio-response study for biomedical applications. Vacuum, 145, 55-67. doi:10.1016/j.vacuum.2017.08.020García, C. G., Ferrus, L. L., Moratal, D., Pradas, M. M., & Sánchez, M. S. (2009). Poly(L-lactide) Substrates with Tailored Surface Chemistry by Plasma Copolymerisation of Acrylic Monomers. Plasma Processes and Polymers, 6(3), 190-198. doi:10.1002/ppap.200800112Perdok, W. G., Christoffersen, J., & Arends, J. (1987). The thermal lattice expansion of calcium hydroxyapatite. Journal of Crystal Growth, 80(1), 149-154. doi:10.1016/0022-0248(87)90534-3Evans, A. G., Crumley, G. B., & Demaray, R. E. (1983). On the mechanical behavior of brittle coatings and layers. Oxidation of Metals, 20(5-6), 193-216. doi:10.1007/bf00656841ASTM C1624-05, Standard Test Method for Adhesion Strength and Mechanical Failure Modes of, Astm. 05 (2015) 1–29. doi:https://doi.org/10.1520/C1624-05R15.Scope.Yang, Y. ., & Chang, E. (2001). Influence of residual stress on bonding strength and fracture of plasma-sprayed hydroxyapatite coatings on Ti–6Al–4V substrate. Biomaterials, 22(13), 1827-1836. doi:10.1016/s0142-9612(00)00364-1Lenis, J. A., Toro, L. J., & Bolívar, F. J. (2019). Multi-layer bactericidal silver - calcium phosphate coatings obtained by RF magnetron sputtering. Surface and Coatings Technology, 367, 203-211. doi:10.1016/j.surfcoat.2019.03.03