46 research outputs found
Evaluation of the Protein Recognition Properties of Peptide Nucleic Acids
The objective is to evaluate the ability of aminoacyl-tRNA synthetases (aaRS) to recognize the non-standard nucleic acid, PNA (peptide nucleic acid). PNA has immense potential in biomedical applications due to its increased thermostability and nuclease resistance over natural nucleic acids. PNA represents a superior alternative to natural nucleic acids in many biomedical applications due to its specificity, strong binding, and nuclease resistance. This study is the initial data set that indicates PNA are recognized by translation enzymes
The Development of Novel In Vitro Binding Assays to Further Elucidate the Role of tRNAs in Protein Synthesis
Influence of Porcine Plasma Supplementation on Gestating Sow Serum IGF-1 Concentration and Litter Weights
The objective of this pilot study was to determine the effect of dietary porcine plasma on circulating insulin-like growth factor 1 (IGF-1) concentrations in gestating sows and characteristics of their litters. Primiparous and multiparous sows were randomly allocated to two treatment groups of sows fed a basal diet or sows fed the basal diet plus 6 g of porcine plasma throughout gestation. On 4 periods during gestation and farrowing, blood was collected for IGF-1 analysis. After farrowing, gestation length, number born, birth weight, and total litter weight were recorded. There were no three-way or two-way interactions between treatments, day of gestation, or parity for sow IGF-1 concentration (P\u3e0.142). Treatment also did not affect (P=0.117) sow IGF-1 concentration. There were no treatment × parity or treatment effects on litter measures (P=0.170). Feeding porcine plasma at the low level employed in the study did not improve sow IGF-1 or litter measures.
Keywords: Piglets, Fetal Growth, Porcine Plasm
Supporting Data for the Characterization of PNA-DNA Four-Way Junctions
Holliday or DNA four-way junctions (4WJs) are cruciform/bent structures composed of four DNA duplexes. 4WJs are key intermediates in homologous genetic recombination and double-strand break repair. To investigate 4WJs in vitro, junctions are assembled using four asymmetric DNA strands. The presence of four asymmetric strands about the junction branch point eliminates branch migration, and effectively immobilizes the resulting 4WJ. The purpose of these experiments is to show that immobile 4WJs composed of DNA and peptide nucleic acids (PNAs) can be distinguished from contaminating labile nucleic acid structures. These data compare the electrophoretic mobility of hybrid PNA–DNA junctions vs. i) a classic immobile DNA 4WJ, J1 and ii) contaminating nucleic acid structures
Burden of Human papillomavirus (HPV)-related disease and potential impact of HPV vaccines in the Republic of Korea
Background: We aimed to review the burden and the potential impact of human papillomavirus (HPV) vaccines on HPV-related diseases in the Republic of Korea and to discuss cervical cancer prevention practices in this country. Methods: Cancer burden statistics were retrieved from GLOBOCAN-2018 and Statistics Korea. HPV disease burden was assessed via systematic review. Vaccine types relative contribution (RC) was estimated using data from an international project using formalin-fixed paraffin-embedded specimens. Results: Despite a downtrend in cervical cancer in recent years, Korean rates remain high. In contrast, oropharyngeal cancer incidence has gradually increased and other anogenital cancers remain rare. In Korea, HPV prevalence in general population is around 20%. In cervical cancer, RC of HPVs 16/18 (74.0%) increased to 92.0% when including HPVs 31/33/45/52/58. Limited information was available for other HPV-related cancer sites. Regarding prevention, since the inclusion of the HPV vaccine into the National Immunization Program, almost half (49%) of the target cohort in 2016 had received the first dose of vaccine. Further, percentage of women screened with pap has increased from 41.1%-2009 to 53.0%-2016. Conclusions: HPV-related disease burden in Korea is significant. Results suggest that the combination of effective and high coverage HPV vaccination and screening programmes could substantially impact on HPV-related disease in Korea
Niche as a determinant of word fate in online groups
Patterns of word use both reflect and influence a myriad of human activities
and interactions. Like other entities that are reproduced and evolve, words
rise or decline depending upon a complex interplay between {their intrinsic
properties and the environments in which they function}. Using Internet
discussion communities as model systems, we define the concept of a word niche
as the relationship between the word and the characteristic features of the
environments in which it is used. We develop a method to quantify two important
aspects of the size of the word niche: the range of individuals using the word
and the range of topics it is used to discuss. Controlling for word frequency,
we show that these aspects of the word niche are strong determinants of changes
in word frequency. Previous studies have already indicated that word frequency
itself is a correlate of word success at historical time scales. Our analysis
of changes in word frequencies over time reveals that the relative sizes of
word niches are far more important than word frequencies in the dynamics of the
entire vocabulary at shorter time scales, as the language adapts to new
concepts and social groupings. We also distinguish endogenous versus exogenous
factors as additional contributors to the fates of words, and demonstrate the
force of this distinction in the rise of novel words. Our results indicate that
short-term nonstationarity in word statistics is strongly driven by individual
proclivities, including inclinations to provide novel information and to
project a distinctive social identity.Comment: Supporting Information is available here:
http://www.plosone.org/article/fetchSingleRepresentation.action?uri=info:doi/10.1371/journal.pone.0019009.s00
TOI-733 b -- a planet in the small-planet radius valley orbiting a Sun-like star
We report the discovery of a hot ( 1055 K) planet in
the small planet radius valley transiting the Sun-like star TOI-733, as part of
the KESPRINT follow-up program of TESS planets carried out with the HARPS
spectrograph. TESS photometry from sectors 9 and 36 yields an orbital period of
= days and a radius of
= .
Multi-dimensional Gaussian process modelling of the radial velocity
measurements from HARPS and activity indicators, gives a semi-amplitude of
= m s, translating into a planet mass of
= . These
parameters imply that the planet is of moderate density ( =
g cm) and place it in the transition
region between rocky and volatile-rich planets with H/He-dominated envelopes on
the mass-radius diagram. Combining these with stellar parameters and
abundances, we calculate planet interior and atmosphere models, which in turn
suggest that TOI-733 b has a volatile-enriched, most likely secondary outer
envelope, and may represent a highly irradiated ocean world - one of only a few
such planets around G-type stars that are well-characterised.Comment: Accepted for publication in A&
A pair of Sub-Neptunes transiting the bright K-dwarf TOI-1064 characterised with CHEOPS
Funding: TGW, ACC, and KH acknowledge support from STFC consolidated grant numbers ST/R000824/1 and ST/V000861/1, and UKSA grant ST/R003203/1.We report the discovery and characterization of a pair of sub-Neptunes transiting the bright K-dwarf TOI-1064 (TIC 79748331), initially detected in the Transiting Exoplanet Survey Satellite (TESS) photometry. To characterize the system, we performed and retrieved the CHaracterising ExOPlanets Satellite (CHEOPS), TESS, and ground-based photometry, the High Accuracy Radial velocity Planet Searcher (HARPS) high-resolution spectroscopy, and Gemini speckle imaging. We characterize the host star and determine Teff,⋆=4734±67K, R⋆=0.726±0.007R⊙, and M⋆=0.748±0.032M⊙. We present a novel detrending method based on point spread function shape-change modelling and demonstrate its suitability to correct flux variations in CHEOPS data. We confirm the planetary nature of both bodies and find that TOI-1064 b has an orbital period of Pb = 6.44387 ± 0.00003 d, a radius of Rb = 2.59 ± 0.04 R⊕, and a mass of Mb=13.5+1.7−1.8 M⊕, whilst TOI-1064 c has an orbital period of Pc=12.22657+0.00005−0.00004 d, a radius of Rc = 2.65 ± 0.04 R⊕, and a 3σ upper mass limit of 8.5 M⊕. From the high-precision photometry we obtain radius uncertainties of ∼1.6 per cent, allowing us to conduct internal structure and atmospheric escape modelling. TOI-1064 b is one of the densest, well-characterized sub-Neptunes, with a tenuous atmosphere that can be explained by the loss of a primordial envelope following migration through the protoplanetary disc. It is likely that TOI-1064 c has an extended atmosphere due to the tentative low density, however further radial velocities are needed to confirm this scenario and the similar radii, different masses nature of this system. The high-precision data and modelling of TOI-1064 b are important for planets in this region of mass–radius space, and it allow us to identify a trend in bulk density–stellar metallicity for massive sub-Neptunes that may hint at the formation of this population of planets.Publisher PDFPeer reviewe